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Protein

Taste receptor type 1 member 3

Gene

Tas1r3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Putative taste receptor. TAS1R1/TAS1R3 responds to the umami taste stimulus (the taste of monosodium glutamate) and also to most of the 20 standard L-amino acids, but not to their D-enantiomers or other compounds. TAS1R2/TAS1R3 recognizes diverse natural and synthetic sweeteners. TAS1R3 is essential for the recognition and response to the disaccharide trehalose. Sequence differences within and between species can significantly influence the selectivity and specificity of taste responses.2 Publications

GO - Molecular functioni

  • G-protein coupled receptor activity Source: MGI
  • sweet taste receptor activity Source: Ensembl
  • taste receptor activity Source: MGI

GO - Biological processi

  • detection of chemical stimulus involved in sensory perception of sweet taste Source: MGI
  • G-protein coupled receptor signaling pathway Source: MGI
  • sensory perception of sweet taste Source: MGI
  • sensory perception of umami taste Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Keywords - Biological processi

Sensory transduction, Taste

Enzyme and pathway databases

ReactomeiR-MMU-420499. Class C/3 (Metabotropic glutamate/pheromone receptors).

Names & Taxonomyi

Protein namesi
Recommended name:
Taste receptor type 1 member 3
Alternative name(s):
Saccharin preference protein
Sweet taste receptor T1R3
Gene namesi
Name:Tas1r3
Synonyms:Sac, T1r3, Tr3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1933547. Tas1r3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini21 – 572552ExtracellularSequence analysisAdd
BLAST
Transmembranei573 – 59321Helical; Name=1Sequence analysisAdd
BLAST
Topological domaini594 – 61017CytoplasmicSequence analysisAdd
BLAST
Transmembranei611 – 63121Helical; Name=2Sequence analysisAdd
BLAST
Topological domaini632 – 64413ExtracellularSequence analysisAdd
BLAST
Transmembranei645 – 66521Helical; Name=3Sequence analysisAdd
BLAST
Topological domaini666 – 68722CytoplasmicSequence analysisAdd
BLAST
Transmembranei688 – 70821Helical; Name=4Sequence analysisAdd
BLAST
Topological domaini709 – 73527ExtracellularSequence analysisAdd
BLAST
Transmembranei736 – 75621Helical; Name=5Sequence analysisAdd
BLAST
Topological domaini757 – 76711CytoplasmicSequence analysisAdd
BLAST
Transmembranei768 – 78821Helical; Name=6Sequence analysisAdd
BLAST
Topological domaini789 – 7968ExtracellularSequence analysis
Transmembranei797 – 81721Helical; Name=7Sequence analysisAdd
BLAST
Topological domaini818 – 85841CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of plasma membrane Source: MGI
  • sweet taste receptor complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence analysisAdd
BLAST
Chaini21 – 858838Taste receptor type 1 member 3PRO_0000012962Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi58 – 581N-linked (GlcNAc...); when associated with variant T-60Sequence analysis
Glycosylationi85 – 851N-linked (GlcNAc...)Sequence analysis
Glycosylationi130 – 1301N-linked (GlcNAc...)Sequence analysis
Glycosylationi203 – 2031N-linked (GlcNAc...)Sequence analysis
Glycosylationi264 – 2641N-linked (GlcNAc...)Sequence analysis
Glycosylationi379 – 3791N-linked (GlcNAc...)Sequence analysis
Glycosylationi387 – 3871N-linked (GlcNAc...)Sequence analysis
Glycosylationi418 – 4181N-linked (GlcNAc...)Sequence analysis
Glycosylationi439 – 4391N-linked (GlcNAc...)Sequence analysis
Glycosylationi482 – 4821N-linked (GlcNAc...)Sequence analysis

Post-translational modificationi

The Thr-60 variant is predicted to introduce a novel N-linked glycosylation site at Asn-58. The addition of even a short carbohydrate group at Asn-58 is predicted to disrupt one of the contact surfaces required for stability of a dimer. Therefore a Thr-60 variant N-glycosylated at Asn-58 is predicted to be precluded from forming homodimers or heterodimers.

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ925D8.
PRIDEiQ925D8.

Expressioni

Tissue specificityi

Expressed in circumvallate, foliate and fungiform taste papillae as well as in taste buds on the palate. Also expressed in testis. Not expressed in brain, heart, kidney, liver or spleen. The topographic distribution in various taste papillae is different from those of other T1R members.4 Publications

Gene expression databases

BgeeiENSMUSG00000029072.
CleanExiMM_TAS1R3.
GenevisibleiQ925D8. MM.

Interactioni

Subunit structurei

Forms homodimers or heterodimers with TAS1R1 and TAS1R2.

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000030949.

Structurei

3D structure databases

ProteinModelPortaliQ925D8.
SMRiQ925D8. Positions 25-559.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1056. Eukaryota.
ENOG410XR6W. LUCA.
GeneTreeiENSGT00760000118884.
HOGENOMiHOG000231293.
HOVERGENiHBG098572.
InParanoidiQ925D8.
KOiK04626.
OMAiCFGLACL.
OrthoDBiEOG091G01UW.
PhylomeDBiQ925D8.
TreeFamiTF331269.

Family and domain databases

InterProiIPR001828. ANF_lig-bd_rcpt.
IPR000337. GPCR_3.
IPR011500. GPCR_3_9-Cys_dom.
IPR017978. GPCR_3_C.
IPR017979. GPCR_3_CS.
IPR028082. Peripla_BP_I.
[Graphical view]
PfamiPF00003. 7tm_3. 1 hit.
PF01094. ANF_receptor. 1 hit.
PF07562. NCD3G. 1 hit.
[Graphical view]
PRINTSiPR00248. GPCRMGR.
SUPFAMiSSF53822. SSF53822. 1 hit.
PROSITEiPS00980. G_PROTEIN_RECEP_F3_2. 1 hit.
PS50259. G_PROTEIN_RECEP_F3_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q925D8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPALAIMGLS LAAFLELGMG ASLCLSQQFK AQGDYILGGL FPLGSTEEAT
60 70 80 90 100
LNQRTQPNSI PCNRFSPLGL FLAMAMKMAV EEINNGSALL PGLRLGYDLF
110 120 130 140 150
DTCSEPVVTM KSSLMFLAKV GSQSIAAYCN YTQYQPRVLA VIGPHSSELA
160 170 180 190 200
LITGKFFSFF LMPQVSYSAS MDRLSDRETF PSFFRTVPSD RVQLQAVVTL
210 220 230 240 250
LQNFSWNWVA ALGSDDDYGR EGLSIFSSLA NARGICIAHE GLVPQHDTSG
260 270 280 290 300
QQLGKVLDVL RQVNQSKVQV VVLFASARAV YSLFSYSIHH GLSPKVWVAS
310 320 330 340 350
ESWLTSDLVM TLPNIARVGT VLGFLQRGAL LPEFSHYVET HLALAADPAF
360 370 380 390 400
CASLNAELDL EEHVMGQRCP RCDDIMLQNL SSGLLQNLSA GQLHHQIFAT
410 420 430 440 450
YAAVYSVAQA LHNTLQCNVS HCHVSEHVLP WQLLENMYNM SFHARDLTLQ
460 470 480 490 500
FDAEGNVDME YDLKMWVWQS PTPVLHTVGT FNGTLQLQQS KMYWPGNQVP
510 520 530 540 550
VSQCSRQCKD GQVRRVKGFH SCCYDCVDCK AGSYRKHPDD FTCTPCNQDQ
560 570 580 590 600
WSPEKSTACL PRRPKFLAWG EPVVLSLLLL LCLVLGLALA ALGLSVHHWD
610 620 630 640 650
SPLVQASGGS QFCFGLICLG LFCLSVLLFP GRPSSASCLA QQPMAHLPLT
660 670 680 690 700
GCLSTLFLQA AETFVESELP LSWANWLCSY LRGLWAWLVV LLATFVEAAL
710 720 730 740 750
CAWYLIAFPP EVVTDWSVLP TEVLEHCHVR SWVSLGLVHI TNAMLAFLCF
760 770 780 790 800
LGTFLVQSQP GRYNRARGLT FAMLAYFITW VSFVPLLANV QVAYQPAVQM
810 820 830 840 850
GAILVCALGI LVTFHLPKCY VLLWLPKLNT QEFFLGRNAK KAADENSGGG

EAAQGHNE
Length:858
Mass (Da):94,561
Last modified:January 4, 2005 - v2
Checksum:i4E4168279DB478F8
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti184 – 1841F → L in AAK55537 (PubMed:11326277).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti55 – 551T → A in strain: 129/J, 129/SvEv, AKR/J, BALB/c, C3H/HeJ, DBA/2J and DBA/2J. 8 Publications
Natural varianti60 – 601I → T in strain: 129/J, 129/SvEv, AKR/J, BALB/c, C3H/HeJ, DBA/2J and DBA/2J; may influence the ability to form dimers or bind sweeteners. 8 Publications
Natural varianti61 – 611P → L in strain: 129/J, 129/SvEv, AKR/J, BALB/c, C3H/HeJ, CAST/Ei, DBA/2J, FVB/N, ST/bJ and SWR/J. 7 Publications
Natural varianti261 – 2611R → C in strain: FVB/N, ST/bJ, SWR/J. 4 Publications
Natural varianti371 – 3711R → Q in strain: 129/J, 129/SvEv, AKR/J, BALB/c, CAST/Ei, C3H/HeJ, DBA/2J, FVB/N, ST/bJ and SWR/J. 6 Publications
Natural varianti692 – 6921L → S in strain: FVB/N, ST/bJ and SWR/J. 4 Publications
Natural varianti706 – 7061I → N in strain: SWR/J. 2 Publications
Natural varianti706 – 7061I → T in strain: 129/J, 129/SvEv, AKR/J, BALB/c, C3H/HeJ, DBA/2J, DBA/2J, FVB/N, ST/bJ and SWR/J. 6 Publications
Natural varianti855 – 8551G → E in strain: 129/J, AKR/J, CAST/Ei, DBA/2J and SWR/J. 4 Publications

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB049994 mRNA. Translation: BAB47181.1.
AB055708 Genomic DNA. Translation: BAB62852.1.
AF337039 mRNA. Translation: AAK39436.1.
AF311386 mRNA. Translation: AAL08425.1.
AY032621 mRNA. Translation: AAK51602.1.
AY026318 Genomic DNA. Translation: AAK01937.1.
AF368024 Genomic DNA. Translation: AAK55536.1.
AF368025 Genomic DNA. Translation: AAK55537.1.
AL670236 Genomic DNA. Translation: CAM18393.1.
CCDSiCCDS19046.1.
PIRiJC7683.
RefSeqiNP_114078.1. NM_031872.2.
UniGeneiMm.358792.

Genome annotation databases

EnsembliENSMUST00000030949; ENSMUSP00000030949; ENSMUSG00000029072.
GeneIDi83771.
KEGGimmu:83771.
UCSCiuc008wff.1. mouse.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB049994 mRNA. Translation: BAB47181.1.
AB055708 Genomic DNA. Translation: BAB62852.1.
AF337039 mRNA. Translation: AAK39436.1.
AF311386 mRNA. Translation: AAL08425.1.
AY032621 mRNA. Translation: AAK51602.1.
AY026318 Genomic DNA. Translation: AAK01937.1.
AF368024 Genomic DNA. Translation: AAK55536.1.
AF368025 Genomic DNA. Translation: AAK55537.1.
AL670236 Genomic DNA. Translation: CAM18393.1.
CCDSiCCDS19046.1.
PIRiJC7683.
RefSeqiNP_114078.1. NM_031872.2.
UniGeneiMm.358792.

3D structure databases

ProteinModelPortaliQ925D8.
SMRiQ925D8. Positions 25-559.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000030949.

Protein family/group databases

GPCRDBiSearch...

Proteomic databases

PaxDbiQ925D8.
PRIDEiQ925D8.

Protocols and materials databases

DNASUi83771.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000030949; ENSMUSP00000030949; ENSMUSG00000029072.
GeneIDi83771.
KEGGimmu:83771.
UCSCiuc008wff.1. mouse.

Organism-specific databases

CTDi83756.
MGIiMGI:1933547. Tas1r3.

Phylogenomic databases

eggNOGiKOG1056. Eukaryota.
ENOG410XR6W. LUCA.
GeneTreeiENSGT00760000118884.
HOGENOMiHOG000231293.
HOVERGENiHBG098572.
InParanoidiQ925D8.
KOiK04626.
OMAiCFGLACL.
OrthoDBiEOG091G01UW.
PhylomeDBiQ925D8.
TreeFamiTF331269.

Enzyme and pathway databases

ReactomeiR-MMU-420499. Class C/3 (Metabotropic glutamate/pheromone receptors).

Miscellaneous databases

PROiQ925D8.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000029072.
CleanExiMM_TAS1R3.
GenevisibleiQ925D8. MM.

Family and domain databases

InterProiIPR001828. ANF_lig-bd_rcpt.
IPR000337. GPCR_3.
IPR011500. GPCR_3_9-Cys_dom.
IPR017978. GPCR_3_C.
IPR017979. GPCR_3_CS.
IPR028082. Peripla_BP_I.
[Graphical view]
PfamiPF00003. 7tm_3. 1 hit.
PF01094. ANF_receptor. 1 hit.
PF07562. NCD3G. 1 hit.
[Graphical view]
PRINTSiPR00248. GPCRMGR.
SUPFAMiSSF53822. SSF53822. 1 hit.
PROSITEiPS00980. G_PROTEIN_RECEP_F3_2. 1 hit.
PS50259. G_PROTEIN_RECEP_F3_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTS1R3_MOUSE
AccessioniPrimary (citable) accession number: Q925D8
Secondary accession number(s): A2ADA0
, Q91VA4, Q923K0, Q925A4, Q925D9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: January 4, 2005
Last modified: September 7, 2016
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.