Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Taste receptor type 1 member 3

Gene

Tas1r3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Putative taste receptor. TAS1R1/TAS1R3 responds to the umami taste stimulus (the taste of monosodium glutamate) and also to most of the 20 standard L-amino acids, but not to their D-enantiomers or other compounds. TAS1R2/TAS1R3 recognizes diverse natural and synthetic sweeteners. TAS1R3 is essential for the recognition and response to the disaccharide trehalose. Sequence differences within and between species can significantly influence the selectivity and specificity of taste responses.2 Publications

GO - Molecular functioni

  • G-protein coupled receptor activity Source: MGI
  • sweet taste receptor activity Source: Ensembl
  • taste receptor activity Source: MGI

GO - Biological processi

  • detection of chemical stimulus involved in sensory perception of sweet taste Source: MGI
  • G-protein coupled receptor signaling pathway Source: MGI
  • sensory perception of sweet taste Source: MGI
  • sensory perception of umami taste Source: MGI

Keywordsi

Molecular functionG-protein coupled receptor, Receptor, Transducer
Biological processSensory transduction, Taste

Enzyme and pathway databases

ReactomeiR-MMU-418594 G alpha (i) signalling events
R-MMU-420499 Class C/3 (Metabotropic glutamate/pheromone receptors)

Names & Taxonomyi

Protein namesi
Recommended name:
Taste receptor type 1 member 3
Alternative name(s):
Saccharin preference protein
Sweet taste receptor T1R3
Gene namesi
Name:Tas1r3
Synonyms:Sac, T1r3, Tr3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1933547 Tas1r3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini21 – 572ExtracellularSequence analysisAdd BLAST552
Transmembranei573 – 593Helical; Name=1Sequence analysisAdd BLAST21
Topological domaini594 – 610CytoplasmicSequence analysisAdd BLAST17
Transmembranei611 – 631Helical; Name=2Sequence analysisAdd BLAST21
Topological domaini632 – 644ExtracellularSequence analysisAdd BLAST13
Transmembranei645 – 665Helical; Name=3Sequence analysisAdd BLAST21
Topological domaini666 – 687CytoplasmicSequence analysisAdd BLAST22
Transmembranei688 – 708Helical; Name=4Sequence analysisAdd BLAST21
Topological domaini709 – 735ExtracellularSequence analysisAdd BLAST27
Transmembranei736 – 756Helical; Name=5Sequence analysisAdd BLAST21
Topological domaini757 – 767CytoplasmicSequence analysisAdd BLAST11
Transmembranei768 – 788Helical; Name=6Sequence analysisAdd BLAST21
Topological domaini789 – 796ExtracellularSequence analysis8
Transmembranei797 – 817Helical; Name=7Sequence analysisAdd BLAST21
Topological domaini818 – 858CytoplasmicSequence analysisAdd BLAST41

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
ChainiPRO_000001296221 – 858Taste receptor type 1 member 3Add BLAST838

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi58N-linked (GlcNAc...) asparagine; when associated with variant T-60Sequence analysis1
Glycosylationi85N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi130N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi203N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi264N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi379N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi387N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi418N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi439N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi482N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

The Thr-60 variant is predicted to introduce a novel N-linked glycosylation site at Asn-58. The addition of even a short carbohydrate group at Asn-58 is predicted to disrupt one of the contact surfaces required for stability of a dimer. Therefore a Thr-60 variant N-glycosylated at Asn-58 is predicted to be precluded from forming homodimers or heterodimers.

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ925D8
PRIDEiQ925D8

Expressioni

Tissue specificityi

Expressed in circumvallate, foliate and fungiform taste papillae as well as in taste buds on the palate. Also expressed in testis. Not expressed in brain, heart, kidney, liver or spleen. The topographic distribution in various taste papillae is different from those of other T1R members.4 Publications

Gene expression databases

BgeeiENSMUSG00000029072
CleanExiMM_TAS1R3
GenevisibleiQ925D8 MM

Interactioni

Subunit structurei

Forms homodimers or heterodimers with TAS1R1 and TAS1R2.

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000030949

Structurei

3D structure databases

ProteinModelPortaliQ925D8
SMRiQ925D8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1056 Eukaryota
ENOG410XR6W LUCA
GeneTreeiENSGT00760000118884
HOGENOMiHOG000231293
HOVERGENiHBG098572
InParanoidiQ925D8
KOiK04626
OMAiCFGLACL
OrthoDBiEOG091G01UW
PhylomeDBiQ925D8
TreeFamiTF331269

Family and domain databases

Gene3Di2.10.50.30, 1 hit
InterProiView protein in InterPro
IPR001828 ANF_lig-bd_rcpt
IPR000337 GPCR_3
IPR011500 GPCR_3_9-Cys_dom
IPR038550 GPCR_3_9-Cys_sf
IPR017978 GPCR_3_C
IPR017979 GPCR_3_CS
IPR028082 Peripla_BP_I
PfamiView protein in Pfam
PF00003 7tm_3, 1 hit
PF01094 ANF_receptor, 1 hit
PF07562 NCD3G, 1 hit
PRINTSiPR00248 GPCRMGR
SUPFAMiSSF53822 SSF53822, 1 hit
PROSITEiView protein in PROSITE
PS00980 G_PROTEIN_RECEP_F3_2, 1 hit
PS50259 G_PROTEIN_RECEP_F3_4, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q925D8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPALAIMGLS LAAFLELGMG ASLCLSQQFK AQGDYILGGL FPLGSTEEAT
60 70 80 90 100
LNQRTQPNSI PCNRFSPLGL FLAMAMKMAV EEINNGSALL PGLRLGYDLF
110 120 130 140 150
DTCSEPVVTM KSSLMFLAKV GSQSIAAYCN YTQYQPRVLA VIGPHSSELA
160 170 180 190 200
LITGKFFSFF LMPQVSYSAS MDRLSDRETF PSFFRTVPSD RVQLQAVVTL
210 220 230 240 250
LQNFSWNWVA ALGSDDDYGR EGLSIFSSLA NARGICIAHE GLVPQHDTSG
260 270 280 290 300
QQLGKVLDVL RQVNQSKVQV VVLFASARAV YSLFSYSIHH GLSPKVWVAS
310 320 330 340 350
ESWLTSDLVM TLPNIARVGT VLGFLQRGAL LPEFSHYVET HLALAADPAF
360 370 380 390 400
CASLNAELDL EEHVMGQRCP RCDDIMLQNL SSGLLQNLSA GQLHHQIFAT
410 420 430 440 450
YAAVYSVAQA LHNTLQCNVS HCHVSEHVLP WQLLENMYNM SFHARDLTLQ
460 470 480 490 500
FDAEGNVDME YDLKMWVWQS PTPVLHTVGT FNGTLQLQQS KMYWPGNQVP
510 520 530 540 550
VSQCSRQCKD GQVRRVKGFH SCCYDCVDCK AGSYRKHPDD FTCTPCNQDQ
560 570 580 590 600
WSPEKSTACL PRRPKFLAWG EPVVLSLLLL LCLVLGLALA ALGLSVHHWD
610 620 630 640 650
SPLVQASGGS QFCFGLICLG LFCLSVLLFP GRPSSASCLA QQPMAHLPLT
660 670 680 690 700
GCLSTLFLQA AETFVESELP LSWANWLCSY LRGLWAWLVV LLATFVEAAL
710 720 730 740 750
CAWYLIAFPP EVVTDWSVLP TEVLEHCHVR SWVSLGLVHI TNAMLAFLCF
760 770 780 790 800
LGTFLVQSQP GRYNRARGLT FAMLAYFITW VSFVPLLANV QVAYQPAVQM
810 820 830 840 850
GAILVCALGI LVTFHLPKCY VLLWLPKLNT QEFFLGRNAK KAADENSGGG

EAAQGHNE
Length:858
Mass (Da):94,561
Last modified:January 4, 2005 - v2
Checksum:i4E4168279DB478F8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti184F → L in AAK55537 (PubMed:11326277).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti55T → A in strain: 129/J, 129/SvEv, AKR/J, BALB/c, C3H/HeJ, DBA/2J and DBA/2J. 8 Publications1
Natural varianti60I → T in strain: 129/J, 129/SvEv, AKR/J, BALB/c, C3H/HeJ, DBA/2J and DBA/2J; may influence the ability to form dimers or bind sweeteners. 8 Publications1
Natural varianti61P → L in strain: 129/J, 129/SvEv, AKR/J, BALB/c, C3H/HeJ, CAST/Ei, DBA/2J, FVB/N, ST/bJ and SWR/J. 7 Publications1
Natural varianti261R → C in strain: FVB/N, ST/bJ, SWR/J. 4 Publications1
Natural varianti371R → Q in strain: 129/J, 129/SvEv, AKR/J, BALB/c, CAST/Ei, C3H/HeJ, DBA/2J, FVB/N, ST/bJ and SWR/J. 6 Publications1
Natural varianti692L → S in strain: FVB/N, ST/bJ and SWR/J. 4 Publications1
Natural varianti706I → N in strain: SWR/J. 2 Publications1
Natural varianti706I → T in strain: 129/J, 129/SvEv, AKR/J, BALB/c, C3H/HeJ, DBA/2J, DBA/2J, FVB/N, ST/bJ and SWR/J. 6 Publications1
Natural varianti855G → E in strain: 129/J, AKR/J, CAST/Ei, DBA/2J and SWR/J. 4 Publications1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB049994 mRNA Translation: BAB47181.1
AB055708 Genomic DNA Translation: BAB62852.1
AF337039 mRNA Translation: AAK39436.1
AF311386 mRNA Translation: AAL08425.1
AY032621 mRNA Translation: AAK51602.1
AY026318 Genomic DNA Translation: AAK01937.1
AF368024 Genomic DNA Translation: AAK55536.1
AF368025 Genomic DNA Translation: AAK55537.1
AL670236 Genomic DNA Translation: CAM18393.1
CCDSiCCDS19046.1
PIRiJC7683
RefSeqiNP_114078.1, NM_031872.2
UniGeneiMm.358792

Genome annotation databases

EnsembliENSMUST00000030949; ENSMUSP00000030949; ENSMUSG00000029072
GeneIDi83771
KEGGimmu:83771
UCSCiuc008wff.1 mouse

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiTS1R3_MOUSE
AccessioniPrimary (citable) accession number: Q925D8
Secondary accession number(s): A2ADA0
, Q91VA4, Q923K0, Q925A4, Q925D9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: January 4, 2005
Last modified: March 28, 2018
This is version 119 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health