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Protein

UBX domain-containing protein 4

Gene

UBXN4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in endoplasmic reticulum-associated protein degradation (ERAD). Acts as a platform to recruit both UBQLN1 and VCP to the ER during ERAD (PubMed:19822669).2 Publications

GO - Biological processi

  • ER-associated ubiquitin-dependent protein catabolic process Source: UniProtKB
  • response to unfolded protein Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Unfolded protein response

Names & Taxonomyi

Protein namesi
Recommended name:
UBX domain-containing protein 4
Alternative name(s):
Erasin
UBX domain-containing protein 2
Gene namesi
Name:UBXN4
Synonyms:KIAA0242, UBXD2, UBXDC1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

HGNCiHGNC:14860. UBXN4.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 413413CytoplasmicSequence analysisAdd
BLAST
Intramembranei414 – 43421Sequence analysisAdd
BLAST
Topological domaini435 – 50874CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA37912.

Polymorphism and mutation databases

BioMutaiUBXN4.
DMDMi30913402.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 508508UBX domain-containing protein 4PRO_0000211027Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei489 – 4891PhosphothreonineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ92575.
MaxQBiQ92575.
PaxDbiQ92575.
PeptideAtlasiQ92575.
PRIDEiQ92575.

PTM databases

iPTMnetiQ92575.
PhosphoSiteiQ92575.

Expressioni

Tissue specificityi

Expressed in many tissues, including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Accumulates in Alzheimer disease-afflicted brains (at protein level).1 Publication

Inductioni

By ER stress-inducing agents such as tunicamycin, thapsigargin, DTT and the calcium ionophore A23187 (at protein level).1 Publication

Gene expression databases

BgeeiQ92575.
CleanExiHS_UBXN4.
ExpressionAtlasiQ92575. baseline and differential.
GenevisibleiQ92575. HS.

Organism-specific databases

HPAiHPA036325.

Interactioni

Subunit structurei

Directly interacts with VCP. Interacts with UBQLN1. Forms a complex with VCP and UBQLN1.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
UBQLN1Q9UMX03EBI-723441,EBI-741480
UBQLN1Q9UMX0-23EBI-723441,EBI-10173939
VCPP550722EBI-723441,EBI-355164

Protein-protein interaction databases

BioGridi116799. 26 interactions.
IntActiQ92575. 12 interactions.
MINTiMINT-1411585.
STRINGi9606.ENSP00000272638.

Structurei

Secondary structure

1
508
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi318 – 3258Combined sources
Beta strandi331 – 34111Combined sources
Helixi343 – 35311Combined sources
Beta strandi354 – 3574Combined sources
Beta strandi362 – 3676Combined sources
Helixi373 – 3775Combined sources
Beta strandi378 – 3803Combined sources
Helixi381 – 3833Combined sources
Beta strandi389 – 3935Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2KXJNMR-A317-397[»]
ProteinModelPortaliQ92575.
SMRiQ92575. Positions 317-397.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini315 – 39379UBXPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 200200Interaction with UBQLN11 PublicationAdd
BLAST

Domaini

The UBX domain is required for interaction with VCP.1 Publication
The intramembrane domain also contains the signal for ER targeting.1 Publication

Sequence similaritiesi

Contains 1 UBX domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2507. Eukaryota.
ENOG410ZR21. LUCA.
GeneTreeiENSGT00530000063500.
HOGENOMiHOG000007283.
HOVERGENiHBG056020.
InParanoidiQ92575.
OMAiAHNTSQA.
PhylomeDBiQ92575.
TreeFamiTF317466.

Family and domain databases

Gene3Di3.40.30.10. 1 hit.
InterProiIPR012336. Thioredoxin-like_fold.
IPR029071. Ubiquitin-rel_dom.
IPR001012. UBX_dom.
[Graphical view]
PfamiPF00789. UBX. 1 hit.
[Graphical view]
SMARTiSM00166. UBX. 1 hit.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEiPS50033. UBX. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q92575-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLWFQGAIPA AIATAKRSGA VFVVFVAGDD EQSTQMAASW EDDKVTEASS
60 70 80 90 100
NSFVAIKIDT KSEACLQFSQ IYPVVCVPSS FFIGDSGIPL EVIAGSVSAD
110 120 130 140 150
ELVTRIHKVR QMHLLKSETS VANGSQSESS VSTPSASFEP NNTCENSQSR
160 170 180 190 200
NAELCEIPPT SDTKSDTATG GESAGHATSS QEPSGCSDQR PAEDLNIRVE
210 220 230 240 250
RLTKKLEERR EEKRKEEEQR EIKKEIERRK TGKEMLDYKR KQEEELTKRM
260 270 280 290 300
LEERNREKAE DRAARERIKQ QIALDRAERA ARFAKTKEEV EAAKAAALLA
310 320 330 340 350
KQAEMEVKRE SYARERSTVA RIQFRLPDGS SFTNQFPSDA PLEEARQFAA
360 370 380 390 400
QTVGNTYGNF SLATMFPRRE FTKEDYKKKL LDLELAPSAS VVLLPAGRPT
410 420 430 440 450
ASIVHSSSGD IWTLLGTVLY PFLAIWRLIS NFLFSNPPPT QTSVRVTSSE
460 470 480 490 500
PPNPASSSKS EKREPVRKRV LEKRGDDFKK EGKIYRLRTQ DDGEDENNTW

NGNSTQQM
Length:508
Mass (Da):56,778
Last modified:May 16, 2003 - v2
Checksum:i2C3ABCF4D36124CC
GO

Sequence cautioni

The sequence BAA13437.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti458 – 4581S → R.
Corresponds to variant rs2304602 [ dbSNP | Ensembl ].
VAR_052686

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK292829 mRNA. Translation: BAF85518.1.
EF199840 mRNA. Translation: ABM90426.1.
D87684 mRNA. Translation: BAA13437.1. Different initiation.
BX647662 mRNA. No translation available.
AC011893 Genomic DNA. Translation: AAX88923.1.
CH471058 Genomic DNA. Translation: EAX11623.1.
BC035594 mRNA. Translation: AAH35594.1.
CCDSiCCDS42761.1.
RefSeqiNP_055422.1. NM_014607.3.
UniGeneiHs.591242.

Genome annotation databases

EnsembliENST00000272638; ENSP00000272638; ENSG00000144224.
GeneIDi23190.
KEGGihsa:23190.
UCSCiuc002tur.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK292829 mRNA. Translation: BAF85518.1.
EF199840 mRNA. Translation: ABM90426.1.
D87684 mRNA. Translation: BAA13437.1. Different initiation.
BX647662 mRNA. No translation available.
AC011893 Genomic DNA. Translation: AAX88923.1.
CH471058 Genomic DNA. Translation: EAX11623.1.
BC035594 mRNA. Translation: AAH35594.1.
CCDSiCCDS42761.1.
RefSeqiNP_055422.1. NM_014607.3.
UniGeneiHs.591242.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2KXJNMR-A317-397[»]
ProteinModelPortaliQ92575.
SMRiQ92575. Positions 317-397.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116799. 26 interactions.
IntActiQ92575. 12 interactions.
MINTiMINT-1411585.
STRINGi9606.ENSP00000272638.

PTM databases

iPTMnetiQ92575.
PhosphoSiteiQ92575.

Polymorphism and mutation databases

BioMutaiUBXN4.
DMDMi30913402.

Proteomic databases

EPDiQ92575.
MaxQBiQ92575.
PaxDbiQ92575.
PeptideAtlasiQ92575.
PRIDEiQ92575.

Protocols and materials databases

DNASUi23190.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000272638; ENSP00000272638; ENSG00000144224.
GeneIDi23190.
KEGGihsa:23190.
UCSCiuc002tur.4. human.

Organism-specific databases

CTDi23190.
GeneCardsiUBXN4.
HGNCiHGNC:14860. UBXN4.
HPAiHPA036325.
MIMi611216. gene.
neXtProtiNX_Q92575.
PharmGKBiPA37912.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2507. Eukaryota.
ENOG410ZR21. LUCA.
GeneTreeiENSGT00530000063500.
HOGENOMiHOG000007283.
HOVERGENiHBG056020.
InParanoidiQ92575.
OMAiAHNTSQA.
PhylomeDBiQ92575.
TreeFamiTF317466.

Miscellaneous databases

ChiTaRSiUBXN4. human.
GeneWikiiUBXD2.
GenomeRNAii23190.
PROiQ92575.
SOURCEiSearch...

Gene expression databases

BgeeiQ92575.
CleanExiHS_UBXN4.
ExpressionAtlasiQ92575. baseline and differential.
GenevisibleiQ92575. HS.

Family and domain databases

Gene3Di3.40.30.10. 1 hit.
InterProiIPR012336. Thioredoxin-like_fold.
IPR029071. Ubiquitin-rel_dom.
IPR001012. UBX_dom.
[Graphical view]
PfamiPF00789. UBX. 1 hit.
[Graphical view]
SMARTiSM00166. UBX. 1 hit.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEiPS50033. UBX. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Trachea.
  2. "Characterization of erasin (UBXD2): a new ER protein that promotes ER-associated protein degradation."
    Liang J., Yin C., Doong H., Fang S., Peterhoff C., Nixon R.A., Monteiro M.J.
    J. Cell Sci. 119:4011-4024(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH VCP, TISSUE SPECIFICITY, INDUCTION, DOMAIN UBX AND INTRAMEMBRANE DOMAIN.
  3. "Prediction of the coding sequences of unidentified human genes. VI. The coding sequences of 80 new genes (KIAA0201-KIAA0280) deduced by analysis of cDNA clones from cell line KG-1 and brain."
    Nagase T., Seki N., Ishikawa K., Ohira M., Kawarabayasi Y., Ohara O., Tanaka A., Kotani H., Miyajima N., Nomura N.
    DNA Res. 3:321-329(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Bone marrow.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Fetal skin.
  5. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
    Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H.
    , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
    Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Eye.
  8. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-489, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic kidney.
  9. Cited for: FUNCTION, INTERACTION WITH UBQLN1 AND VCP.
  10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  12. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  13. "Solution strcture of UBX domain of human UBXD2 protein."
    Wu Q., Huang H., Zhang J., Hu Q., Wu J., Shi Y.
    Submitted (MAY-2011) to the PDB data bank
    Cited for: STRUCTURE BY NMR OF 317-397.

Entry informationi

Entry nameiUBXN4_HUMAN
AccessioniPrimary (citable) accession number: Q92575
Secondary accession number(s): A8K9W4, Q4ZG56, Q8IYM5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 16, 2003
Last sequence update: May 16, 2003
Last modified: June 8, 2016
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.