Q92572 (AP3S1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
AP-3 complex subunit sigma-1

Alternative name(s):
AP-3 complex subunit sigma-3A
Adapter-related protein complex 3 sigma-1 subunit
Clathrin-associated/assembly/adapter protein, small 3
Sigma-3A-adaptin
Short name=Sigma3A-adaptin
Sigma-adaptin 3a

Gene names

Name:	AP3S1
Synonyms:	CLAPS3

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	193 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Part of the AP-3 complex, an adapter-related complex which is not clathrin-associated. The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes.
Subunit structure	Adaptor protein complex 3 (AP-3) is an heterotetramer composed of two large adaptins (delta-type subunit AP3D1 and beta-type subunit AP3B1 or AP3B2), a medium adaptin (mu-type subunit AP3M1 or AP3M2) and a small adaptin (sigma-type subunit APS1 or AP3S2). Interacts with AGAP1 By similarity.
Subcellular location	Golgi apparatus. Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Note: Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex.
Tissue specificity	Present in all adult tissues examined. Ref.1 Ref.2 Ref.3
Sequence similarities	Belongs to the adaptor complexes small subunit family.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Cytoplasmic vesicle Golgi apparatus Membrane
Coding sequence diversity	Polymorphism
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	insulin receptor signaling pathway Traceable author statement. Source: ProtInc intracellular protein transport Inferred from electronic annotation. Source: InterPro vesicle-mediated transport Inferred from electronic annotation. Source: InterPro
Cellular component	AP-type membrane coat adaptor complex Traceable author statement. Source: ProtInc Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-SubCell cytoplasmic vesicle membrane Inferred from electronic annotation. Source: UniProtKB-SubCell transport vesicle Traceable author statement. Source: ProtInc
Molecular function	protein binding Inferred from physical interaction. Source: UniProtKB protein transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 193	193	AP-3 complex subunit sigma-1		PRO_0000193815
Natural variations
	Natural variant	158	1	P → L. Corresponds to variant rs7733604 [ dbSNP | Ensembl ].		VAR_059111

Sequences

	Sequence		Length	Mass (Da)
	Q92572 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 1EF17F375881288D	FASTA	193	21,732
	10 20 30 40 50 60 MIKAILIFNN HGKPRLSKFY QPYSEDTQQQ IIRETFHLVS KRDENVCNFL EGGLLIGGSD 70 80 90 100 110 120 NKLIYRHYAT LYFVFCVDSS ESELGILDLI QVFVETLDKC FENVCELDLI FHVDKVHNIL 130 140 150 160 170 180 AEMVMGGMVL ETNMNEIVTQ IDAQNKLEKS EAGLAGAPAR AVSAVKNMNL PEIPRNINIG 190 DISIKVPNLP SFK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, expression pattern and mapping to 12p 13.2 --> p13.1 of CLAPS3, a gene encoding a novel clathrin-adaptor small chain." Watanabe T.K., Shimizu F., Nagata M., Takaichi A., Fujiwara T., Nakamura Y., Takahashi E., Hirai Y. Cytogenet. Cell Genet. 73:214-217(1996) [PubMed: 8697810] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Tissue: Fetal brain.
[2]	"Characterization of the adaptor-related protein complex, AP-3." Simpson F., Peden A.A., Christopoulou L., Robinson M.S. J. Cell Biol. 137:835-845(1997) [PubMed: 9151686] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Tissue: Placenta.
[3]	"AP-3: an adaptor-like protein complex with ubiquitous expression." Dell'Angelica E.C., Ohno H., Ooi C.E., Rabinovich E., Roche K.W., Bonifacino J.S. EMBO J. 16:917-928(1997) [PubMed: 9118953] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Tissue: Brain, Pancreas and Placenta.
[4]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Placenta.
[7]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D63643 mRNA. Translation: BAA09798.1. U91932 mRNA. Translation: AAD03779.1. X99458 mRNA. Translation: CAA67823.1. BT009833 mRNA. Translation: AAP88835.1. CR450341 mRNA. Translation: CAG29337.1. BC000804 mRNA. Translation: AAH00804.1.
IPI	IPI00014624.
RefSeq	NP_001275.1. NM_001284.2.
UniGene	Hs.728956.
3D structure databases
ProteinModelPortal	Q92572.
SMR	Q92572. Positions 1-148.
ModBase	Search...
Protein-protein interaction databases
STRING	Q92572.
PTM databases
PhosphoSite	Q92572.
Polymorphism databases
DMDM	33112222.
Proteomic databases
PRIDE	Q92572.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000316788; ENSP00000325369; ENSG00000177879.
GeneID	1176.
KEGG	hsa:1176.
UCSC	uc003krl.1. human.
Organism-specific databases
CTD	1176.
GeneCards	GC05P115177.
H-InvDB	HIX0018988.
HGNC	HGNC:2013. AP3S1.
MIM	601507. gene.
neXtProt	NX_Q92572.
PharmGKB	PA24862.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG17381.
GeneTree	ENSGT00550000074761.
HOVERGEN	HBG050517.
InParanoid	Q92572.
OMA	KSMNIPQ.
OrthoDB	EOG4GF3G5.
PhylomeDB	Q92572.
Enzyme and pathway databases
Reactome	REACT_11123. Membrane Trafficking.
Gene expression databases
Bgee	Q92572.
CleanEx	HS_AP3S1.
Genevestigator	Q92572.
GermOnline	ENSG00000177879. Homo sapiens.
Family and domain databases
InterPro	IPR016635. AP_complex_ssu. IPR022775. AP_mu_sigma_su. IPR000804. Clathrin_sm-chain_CS. IPR011012. Longin-like. [Graphical view]
KO	K12399.
PANTHER	PTHR11753. PTHR11753. 1 hit.
Pfam	PF01217. Clat_adaptor_s. 1 hit. [Graphical view]
PIRSF	PIRSF015588. AP_complex_sigma. 1 hit.
SUPFAM	SSF64356. Longin_like. 1 hit.
PROSITE	PS00989. CLAT_ADAPTOR_S. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	4862.
SOURCE	Search...

Entry information

Entry name

AP3S1_HUMAN

Accession

Primary (citable) accession number: Q92572
Secondary accession number(s): O00647 , O00676, O00721, O00727, Q53XL4, Q6ICQ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2003
Last sequence update:	February 1, 1997
Last modified:	January 25, 2012
This is version 96 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.