Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

PRKC apoptosis WT1 regulator protein

Gene

Pawr

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Pro-apoptopic protein capable of selectively inducing apoptosis in cancer cells, sensitizing the cells to diverse apoptotic stimuli and causing regression of tumors in animal models. Induces apoptosis in certain cancer cells by activation of the Fas prodeath pathway and coparallel inhibition of NF-kappa-B transcriptional activity. Inhibits the transcriptional activation and augments the transcriptional repression mediated by WT1. Down-regulates the anti-apoptotic protein BCL2 via its interaction with WT1. Seems also to be a transcriptional repressor by itself. May be directly involved in regulating the amyloid precursor protein (APP) cleavage activity of BACE1 (By similarity).By similarity1 Publication

Miscellaneous

The synapses are crucial cellular sites for the cell death promoting actions of PAWR in motor neurons. Targeted inhibition of PAWR by RNAi is neuroprotective.

GO - Molecular functioni

GO - Biological processi

  • apoptotic signaling pathway Source: MGI
  • interleukin-2 biosynthetic process Source: MGI
  • negative regulation of B cell proliferation Source: MGI
  • negative regulation of epithelial to mesenchymal transition Source: BHF-UCL
  • negative regulation of fibroblast proliferation Source: BHF-UCL
  • negative regulation of gene expression Source: BHF-UCL
  • negative regulation of T cell proliferation Source: MGI
  • negative regulation of T cell receptor signaling pathway Source: MGI
  • negative regulation of transcription by RNA polymerase II Source: BHF-UCL
  • positive regulation of amyloid precursor protein biosynthetic process Source: MGI
  • positive regulation of apoptotic process Source: GO_Central
  • positive regulation of cellular senescence Source: BHF-UCL
  • positive regulation of gene expression Source: BHF-UCL
  • positive regulation of hydrogen peroxide-mediated programmed cell death Source: BHF-UCL
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Biological processApoptosis, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
PRKC apoptosis WT1 regulator protein
Alternative name(s):
Prostate apoptosis response 4 protein
Short name:
Par-4
Gene namesi
Name:Pawr
Synonyms:Par4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:2149961 Pawr

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000582371 – 333PRKC apoptosis WT1 regulator proteinAdd BLAST333

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei156Phosphothreonine; by PKABy similarity1
Modified residuei224PhosphoserineBy similarity1

Post-translational modificationi

Preferentially phosphorylated at the Thr-156 by PKC in cancer cells.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ925B0
PeptideAtlasiQ925B0
PRIDEiQ925B0

PTM databases

iPTMnetiQ925B0
PhosphoSitePlusiQ925B0

Expressioni

Gene expression databases

BgeeiENSMUSG00000035873
CleanExiMM_PAWR
GenevisibleiQ925B0 MM

Interactioni

Subunit structurei

Homooligomer. Interacts (via the C-terminal region) with WT1. Interacts with THAP1. Interacts with AATF. Interacts with BACE1. Interacts with SPSB1 (via B30.2/SPRY domain); this interaction is direct and occurs in association with the Elongin BC complex (PubMed:16369487, PubMed:20561531). Interacts with SPSB2 (via B30.2/SPRY domain); this interaction occurs in association with the Elongin BC complex (PubMed:16369487, PubMed:20561531). Interacts with SPSB4 (via B30.2/SPRY domain); this interaction occurs in association with the Elongin BC complex (PubMed:16369487, PubMed:20561531). Component of a ternary complex composed of SQSTM1 and PRKCZ (By similarity). Interacts with actin (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Dapk3O547842EBI-77397,EBI-77359

GO - Molecular functioni

Protein-protein interaction databases

BioGridi2278705 interactors.
IntActiQ925B0 1 interactor.
STRINGi10090.ENSMUSP00000092951

Structurei

3D structure databases

ProteinModelPortaliQ925B0
SMRiQ925B0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni138 – 196Selective for apoptosis induction in cancer cells (SAC)Add BLAST59
Regioni293 – 333Leucine-zipperAdd BLAST41

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili255 – 333Sequence analysisAdd BLAST79

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi62 – 66B30.2/SPRY domain-binding motifBy similarity5
Motifi138 – 154Nuclear localization signalBy similarityAdd BLAST17

Domaini

The leucine-zipper domain is not essential for apoptosis, but is required for sensitization of cells to exogenous apoptotic insults and for interaction with its partners.By similarity
The SAC domain is a death-inducing domain selective for apoptosis induction in cancer cells. This domain is essential for nuclear entry, Fas activation, inhibition of NF-kappa-B activity and induction of apoptosis in cancer cells (By similarity).By similarity
The B30.2/SPRY domain-binding motif mediates recognition by proteins containing a B30.2/SPRY domain.By similarity

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IFBP Eukaryota
ENOG4111M3H LUCA
GeneTreeiENSGT00390000000406
HOGENOMiHOG000115462
HOVERGENiHBG058812
InParanoidiQ925B0
OMAiNCAVGPA
OrthoDBiEOG091G0JYK
PhylomeDBiQ925B0
TreeFamiTF332824

Family and domain databases

InterProiView protein in InterPro
IPR026117 Par-4
PANTHERiPTHR15093 PTHR15093, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q925B0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MATGGYRSGG STTTDFLEEW KAKREKMRAK QNPAGPGSSG GDPAAKSPAG
60 70 80 90 100
SLTPTAVAGT SELNHGPAGA AAPAAPAPGA LNCAHGSSTL PRAAPGSRRA
110 120 130 140 150
EDECPSAAAA SGAPGSRGDE EEPDSAREKG RSSGPSARKG KGQIEKRKLR
160 170 180 190 200
EKRRSTGVVN IPAAECLDEY EDDEAGQKER KREDAITQQN TIQNEAATLP
210 220 230 240 250
DPGTSYLPQD PSRTVPGRYK STTSAPEDEI SNRYPRTDRS GFSRHNRDAN
260 270 280 290 300
APASFSSSST LEKRIEDLEK EVVRERQENL RLVRLMQDKE EMIGKLKEEI
310 320 330
DLLNRDLDDM EDENEQLKQE NKTLLKVVGQ LTR
Length:333
Mass (Da):35,908
Last modified:December 12, 2006 - v2
Checksum:i80CD3DBA586C01F7
GO
Isoform 2 (identifier: Q925B0-2) [UniParc]FASTAAdd to basket
Also known as: P33

The sequence of this isoform differs from the canonical sequence as follows:
     166-209: Missing.

Show »
Length:289
Mass (Da):30,944
Checksum:i3B729F993F35745F
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_022018166 – 209Missing in isoform 2. 1 PublicationAdd BLAST44

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ449073 mRNA Translation: ABE27591.1
DQ449074 mRNA Translation: ABE27592.1
DQ363525 mRNA Translation: ABC96645.1
AF377871 mRNA Translation: AAK55414.1
CCDSiCCDS36053.1 [Q925B0-1]
RefSeqiNP_473397.1, NM_054056.2 [Q925B0-1]
UniGeneiMm.391419

Genome annotation databases

EnsembliENSMUST00000095313; ENSMUSP00000092951; ENSMUSG00000035873 [Q925B0-1]
ENSMUST00000218332; ENSMUSP00000151457; ENSMUSG00000035873 [Q925B0-2]
GeneIDi114774
KEGGimmu:114774
UCSCiuc007gzf.1 mouse [Q925B0-1]
uc011xnd.1 mouse [Q925B0-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiPAWR_MOUSE
AccessioniPrimary (citable) accession number: Q925B0
Secondary accession number(s): Q0ZHI4, Q2HYJ1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 24, 2005
Last sequence update: December 12, 2006
Last modified: March 28, 2018
This is version 118 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome