Q925Q8 (DACH2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dachshund homolog 2
Short name=Dach2

Gene names

Name:

Dach2

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	634 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transcription factor that is involved in regulation of organogenesis. Seems to be a regulator for SIX1 and SIX6. Seems to act as a corepressor of SIX6 in regulating proliferation by directly repressing cyclin-dependent kinase inhibitors, including the p27Kip1 promoter. Is recruited with SIX6 to the p27Kip1 promoter in embryonal retina. SIX6 corepression seems also to involve NCOR1, TBL1, HDAC1 and HDAC3. May be involved together with PAX3, SIX1, and EYA2 in regulation of myogenesis. In the developing somite, expression of DACH2 and PAX3 is regulated by the overlying ectoderm, and DACH2 and PAX3 positively regulate each other's expression. Probably binds to DNA via its DACHbox-N domain. Ref.4 Ref.5
Subunit structure	Interacts with SIX6. Interacts with EYA2 By similarity. Ref.4
Subcellular location	Nucleus Probable.
Tissue specificity	Expressed in embryo, and at lower levels in the newborn. Ref.1
Developmental stage	From 8.5 to 14.5 dpc detected in nervous tissue. In the brain detected at 8.5 dpc within the prospective hindbrain, but not within the developing forebrain or midbrain. At 9.5 dpc expressed within the ventral prosencephalon, hindbrain and forebrain. Expression within the forebrain neuroectoderm flanked the optic vesicle with rostral and caudal restrictions. In addition, dorsal and ventral expression domains were observed within the hindbrain. At 10.5 to 12.5 dpc detected in the dorsal mesencephalon, in addition to the telencephalon and hindbrain. At 14.5 dpc visible in the olfactory bulbs. Detected from 9.5 to 12.5 dpc in the dorsal neural tube with the highest expression near the hindbrain. At 9.5 and 10.5 dpc detected in cells located in the dorsal and ventral neural tube and dorsal root ganglia. Detected during the development of the optic and the auditory systems. At 10.5 dpc, a small ring of ocular staining was observed suggesting expression in the lens pit. At 10.5 dpc expressed in the developing lens epithelium and in the mesenchyme surrounding the retina. However, expression in the lens placode ectoderm was not detected, suggesting that DACH2 is activated after lens vesicle formation. Low levels of expression could also be detected in the peripheral neuroretina at 10.5 and 12.5 dpc. Detected in the otic pit at 9.5 dpc and in the otic endolymphatic duct at 10.5, 11.5 and 12.5 dpc. From 10.5 to 14.5 dpc detected in the developing fore and hind limbs. At 10.5 and 11.5 dpc observed in the anterior and posterior margins and presumptive hand plate of the limb bud. At 9.5 and 10.5 dpc expressed in the limb. At 12.5 dpc is detected in the hand plate with strong expression at the margins of the limb plate. At 14.5 dpc detected in the hand plate and lateral edges of the digits. At 8.5 dpc expression was not detected in the developing somites. In contrast, from 9.5 to 12.5 dpc, expression is detected in a repeated pattern located lateral to the neural tube and in the interlimb bud region suggesting expression in somite derivatives. At 9.5 dpc detected in the forelimb dermamyotome. Also located along the lateral portion of the trunk and within a dorsal domain within the limb bud. At 10.5 dpc expressed in lateral and limb mesoderm. From 9.5 to 10.5 dpc detected in head mesenchyme and the branchial arches. At 9.5 and 10 dpc. expressed in the head mesoderm associated with the developing eye. At 10.5 dpc this pattern appears as a ring of expression surrounding the eye. At 11.5 dpc expression is still detectable in the branchial arches with strong expression at the cranial sinus. At 14.5 dpc mammary gland primordia. Detected in the nasal openings and vibrissae. Ref.1
Domain	The DACHbox-N domain forms a structure containing a DNA binding motif similar to that of the forkhead/winged helix domain By similarity.
Sequence similarities	Belongs to the DACH/dachshund family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Domain	Coiled coil
Ligand	DNA-binding
Molecular function	Developmental protein Repressor
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	development of primary female sexual characteristics Inferred from genetic interaction PubMed 18395837. Source: MGI regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q925Q8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q925Q8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     170-182: Missing.
     619-634: GGNYYCLAMAQQLCSA → A

Isoform 3 (identifier: Q925Q8-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-174: Missing.
     449-449: E → DIQLSQHHLLNTFLHWIELAPLSKKSLHHQK
     498-498: Q → QVNNISINKIN

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 634	634	Dachshund homolog 2		PRO_0000095600
Regions
	Region	76 – 162	87	DACHbox-N
	Region	488 – 568	81	DACHbox-C
	Coiled coil	494 – 588	95	Potential
	Compositional bias	357 – 360	4	Poly-Ala
Natural variations
	Alternative sequence	1 – 174	174	Missing in isoform 3.		VSP_009495
	Alternative sequence	170 – 182	13	Missing in isoform 2.		VSP_009493
	Alternative sequence	449	1	E → DIQLSQHHLLNTFLHWIELA PLSKKSLHHQK in isoform 3.		VSP_009496
	Alternative sequence	498	1	Q → QVNNISINKIN in isoform 3.		VSP_009497
	Alternative sequence	619 – 634	16	GGNYY…QLCSA → A in isoform 2.		VSP_009494
Experimental info
	Sequence conflict	507	1	N → D in BAC28138. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 838A491FFF20C410	FASTA	634	68,588
	10 20 30 40 50 60 MAVSAPPVIS ATSSSAGVPG GLFRAEPLYS SPGEPPRLTP NMINSFMANN HNGSVLGGGI 70 80 90 100 110 120 GGGSGGSSNT NTNECRMVDM HGVKVASFLM DGQELICLPQ VFDLFLKHLV GGLHTVYTKL 130 140 150 160 170 180 KRLDISPVVC TVEQVRILRG LGAIQPGVNR CKLITRKDFE TLFTDCTNAR RKRQMTRKQA 190 200 210 220 230 240 VNSSRPGRPP KRSLGVLQDN ARLLPHAVPG LLSPGLITPT GITAAAMAEA MKLQKMKLMA 250 260 270 280 290 300 MNTLQGNGSQ NGTESEPDDL NSTTGGSESS WDKDKIQSPL AASGPQHGIA HAALAGQPGL 310 320 330 340 350 360 GGAPTLNPLQ QNHLLSNRLD LPFMMMPHPL LPVSLPPASV AMAMNQMNHL NTIANMAAAA 370 380 390 400 410 420 QIHSPLSRAG ASVIKERIPE SPSPAPSLEE SHRPGSQTSS HPSSSVSSSP SQMDHHSERM 430 440 450 460 470 480 VMMPNNREEL IVDQDNGQSI KKFQRDNKEE VPAQIPVMKS PLDKIQLAPG QALHPGFPGP 490 500 510 520 530 540 FIFADSLSSV ETLLTNIQGL LKVALDNARI QEKQIQQEKK ELRIELFRER EIRENLERQL 550 560 570 580 590 600 AVELQSRSTM QKRLKKEKKA KRKLQEALEF ESKRREQVEQ ALKQATSGDS GLRMLKDSGI 610 620 630 PDIEIENSGT PHDSAAMQGG NYYCLAMAQQ LCSA
	Isoform 2 [UniParc]. Checksum: 0561FF999603AA3E	FASTA	606	65,331
	10 20 30 40 50 60 MAVSAPPVIS ATSSSAGVPG GLFRAEPLYS SPGEPPRLTP NMINSFMANN HNGSVLGGGI 70 80 90 100 110 120 GGGSGGSSNT NTNECRMVDM HGVKVASFLM DGQELICLPQ VFDLFLKHLV GGLHTVYTKL 130 140 150 160 170 180 KRLDISPVVC TVEQVRILRG LGAIQPGVNR CKLITRKDFE TLFTDCTNAS SRPGRPPKRS 190 200 210 220 230 240 LGVLQDNARL LPHAVPGLLS PGLITPTGIT AAAMAEAMKL QKMKLMAMNT LQGNGSQNGT 250 260 270 280 290 300 ESEPDDLNST TGGSESSWDK DKIQSPLAAS GPQHGIAHAA LAGQPGLGGA PTLNPLQQNH 310 320 330 340 350 360 LLSNRLDLPF MMMPHPLLPV SLPPASVAMA MNQMNHLNTI ANMAAAAQIH SPLSRAGASV 370 380 390 400 410 420 IKERIPESPS PAPSLEESHR PGSQTSSHPS SSVSSSPSQM DHHSERMVMM PNNREELIVD 430 440 450 460 470 480 QDNGQSIKKF QRDNKEEVPA QIPVMKSPLD KIQLAPGQAL HPGFPGPFIF ADSLSSVETL 490 500 510 520 530 540 LTNIQGLLKV ALDNARIQEK QIQQEKKELR IELFREREIR ENLERQLAVE LQSRSTMQKR 550 560 570 580 590 600 LKKEKKAKRK LQEALEFESK RREQVEQALK QATSGDSGLR MLKDSGIPDI EIENSGTPHD SAAMQA
	Isoform 3 [UniParc]. Checksum: 07A15FBB5660B6B9	FASTA	500	54,698
	10 20 30 40 50 60 MTRKQAVNSS RPGRPPKRSL GVLQDNARLL PHAVPGLLSP GLITPTGITA AAMAEAMKLQ 70 80 90 100 110 120 KMKLMAMNTL QGNGSQNGTE SEPDDLNSTT GGSESSWDKD KIQSPLAASG PQHGIAHAAL 130 140 150 160 170 180 AGQPGLGGAP TLNPLQQNHL LSNRLDLPFM MMPHPLLPVS LPPASVAMAM NQMNHLNTIA 190 200 210 220 230 240 NMAAAAQIHS PLSRAGASVI KERIPESPSP APSLEESHRP GSQTSSHPSS SVSSSPSQMD 250 260 270 280 290 300 HHSERMVMMP NNREELIVDQ DNGQSIKKFQ RDNKDIQLSQ HHLLNTFLHW IELAPLSKKS 310 320 330 340 350 360 LHHQKEVPAQ IPVMKSPLDK IQLAPGQALH PGFPGPFIFA DSLSSVETLL TNIQVNNISI 370 380 390 400 410 420 NKINGLLKVA LDNARIQEKQ IQQEKKELRI ELFREREIRE NLERQLAVEL QSRSTMQKRL 430 440 450 460 470 480 KKEKKAKRKL QEALEFESKR REQVEQALKQ ATSGDSGLRM LKDSGIPDIE IENSGTPHDS 490 500 AAMQGGNYYC LAMAQQLCSA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of mouse Dach2, a homologue of Drosophila dachshund." Davis R.J., Shen W., Sandler Y.I., Heanue T.A., Mardon G. Mech. Dev. 102:169-179(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE. Strain: Swiss Webster / NIH.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Strain: C57BL/6J. Tissue: Eye.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: C57BL/6. Tissue: Brain.
[4]	"Tissue-specific regulation of retinal and pituitary precursor cell proliferation." Li X., Perissi V., Liu F., Rose D.W., Rosenfeld M.G. Science 297:1180-1183(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH SIX6.
[5]	"Pax3 and Dach2 positive regulation in the developing somite." Kardon G., Heanue T.A., Tabin C.J. Dev. Dyn. 224:350-355(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF257217 mRNA. Translation: AAK39983.1. AK033050 mRNA. Translation: BAC28138.1. BC059233 mRNA. Translation: AAH59233.1.
IPI	IPI00403444. IPI00403445. IPI00403446.
RefSeq	NP_001136042.1. NM_001142570.1. NP_291083.1. NM_033605.2.
UniGene	Mm.79760.
3D structure databases
ProteinModelPortal	Q925Q8.
SMR	Q925Q8. Positions 73-169.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q925Q8. 1 interaction.
STRING	10090.ENSMUSP00000064393.
PTM databases
PhosphoSite	Q925Q8.
Proteomic databases
PRIDE	Q925Q8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000067219; ENSMUSP00000064393; ENSMUSG00000025592. ENSMUST00000113380; ENSMUSP00000109007; ENSMUSG00000025592. ENSMUST00000113382; ENSMUSP00000109009; ENSMUSG00000025592.
GeneID	93837.
KEGG	mmu:93837.
Organism-specific databases
CTD	117154.
MGI	MGI:1890446. Dach2.
Phylogenomic databases
eggNOG	NOG279124.
GeneTree	ENSGT00390000001134.
HOGENOM	HOG000293309.
HOVERGEN	HBG065810.
InParanoid	Q925Q8.
OrthoDB	EOG4NKBVG.
Gene expression databases
ArrayExpress	Q925Q8.
Bgee	Q925Q8.
CleanEx	MM_DACH2.
Genevestigator	Q925Q8.
GermOnline	ENSMUSG00000025592. Mus musculus.
Family and domain databases
Gene3D	3.10.260.20. 1 hit.
InterPro	IPR009061. DNA-bd_dom_put. IPR003380. Transform_Ski. [Graphical view]
Pfam	PF02437. Ski_Sno. 1 hit. [Graphical view]
SUPFAM	SSF46955. Putativ_DNA_bind. 1 hit.
ProtoNet	Search...
Other
ChiTaRS	DACH2. mouse.
NextBio	351689.
SOURCE	Search...

Entry information

Entry name

DACH2_MOUSE

Accession

Primary (citable) accession number: Q925Q8
Secondary accession number(s): Q8BMA0

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2004
Last sequence update:	December 1, 2001
Last modified:	April 3, 2013
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program