Q925U2 (LTC4S_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Leukotriene C4 synthase
Short name=LTC4 synthase
EC=4.4.1.20

Alternative name(s):
Leukotriene-C(4) synthase

Gene names

Name:

Ltc4s

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	150 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the conjugation of leukotriene A4 with reduced glutathione to form leukotriene C4. Ref.1
Catalytic activity	Leukotriene C4 = leukotriene A4 + glutathione.
Subunit structure	Homotrimer. Interacts with ALOX5AP and ALOX5 By similarity.
Subcellular location	Nucleus outer membrane; Multi-pass membrane protein By similarity. Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Induction	Up-regulated by all-trans retinoic acid. Ref.1
Sequence similarities	Belongs to the MAPEG family.

Ontologies

Keywords
Biological process	Leukotriene biosynthesis
Cellular component	Endoplasmic reticulum Membrane Nucleus
Domain	Transmembrane Transmembrane helix
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular response to lipopolysaccharide Inferred from expression pattern PubMed 9794912. Source: RGD cellular response to vitamin A Inferred from expression pattern PubMed 12767051. Source: RGD glutathione biosynthetic process Inferred from Biological aspect of Ancestor. Source: RefGenome leukotriene biosynthetic process Inferred from direct assay PubMed 14637132. Source: RGD positive regulation of catalytic activity Inferred from electronic annotation. Source: GOC response to axon injury Inferred from expression pattern PubMed 19908283. Source: RGD response to drug Inferred from expression pattern PubMed 12767051. Source: RGD response to inorganic substance Inferred from expression pattern PubMed 17496435. Source: RGD
Cellular_component	endoplasmic reticulum Inferred from Biological aspect of Ancestor. Source: RefGenome endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW nuclear envelope Inferred from Biological aspect of Ancestor. Source: RefGenome nuclear outer membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	enzyme activator activity Inferred from electronic annotation. Source: InterPro glutathione binding Inferred from direct assay PubMed 18440824. Source: RGD glutathione peroxidase activity Inferred from Biological aspect of Ancestor. Source: RefGenome glutathione transferase activity Inferred from Biological aspect of Ancestor. Source: RefGenome leukotriene-C4 synthase activity Inferred from direct assay PubMed 12767051PubMed 17194456. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 150	150	Leukotriene C4 synthase		PRO_0000378088
Regions
	Topological domain	1 – 6	6	Cytoplasmic By similarity
	Transmembrane	7 – 27	21	Helical; By similarity
	Topological domain	28 – 48	21	Lumenal By similarity
	Transmembrane	49 – 69	21	Helical; By similarity
	Topological domain	70 – 73	4	Cytoplasmic By similarity
	Transmembrane	74 – 94	21	Helical; By similarity
	Topological domain	95 – 104	10	Lumenal By similarity
	Transmembrane	105 – 124	20	Helical; By similarity
	Topological domain	125 – 150	26	Cytoplasmic By similarity
	Region	51 – 55	5	Glutathione binding By similarity
	Region	58 – 59	2	Glutathione binding By similarity
	Region	93 – 97	5	Glutathione binding By similarity
Sites
	Active site	31	1	Proton donor By similarity
	Active site	104	1	Proton acceptor By similarity
	Binding site	30	1	Glutathione By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q925U2 [UniParc]. Last modified June 1, 2002. Version 2. Checksum: 7C458B43F4B15D4C	FASTA	150	16,836
	10 20 30 40 50 60 MKEETALLAT VTLVGVLLQA YFSLQVISAR RTFHVSPPLT SGPPEFERVF RAQVNCSEYF 70 80 90 100 110 120 PLFLATLWVA GIFFHEGAAA LCGLFYLFAR LRYFQGYARS AQHRLDPLYA SARALWLLVA 130 140 150 MAALGLLVHF LPGTLRAALF RWLQVLLPMA

References

[1]	"cDNA cloning and expression of rat leukotriene C(4) synthase: elevated expression in rat basophilic leukemia-1 cells after treatment with retinoic acid." Abe M., Shibata K., Saruwatar S., Soeda S., Shimeno H., Katsuragi T. Prostaglandins Leukot. Essent. Fatty Acids 67:319-326(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INDUCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB048790 mRNA. Translation: BAB58882.2.
IPI	IPI00206120.
RefSeq	NP_446091.2. NM_053639.2.
UniGene	Rn.38594.
3D structure databases
ProteinModelPortal	Q925U2.
SMR	Q925U2. Positions 2-111.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000004359.
Proteomic databases
PRIDE	Q925U2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	114097.
KEGG	rno:114097.
UCSC	RGD:620677. rat.
Organism-specific databases
CTD	4056.
RGD	620677. Ltc4s.
Phylogenomic databases
eggNOG	NOG146874.
HOGENOM	HOG000116372.
InParanoid	Q925U2.
KO	K00807.
OrthoDB	EOG4V1729.
Gene expression databases
Genevestigator	Q925U2.
Family and domain databases
Gene3D	1.20.120.550. 1 hit.
InterPro	IPR001446. 5_LipOase_AP. IPR018295. FLAP/GST2/LTC4S_CS. IPR023352. MAPEG-like_dom. IPR001129. Membr-assoc_MAPEG. [Graphical view]
Pfam	PF01124. MAPEG. 1 hit. [Graphical view]
PRINTS	PR00488. 5LPOXGNASEAP.
PROSITE	PS01297. FLAP_GST2_LTC4S. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChEMBL	CHEMBL2172.
NextBio	618245.

Entry information

Entry name

LTC4S_RAT

Accession

Primary (citable) accession number: Q925U2

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 16, 2009
Last sequence update:	June 1, 2002
Last modified:	April 3, 2013
This is version 58 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program