Q925Z2 (RPOA_RHIME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA-directed RNA polymerase subunit alpha
Short name=RNAP subunit alpha
EC=2.7.7.6

Alternative name(s):
RNA polymerase subunit alpha
Transcriptase subunit alpha

Gene names

Name:	rpoA
Ordered Locus Names:	R01380
ORF Names:	SMc01285

Organism

Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti) [Complete proteome] [HAMAP]

Taxonomic identifier

266834 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium

Protein attributes

Sequence length	336 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. HAMAP MF_00059
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). HAMAP MF_00059
Subunit structure	Homodimer. The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription By similarity.
Domain	The alpha-NTD is essential for RNAP assembly and basal transcription, whereas the alpha-CTD is involved in interaction with transcriptional regulators and with upstream promoter elements By similarity. The alpha-NTD recognizes upstream AT-rich elements and the alpha-CTD interacts with the transcriptional activator Fis in reconstitution assays in E.coli. HAMAP MF_00059
Miscellaneous	Able to replace the endogenous E.coli protein. HAMAP MF_00059
Sequence similarities	Belongs to the RNA polymerase alpha chain family.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW protein dimerization activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 336	336	DNA-directed RNA polymerase subunit alpha HAMAP MF_00059		PRO_0000175365
Regions
	Region	1 – 232	232	Alpha N-terminal domain (alpha-NTD) By similarity
	Region	248 – 336	89	Alpha C-terminal domain (alpha-CTD) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q925Z2 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 70C7CCBBA10CA81D	FASTA	336	37,171
	10 20 30 40 50 60 MIQKNWQELI KPNKVEFASS GRTKATLVAE PLERGFGLTL GNALRRVLLS SLRGAAVTAV 70 80 90 100 110 120 QIDGVLHEFS SIPGVREDVT DIVLNIKEIA IKMDGDDAKR MVVRKQGPGV VTAGDIQTVG 130 140 150 160 170 180 DIEILNPNHV ICTLDEGAEI RMEFTVNNGK GYVPADRNRS EDAPIGLIPV DSLYSPVKKV 190 200 210 220 230 240 SYKVENTREG QVLDYDKLTM SIETDGSVTG EDAIAFAARI LQDQLSVFVN FDEPQKETEE 250 260 270 280 290 300 EAVTELAFNP ALLKKVDELE LSVRSANCLK NDNIVYIGDL IQKTEAEMLR TPNFGRKSLN 310 320 330 EIKEVLASMG LHLGMEVPSW PPENIEDLAK RYEDQY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The composite genome of the legume symbiont Sinorhizobium meliloti." Galibert F., Finan T.M., Long S.R., Puehler A., Abola P., Ampe F., Barloy-Hubler F., Barnett M.J., Becker A., Boistard P., Bothe G., Boutry M., Bowser L., Buhrmester J., Cadieu E., Capela D., Chain P., Cowie A. , Davis R.W., Dreano S., Federspiel N.A., Fisher R.F., Gloux S., Godrie T., Goffeau A., Golding B., Gouzy J., Gurjal M., Hernandez-Lucas I., Hong A., Huizar L., Hyman R.W., Jones T., Kahn D., Kahn M.L., Kalman S., Keating D.H., Kiss E., Komp C., Lelaure V., Masuy D., Palm C., Peck M.C., Pohl T.M., Portetelle D., Purnelle B., Ramsperger U., Surzycki R., Thebault P., Vandenbol M., Vorhoelter F.J., Weidner S., Wells D.H., Wong K., Yeh K.-C., Batut J. Science 293:668-672(2001) [PubMed: 11474104] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1021.
[2]	"The RNA polymerase alpha subunit from Sinorhizobium meliloti can assemble with RNA polymerase subunits from Escherichia coli and function in basal and activated transcription both in vivo and in vitro." Peck M.C., Gaal T., Fisher R.F., Gourse R.L., Long S.R. J. Bacteriol. 184:3808-3814(2002) [PubMed: 12081950] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION IN E.COLI. Strain: 1021.
[3]	"Analysis of the chromosome sequence of the legume symbiont Sinorhizobium meliloti strain 1021." Capela D., Barloy-Hubler F., Gouzy J., Bothe G., Ampe F., Batut J., Boistard P., Becker A., Boutry M., Cadieu E., Dreano S., Gloux S., Godrie T., Goffeau A., Kahn D., Kiss E., Lelaure V., Masuy D. , Pohl T., Portetelle D., Puehler A., Purnelle B., Ramsperger U., Renard C., Thebault P., Vandenbol M., Weidner S., Galibert F. Proc. Natl. Acad. Sci. U.S.A. 98:9877-9882(2001) [PubMed: 11481430] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1021.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF317474 Genomic DNA. Translation: AAL26901.1. AL591688 Genomic DNA. Translation: CAC45959.1.
RefSeq	NP_385486.1. NC_003047.1.
3D structure databases
ProteinModelPortal	Q925Z2.
SMR	Q925Z2. Positions 248-327.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1233032.
GenomeReviews	Gene locus R01380 in contig AL591688_GR.
KEGG	sme:SMc01285.
NMPDR	fig|266834.1.peg.2674.
PATRIC	23632089. VBISinMel96828_2797.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG430844.
OMA	VRSHNCL.
ProtClustDB	PRK05182.
Enzyme and pathway databases
BioCyc	SMEL266834:SMC01285-MONOMER.
Family and domain databases
HAMAP	MF_00059. RNApol_bact_RpoA. [Tree]
InterPro	IPR011261. DNA-dir_RNA_pol_dimersation. IPR011262. DNA-dir_RNA_pol_insert. IPR009025. DNA-dir_RNA_pol_RBP11-like. IPR011263. DNA-dir_RNA_pol_RpoA/D/Rpb3. IPR011773. DNA-dir_RpoA. IPR011260. RNAP_asu_C. [Graphical view]
Gene3D	G3DSA:2.170.120.12. RNAP_insert. 1 hit.
KO	K03040.
Pfam	PF01000. RNA_pol_A_bac. 1 hit. PF03118. RNA_pol_A_CTD. 1 hit. PF01193. RNA_pol_L. 1 hit. [Graphical view]
ProDom	PD001179. RNAP_asu_C. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00662. RPOLD. 1 hit. [Graphical view]
SUPFAM	SSF47789. RNAP_alpha_C. 1 hit. SSF56553. RNAP_insert. 1 hit. SSF55257. RNAP_RBP11-like. 1 hit.
TIGRFAMs	TIGR02027. RpoA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RPOA_RHIME

Accession

Primary (citable) accession number: Q925Z2

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 27, 2002
Last sequence update:	December 1, 2001
Last modified:	January 25, 2012
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program