Skip Header

 
Contribute Send feedback

Reviewed, UniProtKB/Swiss-Prot Q92597 (NDRG1_HUMAN)

Last modified November 25, 2008. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Web resources · Cross-references · Entry information · Relevant documents

Hide | Top

Names and origin

Protein namesRecommended name:
    Protein NDRG1
Alternative name(s):
    N-myc downstream-regulated gene 1 protein
    Differentiation-related gene 1 protein
      Short name=DRG-1
    Reducing agents and tunicamycin-responsive protein
      Short name=RTP
    Nickel-specific induction protein Cap43
    Rit42
Gene names
Name: NDRG1
Synonyms: CAP43, DRG1, RTP
OrganismHomo sapiens (Human)
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Hide | Top

Protein attributes

Sequence length394 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

Hide | Top

General annotation (Comments)

Function

May have a growth inhibitory role.

Subcellular location

Cytoplasm. Nucleus. Cell membrane. Note= Whereas in prostate epithelium and placental chorion it is located in both the cytoplasm and the nucleus, nuclear staining is not observed in colon epithelium cells. Instead its localization changes from the cytoplasm to the plasma membrane during differentiation of colon carcinoma cell lines in vitro.

Tissue specificity

Ubiquitous; expressed most prominently in placental membranes and prostate, kidney, small intestine, and ovary tissues. Reduced expression in adenocarcinomas compared to normal tissues. In colon, prostate and placental membranes, the cells that border the lumen show the highest expression.

Induction

By homocysteine, 2-mercaptoethanol, tunicamycin in endothelial cells. Induced approximately 20-fold during in vitro differentiation of the colon carcinoma cell lines HT29-D4 and Caco-2. Induced by nickel compounds in all tested cell lines. The primary signal for its induction is an elevation of free intracellular calcium ion caused by nickel ion exposure. Okadaic acid, a serine/threonine phosphatase inhibitor, induced its expression more rapidly and more efficiently than nickel.

Involvement in disease

Defects in NDRG1 are the cause of Charcot-Marie-Tooth disease type 4D (CMT4D) [MIM:601455]; also known as hereditary motor and sensory neuropathy Lom type (HMSNL). CMT4D is a recessive form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy and primary peripheral axonal neuropathy. Demyelinating CMT neuropathies are characterized by severely reduced nerve conduction velocities (less than 38 m/sec), segmental demyelination and remyelination with onion bulb formations on nerve biopsy, slowly progressive distal muscle atrophy and weakness, absent deep tendon reflexes, and hollow feet. By convention, autosomal recessive forms of demyelinating Charcot-Marie-Tooth disease are designated CMT4.

Sequence similarities

Belongs to the NDRG family.

Hide | Top

Ontologies

Keywords

   Cellular componentCell membrane
Cytoplasm
Membrane
Nucleus
   DiseaseCharcot-Marie-Tooth disease
   DomainRepeat
   PTMPhosphoprotein

Gene Ontology (GO)

   Biological processresponse to metal ion Ref.3

Traceable author statement. Source: ProtInc

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-KW

cytoskeleton

Inferred from direct assay. Source: HPA

nucleus

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionprotein binding

Inferred from physical interaction. Source: IntAct

Complete GO annotation...

Hide | Top

Binary interactions

With

Entry

#Exp.

IntAct

Notes

ACSL3O955731EBI-716486,EBI-1190822
ACTG1P632611EBI-716486,EBI-351292
AP1M2Q9Y6Q5-11EBI-716486,EBI-752259
AP2M1Q96CW11EBI-716486,EBI-297683
ATP1A1P050231EBI-716486,EBI-358778
CANXP278242EBI-716486,EBI-355947
CLTCQ006101EBI-716486,EBI-354967
CNDP2Q96KP41EBI-716486,EBI-1190734
COPB2P356061EBI-716486,EBI-1056534
CTNNB1P35222-11EBI-716486,EBI-491563
DARSP148681EBI-716486,EBI-358730
DDX1Q924991EBI-716486,EBI-358474
DDX5P178441EBI-716486,EBI-351962
DLSTP369571EBI-716486,EBI-351007
EEF1GP266411EBI-716486,EBI-351467
EEF2P136391EBI-716486,EBI-352560
EIF2S3P410911EBI-716486,EBI-1054228
EIF3EP602281EBI-716486,EBI-347740
EWSR1Q018441EBI-716486,EBI-739737
FASNP493271EBI-716486,EBI-356658
HNRNPFP525971EBI-716486,EBI-352986
HNRNPH1P319431EBI-716486,EBI-351590
HNRNPUQ008391EBI-716486,EBI-351126
HSD17B4P516591EBI-716486,EBI-358398
HSP90AA1P079001EBI-716486,EBI-296047
HSPA5P110211EBI-716486,EBI-354921
ILF3Q12906-51EBI-716486,EBI-288405
KIF5BP331761EBI-716486,EBI-355878
LDHAP00338-11EBI-716486,EBI-681495
MAOAP213971EBI-716486,EBI-1190845
MMEP084731EBI-716486,EBI-353759
NCLP193381EBI-716486,EBI-352553
PABPC1P119401EBI-716486,EBI-81531
PKM2P146181EBI-716486,EBI-353408
PPP2R2AP631511EBI-716486,EBI-1048931
PSMC2P359981EBI-716486,EBI-359710
PSMC3P179801EBI-716486,EBI-359720
PSMD2Q132001EBI-716486,EBI-357648
RPL24P837311EBI-716486,EBI-353146
RPL3P390231EBI-716486,EBI-1056348
RPL4P365781EBI-716486,EBI-348313
RPN2P048441EBI-716486,EBI-719731
RPS16P622491EBI-716486,EBI-352480
RPS20P608661EBI-716486,EBI-353105
RPS26P628541EBI-716486,EBI-353438
RPS3P233961EBI-716486,EBI-351193
RPS6P627531EBI-716486,EBI-356625
RPS8P622411EBI-716486,EBI-351811
RPS9P467811EBI-716486,EBI-351206
RUVBL2Q9Y2301EBI-716486,EBI-352939
SEC23AQ154361EBI-716486,EBI-81088
SHMT2P348971EBI-716486,EBI-352908
SLC25A6P122361EBI-716486,EBI-356254
TARSP266391EBI-716486,EBI-1042683
TLE3Q04726-11EBI-716486,EBI-1052074
UPF1Q929001EBI-716486,EBI-373471
VCPP550721EBI-716486,EBI-355164
XRCC6P129562EBI-716486,EBI-353208

Hide | Top

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 394394Protein NDRG1
PRO_0000159573

Regions

Repeat339 – 348101
Repeat349 – 358102
Repeat359 – 368103
Region339 – 368303 X 10 AA tandem repeats of G-T-R-S-R-S-H-T-S-E

Amino acid modifications

Modified residue3191Phosphoserine
Modified residue3261Phosphoserine
Modified residue3281Phosphothreonine
Modified residue3301Phosphoserine
Modified residue3321Phosphoserine
Modified residue3331Phosphoserine
Modified residue3351Phosphothreonine
Modified residue3361Phosphoserine
Modified residue3641Phosphoserine
Modified residue3661Phosphothreonine
Modified residue3751Phosphothreonine

Experimental info

Sequence conflict1451I → T in CAA63430. Ref.2

Hide | Top

Sequences

Sequence LengthMass (Da)Tools
Q92597-1 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: 4C816B9C85E3756F

FASTA39442,835
        10         20         30         40         50         60 
MSREMQDVDL AEVKPLVEKG ETITGLLQEF DVQEQDIETL HGSVHVTLCG TPKGNRPVIL 

        70         80         90        100        110        120 
TYHDIGMNHK TCYNPLFNYE DMQEITQHFA VCHVDAPGQQ DGAASFPAGY MYPSMDQLAE 

       130        140        150        160        170        180 
MLPGVLQQFG LKSIIGMGTG AGAYILTRFA LNNPEMVEGL VLINVNPCAE GWMDWAASKI 

       190        200        210        220        230        240 
SGWTQALPDM VVSHLFGKEE MQSNVEVVHT YRQHIVNDMN PGNLHLFINA YNSRRDLEIE 

       250        260        270        280        290        300 
RPMPGTHTVT LQCPALLVVG DSSPAVDAVV ECNSKLDPTK TTLLKMADCG GLPQISQPAK 

       310        320        330        340        350        360 
LAEAFKYFVQ GMGYMPSASM TRLMRSRTAS GSSVTSLDGT RSRSHTSEGT RSRSHTSEGT 

       370        380        390 
RSRSHTSEGA HLDITPNSGA AGNSAGPKSM EVSC

« Hide

Hide | Top

References

« Hide 'large scale' references
[1]"Homocysteine-respondent genes in vascular endothelial cells identified by differential display analysis. GRP78/BiP and novel genes."
Kokame K., Kato H., Miyata T.
J. Biol. Chem. 271:29659-29665(1996) [PubMed: 8939898] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Umbilical vein endothelial cell.
[2]"A novel gene which is up-regulated during colon epithelial cell differentiation and down-regulated in colorectal neoplasms."
van Belzen N., Dinjens W.N.M., Diesveld M.P.G., Groen N.A., van der Made A.C.J., Nozawa Y., Vlietstra R., Trapman J., Bosman F.T.
Lab. Invest. 77:85-92(1997) [PubMed: 9251681] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Cap43, a novel gene specifically induced by Ni2+ compounds."
Zhou D., Salnikow K., Costa M.
Cancer Res. 58:2182-2189(1998) [PubMed: 9605764] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Lung.
[4]"Differential expression of the RTP/Drg1/Ndr1 gene product in proliferating and growth arrested cells."
Piquemal D., Joulia D., Balaguer P., Basset A., Marti J., Commes T.
Biochim. Biophys. Acta 1450:364-373(1999) [PubMed: 10395947] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[5]"DNA sequence and analysis of human chromosome 8."
Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M., Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S., Asakawa T. expand/collapse author list , Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A., Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III, Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K., Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P., Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H., Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B., O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K., Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L., Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G., Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W., Platzer M., Shimizu N., Lander E.S.
Nature 439:331-335(2006) [PubMed: 16421571] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Kidney.
[7]Angelicheva D., Kalaydjieva L.
Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-21.
Tissue: Brain.
[8]"Inhibition of tumor cell growth by RTP/rit42 and its responsiveness to p53 and DNA damage."
Kurdistani S.K., Arizti P., Reimer C.L., Sugrue M.M., Aaronson S.A., Lee S.W.
Cancer Res. 58:4439-4444(1998) [PubMed: 9766676] [Abstract]
Cited for: FUNCTION.
[9]"Large-scale characterization of HeLa cell nuclear phosphoproteins."
Beausoleil S.A., Jedrychowski M., Schwartz D., Elias J.E., Villen J., Li J., Cohn M.A., Cantley L.C., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004) [PubMed: 15302935] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-319; SER-330 AND SER-336, MASS SPECTROMETRY.
Tissue: Epithelium.
[10]"N-myc downstream-regulated gene 1 is mutated in hereditary motor and sensory neuropathy-Lom."
Kalaydjieva L., Gresham D., Gooding R., Heather L., Baas F., de Jonge R., Blechschmidt K., Angelicheva D., Chandler D., Worsley P., Rosenthal A., King R.H.M., Thomas P.K.
Am. J. Hum. Genet. 67:47-58(2000) [PubMed: 10831399] [Abstract]
Cited for: INVOLVEMENT IN CMT4D.
[11]"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
Cell 127:635-648(2006) [PubMed: 17081983] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-326; THR-328 AND SER-330, MASS SPECTROMETRY.
Tissue: Epithelium.
[12]"A probability-based approach for high-throughput protein phosphorylation analysis and site localization."
Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P.
Nat. Biotechnol. 24:1285-1292(2006) [PubMed: 16964243] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-330, MASS SPECTROMETRY.
Tissue: Epithelium.
[13]"Toward a global characterization of the phosphoproteome in prostate cancer cells: identification of phosphoproteins in the LNCaP cell line."
Giorgianni F., Zhao Y., Desiderio D.M., Beranova-Giorgianni S.
Electrophoresis 28:2027-2034(2007) [PubMed: 17487921] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-366, MASS SPECTROMETRY.
[14]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-326; THR-328; SER-330; SER-332; SER-333; THR-335; SER-336; SER-364 AND THR-375, MASS SPECTROMETRY.
+Additional computationally mapped references.

Hide | Top

Web resources

GeneReviews

Hide | Top

Cross-references

Sequence databases

D87953 mRNA. Translation: BAA13505.1.
X92845 mRNA. Translation: CAA63430.1.
AF004162 mRNA. Translation: AAC13419.1.
AF186190 Genomic DNA. No translation available.
AF192304 Genomic DNA. No translation available.
BC003175 mRNA. Translation: AAH03175.1.
AF230380 mRNA. Translation: AAF71305.1.
RefSeqNP_001128714.1.
NP_006087.2.
UniGeneHs.372914
Hs.618002

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

IntActQ92597.

Protein family/group databases

MEROPSS33.988.

PTM databases

PhosphoSiteQ92597.

Genome annotation databases

EnsemblENSG00000104419. Homo sapiens. [Contig view]
GeneID10397.
KEGGhsa:10397.

Organism-specific databases

H-InvDBHIX0019763.
HGNCHGNC:7679. NDRG1.
HPAHPA006881.
MIM601455. phenotype.
605262. gene.
Orphanet64749. Charcot-Marie-Tooth disease, type 4.
99950. Charcot-Marie-Tooth disease, type 4D.
PharmGKBPA31482.
GenAtlasSearch...
GeneCardsSearch...

Phylogenomic databases

HOGENOMQ92597.
HOVERGENQ92597.

Gene expression databases

ArrayExpressQ92597.
CleanExHS_DRG1.
HS_NDRG1.
GermOnlineENSG00000104419. Homo sapiens.

Family and domain databases

InterProIPR004142. Ndr.
[Graphical view]
PANTHERPTHR11034. Ndr. 1 hit.
PfamPF03096. Ndr. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio39394.
SOURCESearch...

Hide | Top

Entry information

Entry nameNDRG1_HUMAN
AccessionPrimary (citable) accession number: Q92597
Secondary accession number(s): O15207, Q9NYR6, Q9UK29
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: February 1, 1997
Last modified: November 25, 2008
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Hide | Top

Relevant documents

Human chromosome 8

Human chromosome 8: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Web resources · Cross-references · Entry information · Relevant documents