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Q925H1 (TRPS1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Zinc finger transcription factor Trps1
Gene names
Name:Trps1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1281 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transcriptional repressor. Binds specifically to GATA sequences and represses expression of GATA-regulated genes at selected sites and stages in vertebrate development. Regulates chondrocyte proliferation and differentiation. Executes multiple functions in proliferating chondrocytes, expanding the region of distal chondrocytes, activating proliferation in columnar cells and supporting the differentiation of columnar into hypertrophic chondrocytes. Ref.1 Ref.3

Subunit structure

Interacts with RNF4; regulates TRPS1 repressor activity. Interacts specifically with the activator form of GLI3 (GLI3A) but not with the repressor form (GLI3R). Ref.2 Ref.3

Subcellular location

Nucleus.

Tissue specificity

In the embryo, expression is detected in both visceral and skeletal tissues. Found in the maxilla, mandible, snout, prospective phalanges and in the femoral head within the developing hip. Also expressed in the hair follicles.

Developmental stage

Detected prior to E7.5, with peak levels at around E11.5. In the developing limbs and face, levels are highest at E13.5 and decline dramatically thereafter. Ref.1

Post-translational modification

Sumoylated. Sumoylation in the repressor domain inhibits the transcription repression activity. Sumoylation on Lys-1201 is the major site. Appears to be sumoylated on multiple sites By similarity.

Sequence similarities

Contains 7 C2H2-type zinc fingers.

Contains 1 GATA-type zinc finger.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   DomainRepeat
Zinc-finger
   LigandDNA-binding
Metal-binding
Zinc
   Molecular functionRepressor
   PTMIsopeptide bond
Phosphoprotein
Ubl conjugation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processchondrocyte differentiation

Inferred from genetic interaction PubMed 18363966. Source: MGI

negative regulation of transcription from RNA polymerase II promoter

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of transcription, DNA-templated

Inferred from physical interaction PubMed 12444977. Source: MGI

positive regulation of extrinsic apoptotic signaling pathway via death domain receptors

Inferred from genetic interaction PubMed 18363966. Source: MGI

regulation of chondrocyte differentiation

Inferred from mutant phenotype Ref.3. Source: UniProtKB

regulation of histone deacetylation

Inferred from electronic annotation. Source: InterPro

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

transmembrane receptor protein serine/threonine kinase signaling pathway

Inferred from genetic interaction PubMed 18363966. Source: MGI

   Cellular_componentnucleus

Inferred from sequence or structural similarity. Source: UniProtKB

   Molecular_functionprotein binding

Inferred from physical interaction Ref.2Ref.3. Source: UniProtKB

sequence-specific DNA binding

Inferred from electronic annotation. Source: InterPro

sequence-specific DNA binding transcription factor activity

Inferred from electronic annotation. Source: InterPro

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 12811281Zinc finger transcription factor Trps1
PRO_0000083509

Regions

Zinc finger222 – 24726C2H2-type 1; atypical
Zinc finger333 – 35826C2H2-type 2; atypical
Zinc finger614 – 63724C2H2-type 3; atypical
Zinc finger666 – 68924C2H2-type 4
Zinc finger692 – 71524C2H2-type 5
Zinc finger896 – 92025GATA-type
Zinc finger1215 – 123723C2H2-type 6
Zinc finger1243 – 126725C2H2-type 7
Region635 – 819185Mediates interaction with GLI3 By similarity
Region985 – 1184200Mediates interaction with RNF4
Region1163 – 1281119Transcriptional repressor domain By similarity

Amino acid modifications

Modified residue10411Phosphoserine By similarity
Modified residue10851Phosphoserine By similarity
Cross-link1192Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) By similarity
Cross-link1201Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q925H1 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: F0A71900C2357898

FASTA1,281141,035
        10         20         30         40         50         60 
MVRKKHPPLR NVASEGEGQT LEPTATESKV SGKNKELSAD QMSENTDQSD VAELNSKEEH 

        70         80         90        100        110        120 
STHGQEPSSS GKKDLQISGL SEKAGFNYES PSKGGSLVSF PHDEVTDRNM LAFSSPAAGG 

       130        140        150        160        170        180 
VCEPLKSPQR AEADDPQDMA CTPSGDSLET KEEHKMSPKA TEETGPVQSG QANCQGLSPV 

       190        200        210        220        230        240 
SVASKNPQVP SDGGVRLSKP KGDLLVNDNP DPAPLSPELQ DFKCNICGYG YYGNDPTDLI 

       250        260        270        280        290        300 
KHFRKYHLGL HNRTRQDAEL DSKILALHNM VQFSHSKDFQ KVNRSVLSGV LQDISSSRPA 

       310        320        330        340        350        360 
LLNGTYDVQV TSGGTFIGIG RKTPDCQGNT KYFRCKFCNF TYMGNSSTEL EQHFLQTHPN 

       370        380        390        400        410        420 
KIKVSLPSSE GVKPSEKNSN KSIPALRASD SGDVGKWQDK MTVKAGDDTP VGYSVPIKPL 

       430        440        450        460        470        480 
DSSRQNGTEA TSYYWCKFCS FSCESSSSLK LLEHYGKQHG AVQSGGLNPE LNDKLPRGSV 

       490        500        510        520        530        540 
INQNDLAKSV EGEPLTKPEK GLSGAKKKDF PSKGAEDNMV TSYNCQFCDF RYSKSHGPDV 

       550        560        570        580        590        600 
IVVGPLLRHY QQLHNIHKCT IKHCPFCPRG LCSPEKHLGE ITYPFACRKS NCSHCALLLL 

       610        620        630        640        650        660 
HLSPGVAGSS RVKHQCHQCS FSTPDVDVLL FHYETVHESQ ASDVKQEANH LLGSDGQQAV 

       670        680        690        700        710        720 
RDSKEHSCTK CDFITQVEEE ISRHYRRAHS CYKCRQCSFT AADTQSLLEH FNTVHCQEQE 

       730        740        750        760        770        780 
ITTANGEEGG HAIPTIKEEP KIDLKVYSLL NPDSKMGETV PESIVKREKL DDKEGLKDKI 

       790        800        810        820        830        840 
WTESSTDDLR GVAWRGADIL RGSPSYTQAS LGLLTPVSSS QEQTKTLRDS PNVEAAHLAR 

       850        860        870        880        890        900 
PMYGLAVDTK GFLQGAPAGS EKSASLTQQY PASGESKTKD ESQSLLRRRR GSGVFCANCL 

       910        920        930        940        950        960 
TTKTSLWRKN ANGGYVCNAC GLYQKLHSTP RPLNIIKQNN GEQIIRRRTR KRLNPEALQA 

       970        980        990       1000       1010       1020 
EQLNKQQRGS GEEQVNGSPL ERRSEDHLSE SHPREIPLPS LSKYEAQGSL TKSHSAQQPV 

      1030       1040       1050       1060       1070       1080 
LVSQALDIHK RMQPLHIQIK SPQESTGDPG NSSSVSDGKG SSERGSPIEK YMRPAKHPNY 

      1090       1100       1110       1120       1130       1140 
SPPGSPIEKY QYPLFGVPFV HNDFQSEADW LRFWSKYKLS VPGNPHYLSH VPGLPNPCQN 

      1150       1160       1170       1180       1190       1200 
YVPYPTFNLP PHFSAVGSDN DIPLDLAIKH SRPGPTANGA SKEKTKAPPT VKNEDPLNVV 

      1210       1220       1230       1240       1250       1260 
KTEKVDRSTQ DELSTKCVHC GIVFLDEVMY ALHMSCHGDS GPFQCSICQH LCTDKYDFTT 

      1270       1280 
HIQRGLHRNN AQAEKNGKPK E 

« Hide

References

[1]"Transcriptional repression and developmental functions of the atypical vertebrate GATA protein TRPS1."
Malik T.H., Shoichet S.A., Latham P., Kroll T.G., Peters L.L., Shivdasani R.A.
EMBO J. 20:1715-1725(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE.
Tissue: Fetal intestine.
[2]"The RING finger protein RNF4, a co-regulator of transcription, interacts with the TRPS1 transcription factor."
Kaiser F.J., Moeroey T., Chang G.T., Horsthemke B., Luedecke H.J.
J. Biol. Chem. 278:38780-38785(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH RNF4.
[3]"Trps1, a regulator of chondrocyte proliferation and differentiation, interacts with the activator form of Gli3."
Wuelling M., Kaiser F.J., Buelens L.A., Braunholz D., Shivdasani R.A., Depping R., Vortkamp A.
Dev. Biol. 328:40-53(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH GLI3.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF346836 mRNA. Translation: AAK39508.1.
CCDSCCDS27460.1.
UniGeneMm.30466.

3D structure databases

ProteinModelPortalQ925H1.
SMRQ925H1. Positions 423-468, 523-646, 686-726, 893-938.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000077089.

PTM databases

PhosphoSiteQ925H1.

Proteomic databases

PaxDbQ925H1.
PRIDEQ925H1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

MGIMGI:1927616. Trps1.

Phylogenomic databases

eggNOGCOG5641.
HOGENOMHOG000146438.
HOVERGENHBG067120.
InParanoidQ925H1.
PhylomeDBQ925H1.

Gene expression databases

CleanExMM_TRPS1.
GenevestigatorQ925H1.

Family and domain databases

Gene3D3.30.50.10. 1 hit.
InterProIPR028440. Trps1.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR000679. Znf_GATA.
IPR013088. Znf_NHR/GATA.
[Graphical view]
PANTHERPTHR10071:SF6. PTHR10071:SF6. 1 hit.
PfamPF00320. GATA. 1 hit.
[Graphical view]
PRINTSPR00619. GATAZNFINGER.
SMARTSM00355. ZnF_C2H2. 9 hits.
SM00401. ZnF_GATA. 1 hit.
[Graphical view]
PROSITEPS00344. GATA_ZN_FINGER_1. 1 hit.
PS50114. GATA_ZN_FINGER_2. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 2 hits.
PS50157. ZINC_FINGER_C2H2_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSTRPS1. mouse.
PROQ925H1.
SOURCESearch...

Entry information

Entry nameTRPS1_MOUSE
AccessionPrimary (citable) accession number: Q925H1
Entry history
Integrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: December 1, 2001
Last modified: July 9, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot