Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

ER degradation-enhancing alpha-mannosidase-like protein 1

Gene

Edem1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Extracts misfolded glycoproteins, but not glycoproteins undergoing productive folding, from the calnexin cycle. It is directly involved in endoplasmic reticulum-associated degradation (ERAD) and targets misfolded glycoproteins for degradation in an N-glycan-independent manner, probably by forming a complex with SEL1L. It has low mannosidase activity, catalyzing mannose trimming from Man8GlcNAc2 to Man7GlcNAc2.By similarity1 Publication

GO - Molecular functioni

  • calcium ion binding Source: InterPro
  • mannosyl-oligosaccharide 1,2-alpha-mannosidase activity Source: ParkinsonsUK-UCL
  • misfolded protein binding Source: UniProtKB

GO - Biological processi

Keywordsi

Biological processUnfolded protein response

Enzyme and pathway databases

ReactomeiR-MMU-901032 ER Quality Control Compartment (ERQC)

Protein family/group databases

CAZyiGH47 Glycoside Hydrolase Family 47

Names & Taxonomyi

Protein namesi
Recommended name:
ER degradation-enhancing alpha-mannosidase-like protein 1
Gene namesi
Name:Edem1
Synonyms:Edem
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:2180139 Edem1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 4CytoplasmicSequence analysis4
Transmembranei5 – 25Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini26 – 652LumenalSequence analysisAdd BLAST627

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002103221 – 652ER degradation-enhancing alpha-mannosidase-like protein 1Add BLAST652

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi176N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi193N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi294N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi337N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

EPDiQ925U4
MaxQBiQ925U4
PaxDbiQ925U4
PRIDEiQ925U4

PTM databases

iPTMnetiQ925U4
PhosphoSitePlusiQ925U4

Expressioni

Gene expression databases

BgeeiENSMUSG00000030104
CleanExiMM_EDEM1
ExpressionAtlasiQ925U4 baseline and differential
GenevisibleiQ925U4 MM

Interactioni

Subunit structurei

Interacts with DERL2 and DERL3. Binds to SEL1L (By similarity). Interacts with DNAJC10.By similarity2 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi228670, 2 interactors
IntActiQ925U4, 8 interactors
STRINGi10090.ENSMUSP00000086565

Structurei

3D structure databases

ProteinModelPortaliQ925U4
SMRiQ925U4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 47 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2429 Eukaryota
ENOG410XS6F LUCA
GeneTreeiENSGT00530000063069
HOVERGENiHBG051158
InParanoidiQ925U4
KOiK10084
OMAiKQPFGDM
OrthoDBiEOG091G04YS
PhylomeDBiQ925U4
TreeFamiTF300807

Family and domain databases

Gene3Di1.50.10.10, 1 hit
InterProiView protein in InterPro
IPR012341 6hp_glycosidase-like_sf
IPR001382 Glyco_hydro_47
IPR036026 Seven-hairpin_glycosidases
PfamiView protein in Pfam
PF01532 Glyco_hydro_47, 1 hit
PRINTSiPR00747 GLYHDRLASE47
SUPFAMiSSF48225 SSF48225, 1 hit

Sequencei

Sequence statusi: Complete.

Q925U4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQWRALVLGL VLLRLGLHAV LWLVFGLGPS MGFYQRFPLS FGFQRLRDPD
60 70 80 90 100
GSGPVGPPGG PAWLHRPRRG TEGRLETPPE PGPTPGPGVC GPAHWGYALG
110 120 130 140 150
GGGCGPDEYE RRYSGAFPPQ LRAQMRDLAR GMFVFGYDNY MAHAFPQDEL
160 170 180 190 200
NPIYCRGRGP DRGDPSNLNI NDVLGNYSLT LVDALDTLAI MGNSSEFQKA
210 220 230 240 250
VKLVINTVSF DKDSTVQVFE ATIRVLGSLL SAHRIITDSK QPFGDMTIED
260 270 280 290 300
YDNELLYMAH DLAVRLLPAF ENTKTGIPYP RVNLKTGVPP DSNNETCTAG
310 320 330 340 350
AGSLLVEFGI LSRLLGDSTF EWVARRAVKA LWNLRSNDTG LLGNVVNIQT
360 370 380 390 400
GHWVGKQSGL GAGLDSFYEY LLKSYILFGE KEDLEMFNAA YQSIQSYLRR
410 420 430 440 450
GREACNEGEG DPPLYVNVNM FSGQLMNTWI DSLQAFFPGL QVLIGDVEDA
460 470 480 490 500
ICLHAFYYAI WKRYGALPER YNWQLQAPDV LFYPLRPELV ESTYLLYQAT
510 520 530 540 550
KNPFYLHVGM DILQSLEKYT KVKCGYATLH HVIDKSKEDR MESFFLSETC
560 570 580 590 600
KYLYLLFDEE NPVHKSGTRY MFTTEGHIIS VDKRLRELPW KEFFSEDGER
610 620 630 640 650
DQEEKFVHRP KSQELRVINS SSNCNRVPDE RRYSLPLKSI YMRQIDQMVG

LI
Length:652
Mass (Da):73,701
Last modified:December 1, 2001 - v1
Checksum:i3BFC9A874B0E537E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB042828 mRNA Translation: BAB55676.1
AK082920 mRNA Translation: BAC38688.1
BC023237 mRNA Translation: AAH23237.2
CCDSiCCDS20402.1
RefSeqiNP_619618.1, NM_138677.2
UniGeneiMm.21596
Mm.276305

Genome annotation databases

EnsembliENSMUST00000089162; ENSMUSP00000086565; ENSMUSG00000030104
GeneIDi192193
KEGGimmu:192193
UCSCiuc009ddq.1 mouse

Similar proteinsi

Entry informationi

Entry nameiEDEM1_MOUSE
AccessioniPrimary (citable) accession number: Q925U4
Secondary accession number(s): Q8CFM1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 31, 2003
Last sequence update: December 1, 2001
Last modified: March 28, 2018
This is version 130 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health