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Protein

ER degradation-enhancing alpha-mannosidase-like protein 1

Gene

Edem1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Extracts misfolded glycoproteins, but not glycoproteins undergoing productive folding, from the calnexin cycle. It is directly involved in endoplasmic reticulum-associated degradation (ERAD) and targets misfolded glycoproteins for degradation in an N-glycan-independent manner, probably by forming a complex with SEL1L. It has low mannosidase activity, catalyzing mannose trimming from Man8GlcNAc2 to Man7GlcNAc2.By similarity1 Publication

GO - Molecular functioni

  • calcium ion binding Source: InterPro
  • glycoprotein binding Source: ParkinsonsUK-UCL
  • mannosyl-oligosaccharide 1,2-alpha-mannosidase activity Source: ParkinsonsUK-UCL
  • misfolded protein binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Unfolded protein response

Enzyme and pathway databases

ReactomeiR-MMU-901032. ER Quality Control Compartment (ERQC).

Protein family/group databases

CAZyiGH47. Glycoside Hydrolase Family 47.

Names & Taxonomyi

Protein namesi
Recommended name:
ER degradation-enhancing alpha-mannosidase-like protein 1
Gene namesi
Name:Edem1
Synonyms:Edem
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:2180139. Edem1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 44CytoplasmicSequence analysis
Transmembranei5 – 2521Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini26 – 652627LumenalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 652652ER degradation-enhancing alpha-mannosidase-like protein 1PRO_0000210322Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi176 – 1761N-linked (GlcNAc...)Sequence analysis
Glycosylationi193 – 1931N-linked (GlcNAc...)Sequence analysis
Glycosylationi294 – 2941N-linked (GlcNAc...)Sequence analysis
Glycosylationi337 – 3371N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

EPDiQ925U4.
MaxQBiQ925U4.
PaxDbiQ925U4.
PRIDEiQ925U4.

PTM databases

iPTMnetiQ925U4.
PhosphoSiteiQ925U4.

Expressioni

Gene expression databases

BgeeiENSMUSG00000030104.
CleanExiMM_EDEM1.
ExpressionAtlasiQ925U4. baseline and differential.
GenevisibleiQ925U4. MM.

Interactioni

Subunit structurei

Interacts with DERL2 and DERL3. Binds to SEL1L (By similarity). Interacts with DNAJC10.By similarity2 Publications

GO - Molecular functioni

  • glycoprotein binding Source: ParkinsonsUK-UCL
  • misfolded protein binding Source: UniProtKB

Protein-protein interaction databases

BioGridi228670. 2 interactions.
IntActiQ925U4. 8 interactions.
STRINGi10090.ENSMUSP00000086565.

Structurei

3D structure databases

ProteinModelPortaliQ925U4.
SMRiQ925U4. Positions 118-559.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 47 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2429. Eukaryota.
ENOG410XS6F. LUCA.
GeneTreeiENSGT00530000063069.
HOVERGENiHBG051158.
InParanoidiQ925U4.
KOiK10084.
OMAiELLYMAH.
OrthoDBiEOG091G04YS.
PhylomeDBiQ925U4.
TreeFamiTF300807.

Family and domain databases

Gene3Di1.50.10.50. 1 hit.
InterProiIPR001382. Glyco_hydro_47.
[Graphical view]
PfamiPF01532. Glyco_hydro_47. 1 hit.
[Graphical view]
PRINTSiPR00747. GLYHDRLASE47.
SUPFAMiSSF48225. SSF48225. 1 hit.

Sequencei

Sequence statusi: Complete.

Q925U4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQWRALVLGL VLLRLGLHAV LWLVFGLGPS MGFYQRFPLS FGFQRLRDPD
60 70 80 90 100
GSGPVGPPGG PAWLHRPRRG TEGRLETPPE PGPTPGPGVC GPAHWGYALG
110 120 130 140 150
GGGCGPDEYE RRYSGAFPPQ LRAQMRDLAR GMFVFGYDNY MAHAFPQDEL
160 170 180 190 200
NPIYCRGRGP DRGDPSNLNI NDVLGNYSLT LVDALDTLAI MGNSSEFQKA
210 220 230 240 250
VKLVINTVSF DKDSTVQVFE ATIRVLGSLL SAHRIITDSK QPFGDMTIED
260 270 280 290 300
YDNELLYMAH DLAVRLLPAF ENTKTGIPYP RVNLKTGVPP DSNNETCTAG
310 320 330 340 350
AGSLLVEFGI LSRLLGDSTF EWVARRAVKA LWNLRSNDTG LLGNVVNIQT
360 370 380 390 400
GHWVGKQSGL GAGLDSFYEY LLKSYILFGE KEDLEMFNAA YQSIQSYLRR
410 420 430 440 450
GREACNEGEG DPPLYVNVNM FSGQLMNTWI DSLQAFFPGL QVLIGDVEDA
460 470 480 490 500
ICLHAFYYAI WKRYGALPER YNWQLQAPDV LFYPLRPELV ESTYLLYQAT
510 520 530 540 550
KNPFYLHVGM DILQSLEKYT KVKCGYATLH HVIDKSKEDR MESFFLSETC
560 570 580 590 600
KYLYLLFDEE NPVHKSGTRY MFTTEGHIIS VDKRLRELPW KEFFSEDGER
610 620 630 640 650
DQEEKFVHRP KSQELRVINS SSNCNRVPDE RRYSLPLKSI YMRQIDQMVG

LI
Length:652
Mass (Da):73,701
Last modified:December 1, 2001 - v1
Checksum:i3BFC9A874B0E537E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB042828 mRNA. Translation: BAB55676.1.
AK082920 mRNA. Translation: BAC38688.1.
BC023237 mRNA. Translation: AAH23237.2.
CCDSiCCDS20402.1.
RefSeqiNP_619618.1. NM_138677.2.
UniGeneiMm.21596.

Genome annotation databases

EnsembliENSMUST00000089162; ENSMUSP00000086565; ENSMUSG00000030104.
GeneIDi192193.
KEGGimmu:192193.
UCSCiuc009ddq.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB042828 mRNA. Translation: BAB55676.1.
AK082920 mRNA. Translation: BAC38688.1.
BC023237 mRNA. Translation: AAH23237.2.
CCDSiCCDS20402.1.
RefSeqiNP_619618.1. NM_138677.2.
UniGeneiMm.21596.

3D structure databases

ProteinModelPortaliQ925U4.
SMRiQ925U4. Positions 118-559.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi228670. 2 interactions.
IntActiQ925U4. 8 interactions.
STRINGi10090.ENSMUSP00000086565.

Protein family/group databases

CAZyiGH47. Glycoside Hydrolase Family 47.

PTM databases

iPTMnetiQ925U4.
PhosphoSiteiQ925U4.

Proteomic databases

EPDiQ925U4.
MaxQBiQ925U4.
PaxDbiQ925U4.
PRIDEiQ925U4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000089162; ENSMUSP00000086565; ENSMUSG00000030104.
GeneIDi192193.
KEGGimmu:192193.
UCSCiuc009ddq.1. mouse.

Organism-specific databases

CTDi9695.
MGIiMGI:2180139. Edem1.

Phylogenomic databases

eggNOGiKOG2429. Eukaryota.
ENOG410XS6F. LUCA.
GeneTreeiENSGT00530000063069.
HOVERGENiHBG051158.
InParanoidiQ925U4.
KOiK10084.
OMAiELLYMAH.
OrthoDBiEOG091G04YS.
PhylomeDBiQ925U4.
TreeFamiTF300807.

Enzyme and pathway databases

ReactomeiR-MMU-901032. ER Quality Control Compartment (ERQC).

Miscellaneous databases

ChiTaRSiEdem1. mouse.
PROiQ925U4.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000030104.
CleanExiMM_EDEM1.
ExpressionAtlasiQ925U4. baseline and differential.
GenevisibleiQ925U4. MM.

Family and domain databases

Gene3Di1.50.10.50. 1 hit.
InterProiIPR001382. Glyco_hydro_47.
[Graphical view]
PfamiPF01532. Glyco_hydro_47. 1 hit.
[Graphical view]
PRINTSiPR00747. GLYHDRLASE47.
SUPFAMiSSF48225. SSF48225. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiEDEM1_MOUSE
AccessioniPrimary (citable) accession number: Q925U4
Secondary accession number(s): Q8CFM1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2003
Last sequence update: December 1, 2001
Last modified: September 7, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.