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Q925U2 (LTC4S_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leukotriene C4 synthase

Short name=LTC4 synthase
EC=4.4.1.20
Alternative name(s):
Leukotriene-C(4) synthase
Gene names
Name:Ltc4s
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length150 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the conjugation of leukotriene A4 with reduced glutathione to form leukotriene C4. Ref.1

Catalytic activity

Leukotriene C4 = leukotriene A4 + glutathione.

Subunit structure

Homotrimer. Interacts with ALOX5AP and ALOX5 By similarity.

Subcellular location

Nucleus outer membrane; Multi-pass membrane protein By similarity. Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.

Induction

Up-regulated by all-trans retinoic acid. Ref.1

Sequence similarities

Belongs to the MAPEG family.

Ontologies

Keywords
   Biological processLeukotriene biosynthesis
   Cellular componentEndoplasmic reticulum
Membrane
Nucleus
   DomainTransmembrane
Transmembrane helix
   Molecular functionLyase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcellular response to lipopolysaccharide

Inferred from expression pattern PubMed 9794912. Source: RGD

cellular response to vitamin A

Inferred from expression pattern PubMed 12767051. Source: RGD

leukotriene biosynthetic process

Inferred from direct assay PubMed 14637132. Source: RGD

oxidation-reduction process

Inferred from Biological aspect of Ancestor. Source: GOC

response to axon injury

Inferred from expression pattern PubMed 19908283. Source: RGD

response to drug

Inferred from expression pattern PubMed 12767051. Source: RGD

response to inorganic substance

Inferred from expression pattern PubMed 17496435. Source: RGD

response to lipopolysaccharide

Inferred from expression pattern PubMed 15619010. Source: RGD

   Cellular_componentendoplasmic reticulum

Inferred from Biological aspect of Ancestor. Source: RefGenome

endoplasmic reticulum membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

intracellular membrane-bounded organelle

Inferred from direct assay PubMed 17194456. Source: RGD

nuclear envelope

Inferred from Biological aspect of Ancestor. Source: RefGenome

nuclear outer membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionenzyme activator activity

Inferred from electronic annotation. Source: InterPro

glutathione binding

Inferred from direct assay PubMed 18440824. Source: RGD

glutathione peroxidase activity

Inferred from Biological aspect of Ancestor. Source: RefGenome

glutathione transferase activity

Inferred from Biological aspect of Ancestor. Source: RefGenome

leukotriene-C4 synthase activity

Inferred from direct assay PubMed 12767051PubMed 17194456. Source: RGD

protein heterodimerization activity

Inferred from physical interaction PubMed 15084748. Source: RGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 150150Leukotriene C4 synthase
PRO_0000378088

Regions

Topological domain1 – 66Cytoplasmic By similarity
Transmembrane7 – 2721Helical; By similarity
Topological domain28 – 4821Lumenal By similarity
Transmembrane49 – 6921Helical; By similarity
Topological domain70 – 734Cytoplasmic By similarity
Transmembrane74 – 9421Helical; By similarity
Topological domain95 – 10410Lumenal By similarity
Transmembrane105 – 12420Helical; By similarity
Topological domain125 – 15026Cytoplasmic By similarity
Region51 – 555Glutathione binding By similarity
Region58 – 592Glutathione binding By similarity
Region93 – 975Glutathione binding By similarity

Sites

Active site311Proton donor By similarity
Active site1041Proton acceptor By similarity
Binding site301Glutathione By similarity

Sequences

Sequence LengthMass (Da)Tools
Q925U2 [UniParc].

Last modified June 1, 2002. Version 2.
Checksum: 7C458B43F4B15D4C

FASTA15016,836
        10         20         30         40         50         60 
MKEETALLAT VTLVGVLLQA YFSLQVISAR RTFHVSPPLT SGPPEFERVF RAQVNCSEYF 

        70         80         90        100        110        120 
PLFLATLWVA GIFFHEGAAA LCGLFYLFAR LRYFQGYARS AQHRLDPLYA SARALWLLVA 

       130        140        150 
MAALGLLVHF LPGTLRAALF RWLQVLLPMA 

« Hide

References

[1]"cDNA cloning and expression of rat leukotriene C(4) synthase: elevated expression in rat basophilic leukemia-1 cells after treatment with retinoic acid."
Abe M., Shibata K., Saruwatar S., Soeda S., Shimeno H., Katsuragi T.
Prostaglandins Leukot. Essent. Fatty Acids 67:319-326(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INDUCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB048790 mRNA. Translation: BAB58882.2.
RefSeqNP_446091.2. NM_053639.2.
UniGeneRn.38594.

3D structure databases

ProteinModelPortalQ925U2.
SMRQ925U2. Positions 2-111.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000004359.

Chemistry

ChEMBLCHEMBL2172.

Proteomic databases

PRIDEQ925U2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID114097.
KEGGrno:114097.
UCSCRGD:620677. rat.

Organism-specific databases

CTD4056.
RGD620677. Ltc4s.

Phylogenomic databases

eggNOGNOG146874.
HOGENOMHOG000116372.
InParanoidQ925U2.
KOK00807.
PhylomeDBQ925U2.

Gene expression databases

GenevestigatorQ925U2.

Family and domain databases

Gene3D1.20.120.550. 1 hit.
InterProIPR001446. 5_LipOase_AP.
IPR018295. FLAP/GST2/LTC4S_CS.
IPR023352. MAPEG-like_dom.
IPR001129. Membr-assoc_MAPEG.
[Graphical view]
PfamPF01124. MAPEG. 1 hit.
[Graphical view]
PRINTSPR00488. 5LPOXGNASEAP.
PROSITEPS01297. FLAP_GST2_LTC4S. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio618245.
PROQ925U2.

Entry information

Entry nameLTC4S_RAT
AccessionPrimary (citable) accession number: Q925U2
Entry history
Integrated into UniProtKB/Swiss-Prot: June 16, 2009
Last sequence update: June 1, 2002
Last modified: April 16, 2014
This is version 65 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families