Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

ATPase family AAA domain-containing protein 3

Gene

Atad3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Essential for mitochondrial network organization, mitochondrial metabolism and cell growth at organism and cellular level. May play an important role in mitochondrial protein synthesis. May also participate in mitochondrial DNA replication. May bind to mitochondrial DNA D-loops and contribute to nucleoid stability. Required for enhanced channeling of cholesterol for hormone-dependent steroidogenesis (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi351 – 358ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

Keywordsi

LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-6798695 Neutrophil degranulation

Names & Taxonomyi

Protein namesi
Recommended name:
ATPase family AAA domain-containing protein 3
Alternative name(s):
AAA-ATPase TOB3
Gene namesi
Name:Atad3
Synonyms:Atad3a, Kiaa1273
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1919214 Atad3a

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini2 – 245Mitochondrial intermembraneSequence analysisAdd BLAST244
Transmembranei246 – 263HelicalSequence analysisAdd BLAST18
Topological domaini264 – 586Mitochondrial matrixSequence analysisAdd BLAST323

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane, Mitochondrion nucleoid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000848012 – 591ATPase family AAA domain-containing protein 3Add BLAST590

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei490N6-acetyllysine; alternateCombined sources1
Modified residuei490N6-succinyllysine; alternateCombined sources1
Modified residuei494N6-acetyllysineCombined sources1
Modified residuei512N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ925I1
PaxDbiQ925I1
PeptideAtlasiQ925I1
PRIDEiQ925I1

PTM databases

iPTMnetiQ925I1
PhosphoSitePlusiQ925I1
SwissPalmiQ925I1

Expressioni

Tissue specificityi

Expressed in heart, spleen, kidney, liver and at smaller levels, in lung and muscle (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000029036
CleanExiMM_ATAD3A
ExpressionAtlasiQ925I1 baseline and differential
GenevisibleiQ925I1 MM

Interactioni

Subunit structurei

Can form homooligomers. Homodimer formation at the N-terminus may be regulated by ATP and is required for the interaction with the inner surface of the mitochondrial outer membrane and correct mitochondrial homeostasis. Interacts with components of the mitochondrial ribosome and with other proteins involved in mitochondrial RNA metabolism. May also interact with protein involved in lipid metabolism, including STARD9. May interact with FAM210A. Interacts with GADD45GIP1. Interacts with S100B in a Ca(+2)- and Zn(+2)-dependent manner; this interaction probably occurs in the cytosol prior to mitochondrial targeting. S100B could assist ATAD3A cytoplasmic processing, preventing aggregation and favoring mitochondrial localization. Interacts with HSP60/HSPD1. Forms heterooligomers with ATAD3B; this interaction may affect ATAD3A activity (By similarity).By similarity

Protein-protein interaction databases

BioGridi224456, 6 interactors
DIPiDIP-32373N
IntActiQ925I1, 7 interactors
MINTiQ925I1
STRINGi10090.ENSMUSP00000030903

Structurei

3D structure databases

ProteinModelPortaliQ925I1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 49Required for interaction with the inner surface of the mitochondrial outer membraneBy similarityAdd BLAST48
Regioni289 – 304S100B-bindingBy similarityAdd BLAST16

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili55 – 216Sequence analysisAdd BLAST162

Domaini

The transmembrane domain and a C-terminal adjacent region contain all information necessary for mitochondrial targeting.By similarity

Sequence similaritiesi

Belongs to the AAA ATPase family.Curated

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0742 Eukaryota
COG1223 LUCA
GeneTreeiENSGT00730000111059
HOGENOMiHOG000231291
HOVERGENiHBG058506
InParanoidiQ925I1
KOiK17681
OMAiAEHSGMD
OrthoDBiEOG091G04JN
PhylomeDBiQ925I1
TreeFamiTF313922

Family and domain databases

InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR003959 ATPase_AAA_core
IPR021911 DUF3523
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF00004 AAA, 1 hit
PF12037 DUF3523, 1 hit
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q925I1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSWLFGIKGP KGEGTGPPLP LPPAQPGAEG GGDRGAGDRP SPKDKWSNFD
60 70 80 90 100
PTGLERAAKA ARELEHSRHA KEALSLAQMQ EQTLQLEQQS KLKEYEAAVE
110 120 130 140 150
QLKSEQIRVQ AEERRKTLTE ETRQHQARAQ YQDKLARQRY EDQLKQQQLL
160 170 180 190 200
NEENLRKQEE SVQKQEAIRR ATVEREMELR HKNEMLRVEA EARARAKADR
210 220 230 240 250
ENADIIREQI RLKAAEHRQT ILESIRTAGT LLGEGFRAFV TDWDKVTATV
260 270 280 290 300
AGLTLLAVGV YSAKNATSVA GRYIEARLGK PSLVRETSRI SVLEALRHPI
310 320 330 340 350
QVSRRLVSRP QDALEGVILS PSLEARVRDI AIATRNTKKN KSLYRNVLMY
360 370 380 390 400
GPPGTGKTLF AKKLALHSGM DYAIMTGGDV APMGREGVTA MHKVFDWAST
410 420 430 440 450
SRRGLLLFVD EADAFLRKRA TEKISEDLRA TLNAFLHRTG QHSSKFMLVL
460 470 480 490 500
ASNQPEQFDW AINDRIDEMV CFALPQREER ERLVRMYFDK YVLKPATEGK
510 520 530 540 550
QRLKVAQFDY GKKCSEVAQL TEGMSGREIA QLAVAWQAMA YSSEDGVLTE
560 570 580 590
AMMDARVQDA VQQHQQKMQW LKVERPDSQT NKPPHPSLLS C
Length:591
Mass (Da):66,742
Last modified:December 1, 2001 - v1
Checksum:i66B599114B94620E
GO
Isoform 2 (identifier: Q925I1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     422-501: EKISEDLRAT...VLKPATEGKQ → R

Note: No experimental confirmation available.
Show »
Length:512
Mass (Da):57,428
Checksum:i8A2573513F8051BF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti376T → K in BAC36055 (PubMed:15368895).Curated1
Sequence conflicti447M → I in BAE42791 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_015643422 – 501EKISE…TEGKQ → R in isoform 2. 1 PublicationAdd BLAST80

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF343079 mRNA Translation: AAK38648.1
AK075921 mRNA Translation: BAC36055.1
AK148974 mRNA Translation: BAE28707.1
AK149702 mRNA Translation: BAE29037.1
AK150037 mRNA Translation: BAE29259.1
AK166099 mRNA Translation: BAE38570.1
AK172038 mRNA Translation: BAE42791.1
AK173141 Unassigned RNA Translation: BAD32419.1
AL670236 Genomic DNA Translation: CAM18382.1
CH466594 Genomic DNA Translation: EDL15028.1
BC023301 mRNA Translation: AAH23301.1
BC058373 mRNA Translation: AAH58373.1
BC060036 mRNA Translation: AAH60036.1
CCDSiCCDS19037.1 [Q925I1-1]
RefSeqiNP_849534.2, NM_179203.3 [Q925I1-1]
UniGeneiMm.241152

Genome annotation databases

EnsembliENSMUST00000030903; ENSMUSP00000030903; ENSMUSG00000029036 [Q925I1-1]
GeneIDi108888
KEGGimmu:108888
UCSCiuc008wen.2 mouse [Q925I1-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiATAD3_MOUSE
AccessioniPrimary (citable) accession number: Q925I1
Secondary accession number(s): A2AD89
, Q3TA78, Q3UE74, Q69ZM7, Q8C6C6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: December 1, 2001
Last modified: May 23, 2018
This is version 137 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health