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Protein

AP-3 complex subunit sigma-1

Gene

AP3S1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Part of the AP-3 complex, an adaptor-related complex which is not clathrin-associated. The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes. In concert with the BLOC-1 complex, AP-3 is required to target cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals.

GO - Molecular functioni

  1. protein transporter activity Source: InterPro
  2. transporter activity Source: ProtInc

GO - Biological processi

  1. anterograde axon cargo transport Source: UniProtKB
  2. anterograde synaptic vesicle transport Source: UniProtKB
  3. insulin receptor signaling pathway Source: ProtInc
  4. intracellular protein transport Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

ReactomeiREACT_19400. Golgi Associated Vesicle Biogenesis.

Names & Taxonomyi

Protein namesi
Recommended name:
AP-3 complex subunit sigma-1
Alternative name(s):
AP-3 complex subunit sigma-3A
Adaptor-related protein complex 3 subunit sigma-1
Clathrin-associated/assembly/adaptor protein, small 3
Sigma-3A-adaptin
Short name:
Sigma3A-adaptin
Sigma-adaptin 3a
Gene namesi
Name:AP3S1
Synonyms:CLAPS3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 5

Organism-specific databases

HGNCiHGNC:2013. AP3S1.

Subcellular locationi

Golgi apparatus. Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side
Note: Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex.

GO - Cellular componenti

  1. AP-3 adaptor complex Source: FlyBase
  2. AP-type membrane coat adaptor complex Source: ProtInc
  3. cytoplasmic vesicle membrane Source: UniProtKB-SubCell
  4. Golgi apparatus Source: UniProtKB-SubCell
  5. transport vesicle Source: ProtInc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA24862.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 193193AP-3 complex subunit sigma-1PRO_0000193815Add
BLAST

Proteomic databases

MaxQBiQ92572.
PaxDbiQ92572.
PRIDEiQ92572.

PTM databases

PhosphoSiteiQ92572.

Expressioni

Tissue specificityi

Present in all adult tissues examined.3 Publications

Gene expression databases

BgeeiQ92572.
CleanExiHS_AP3S1.
ExpressionAtlasiQ92572. baseline and differential.
GenevestigatoriQ92572.

Organism-specific databases

HPAiHPA049270.

Interactioni

Subunit structurei

Adaptor protein complex 3 (AP-3) is a heterotetramer composed of two large adaptins (delta-type subunit AP3D1 and beta-type subunit AP3B1 or AP3B2), a medium adaptin (mu-type subunit AP3M1 or AP3M2) and a small adaptin (sigma-type subunit APS1 or AP3S2). Interacts with AGAP1. AP-3 associates with the BLOC-1 complex (By similarity).By similarity

Protein-protein interaction databases

BioGridi107590. 22 interactions.
IntActiQ92572. 1 interaction.
STRINGi9606.ENSP00000325369.

Structurei

3D structure databases

ProteinModelPortaliQ92572.
SMRiQ92572. Positions 1-148.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG5030.
GeneTreeiENSGT00550000074761.
HOVERGENiHBG050517.
InParanoidiQ92572.
KOiK12399.
OMAiLSEMIIG.
OrthoDBiEOG7W41CZ.
PhylomeDBiQ92572.
TreeFamiTF300189.

Family and domain databases

InterProiIPR016635. AP_complex_ssu.
IPR022775. AP_mu_sigma_su.
IPR000804. Clathrin_sm-chain_CS.
IPR011012. Longin-like_dom.
[Graphical view]
PANTHERiPTHR11753. PTHR11753. 1 hit.
PfamiPF01217. Clat_adaptor_s. 1 hit.
[Graphical view]
PIRSFiPIRSF015588. AP_complex_sigma. 1 hit.
SUPFAMiSSF64356. SSF64356. 1 hit.
PROSITEiPS00989. CLAT_ADAPTOR_S. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q92572-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIKAILIFNN HGKPRLSKFY QPYSEDTQQQ IIRETFHLVS KRDENVCNFL
60 70 80 90 100
EGGLLIGGSD NKLIYRHYAT LYFVFCVDSS ESELGILDLI QVFVETLDKC
110 120 130 140 150
FENVCELDLI FHVDKVHNIL AEMVMGGMVL ETNMNEIVTQ IDAQNKLEKS
160 170 180 190
EAGLAGAPAR AVSAVKNMNL PEIPRNINIG DISIKVPNLP SFK
Length:193
Mass (Da):21,732
Last modified:February 1, 1997 - v1
Checksum:i1EF17F375881288D
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti158 – 1581P → L.
Corresponds to variant rs7733604 [ dbSNP | Ensembl ].
VAR_059111

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D63643 mRNA. Translation: BAA09798.1.
U91932 mRNA. Translation: AAD03779.1.
X99458 mRNA. Translation: CAA67823.1.
BT009833 mRNA. Translation: AAP88835.1.
CR450341 mRNA. Translation: CAG29337.1.
BC000804 mRNA. Translation: AAH00804.1.
CCDSiCCDS4123.1.
RefSeqiNP_001275.1. NM_001284.2.
UniGeneiHs.406191.

Genome annotation databases

EnsembliENST00000316788; ENSP00000325369; ENSG00000177879.
GeneIDi1176.
KEGGihsa:1176.
UCSCiuc003krk.3. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D63643 mRNA. Translation: BAA09798.1.
U91932 mRNA. Translation: AAD03779.1.
X99458 mRNA. Translation: CAA67823.1.
BT009833 mRNA. Translation: AAP88835.1.
CR450341 mRNA. Translation: CAG29337.1.
BC000804 mRNA. Translation: AAH00804.1.
CCDSiCCDS4123.1.
RefSeqiNP_001275.1. NM_001284.2.
UniGeneiHs.406191.

3D structure databases

ProteinModelPortaliQ92572.
SMRiQ92572. Positions 1-148.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi107590. 22 interactions.
IntActiQ92572. 1 interaction.
STRINGi9606.ENSP00000325369.

PTM databases

PhosphoSiteiQ92572.

Proteomic databases

MaxQBiQ92572.
PaxDbiQ92572.
PRIDEiQ92572.

Protocols and materials databases

DNASUi1176.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000316788; ENSP00000325369; ENSG00000177879.
GeneIDi1176.
KEGGihsa:1176.
UCSCiuc003krk.3. human.

Organism-specific databases

CTDi1176.
GeneCardsiGC05P115177.
HGNCiHGNC:2013. AP3S1.
HPAiHPA049270.
MIMi601507. gene.
neXtProtiNX_Q92572.
PharmGKBiPA24862.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG5030.
GeneTreeiENSGT00550000074761.
HOVERGENiHBG050517.
InParanoidiQ92572.
KOiK12399.
OMAiLSEMIIG.
OrthoDBiEOG7W41CZ.
PhylomeDBiQ92572.
TreeFamiTF300189.

Enzyme and pathway databases

ReactomeiREACT_19400. Golgi Associated Vesicle Biogenesis.

Miscellaneous databases

ChiTaRSiAP3S1. human.
GeneWikiiAP3S1.
GenomeRNAii1176.
NextBioi4862.
PROiQ92572.
SOURCEiSearch...

Gene expression databases

BgeeiQ92572.
CleanExiHS_AP3S1.
ExpressionAtlasiQ92572. baseline and differential.
GenevestigatoriQ92572.

Family and domain databases

InterProiIPR016635. AP_complex_ssu.
IPR022775. AP_mu_sigma_su.
IPR000804. Clathrin_sm-chain_CS.
IPR011012. Longin-like_dom.
[Graphical view]
PANTHERiPTHR11753. PTHR11753. 1 hit.
PfamiPF01217. Clat_adaptor_s. 1 hit.
[Graphical view]
PIRSFiPIRSF015588. AP_complex_sigma. 1 hit.
SUPFAMiSSF64356. SSF64356. 1 hit.
PROSITEiPS00989. CLAT_ADAPTOR_S. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning, expression pattern and mapping to 12p 13.2 --> p13.1 of CLAPS3, a gene encoding a novel clathrin-adaptor small chain."
    Watanabe T.K., Shimizu F., Nagata M., Takaichi A., Fujiwara T., Nakamura Y., Takahashi E., Hirai Y.
    Cytogenet. Cell Genet. 73:214-217(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Tissue: Fetal brain.
  2. "Characterization of the adaptor-related protein complex, AP-3."
    Simpson F., Peden A.A., Christopoulou L., Robinson M.S.
    J. Cell Biol. 137:835-845(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Tissue: Placenta.
  3. "AP-3: an adaptor-like protein complex with ubiquitous expression."
    Dell'Angelica E.C., Ohno H., Ooi C.E., Rabinovich E., Roche K.W., Bonifacino J.S.
    EMBO J. 16:917-928(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Tissue: Brain, Pancreas and Placenta.
  4. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
    Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
    Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
    Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Placenta.
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiAP3S1_HUMAN
AccessioniPrimary (citable) accession number: Q92572
Secondary accession number(s): O00647
, O00676, O00721, O00727, Q53XL4, Q6ICQ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2003
Last sequence update: February 1, 1997
Last modified: March 4, 2015
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.