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Protein

Acid-sensing ion channel 2

Gene

Asic2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cation channel with high affinity for sodium, which is gated by extracellular protons and inhibited by the diuretic amiloride. Also permeable for Li+ and K+. Generates a biphasic current with a fast inactivating and a slow sustained phase. Heteromeric channel assembly seems to modulate.1 Publication

GO - Molecular functioni

  • cation channel activity Source: MGI
  • ion channel activity Source: MGI
  • ion gated channel activity Source: MGI
  • ligand-gated sodium channel activity Source: MGI

GO - Biological processi

  • cation transmembrane transport Source: GOC
  • cation transport Source: MGI
  • detection of mechanical stimulus involved in sensory perception Source: MGI
  • ion transmembrane transport Source: MGI
  • negative regulation of apoptotic process Source: MGI
  • phototransduction Source: MGI
  • positive regulation of synapse assembly Source: MGI
  • protein localization to synapse Source: MGI
  • regulation of blood coagulation Source: MGI
  • regulation of gene expression Source: MGI
  • regulation of ion transmembrane transport Source: MGI
  • regulation of membrane potential Source: MGI
  • regulation of systemic arterial blood pressure by aortic arch baroreceptor feedback Source: MGI
  • regulation of vasoconstriction Source: MGI
  • response to acidic pH Source: MGI
  • response to mechanical stimulus Source: MGI
  • sensory perception of sound Source: MGI
  • sensory perception of sour taste Source: MGI
  • sodium ion transmembrane transport Source: GOC
  • sodium ion transport Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Sodium channel

Keywords - Biological processi

Ion transport, Sodium transport, Transport

Keywords - Ligandi

Sodium

Enzyme and pathway databases

ReactomeiR-MMU-2672351. Stimuli-sensing channels.

Protein family/group databases

TCDBi1.A.6.1.8. the epithelial na(+) channel (enac) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Acid-sensing ion channel 2
Short name:
ASIC2
Alternative name(s):
Amiloride-sensitive brain sodium channel
Amiloride-sensitive cation channel 1, neuronal
Brain sodium channel 1
Short name:
BNC1
Short name:
BNaC1
Gene namesi
Name:Asic2
Synonyms:Accn1, Bnac1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1100867. Asic2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3737CytoplasmicBy similarityAdd
BLAST
Transmembranei38 – 5821HelicalSequence analysisAdd
BLAST
Topological domaini59 – 427369ExtracellularBy similarityAdd
BLAST
Transmembranei428 – 44821HelicalSequence analysisAdd
BLAST
Topological domaini449 – 51264CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

  • dendritic spine Source: MGI
  • integral component of membrane Source: MGI
  • integral component of plasma membrane Source: MGI
  • neuronal cell body Source: MGI
  • neuron projection Source: MGI
  • synapse Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice display altered rod phototransduction and neurotransmission, associated with increased light-induced retina damages.1 Publication

Chemistry

ChEMBLiCHEMBL3232695.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 512512Acid-sensing ion channel 2PRO_0000181291Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei8 – 81PhosphoserineBy similarity
Modified residuei11 – 111PhosphoserineBy similarity
Disulfide bondi92 ↔ 193By similarity
Disulfide bondi171 ↔ 178By similarity
Disulfide bondi289 ↔ 364By similarity
Disulfide bondi307 ↔ 360By similarity
Disulfide bondi311 ↔ 358By similarity
Disulfide bondi320 ↔ 342By similarity
Disulfide bondi322 ↔ 334By similarity
Glycosylationi365 – 3651N-linked (GlcNAc...)By similarity
Glycosylationi392 – 3921N-linked (GlcNAc...)By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ925H0.
PRIDEiQ925H0.

PTM databases

PhosphoSiteiQ925H0.

Expressioni

Tissue specificityi

Expressed by sensory neurons. Expressed by nociceptive sensory neurons, spiral ganglion (SG) neurons and the retina (at protein level). Isoform 1 and isoform 2 are expressed in outer nuclear layer of retina (photoreceptors) and to a lower extent in distal and proximal inner nuclear layer.2 Publications

Developmental stagei

Expression changes dramatically during cochlear development. Expression is detected at E11.5 in otocyst and increases in the SG neurons after E18.5. Also detected in the lumen side of all cells forming the vestibular cavity and at the top of the macula of saccule and utricle. Before birth expressed by epithelial cells facing the endolymphatic space. Post-natally expressed by cells in the apical turn of the cochlea, while expression on the lumen side of the membranous labyrinth decreases. Expression shifts gradually toward the top of supporting cells and the spiral limbus. Also expressed by vestibular ganglion neurons. Restricted to the SG neurons in the mature cochlea (at protein level).1 Publication

Inductioni

Expression in a subset of neurons may be regulated by neurotrophins.1 Publication

Gene expression databases

BgeeiQ925H0.
ExpressionAtlasiQ925H0. baseline and differential.
GenevisibleiQ925H0. MM.

Interactioni

Subunit structurei

Homotrimer or heterotrimer with other ASIC proteins (By similarity). Interacts with PRKCABP and ASIC3 (By similarity). Interacts with STOM; this regulates channel activity.By similarity2 Publications

Protein-protein interaction databases

BioGridi197917. 2 interactions.
MINTiMINT-223604.

Structurei

3D structure databases

ProteinModelPortaliQ925H0.
SMRiQ925H0. Positions 50-450.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00760000119120.
HOGENOMiHOG000247010.
HOVERGENiHBG004150.
InParanoidiQ925H0.
KOiK04828.
OMAiCESKALG.
OrthoDBiEOG72VH5P.
TreeFamiTF330663.

Family and domain databases

InterProiIPR004724. ENaC.
IPR001873. Na+channel_ASC.
IPR020903. Na+channel_ASC_CS.
[Graphical view]
PANTHERiPTHR11690. PTHR11690. 1 hit.
PfamiPF00858. ASC. 1 hit.
[Graphical view]
PRINTSiPR01078. AMINACHANNEL.
TIGRFAMsiTIGR00859. ENaC. 1 hit.
PROSITEiPS01206. ASC. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q925H0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDLKESPSEG SLQPSSIQIF ANTSTLHGIR HIFVYGPLTI RRVLWAVAFV
60 70 80 90 100
GSLGLLLVES SERVSYYFSY QHVTKVDEVV AQSLVFPAVT LCNLNGFRFS
110 120 130 140 150
RLTTNDLYHA GELLALLDVN LQIPDPHLAD PTVLEALRQK ANFKHYKPKQ
160 170 180 190 200
FSMLEFLHRV GHDLKDMMLY CKFKGQECGH QDFTTVFTKY GKCYMFNSGE
210 220 230 240 250
DGKPLLTTVK GGTGNGLEIM LDIQQDEYLP IWGETEETTF EAGVKVQIHS
260 270 280 290 300
QSEPPFIQEL GFGVAPGFQT FVATQEQRLT YLPPPWGECR SSEMGLDFFP
310 320 330 340 350
VYSITACRID CETRYIVENC NCRMVHMPGD APFCTPEQHK ECAEPALGLL
360 370 380 390 400
AEKDSNYCLC RTPCNLTRYN KELSMVKIPS KTSAKYLEKK FNKSEKYISE
410 420 430 440 450
NILVLDIFFE ALNYETIEQK KAYEVAALLG DIGGQMGLFI GASILTILEL
460 470 480 490 500
FDYIYELIKE KLLDLLGKEE EEGSHDENMS TCDTMPNHSE TISHTVNVPL
510
QTALGTLEEI AC
Length:512
Mass (Da):57,739
Last modified:December 1, 2001 - v1
Checksum:i7D81A77C3B347B04
GO
Isoform 2 (identifier: Q925H0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-184: Missing.
     185-185: T → MSRSGGARLP...ELCGPHNFSS

Show »
Length:563
Mass (Da):63,204
Checksum:i56F7B4A0A214BA39
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 184184Missing in isoform 2. 1 PublicationVSP_015592Add
BLAST
Alternative sequencei185 – 1851T → MSRSGGARLPATALSGPGRF RMAREQPAPAAVAAARQPGG DRSGDRELQGPGVARRGRPS LSRTKLHGLRHMCAGRTAAG GSFQRRALWVLAFCTSLGLL LSWSSNRLLYWLSFPSHTRV HREWSRQLPFPAVTVCNNNP LRFPRLSKGDLYYAGHWLGL LLPNRTARPLVSELLRGDEP RRQWFRKLADFRLFLPPRHF EGISAAFMDRLGHQLEDMLL SCKYRGELCGPHNFSS in isoform 2. 1 PublicationVSP_015593

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y14634 mRNA. Translation: CAA74978.1.
AF348465 mRNA. Translation: AAK40101.1.
AL645842, AL626807, AL663025 Genomic DNA. Translation: CAI24499.1.
AL645842, AL645626, AL663025 Genomic DNA. Translation: CAI24500.1.
AL626807, AL645842, AL663025 Genomic DNA. Translation: CAI25306.1.
AL645626, AL645842, AL663025 Genomic DNA. Translation: CAI25540.1.
AL663025, AL626807, AL645842 Genomic DNA. Translation: CAI25600.1.
AL663025, AL645626, AL645842 Genomic DNA. Translation: CAI25602.1.
CCDSiCCDS25137.1. [Q925H0-2]
CCDS36243.1. [Q925H0-1]
RefSeqiNP_001029185.1. NM_001034013.2. [Q925H0-1]
NP_031410.1. NM_007384.3. [Q925H0-2]
UniGeneiMm.234998.
Mm.259072.
Mm.330264.
Mm.366419.
Mm.473675.

Genome annotation databases

EnsembliENSMUST00000021045; ENSMUSP00000021045; ENSMUSG00000020704. [Q925H0-2]
ENSMUST00000066197; ENSMUSP00000067095; ENSMUSG00000020704. [Q925H0-1]
GeneIDi11418.
KEGGimmu:11418.
UCSCiuc007kmm.2. mouse. [Q925H0-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y14634 mRNA. Translation: CAA74978.1.
AF348465 mRNA. Translation: AAK40101.1.
AL645842, AL626807, AL663025 Genomic DNA. Translation: CAI24499.1.
AL645842, AL645626, AL663025 Genomic DNA. Translation: CAI24500.1.
AL626807, AL645842, AL663025 Genomic DNA. Translation: CAI25306.1.
AL645626, AL645842, AL663025 Genomic DNA. Translation: CAI25540.1.
AL663025, AL626807, AL645842 Genomic DNA. Translation: CAI25600.1.
AL663025, AL645626, AL645842 Genomic DNA. Translation: CAI25602.1.
CCDSiCCDS25137.1. [Q925H0-2]
CCDS36243.1. [Q925H0-1]
RefSeqiNP_001029185.1. NM_001034013.2. [Q925H0-1]
NP_031410.1. NM_007384.3. [Q925H0-2]
UniGeneiMm.234998.
Mm.259072.
Mm.330264.
Mm.366419.
Mm.473675.

3D structure databases

ProteinModelPortaliQ925H0.
SMRiQ925H0. Positions 50-450.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi197917. 2 interactions.
MINTiMINT-223604.

Chemistry

ChEMBLiCHEMBL3232695.

Protein family/group databases

TCDBi1.A.6.1.8. the epithelial na(+) channel (enac) family.

PTM databases

PhosphoSiteiQ925H0.

Proteomic databases

MaxQBiQ925H0.
PRIDEiQ925H0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000021045; ENSMUSP00000021045; ENSMUSG00000020704. [Q925H0-2]
ENSMUST00000066197; ENSMUSP00000067095; ENSMUSG00000020704. [Q925H0-1]
GeneIDi11418.
KEGGimmu:11418.
UCSCiuc007kmm.2. mouse. [Q925H0-1]

Organism-specific databases

CTDi40.
MGIiMGI:1100867. Asic2.

Phylogenomic databases

GeneTreeiENSGT00760000119120.
HOGENOMiHOG000247010.
HOVERGENiHBG004150.
InParanoidiQ925H0.
KOiK04828.
OMAiCESKALG.
OrthoDBiEOG72VH5P.
TreeFamiTF330663.

Enzyme and pathway databases

ReactomeiR-MMU-2672351. Stimuli-sensing channels.

Miscellaneous databases

NextBioi278658.
PROiQ925H0.
SOURCEiSearch...

Gene expression databases

BgeeiQ925H0.
ExpressionAtlasiQ925H0. baseline and differential.
GenevisibleiQ925H0. MM.

Family and domain databases

InterProiIPR004724. ENaC.
IPR001873. Na+channel_ASC.
IPR020903. Na+channel_ASC_CS.
[Graphical view]
PANTHERiPTHR11690. PTHR11690. 1 hit.
PfamiPF00858. ASC. 1 hit.
[Graphical view]
PRINTSiPR01078. AMINACHANNEL.
TIGRFAMsiTIGR00859. ENaC. 1 hit.
PROSITEiPS01206. ASC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A modulatory subunit of acid sensing ion channels in brain and dorsal root ganglion cells."
    Lingueglia E., de Weille J.R., Bassilana F., Heurteaux C., Sakai H., Waldmann R., Lazdunski M.
    J. Biol. Chem. 272:29778-29783(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    Strain: BALB/cJ.
    Tissue: Brain.
  2. "Transport and localization of the DEG/ENaC ion channel BNaC1alpha to peripheral mechanosensory terminals of dorsal root ganglia neurons."
    Garcia-Anoveros J., Samad T.A., Zuvela-Jelaska L., Woolf C.J., Corey D.P.
    J. Neurosci. 21:2678-2686(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Strain: BALB/cJ.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. Cited for: INTERACTION WITH STOM.
  5. "Acid-sensing ion channel 2 is important for retinal function and protects against light-induced retinal degeneration."
    Ettaiche M., Guy N., Hofman P., Lazdunski M., Waldmann R.
    J. Neurosci. 24:1005-1012(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, FUNCTION, DISRUPTION PHENOTYPE.
  6. "Acid-sensing ion channel 2 contributes a major component to acid-evoked excitatory responses in spiral ganglion neurons and plays a role in noise susceptibility of mice."
    Peng B.-G., Ahmad S., Chen S., Chen P., Price M.P., Lin X.
    J. Neurosci. 24:10167-10175(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.
  7. "The sensory mechanotransduction ion channel ASIC2 (acid sensitive ion channel 2) is regulated by neurotrophin availability."
    McIlwrath S.L., Hu J., Anirudhan G., Shin J.-B., Lewin G.R.
    Neuroscience 131:499-511(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, INDUCTION BY NEUROTROPHIN.
  8. Cited for: INTERACTION WITH STOM.

Entry informationi

Entry nameiASIC2_MOUSE
AccessioniPrimary (citable) accession number: Q925H0
Secondary accession number(s): Q5SUU2, Q61203
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: December 1, 2001
Last modified: May 11, 2016
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Regulated by Zn2+. Inhibited by anti-inflammatory drugs like salicylic acid (By similarity).By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.