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Protein

Leukotriene C4 synthase

Gene

Ltc4s

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the conjugation of leukotriene A4 with reduced glutathione to form leukotriene C4.1 Publication

Catalytic activityi

Leukotriene C4 = leukotriene A4 + glutathione.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei30 – 301GlutathioneBy similarity
Active sitei31 – 311Proton donorBy similarity
Active sitei104 – 1041Proton acceptorBy similarity

GO - Molecular functioni

  1. enzyme activator activity Source: InterPro
  2. glutathione binding Source: RGD
  3. glutathione peroxidase activity Source: GO_Central
  4. glutathione transferase activity Source: GO_Central
  5. leukotriene-C4 synthase activity Source: RGD
  6. protein heterodimerization activity Source: RGD

GO - Biological processi

  1. cellular response to lipopolysaccharide Source: RGD
  2. cellular response to vitamin A Source: RGD
  3. leukotriene biosynthetic process Source: RGD
  4. oxidation-reduction process Source: GOC
  5. response to axon injury Source: RGD
  6. response to drug Source: RGD
  7. response to inorganic substance Source: RGD
  8. response to lipopolysaccharide Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Leukotriene biosynthesis

Enzyme and pathway databases

BRENDAi4.4.1.20. 5301.

Names & Taxonomyi

Protein namesi
Recommended name:
Leukotriene C4 synthase (EC:4.4.1.20)
Short name:
LTC4 synthase
Alternative name(s):
Leukotriene-C(4) synthase
Gene namesi
Name:Ltc4s
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620677. Ltc4s.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 66CytoplasmicBy similarity
Transmembranei7 – 2721HelicalBy similarityAdd
BLAST
Topological domaini28 – 4821LumenalBy similarityAdd
BLAST
Transmembranei49 – 6921HelicalBy similarityAdd
BLAST
Topological domaini70 – 734CytoplasmicBy similarity
Transmembranei74 – 9421HelicalBy similarityAdd
BLAST
Topological domaini95 – 10410LumenalBy similarity
Transmembranei105 – 12420HelicalBy similarityAdd
BLAST
Topological domaini125 – 15026CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum Source: GO_Central
  2. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  3. integral component of membrane Source: UniProtKB-KW
  4. intracellular membrane-bounded organelle Source: RGD
  5. nuclear envelope Source: GO_Central
  6. nuclear outer membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 150150Leukotriene C4 synthasePRO_0000378088Add
BLAST

Proteomic databases

PRIDEiQ925U2.

Expressioni

Inductioni

Up-regulated by all-trans retinoic acid.1 Publication

Gene expression databases

GenevestigatoriQ925U2.

Interactioni

Subunit structurei

Homotrimer. Interacts with ALOX5AP and ALOX5 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000004359.

Structurei

3D structure databases

ProteinModelPortaliQ925U2.
SMRiQ925U2. Positions 2-111.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni51 – 555Glutathione bindingBy similarity
Regioni58 – 592Glutathione bindingBy similarity
Regioni93 – 975Glutathione bindingBy similarity

Sequence similaritiesi

Belongs to the MAPEG family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG146874.
HOGENOMiHOG000116372.
InParanoidiQ925U2.
KOiK00807.
PhylomeDBiQ925U2.

Family and domain databases

Gene3Di1.20.120.550. 1 hit.
InterProiIPR001446. 5_LipOase_AP.
IPR018295. FLAP/GST2/LTC4S_CS.
IPR023352. MAPEG-like_dom.
IPR001129. Membr-assoc_MAPEG.
[Graphical view]
PfamiPF01124. MAPEG. 1 hit.
[Graphical view]
PRINTSiPR00488. 5LPOXGNASEAP.
PROSITEiPS01297. FLAP_GST2_LTC4S. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q925U2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKEETALLAT VTLVGVLLQA YFSLQVISAR RTFHVSPPLT SGPPEFERVF
60 70 80 90 100
RAQVNCSEYF PLFLATLWVA GIFFHEGAAA LCGLFYLFAR LRYFQGYARS
110 120 130 140 150
AQHRLDPLYA SARALWLLVA MAALGLLVHF LPGTLRAALF RWLQVLLPMA
Length:150
Mass (Da):16,836
Last modified:May 31, 2002 - v2
Checksum:i7C458B43F4B15D4C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB048790 mRNA. Translation: BAB58882.2.
RefSeqiNP_446091.2. NM_053639.2.
UniGeneiRn.38594.

Genome annotation databases

GeneIDi114097.
KEGGirno:114097.
UCSCiRGD:620677. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB048790 mRNA. Translation: BAB58882.2.
RefSeqiNP_446091.2. NM_053639.2.
UniGeneiRn.38594.

3D structure databases

ProteinModelPortaliQ925U2.
SMRiQ925U2. Positions 2-111.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000004359.

Chemistry

ChEMBLiCHEMBL2172.

Proteomic databases

PRIDEiQ925U2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi114097.
KEGGirno:114097.
UCSCiRGD:620677. rat.

Organism-specific databases

CTDi4056.
RGDi620677. Ltc4s.

Phylogenomic databases

eggNOGiNOG146874.
HOGENOMiHOG000116372.
InParanoidiQ925U2.
KOiK00807.
PhylomeDBiQ925U2.

Enzyme and pathway databases

BRENDAi4.4.1.20. 5301.

Miscellaneous databases

NextBioi618245.
PROiQ925U2.

Gene expression databases

GenevestigatoriQ925U2.

Family and domain databases

Gene3Di1.20.120.550. 1 hit.
InterProiIPR001446. 5_LipOase_AP.
IPR018295. FLAP/GST2/LTC4S_CS.
IPR023352. MAPEG-like_dom.
IPR001129. Membr-assoc_MAPEG.
[Graphical view]
PfamiPF01124. MAPEG. 1 hit.
[Graphical view]
PRINTSiPR00488. 5LPOXGNASEAP.
PROSITEiPS01297. FLAP_GST2_LTC4S. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "cDNA cloning and expression of rat leukotriene C(4) synthase: elevated expression in rat basophilic leukemia-1 cells after treatment with retinoic acid."
    Abe M., Shibata K., Saruwatar S., Soeda S., Shimeno H., Katsuragi T.
    Prostaglandins Leukot. Essent. Fatty Acids 67:319-326(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INDUCTION.

Entry informationi

Entry nameiLTC4S_RAT
AccessioniPrimary (citable) accession number: Q925U2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 15, 2009
Last sequence update: May 31, 2002
Last modified: March 31, 2015
This is version 70 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.