Reviewed, UniProtKB/Swiss-Prot Q92598 (HS105_HUMAN)

Names and origin

Protein names

Recommended name:
    Heat shock protein 105 kDa
Alternative name(s):
    Heat shock 110 kDa protein
    Antigen NY-CO-25

Gene names

Name:	HSPH1
Synonyms:	HSP105, HSP110, KIAA0201

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	858 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Prevents the aggregation of denatured proteins in cells under severe stress, on which the ATP levels decrease markedly. Inhibits HSPA8/HSC70 ATPase and chaperone activities By similarity.
Subunit structure	Interacts with HSPA8/HSC70 By similarity.
Subcellular location	Cytoplasm. Ref.1
Tissue specificity	Highly expressed in testis. Present at lower levels in most brain regions, except cerebellum. Overexpressed in cancer cells. Ref.8 Ref.9
Post-translational modification	Phosphorylation on Ser-509 may be important for regulation of the HSPA8/HSC70 chaperone activity By similarity.
Sequence similarities	Belongs to the heat shock protein 70 family.

Ontologies

Keywords
Biological process	Stress response
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Nucleotide-binding
PTM	Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	response to unfolded protein Ref.1 Traceable author statement. Source: ProtInc
Cellular component	cytoplasm Ref.1 Traceable author statement. Source: ProtInc
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform Alpha (identifier: Q92598-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Beta (identifier: Q92598-2)

The sequence of this isoform differs from the canonical sequence as follows:
     529-572: Missing.

Isoform 3 (identifier: Q92598-3)

The sequence of this isoform differs from the canonical sequence as follows:
     104-144: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 858	858	Heat shock protein 105 kDa		PRO_0000078284
Amino acid modifications
	Modified residue	31	1	Phosphoserine Ref.15
	Modified residue	384	1	Phosphoserine Ref.12
	Modified residue	509	1	Phosphoserine By similarity
	Modified residue	557	1	Phosphoserine Ref.14
	Modified residue	700	1	Phosphothreonine Ref.16
	Modified residue	809	1	Phosphoserine Ref.15 Ref.14 Ref.16 Ref.10 Ref.11 Ref.13
	Modified residue	815	1	Phosphothreonine Ref.12 Ref.16
Natural variations
	Alternative sequence	104 – 144	41	Missing in isoform 3.		VSP_035428
	Alternative sequence	529 – 572	44	Missing in isoform Beta.		VSP_002428

Sequences

	Sequence		Length	Mass (Da)
	Isoform Alpha [UniParc]. Last modified February 1, 1997. Version 1. Checksum: D0E757970E340B56	FASTA	858	96,865
	10 20 30 40 50 60 MSVVGLDVGS QSCYIAVARA GGIETIANEF SDRCTPSVIS FGSKNRTIGV AAKNQQITHA 70 80 90 100 110 120 NNTVSNFKRF HGRAFNDPFI QKEKENLSYD LVPLKNGGVG IKVMYMGEEH LFSVEQITAM 130 140 150 160 170 180 LLTKLKETAE NSLKKPVTDC VISVPSFFTD AERRSVLDAA QIVGLNCLRL MNDMTAVALN 190 200 210 220 230 240 YGIYKQDLPS LDEKPRIVVF VDMGHSAFQV SACAFNKGKL KVLGTAFDPF LGGKNFDEKL 250 260 270 280 290 300 VEHFCAEFKT KYKLDAKSKI RALLRLYQEC EKLKKLMSSN STDLPLNIEC FMNDKDVSGK 310 320 330 340 350 360 MNRSQFEELC AELLQKIEVP LYSLLEQTHL KVEDVSAVEI VGGATRIPAV KERIAKFFGK 370 380 390 400 410 420 DISTTLNADE AVARGCALQC AILSPAFKVR EFSVTDAVPF PISLIWNHDS EDTEGVHEVF 430 440 450 460 470 480 SRNHAAPFSK VLTFLRRGPF ELEAFYSDPQ GVPYPEAKIG RFVVQNVSAQ KDGEKSRVKV 490 500 510 520 530 540 KVRVNTHGIF TISTASMVEK VPTEENEMSS EADMECLNQR PPENPDTDKN VQQDNSEAGT 550 560 570 580 590 600 QPQVQTDAQQ TSQSPPSPEL TSEENKIPDA DKANEKKVDQ PPEAKKPKIK VVNVELPIEA 610 620 630 640 650 660 NLVWQLGKDL LNMYIETEGK MIMQDKLEKE RNDAKNAVEE YVYEFRDKLC GPYEKFICEQ 670 680 690 700 710 720 DHQNFLRLLT ETEDWLYEEG EDQAKQAYVD KLEELMKIGT PVKVRFQEAE ERPKMFEELG 730 740 750 760 770 780 QRLQHYAKIA ADFRNKDEKY NHIDESEMKK VEKSVNEVME WMNNVMNAQA KKSLDQDPVV 790 800 810 820 830 840 RAQEIKTKIK ELNNTCEPVV TQPKPKIESP KLERTPNGPN IDKKEEDLED KNNFGAEPPH 850 QNGECYPNEK NSVNMDLD
	Isoform Beta. Checksum: 10272B4D373289E8	FASTA	814	92,116
	10 20 30 40 50 60 MSVVGLDVGS QSCYIAVARA GGIETIANEF SDRCTPSVIS FGSKNRTIGV AAKNQQITHA 70 80 90 100 110 120 NNTVSNFKRF HGRAFNDPFI QKEKENLSYD LVPLKNGGVG IKVMYMGEEH LFSVEQITAM 130 140 150 160 170 180 LLTKLKETAE NSLKKPVTDC VISVPSFFTD AERRSVLDAA QIVGLNCLRL MNDMTAVALN 190 200 210 220 230 240 YGIYKQDLPS LDEKPRIVVF VDMGHSAFQV SACAFNKGKL KVLGTAFDPF LGGKNFDEKL 250 260 270 280 290 300 VEHFCAEFKT KYKLDAKSKI RALLRLYQEC EKLKKLMSSN STDLPLNIEC FMNDKDVSGK 310 320 330 340 350 360 MNRSQFEELC AELLQKIEVP LYSLLEQTHL KVEDVSAVEI VGGATRIPAV KERIAKFFGK 370 380 390 400 410 420 DISTTLNADE AVARGCALQC AILSPAFKVR EFSVTDAVPF PISLIWNHDS EDTEGVHEVF 430 440 450 460 470 480 SRNHAAPFSK VLTFLRRGPF ELEAFYSDPQ GVPYPEAKIG RFVVQNVSAQ KDGEKSRVKV 490 500 510 520 530 540 KVRVNTHGIF TISTASMVEK VPTEENEMSS EADMECLNQR PPENPDTDAN EKKVDQPPEA 550 560 570 580 590 600 KKPKIKVVNV ELPIEANLVW QLGKDLLNMY IETEGKMIMQ DKLEKERNDA KNAVEEYVYE 610 620 630 640 650 660 FRDKLCGPYE KFICEQDHQN FLRLLTETED WLYEEGEDQA KQAYVDKLEE LMKIGTPVKV 670 680 690 700 710 720 RFQEAEERPK MFEELGQRLQ HYAKIAADFR NKDEKYNHID ESEMKKVEKS VNEVMEWMNN 730 740 750 760 770 780 VMNAQAKKSL DQDPVVRAQE IKTKIKELNN TCEPVVTQPK PKIESPKLER TPNGPNIDKK 790 800 810 EEDLEDKNNF GAEPPHQNGE CYPNEKNSVN MDLD
	Isoform 3. Checksum: 19C55A76242953F2	FASTA	817	92,255
	10 20 30 40 50 60 MSVVGLDVGS QSCYIAVARA GGIETIANEF SDRCTPSVIS FGSKNRTIGV AAKNQQITHA 70 80 90 100 110 120 NNTVSNFKRF HGRAFNDPFI QKEKENLSYD LVPLKNGGVG IKVPSFFTDA ERRSVLDAAQ 130 140 150 160 170 180 IVGLNCLRLM NDMTAVALNY GIYKQDLPSL DEKPRIVVFV DMGHSAFQVS ACAFNKGKLK 190 200 210 220 230 240 VLGTAFDPFL GGKNFDEKLV EHFCAEFKTK YKLDAKSKIR ALLRLYQECE KLKKLMSSNS 250 260 270 280 290 300 TDLPLNIECF MNDKDVSGKM NRSQFEELCA ELLQKIEVPL YSLLEQTHLK VEDVSAVEIV 310 320 330 340 350 360 GGATRIPAVK ERIAKFFGKD ISTTLNADEA VARGCALQCA ILSPAFKVRE FSVTDAVPFP 370 380 390 400 410 420 ISLIWNHDSE DTEGVHEVFS RNHAAPFSKV LTFLRRGPFE LEAFYSDPQG VPYPEAKIGR 430 440 450 460 470 480 FVVQNVSAQK DGEKSRVKVK VRVNTHGIFT ISTASMVEKV PTEENEMSSE ADMECLNQRP 490 500 510 520 530 540 PENPDTDKNV QQDNSEAGTQ PQVQTDAQQT SQSPPSPELT SEENKIPDAD KANEKKVDQP 550 560 570 580 590 600 PEAKKPKIKV VNVELPIEAN LVWQLGKDLL NMYIETEGKM IMQDKLEKER NDAKNAVEEY 610 620 630 640 650 660 VYEFRDKLCG PYEKFICEQD HQNFLRLLTE TEDWLYEEGE DQAKQAYVDK LEELMKIGTP 670 680 690 700 710 720 VKVRFQEAEE RPKMFEELGQ RLQHYAKIAA DFRNKDEKYN HIDESEMKKV EKSVNEVMEW 730 740 750 760 770 780 MNNVMNAQAK KSLDQDPVVR AQEIKTKIKE LNNTCEPVVT QPKPKIESPK LERTPNGPNI 790 800 810 DKKEEDLEDK NNFGAEPPHQ NGECYPNEKN SVNMDLD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning, expression and localization of human 105 kDa heat shock protein, hsp105." Ishihara K., Yasuda K., Hatayama T. Biochim. Biophys. Acta 1444:138-142(1999) [PubMed: 9931472] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS ALPHA AND BETA), SUBCELLULAR LOCATION.
[2]	"Characterization of human colon cancer antigens recognized by autologous antibodies." Scanlan M.J., Chen Y.-T., Williamson B., Gure A.O., Stockert E., Gordan J.D., Tuereci O., Sahin U., Pfreundschuh M., Old L.J. Int. J. Cancer 76:652-658(1998) [PubMed: 9610721] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM ALPHA). Tissue: Colon carcinoma.
[3]	"Prediction of the coding sequences of unidentified human genes. VI. The coding sequences of 80 new genes (KIAA0201-KIAA0280) deduced by analysis of cDNA clones from cell line KG-1 and brain." Nagase T., Seki N., Ishikawa K., Ohira M., Kawarabayasi Y., Ohara O., Tanaka A., Kotani H., Miyajima N., Nomura N. DNA Res. 3:321-329(1996) [PubMed: 9039502] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM ALPHA). Tissue: Bone marrow.
[4]	"The DNA sequence and analysis of human chromosome 13." Dunham A., Matthews L.H., Burton J., Ashurst J.L., Howe K.L., Ashcroft K.J., Beare D.M., Burford D.C., Hunt S.E., Griffiths-Jones S., Jones M.C., Keenan S.J., Oliver K., Scott C.E., Ainscough R., Almeida J.P., Ambrose K.D., Andrews D.T. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Bannerjee R., Barlow K.F., Bates K., Beasley H., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burrill W., Carder C., Carter N.P., Chapman J.C., Clamp M.E., Clark S.Y., Clarke G., Clee C.M., Clegg S.C., Cobley V., Collins J.E., Corby N., Coville G.J., Deloukas P., Dhami P., Dunham I., Dunn M., Earthrowl M.E., Ellington A.G., Faulkner L., Frankish A.G., Frankland J., French L., Garner P., Garnett J., Gilbert J.G.R., Gilson C.J., Ghori J., Grafham D.V., Gribble S.M., Griffiths C., Hall R.E., Hammond S., Harley J.L., Hart E.A., Heath P.D., Howden P.J., Huckle E.J., Hunt P.J., Hunt A.R., Johnson C., Johnson D., Kay M., Kimberley A.M., King A., Laird G.K., Langford C.J., Lawlor S., Leongamornlert D.A., Lloyd D.M., Lloyd C., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., McLaren S.J., McMurray A., Milne S., Moore M.J.F., Nickerson T., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K.M., Rice C.M., Searle S., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Steward C.A., Sycamore N., Tester J., Thomas D.W., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Wilming L., Wray P.W., Wright M.W., Young L., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Beck S., Bentley D.R., Rogers J., Ross M.T. Nature 428:522-528(2004) [PubMed: 15057823] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM ALPHA). Tissue: Testis.
[7]	Lubec G., Chen W.-Q., Sun Y. Submitted (DEC-2008) to UniProtKB Cited for: PROTEIN SEQUENCE OF 332-346 AND 375-388, MASS SPECTROMETRY. Tissue: Fetal brain cortex.
[8]	"The distribution and localization of hsp110 in brain." Hylander B.L., Chen X., Graf P.C.F., Subjeck J.R. Brain Res. 869:49-55(2000) [PubMed: 10865058] [Abstract] Cited for: TISSUE SPECIFICITY.
[9]	"DNA vaccination of HSP105 leads to tumor rejection of colorectal cancer and melanoma in mice through activation of both CD4 T cells and CD8 T cells." Miyazaki M., Nakatsura T., Yokomine K., Senju S., Monji M., Hosaka S., Komori H., Yoshitake Y., Motomura Y., Minohara M., Kubo T., Ishihara K., Hatayama T., Ogawa M., Nishimura Y. Cancer Sci. 96:695-705(2005) [PubMed: 16232202] [Abstract] Cited for: TISSUE SPECIFICITY.
[10]	"Global phosphoproteome of HT-29 human colon adenocarcinoma cells." Kim J.-E., Tannenbaum S.R., White F.M. J. Proteome Res. 4:1339-1346(2005) [PubMed: 16083285] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-809, MASS SPECTROMETRY.
[11]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-809, MASS SPECTROMETRY. Tissue: Epithelium.
[12]	"A probability-based approach for high-throughput protein phosphorylation analysis and site localization." Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P. Nat. Biotechnol. 24:1285-1292(2006) [PubMed: 16964243] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-384 AND THR-815, MASS SPECTROMETRY. Tissue: Epithelium.
[13]	"Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra." Yu L.-R., Zhu Z., Chan K.C., Issaq H.J., Dimitrov D.S., Veenstra T.D. J. Proteome Res. 6:4150-4162(2007) [PubMed: 17924679] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-809, MASS SPECTROMETRY. Tissue: Epithelium.
[14]	"Global proteomic profiling of phosphopeptides using electron transfer dissociation tandem mass spectrometry." Molina H., Horn D.M., Tang N., Mathivanan S., Pandey A. Proc. Natl. Acad. Sci. U.S.A. 104:2199-2204(2007) [PubMed: 17287340] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-557 AND SER-809, MASS SPECTROMETRY.
[15]	"Automated phosphoproteome analysis for cultured cancer cells by two-dimensional nanoLC-MS using a calcined titania/C18 biphasic column." Imami K., Sugiyama N., Kyono Y., Tomita M., Ishihama Y. Anal. Sci. 24:161-166(2008) [PubMed: 18187866] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-31 AND SER-809, MASS SPECTROMETRY.
[16]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-700; SER-809 AND THR-815, MASS SPECTROMETRY.
[17]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
+	Additional computationally mapped references.

Web resources

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB003333 mRNA. Translation: BAA34779.1. AB003334 mRNA. Translation: BAA34780.1. AF039695 mRNA. Translation: AAC18044.1. Different initiation. D86956 mRNA. Translation: BAA13192.2. Different initiation. AL137142 Genomic DNA. Translation: CAI12428.1. AL137142 Genomic DNA. Translation: CAI12429.1. AL137142 Genomic DNA. Translation: CAI12430.1. CH471075 Genomic DNA. Translation: EAX08482.1. BC037553 mRNA. Translation: AAH37553.1.
IPI	IPI00218993. IPI00513743. IPI00514983.
RefSeq	NP_006635.2.
UniGene	Hs.36927
3D structure databases
HSSP	HSSP built from PDB template 1BUP based on UniProtKB P19120.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q92598. 5 interactions.
PTM databases
PhosphoSite	Q92598.
2-D gel databases
REPRODUCTION-2DPAGE	Q92598.
Proteomic databases
PRIDE	Q92598.
Genome annotation databases
Ensembl	ENSG00000120694. Homo sapiens. [Contig view]
GeneID	10808.
KEGG	hsa:10808.
Organism-specific databases
GeneCards	GC13M030608.
H-InvDB	HIX0011215.
HGNC	HGNC:16969. HSPH1.
HPA	CAB002060.
MIM	610703. gene.
PharmGKB	PA134869917.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q92598.
HOVERGEN	Q92598.
OMA	Q92598. LMEQTQL.
Gene expression databases
ArrayExpress	Q92598.
Bgee	Q92598.
CleanEx	HS_HSPH1.
GermOnline	ENSG00000120694. Homo sapiens.
Family and domain databases
InterPro	IPR018181. Heat_shock_70_CS. IPR001023. Hsp70. IPR013126. Hsp_70. [Graphical view]
PANTHER	PTHR19375. Hsp70. 1 hit.
Pfam	PF00012. HSP70. 1 hit. [Graphical view]
PRINTS	PR00301. HEATSHOCK70.
ProDom	PD000089. Hsp70. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00297. HSP70_1. False negative. PS00329. HSP70_2. False negative. PS01036. HSP70_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	41059.
PMAP-CutDB	Q92598.
SOURCE	Search...

Entry information

Entry name

HS105_HUMAN

Accession

Primary (citable) accession number: Q92598
Secondary accession number(s): O95739 , Q5TBM6, Q5TBM7, Q5TBM8, Q9UPC4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	June 16, 2009
This is version 100 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)