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Protein

Endothelial cell-selective adhesion molecule

Gene

Esam

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Can mediate aggregation most likely through a homophilic molecular interaction.2 Publications

GO - Biological processi

  • calcium-independent cell-cell adhesion via plasma membrane cell-adhesion molecules Source: MGI
  • homophilic cell adhesion via plasma membrane adhesion molecules Source: MGI
  • single organismal cell-cell adhesion Source: MGI
Complete GO annotation...

Keywords - Biological processi

Cell adhesion

Enzyme and pathway databases

ReactomeiR-MMU-202733. Cell surface interactions at the vascular wall.

Names & Taxonomyi

Protein namesi
Recommended name:
Endothelial cell-selective adhesion molecule
Gene namesi
Name:Esam
Synonyms:Esam1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1916774. Esam.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini30 – 251ExtracellularSequence analysisAdd BLAST222
Transmembranei252 – 272HelicalSequence analysisAdd BLAST21
Topological domaini273 – 394CytoplasmicSequence analysisAdd BLAST122

GO - Cellular componenti

  • adherens junction Source: MGI
  • bicellular tight junction Source: MGI
  • extracellular exosome Source: MGI
  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Tight junction

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 29By similarityAdd BLAST29
ChainiPRO_000001475430 – 394Endothelial cell-selective adhesion moleculeAdd BLAST365

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi111N-linked (GlcNAc...)Sequence analysis1
Glycosylationi172N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi177 ↔ 227PROSITE-ProRule annotation
Glycosylationi216N-linked (GlcNAc...)Sequence analysis1
Glycosylationi239N-linked (GlcNAc...)Sequence analysis1
Modified residuei304PhosphoserineCombined sources1
Modified residuei336PhosphothreonineCombined sources1
Modified residuei338PhosphothreonineCombined sources1
Modified residuei340PhosphoserineCombined sources1
Modified residuei343PhosphoserineCombined sources1
Modified residuei348PhosphoserineCombined sources1
Modified residuei375PhosphoserineCombined sources1

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ925F2.
PaxDbiQ925F2.
PRIDEiQ925F2.

PTM databases

iPTMnetiQ925F2.
PhosphoSitePlusiQ925F2.

Expressioni

Tissue specificityi

Highly expressed in the heart and lung. Weakly expressed in the kidney and skin. Expression is restricted to the vascular endothelial cells. Expressed in the kidney, heart and tongue (at protein level). Also expressed on megakaryocytes and activated platelets.2 Publications

Developmental stagei

Expression in E8.5-E9.5 embryos is observed in embryonic blood vessels including the dorsal aorta, intersomitic arteries and the forming vascular plexus in the head as well as the cardiac outflow tract. By E10.5-E11.5 embryos expression is found in association with the all recognizable blood vessels and in association with cells lining the heart chambers. At 10.5-days embryos expression is associated with syncytiotrophoblasts and cytotrophoblasts as well as endothelial cells associated with blood vessels in the decidua. Expression decreased after mid-gestation from 15.5-day embryos.1 Publication

Inductioni

Up-regulated on the surface of platelets upon activation.1 Publication

Gene expression databases

BgeeiENSMUSG00000001946.
ExpressionAtlasiQ925F2. baseline and differential.
GenevisibleiQ925F2. MM.

Interactioni

Subunit structurei

Interacts with BAIAP1.1 Publication

Protein-protein interaction databases

IntActiQ925F2. 1 interactor.
MINTiMINT-4123160.
STRINGi10090.ENSMUSP00000002011.

Structurei

3D structure databases

ProteinModelPortaliQ925F2.
SMRiQ925F2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini37 – 146Ig-like V-typeAdd BLAST110
Domaini159 – 243Ig-like C2-typeAdd BLAST85

Sequence similaritiesi

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IHVB. Eukaryota.
ENOG4111DT8. LUCA.
GeneTreeiENSGT00760000119145.
HOGENOMiHOG000111222.
HOVERGENiHBG000420.
InParanoidiQ925F2.
KOiK06787.
OMAiYSMPSRN.
OrthoDBiEOG091G05PI.
PhylomeDBiQ925F2.
TreeFamiTF330875.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR013106. Ig_V-set.
[Graphical view]
PfamiPF07686. V-set. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 2 hits.
SM00408. IGc2. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 2 hits.
PROSITEiPS50835. IG_LIKE. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q925F2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MILQAGTPET SLLRVLFLGL STLAAFSRAQ MELHVPPGLN KLEAVEGEEV
60 70 80 90 100
VLPAWYTMAR EESWSHPREV PILIWFLEQE GKEPNQVLSY INGVMTNKPG
110 120 130 140 150
TALVHSISSR NVSLRLGALQ EGDSGTYRCS VNVQNDEGKS IGHSIKSIEL
160 170 180 190 200
KVLVPPAPPS CSLQGVPYVG TNVTLNCKSP RSKPTAQYQW ERLAPSSQVF
210 220 230 240 250
FGPALDAVRG SLKLTNLSIA MSGVYVCKAQ NRVGFAKCNV TLDVMTGSKA
260 270 280 290 300
AVVAGAVVGT FVGLVLIAGL VLLYQRRSKT LEELANDIKE DAIAPRTLPW
310 320 330 340 350
TKGSDTISKN GTLSSVTSAR ALRPPKAAPP RPGTFTPTPS VSSQALSSPR
360 370 380 390
LPRVDEPPPQ AVSLTPGGVS SSALSRMGAV PVMVPAQSQA GSLV
Length:394
Mass (Da):41,810
Last modified:December 1, 2001 - v1
Checksum:i3D2B354943A2227D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF361882 mRNA. Translation: AAK51504.1.
BC049249 mRNA. Translation: AAH49249.1.
CCDSiCCDS22979.1.
RefSeqiNP_081378.1. NM_027102.3.
UniGeneiMm.41751.

Genome annotation databases

EnsembliENSMUST00000002011; ENSMUSP00000002011; ENSMUSG00000001946.
GeneIDi69524.
KEGGimmu:69524.
UCSCiuc009ouz.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF361882 mRNA. Translation: AAK51504.1.
BC049249 mRNA. Translation: AAH49249.1.
CCDSiCCDS22979.1.
RefSeqiNP_081378.1. NM_027102.3.
UniGeneiMm.41751.

3D structure databases

ProteinModelPortaliQ925F2.
SMRiQ925F2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ925F2. 1 interactor.
MINTiMINT-4123160.
STRINGi10090.ENSMUSP00000002011.

PTM databases

iPTMnetiQ925F2.
PhosphoSitePlusiQ925F2.

Proteomic databases

MaxQBiQ925F2.
PaxDbiQ925F2.
PRIDEiQ925F2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000002011; ENSMUSP00000002011; ENSMUSG00000001946.
GeneIDi69524.
KEGGimmu:69524.
UCSCiuc009ouz.2. mouse.

Organism-specific databases

CTDi90952.
MGIiMGI:1916774. Esam.

Phylogenomic databases

eggNOGiENOG410IHVB. Eukaryota.
ENOG4111DT8. LUCA.
GeneTreeiENSGT00760000119145.
HOGENOMiHOG000111222.
HOVERGENiHBG000420.
InParanoidiQ925F2.
KOiK06787.
OMAiYSMPSRN.
OrthoDBiEOG091G05PI.
PhylomeDBiQ925F2.
TreeFamiTF330875.

Enzyme and pathway databases

ReactomeiR-MMU-202733. Cell surface interactions at the vascular wall.

Miscellaneous databases

PROiQ925F2.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000001946.
ExpressionAtlasiQ925F2. baseline and differential.
GenevisibleiQ925F2. MM.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR013106. Ig_V-set.
[Graphical view]
PfamiPF07686. V-set. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 2 hits.
SM00408. IGc2. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 2 hits.
PROSITEiPS50835. IG_LIKE. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiESAM_MOUSE
AccessioniPrimary (citable) accession number: Q925F2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: December 1, 2001
Last modified: November 2, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.