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Q925D8 (TS1R3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Taste receptor type 1 member 3
Alternative name(s):
Saccharin preference protein
Sweet taste receptor T1R3
Gene names
Name:Tas1r3
Synonyms:Sac, T1r3, Tr3
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length858 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Putative taste receptor. TAS1R1/TAS1R3 responds to the umami taste stimulus (the taste of monosodium glutamate) and also to most of the 20 standard L-amino acids, but not to their D-enantiomers or other compounds. TAS1R2/TAS1R3 recognizes diverse natural and synthetic sweeteners. TAS1R3 is essential for the recognition and response to the disaccharide trehalose. Sequence differences within and between species can significantly influence the selectivity and specificity of taste responses. Ref.7 Ref.10

Subunit structure

Forms homodimers or heterodimers with TAS1R1 and TAS1R2.

Subcellular location

Cell membrane; Multi-pass membrane protein.

Tissue specificity

Expressed in circumvallate, foliate and fungiform taste papillae as well as in taste buds on the palate. Also expressed in testis. Not expressed in brain, heart, kidney, liver or spleen. The topographic distribution in various taste papillae is different from those of other T1R members. Ref.1 Ref.2 Ref.5 Ref.8

Post-translational modification

The Thr-60 variant is predicted to introduce a novel N-linked glycosylation site at Asn-58. The addition of even a short carbohydrate group at Asn-58 is predicted to disrupt one of the contact surfaces required for stability of a dimer. Therefore a Thr-60 variant N-glycosylated at Asn-58 is predicted to be precluded from forming homodimers or heterodimers.

Sequence similarities

Belongs to the G-protein coupled receptor 3 family. TAS1R subfamily.

Ontologies

Keywords
   Biological processSensory transduction
Taste
   Cellular componentCell membrane
Membrane
   Coding sequence diversityPolymorphism
   DomainSignal
Transmembrane
Transmembrane helix
   Molecular functionG-protein coupled receptor
Receptor
Transducer
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular componentintegral to plasma membrane

Traceable author statement Ref.3. Source: MGI

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2020 Potential
Chain21 – 858838Taste receptor type 1 member 3
PRO_0000012962

Regions

Topological domain21 – 572552Extracellular Potential
Transmembrane573 – 59321Helical; Name=1; Potential
Topological domain594 – 61017Cytoplasmic Potential
Transmembrane611 – 63121Helical; Name=2; Potential
Topological domain632 – 64413Extracellular Potential
Transmembrane645 – 66521Helical; Name=3; Potential
Topological domain666 – 68722Cytoplasmic Potential
Transmembrane688 – 70821Helical; Name=4; Potential
Topological domain709 – 73527Extracellular Potential
Transmembrane736 – 75621Helical; Name=5; Potential
Topological domain757 – 76711Cytoplasmic Potential
Transmembrane768 – 78821Helical; Name=6; Potential
Topological domain789 – 7968Extracellular Potential
Transmembrane797 – 81721Helical; Name=7; Potential
Topological domain818 – 85841Cytoplasmic Potential

Amino acid modifications

Glycosylation581N-linked (GlcNAc...); when associated with variant T-60 Potential
Glycosylation851N-linked (GlcNAc...) Potential
Glycosylation1301N-linked (GlcNAc...) Potential
Glycosylation2031N-linked (GlcNAc...) Potential
Glycosylation2641N-linked (GlcNAc...) Potential
Glycosylation3791N-linked (GlcNAc...) Potential
Glycosylation3871N-linked (GlcNAc...) Potential
Glycosylation4181N-linked (GlcNAc...) Potential
Glycosylation4391N-linked (GlcNAc...) Potential
Glycosylation4821N-linked (GlcNAc...) Potential

Natural variations

Natural variant551T → A in strain: 129/J, 129/SvEv, AKR/J, BALB/c, C3H/HeJ, DBA/2J and DBA/2J. Ref.1 Ref.2 Ref.3 Ref.4 Ref.5 Ref.6 Ref.8 Ref.13
Natural variant601I → T in strain: 129/J, 129/SvEv, AKR/J, BALB/c, C3H/HeJ, DBA/2J and DBA/2J; may influence the ability to form dimers or bind sweeteners. Ref.1 Ref.2 Ref.3 Ref.4 Ref.5 Ref.6 Ref.8 Ref.13
Natural variant611P → L in strain: 129/J, 129/SvEv, AKR/J, BALB/c, C3H/HeJ, CAST/Ei, DBA/2J, FVB/N, ST/bJ and SWR/J. Ref.1 Ref.2 Ref.4 Ref.5 Ref.6 Ref.8 Ref.13
Natural variant2611R → C in strain: FVB/N, ST/bJ, SWR/J. Ref.2 Ref.4 Ref.8 Ref.13
Natural variant3711R → Q in strain: 129/J, 129/SvEv, AKR/J, BALB/c, CAST/Ei, C3H/HeJ, DBA/2J, FVB/N, ST/bJ and SWR/J. Ref.1 Ref.2 Ref.4 Ref.5 Ref.8 Ref.13
Natural variant6921L → S in strain: FVB/N, ST/bJ and SWR/J. Ref.2 Ref.4 Ref.8 Ref.13
Natural variant7061I → N in strain: SWR/J. Ref.7 Ref.13
Natural variant7061I → T in strain: 129/J, 129/SvEv, AKR/J, BALB/c, C3H/HeJ, DBA/2J, DBA/2J, FVB/N, ST/bJ and SWR/J. Ref.1 Ref.2 Ref.4 Ref.5 Ref.8 Ref.13
Natural variant8551G → E in strain: 129/J, AKR/J, CAST/Ei, DBA/2J and SWR/J. Ref.2 Ref.4 Ref.5 Ref.13

Experimental info

Sequence conflict1841F → L in AAK55537. Ref.8

Sequences

Sequence LengthMass (Da)Tools
Q925D8 [UniParc].

Last modified January 4, 2005. Version 2.
Checksum: 4E4168279DB478F8

FASTA85894,561
        10         20         30         40         50         60 
MPALAIMGLS LAAFLELGMG ASLCLSQQFK AQGDYILGGL FPLGSTEEAT LNQRTQPNSI 

        70         80         90        100        110        120 
PCNRFSPLGL FLAMAMKMAV EEINNGSALL PGLRLGYDLF DTCSEPVVTM KSSLMFLAKV 

       130        140        150        160        170        180 
GSQSIAAYCN YTQYQPRVLA VIGPHSSELA LITGKFFSFF LMPQVSYSAS MDRLSDRETF 

       190        200        210        220        230        240 
PSFFRTVPSD RVQLQAVVTL LQNFSWNWVA ALGSDDDYGR EGLSIFSSLA NARGICIAHE 

       250        260        270        280        290        300 
GLVPQHDTSG QQLGKVLDVL RQVNQSKVQV VVLFASARAV YSLFSYSIHH GLSPKVWVAS 

       310        320        330        340        350        360 
ESWLTSDLVM TLPNIARVGT VLGFLQRGAL LPEFSHYVET HLALAADPAF CASLNAELDL 

       370        380        390        400        410        420 
EEHVMGQRCP RCDDIMLQNL SSGLLQNLSA GQLHHQIFAT YAAVYSVAQA LHNTLQCNVS 

       430        440        450        460        470        480 
HCHVSEHVLP WQLLENMYNM SFHARDLTLQ FDAEGNVDME YDLKMWVWQS PTPVLHTVGT 

       490        500        510        520        530        540 
FNGTLQLQQS KMYWPGNQVP VSQCSRQCKD GQVRRVKGFH SCCYDCVDCK AGSYRKHPDD 

       550        560        570        580        590        600 
FTCTPCNQDQ WSPEKSTACL PRRPKFLAWG EPVVLSLLLL LCLVLGLALA ALGLSVHHWD 

       610        620        630        640        650        660 
SPLVQASGGS QFCFGLICLG LFCLSVLLFP GRPSSASCLA QQPMAHLPLT GCLSTLFLQA 

       670        680        690        700        710        720 
AETFVESELP LSWANWLCSY LRGLWAWLVV LLATFVEAAL CAWYLIAFPP EVVTDWSVLP 

       730        740        750        760        770        780 
TEVLEHCHVR SWVSLGLVHI TNAMLAFLCF LGTFLVQSQP GRYNRARGLT FAMLAYFITW 

       790        800        810        820        830        840 
VSFVPLLANV QVAYQPAVQM GAILVCALGI LVTFHLPKCY VLLWLPKLNT QEFFLGRNAK 

       850 
KAADENSGGG EAAQGHNE

« Hide

References

« Hide 'large scale' references
[1]"Molecular genetic identification of a candidate receptor gene for sweet taste."
Kitagawa M., Kusakabe Y., Miura H., Ninomiya Y., Hino A.
Biochem. Biophys. Res. Commun. 283:236-242(2001) [PubMed: 11322794] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, VARIANTS ALA-55; THR-60; LEU-61; GLN-371 AND THR-706.
Strain: C57BL/6N and C57BL/6NCr.
Tissue: Brain and Circumvallate papilla.
[2]"A candidate taste receptor gene near a sweet taste locus."
Montmayeur J.-P., Liberles S.D., Matsunami H., Buck L.B.
Nat. Neurosci. 4:492-498(2001) [PubMed: 11319557] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, VARIANTS ALA-55; THR-60; LEU-61; CYS-261; GLN-371; SER-692; THR-706 AND GLU-855.
Strain: C57BL/6J, DBA/2J and SWR/J.
Tissue: Circumvallate papilla and Foliate papilla.
[3]"Positional cloning of the mouse saccharin preference (Sac) locus."
Bachmanov A.A., Li X., Reed D.R., Ohmen J.D., Li S., Chen Z., Tordoff M.G., de Jong P.J., Wu C., West D.B., Chatterjee A., Ross D.A., Beauchamp G.K.
Chem. Senses 26:925-933(2001) [PubMed: 11555487] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS ALA-55 AND THR-60.
Strain: 129/J, AKR/J, BALB/cByJ, C3H/HeJ, C57BL/6ByJ, C57L/J, CAST/Ei, CBA/J, DBA/2J, IS/CamEi, SEA/GnJ, ST/bJ and SWR/J.
[4]"Mammalian sweet taste receptors."
Nelson G., Hoon M.A., Chandrashekar J., Zhang Y., Ryba N.J.P., Zuker C.S.
Cell 106:381-390(2001) [PubMed: 11509186] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS ALA-55; THR-60; LEU-61; CYS-261; GLN-371; SER-692; THR-706 AND GLU-855.
Strain: 129/Sv, AKR/J, BALB/c, C3H/HeJ, C57BL/6, C57L/J, DBA/1LacJ, DBA/2J, FVB/N, ST/bJ and SWR/J.
[5]"Identification of a novel member of the T1R family of putative taste receptors."
Sainz E., Korley J.N., Battey J.F., Sullivan S.L.
J. Neurochem. 77:896-903(2001) [PubMed: 11331418] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE, TISSUE SPECIFICITY, VARIANTS ALA-55; THR-60; LEU-61; GLN-371; THR-706 AND GLU-855.
Strain: 129/Sv and C57BL/6.
[6]"Genetic, physical, and comparative map of the subtelomeric region of mouse chromosome 4."
Li X., Bachmanov A.A., Li S., Chen Z., Tordoff M.G., Beauchamp G.K., de Jong P.J., Wu C., Chen L., West D.B., Ross D.A., Ohmen J.D., Reed D.R.
Mamm. Genome 13:5-19(2002) [PubMed: 11773963] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS ALA-55; THR-60 AND LEU-61.
Strain: 129/SvEv and C57BL/6J.
[7]"Taste receptor T1R3 is an essential molecule for the cellular recognition of the disaccharide trehalose."
Ariyasu T., Matsumoto S., Kyono F., Hanaya T., Arai S., Ikeda M., Kurimoto M.
In Vitro Cell. Dev. Biol. Anim. 39:80-88(2003) [PubMed: 12892531] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE, FUNCTION, VARIANT ASN-706.
Strain: C57BL/6.
[8]"Tas1r3, encoding a new candidate taste receptor, is allelic to the sweet responsiveness locus Sac."
Max M., Shanker Y.G., Huang L., Rong M., Liu Z., Campagne F., Weinstein H., Damak S., Margolskee R.F.
Nat. Genet. 28:58-63(2001) [PubMed: 11326277] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS ALA-55; THR-60; LEU-61; CYS-261; GLN-371; SER-692 AND THR-706, TISSUE SPECIFICITY.
Strain: 129/SvEv, BALB/c, C3H/HeJ, C57BL/6, DBA/2J, FVB/N, ST/bJ and SWR/J.
[9]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed: 19468303] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6.
[10]"An amino-acid taste receptor."
Nelson G., Chandrashekar J., Hoon M.A., Feng L., Zhao G., Ryba N.J.P., Zuker C.S.
Nature 416:199-202(2002) [PubMed: 11894099] [Abstract]
Cited for: FUNCTION.
[11]"The receptors for mammalian sweet and umami taste."
Zhao G.Q., Zhang Y., Hoon M.A., Chandrashekar J., Erlenbach I., Ryba N.J.P., Zuker C.S.
Cell 115:255-266(2003) [PubMed: 14636554] [Abstract]
Cited for: SWEET AND UMAMI TASTES EXCLUSIVELY MEDIATED BY TAS1R RECEPTORS.
[12]"Detection of sweet and umami taste in the absence of taste receptor T1r3."
Damak S., Rong M., Yasumatsu K., Kokrashvili Z., Varadarajan V., Zou S., Jiang P., Ninomiya Y., Margolskee R.F.
Science 301:850-853(2003) [PubMed: 12869700] [Abstract]
Cited for: TAS1R3 INDEPENDENT SWEET- AND UMAMI-RESPONSIVE RECEPTORS.
[13]"Polymorphisms in the taste receptor gene (Tas1r3) region are associated with saccharin preference in 30 mouse strains."
Reed D.R., Li S., Li X., Huang L., Tordoff M.G., Starling-Roney R., Taniguchi K., West D.B., Ohmen J.D., Beauchamp G.K., Bachmanov A.A.
J. Neurosci. 24:938-946(2004) [PubMed: 14749438] [Abstract]
Cited for: VARIANTS ALA-55; THR-60; LEU-61; CYS-261; GLN-371; SER-692; ASN-706; THR-706 AND GLU-855.
Strain: 129/J, AKR/J, C57BL/6, CAST/Ei, DBA/2J and SWR/J.
[14]"Allelic variation of the Tas1r3 taste receptor gene selectively affects behavioral and neural taste responses to sweeteners in the F2 hybrids between C57BL/6ByJ and 129P3/J mice."
Inoue M., Reed D.R., Li X., Tordoff M.G., Beauchamp G.K., Bachmanov A.A.
J. Neurosci. 24:2296-2303(2004) [PubMed: 14999080] [Abstract]
Cited for: ALLELIC VARIATION RESPONSE TO SWEETENERS.
Strain: 129/J and C57BL/ByJ.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AB049994 mRNA. Translation: BAB47181.1.
AB055708 Genomic DNA. Translation: BAB62852.1.
AF337039 mRNA. Translation: AAK39436.1.
AF311386 mRNA. Translation: AAL08425.1.
AY032621 mRNA. Translation: AAK51602.1.
AY026318 Genomic DNA. Translation: AAK01937.1.
AF368024 Genomic DNA. Translation: AAK55536.1.
AF368025 Genomic DNA. Translation: AAK55537.1.
AL670236 Genomic DNA. Translation: CAM18393.1.
IPIIPI00387331.
PIRJC7683.
RefSeqNP_114078.1. NM_031872.2.
UniGeneMm.358792.

3D structure databases

ProteinModelPortalQ925D8.
SMRQ925D8. Positions 26-564.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ925D8.

Protein family/group databases

GPCRDBSearch...

Proteomic databases

PRIDEQ925D8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000030949; ENSMUSP00000030949; ENSMUSG00000029072.
GeneID83771.
KEGGmmu:83771.
UCSCuc008wff.1. mouse.

Organism-specific databases

CTD83756.
MGIMGI:1933547. Tas1r3.

Phylogenomic databases

GeneTreeENSGT00580000081472.
HOGENOMHBG447320.
HOVERGENHBG098572.
InParanoidQ925D8.
OMAHLPLTGC.
OrthoDBEOG44XJG8.
PhylomeDBQ925D8.

Gene expression databases

ArrayExpressQ925D8.
BgeeQ925D8.
CleanExMM_TAS1R3.
GenevestigatorQ925D8.
GermOnlineENSMUSG00000029072. Mus musculus.

Family and domain databases

InterProIPR001828. ANF_lig-bd_rcpt.
IPR000337. GPCR_3.
IPR011500. GPCR_3_9-Cys_dom.
IPR017978. GPCR_3_C.
IPR017979. GPCR_3_CS.
[Graphical view]
KOK04626.
PfamPF00003. 7tm_3. 1 hit.
PF01094. ANF_receptor. 1 hit.
PF07562. NCD3G. 1 hit.
[Graphical view]
PRINTSPR00248. GPCRMGR.
PROSITEPS00979. G_PROTEIN_RECEP_F3_1. False negative.
PS00980. G_PROTEIN_RECEP_F3_2. 1 hit.
PS00981. G_PROTEIN_RECEP_F3_3. False negative.
PS50259. G_PROTEIN_RECEP_F3_4. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio350772.
SOURCESearch...

Entry information

Entry nameTS1R3_MOUSE
AccessionPrimary (citable) accession number: Q925D8
Secondary accession number(s): A2ADA0 expand/collapse secondary AC list , Q91VA4, Q923K0, Q925A4, Q925D9
Entry history
Integrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: January 4, 2005
Last modified: November 16, 2011
This is version 79 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents