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Protein

Transient receptor potential cation channel subfamily M member 7

Gene

Trpm7

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Essential ion channel and serine/threonine-protein kinase. Divalent cation channel permeable to calcium and magnesium. Has a central role in magnesium ion homeostasis and in the regulation of anoxic neuronal cell death. Involved in TNF-induced necroptosis downstream of MLKL by mediating calcium influx. The kinase activity is essential for the channel function. May be involved in a fundamental process that adjusts plasma membrane divalent cation fluxes according to the metabolic state of the cell. Phosphorylates annexin A1 (ANXA1) (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.By similarity

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei1621ATPBy similarity1
Binding sitei1645ATPBy similarity1
Binding sitei1717ATPBy similarity1
Metal bindingi1750ZincBy similarity1
Active sitei1764Proton acceptorBy similarity1
Binding sitei1766ATPBy similarity1
Binding sitei1774ATPBy similarity1
Metal bindingi1807ZincBy similarity1
Metal bindingi1809ZincBy similarity1
Metal bindingi1813ZincBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi1791 – 1797ATPBy similarity7

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • calcium channel activity Source: RGD
  • cation channel activity Source: RGD
  • metal ion binding Source: UniProtKB-KW
  • protein kinase activity Source: RGD
  • protein serine/threonine kinase activity Source: UniProtKB-KW

GO - Biological processi

  • calcium ion transport Source: RGD
  • cellular magnesium ion homeostasis Source: RGD
  • memory Source: RGD
  • necroptotic process Source: UniProtKB
  • positive regulation of apoptotic process Source: RGD
  • protein tetramerization Source: InterPro
  • response to radiation Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Calcium channel, Ion channel, Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Calcium transport, Ion transport, Necrosis, Transport

Keywords - Ligandi

ATP-binding, Calcium, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Transient receptor potential cation channel subfamily M member 7Imported (EC:2.7.11.1)
Alternative name(s):
Long transient receptor potential channel 7By similarity
Short name:
LTrpC-7
Short name:
LTrpC7By similarity
Transient receptor potential-phospholipase C-interacting kinase1 Publication
Short name:
TRP-PLIK1 Publication
Gene namesi
Name:Trpm7Imported
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620053. Trpm7.

Subcellular locationi

  • Membrane By similarity; Multi-pass membrane protein By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 755CytoplasmicSequence analysisAdd BLAST755
Transmembranei756 – 776HelicalSequence analysisAdd BLAST21
Topological domaini777 – 855ExtracellularSequence analysisAdd BLAST79
Transmembranei856 – 876HelicalSequence analysisAdd BLAST21
Topological domaini877 – 918CytoplasmicSequence analysisAdd BLAST42
Transmembranei919 – 939HelicalSequence analysisAdd BLAST21
Topological domaini940 – 962ExtracellularSequence analysisAdd BLAST23
Transmembranei963 – 983HelicalSequence analysisAdd BLAST21
Topological domaini984 – 995CytoplasmicSequence analysisAdd BLAST12
Transmembranei996 – 1016HelicalSequence analysisAdd BLAST21
Topological domaini1017 – 1035ExtracellularSequence analysisAdd BLAST19
Intramembranei1036 – 1056Pore-formingSequence analysisAdd BLAST21
Topological domaini1057 – 1074ExtracellularSequence analysisAdd BLAST18
Transmembranei1075 – 1095HelicalSequence analysisAdd BLAST21
Topological domaini1096 – 1862CytoplasmicSequence analysisAdd BLAST767

GO - Cellular componenti

  • cytoplasm Source: RGD
  • integral component of membrane Source: RGD
  • neuronal cell body Source: RGD
  • neuron projection Source: RGD
  • plasma membrane Source: RGD
  • synaptic vesicle membrane Source: RGD
  • varicosity Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Chemistry databases

GuidetoPHARMACOLOGYi499.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003546641 – 1862Transient receptor potential cation channel subfamily M member 7Add BLAST1862

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei101PhosphoserineBy similarity1
Modified residuei1224PhosphoserineBy similarity1
Modified residuei1255PhosphoserineBy similarity1
Modified residuei1300PhosphoserineBy similarity1
Modified residuei1385PhosphoserineBy similarity1
Modified residuei1386PhosphoserineBy similarity1
Modified residuei1389PhosphoserineBy similarity1
Modified residuei1394PhosphoserineBy similarity1
Modified residuei1403PhosphoserineBy similarity1
Modified residuei1404PhosphothreonineBy similarity1
Modified residuei1445PhosphoserineBy similarity1
Modified residuei1466PhosphothreonineBy similarity1
Modified residuei1497PhosphoserineBy similarity1
Modified residuei1501PhosphoserineBy similarity1
Modified residuei1566PhosphoserineBy similarity1
Modified residuei1848PhosphoserineBy similarity1

Post-translational modificationi

Autophosphorylated.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ925B3.
PRIDEiQ925B3.

PTM databases

iPTMnetiQ925B3.
PhosphoSitePlusiQ925B3.
SwissPalmiQ925B3.

Interactioni

Subunit structurei

Homodimer (By similarity). Forms heterodimers with TRPM6 (By similarity). Interacts with PLCB1.By similarity1 Publication

Protein-protein interaction databases

IntActiQ925B3. 1 interactor.
MINTiMINT-2778884.
STRINGi10116.ENSRNOP00000034169.

Structurei

Secondary structure

11862
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi1198 – 1247Combined sources50

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3E7KX-ray2.01A/B/C/D/E/F/G/H1198-1249[»]
SMRiQ925B3.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ925B3.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1591 – 1821Alpha-type protein kinasePROSITE-ProRule annotationAdd BLAST231

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili1198 – 12501 PublicationAdd BLAST53

Sequence similaritiesi

In the C-terminal section; belongs to the protein kinase superfamily. Alpha-type protein kinase family. ALPK subfamily.Sequence analysis
Contains 1 alpha-type protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3614. Eukaryota.
ENOG410XR5B. LUCA.
HOGENOMiHOG000230920.
HOVERGENiHBG055663.
InParanoidiQ925B3.
PhylomeDBiQ925B3.

Family and domain databases

InterProiIPR005821. Ion_trans_dom.
IPR011009. Kinase-like_dom.
IPR004166. MHCK_EF2_kinase.
IPR029601. TRPM7.
IPR032415. TRPM_tetra.
[Graphical view]
PANTHERiPTHR13800:SF8. PTHR13800:SF8. 3 hits.
PfamiPF02816. Alpha_kinase. 1 hit.
PF00520. Ion_trans. 1 hit.
PF16519. TRPM_tetra. 1 hit.
[Graphical view]
SMARTiSM00811. Alpha_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS51158. ALPHA_KINASE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1Curated (identifier: Q925B3-1) [UniParc]FASTAAdd to basket
Also known as: TRP-PLIK1 Publication

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSQKSWIEST LTKRECVYII PSSKDPHRCL PGCQICQQLV RCFCGRLVKQ
60 70 80 90 100
HACFTASLAT KYSDVKLGEH FNQAIEEWSV EKHTEQSPTD AYGVINFQGG
110 120 130 140 150
SHSYRAKYVR LSYDTKPEII LQLLLKEWQM ELPKLVISVH GGMQKFELHP
160 170 180 190 200
RIKQLLGKGL IKAAVTTGAW ILTGGVNTGV AKHVGDALKE HASRSSRKIC
210 220 230 240 250
TIGIAPWGVI ENRNDLVGRD VVAPYQTLLN PLSKLNVLNN LHSHFILVDD
260 270 280 290 300
GTVGKYGAEV RLRRELEKTI NQQRIHARIG QGVPVVALIF EGGPNVILTV
310 320 330 340 350
LEYLQESPPV PVVVCEGTGR AADLLAYIHK QTEEGGNLPD AAEPDIISTI
360 370 380 390 400
KKTFNFGQSE AVHLFQTMME CMKKKELITV FHIGSEDHQD IDVAILTALX
410 420 430 440 450
XGTNASAFDQ LILTLAWDRV DIAKNHVFVY GQQWLVGSLE QAMLDALVMD
460 470 480 490 500
RVSFVKLLIE NGVSMHKFLT IPRLEELYNT KQGPTNPMLF HLIRDVKQGN
510 520 530 540 550
LPPGYKITLI DIGLVIEYLM GGTYRCTYTR KRFRLIYNSL GGNNRRSGRN
560 570 580 590 600
ASSSTPQLRK SHETFGNRAD KKEKMRHNHF IKTAQPYRPK MDASMEEGKK
610 620 630 640 650
KRTKDEIVDI DDPETKRFPY PLNELLIWAC LMKRQVMARF LWQHGEESMA
660 670 680 690 700
KALVACKIYR SMAYEAKQSD LVDDTSEELK QYSNDFGQLA VELLEQSFRQ
710 720 730 740 750
DETMAMKLLT YELKNWSNST CLKLAVSSRL RPFVAHTCTQ MLLSDMWMGR
760 770 780 790 800
LNMRKNSWYK VILSILVPPA ILMLEYKTKA EMSHIPQSQD AHQMTMEDSE
810 820 830 840 850
NNFHNITEEI PMEVFKEVKI LDSSEGKNEM EIHIKSKKLP ITRKFYAFYH
860 870 880 890 900
APIVKFWFNT LAYLGFLMLY TFVVLVQMEQ LPSVQEWIVI AYIFTYAIEK
910 920 930 940 950
IREVFMSEAG KISQKIKVWF SDYFNVSDTI AIISFFVGFG LRFGAKWNYI
960 970 980 990 1000
NAYDNHVFVA GRLIYCLNII FWYVRLLDFL AVNQQAGPYV MMIGKMVANM
1010 1020 1030 1040 1050
FYIVVIMALV LLSFGVPRKA ILYPHEEPSW SLAKDIVFHP YWMIFGEVYA
1060 1070 1080 1090 1100
YEIDVCANDS ALPTICGPGT WLTPFLQAVY LFVQYIIMVN LLIAFFNNVY
1110 1120 1130 1140 1150
LQVKAISNIV WKYQRYHFIM AYHEKPVLPP PLIILSHIVS LFCCICKRRK
1160 1170 1180 1190 1200
KDKTSDGPKL FLTEEDQKKL HDFEEQCVEM YFDEKDDKFN SGSEERIRVT
1210 1220 1230 1240 1250
FERVEQMSIQ IKEVGDRVNY IKRSLQSLDS QIGHLQDLSA LTVDTLKTLT
1260 1270 1280 1290 1300
AQKASEASKV HNEITRELSI SKHLAQNLID DVPVRPMWKK PSVVNTLSSS
1310 1320 1330 1340 1350
LPQGDRESNN PFLCNIFMKD EKDPQYNLFG QDLPVIPQRK EFNIPEAGSS
1360 1370 1380 1390 1400
CGALFPSAVS PPELRQRRHG VEMLKIFNKN QKLGSSPNSS PHMSSPPTKF
1410 1420 1430 1440 1450
SVSTPSQPSC KSHLESTTKD PEPIFYKAAE GDNIEFGAFV GHRDSMDLQR
1460 1470 1480 1490 1500
FKETSNKIRE LLSNDTPENT LKHVGAAGYN ECHKTPTSLH SETESCSRRA
1510 1520 1530 1540 1550
STEDSPDVDS RAALLPDWLR DRPTNREMPS EGGTLNGLAS PFKPVLDTNY
1560 1570 1580 1590 1600
YYSAVERNNL MRLSQSIPFV PVPPRGEPVT VYRLEESSPS ILNNSMSSWS
1610 1620 1630 1640 1650
QLGLCAKIEF LSKEEMGGGL RRAVKVLCTW SEHDVLRSGH LYIIKSFLPE
1660 1670 1680 1690 1700
VINTWSSIYK EDTVLHLCLR EIQQQRAAQK LTFAFNQMKP KSIPYSPRFL
1710 1720 1730 1740 1750
EVFLLYCHSA GQWFAVEECM TGEFRKYNNN NGDEIIPTNT LEEIMLAFSH
1760 1770 1780 1790 1800
WTYEYTRGEL LVLDLQGVGE NLTDPSVIKA EEKRSCDMVF GPANLGEDAI
1810 1820 1830 1840 1850
KNFRAKHHCN SCCRKLKLPD LKRNDYTPDK IIFPQDESSD LNLQAGNSTK
1860
ESEATNSVRL ML
Note: No experimental confirmation available. Gene prediction based on similarity to murine ortholog.
Length:1,862
Mass (Da):212,376
Last modified:November 25, 2008 - v2
Checksum:i25C529CCAA3DC429
GO
Isoform 21 Publication (identifier: Q925B3-2) [UniParc]FASTAAdd to basket
Also known as: PLIK1 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     1-1515: Missing.
     1516-1536: PDWLRDRPTNREMPSEGGTLN → MSELKFILHGCFLKSDLFTIL

Show »
Length:347
Mass (Da):39,616
Checksum:i8AC64144872AD0B6
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0529831 – 1515Missing in isoform 2. 1 PublicationAdd BLAST1515
Alternative sequenceiVSP_0529841516 – 1536PDWLR…GGTLN → MSELKFILHGCFLKSDLFTI L in isoform 2. 1 PublicationAdd BLAST21

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF375874 mRNA. Translation: AAK54810.1.
AABR03025009 Genomic DNA. No translation available.
AABR03026190 Genomic DNA. No translation available.
AABR03028037 Genomic DNA. No translation available.
AABR03028619 Genomic DNA. No translation available.
AABR03029036 Genomic DNA. No translation available.
AABR03029588 Genomic DNA. No translation available.
AABR03030279 Genomic DNA. No translation available.
AABR03031904 Genomic DNA. No translation available.
EF673694 mRNA. Translation: ABS12242.1.
UniGeneiRn.233566.
Rn.86991.

Genome annotation databases

UCSCiRGD:620053. rat. [Q925B3-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF375874 mRNA. Translation: AAK54810.1.
AABR03025009 Genomic DNA. No translation available.
AABR03026190 Genomic DNA. No translation available.
AABR03028037 Genomic DNA. No translation available.
AABR03028619 Genomic DNA. No translation available.
AABR03029036 Genomic DNA. No translation available.
AABR03029588 Genomic DNA. No translation available.
AABR03030279 Genomic DNA. No translation available.
AABR03031904 Genomic DNA. No translation available.
EF673694 mRNA. Translation: ABS12242.1.
UniGeneiRn.233566.
Rn.86991.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3E7KX-ray2.01A/B/C/D/E/F/G/H1198-1249[»]
SMRiQ925B3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ925B3. 1 interactor.
MINTiMINT-2778884.
STRINGi10116.ENSRNOP00000034169.

Chemistry databases

GuidetoPHARMACOLOGYi499.

PTM databases

iPTMnetiQ925B3.
PhosphoSitePlusiQ925B3.
SwissPalmiQ925B3.

Proteomic databases

PaxDbiQ925B3.
PRIDEiQ925B3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiRGD:620053. rat. [Q925B3-1]

Organism-specific databases

RGDi620053. Trpm7.

Phylogenomic databases

eggNOGiKOG3614. Eukaryota.
ENOG410XR5B. LUCA.
HOGENOMiHOG000230920.
HOVERGENiHBG055663.
InParanoidiQ925B3.
PhylomeDBiQ925B3.

Miscellaneous databases

EvolutionaryTraceiQ925B3.
PROiQ925B3.

Family and domain databases

InterProiIPR005821. Ion_trans_dom.
IPR011009. Kinase-like_dom.
IPR004166. MHCK_EF2_kinase.
IPR029601. TRPM7.
IPR032415. TRPM_tetra.
[Graphical view]
PANTHERiPTHR13800:SF8. PTHR13800:SF8. 3 hits.
PfamiPF02816. Alpha_kinase. 1 hit.
PF00520. Ion_trans. 1 hit.
PF16519. TRPM_tetra. 1 hit.
[Graphical view]
SMARTiSM00811. Alpha_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS51158. ALPHA_KINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRPM7_RAT
AccessioniPrimary (citable) accession number: Q925B3
Secondary accession number(s): A7L642
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 25, 2008
Last sequence update: November 25, 2008
Last modified: November 2, 2016
This is version 103 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.