Reviewed, UniProtKB/Swiss-Prot Q92569 (P55G_HUMAN)

Names and origin

Protein names

Recommended name:
    Phosphatidylinositol 3-kinase regulatory subunit gamma
      Short name=PtdIns-3-kinase regulatory subunit gamma
      Short name=PI3-kinase regulatory subunit gamma
      Short name=PI3K regulatory subunit gamma
Alternative name(s):
    Phosphatidylinositol 3-kinase 55 kDa regulatory subunit gamma
      Short name=PtdIns-3-kinase regulatory subunit p55-gamma
      Short name=PI3-kinase subunit p55-gamma
    p55PIK

Gene names

Name:

PIK3R3

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	461 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain and regulates their kinase activity. During insulin stimulation, it also binds to IRS-1.
Subunit structure	Heterodimer of a p110 (catalytic) and a p55 (regulatory) subunits.
Tissue specificity	Highest levels in brain and testis. Lower levels in adipose tissue, kidney, heart, lung and skeletal muscle.
Sequence similarities	Belongs to the PI3K p85 subunit family. Contains 2 SH2 domains.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Repeat SH2 domain
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	insulin receptor signaling pathway Ref.2 Traceable author statement. Source: ProtInc
Cellular component	phosphoinositide 3-kinase complex Inferred from electronic annotation. Source: InterPro
Molecular function	1-phosphatidylinositol-3-kinase activity Ref.2 Traceable author statement. Source: ProtInc phosphoinositide 3-kinase regulator activity Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CD19	P15391	1	EBI-79893,EBI-79902

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q92569-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q92569-2)

The sequence of this isoform differs from the canonical sequence as follows:
     256-314: Missing.

Isoform 3 (identifier: Q92569-3)

The sequence of this isoform differs from the canonical sequence as follows:
     36-71: Missing.
     256-314: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 461	461	Phosphatidylinositol 3-kinase regulatory subunit gamma		PRO_0000080767
Regions
	Domain	65 – 160	96	SH2 1
	Domain	358 – 452	95	SH2 2
	Compositional bias	34 – 44	11	Pro-rich
Amino acid modifications
	Modified residue	199	1	Phosphotyrosine Ref.6
	Modified residue	341	1	Phosphotyrosine By similarity
Natural variations
	Alternative sequence	36 – 71	36	Missing in isoform 3.		VSP_004713
	Alternative sequence	256 – 314	59	Missing in isoform 2 and isoform 3.		VSP_004714
	Natural variant	283	1	K → N: dbSNP rs785467. Ref.4		VAR_047153
Experimental info
	Sequence conflict	21	1	P → L in AAC39696. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: C03ECFF22BEB50B9	FASTA	461	54,462
	10 20 30 40 50 60 MYNTVWSMDR DDADWREVMM PYSTELIFYI EMDPPALPPK PPKPMTSAVP NGMKDSSVSL 70 80 90 100 110 120 QDAEWYWGDI SREEVNDKLR DMPDGTFLVR DASTKMQGDY TLTLRKGGNN KLIKIYHRDG 130 140 150 160 170 180 KYGFSDPLTF NSVVELINHY HHESLAQYNP KLDVKLMYPV SRYQQDQLVK EDNIDAVGKK 190 200 210 220 230 240 LQEYHSQYQE KSKEYDRLYE EYTRTSQEIQ MKRTAIEAFN ETIKIFEEQC HTQEQHSKEY 250 260 270 280 290 300 IERFRREGNE KEIERIMMNY DKLKSRLGEI HDSKMRLEQD LKKQALDNRE IDKKMNSIKP 310 320 330 340 350 360 DLIQLRKIRD QHLVWLNHKG VRQKRLNVWL GIKNEDADEN YFINEEDENL PHYDEKTWFV 370 380 390 400 410 420 EDINRVQAED LLYGKPDGAF LIRESSKKGC YACSVVADGE VKHCVIYSTA RGYGFAEPYN 430 440 450 460 LYSSLKELVL HYQQTSLVQH NDSLNVRLAY PVHAQMPSLC R
	Isoform 2. Checksum: 054C2111B68F17A6	FASTA	402	47,348
	10 20 30 40 50 60 MYNTVWSMDR DDADWREVMM PYSTELIFYI EMDPPALPPK PPKPMTSAVP NGMKDSSVSL 70 80 90 100 110 120 QDAEWYWGDI SREEVNDKLR DMPDGTFLVR DASTKMQGDY TLTLRKGGNN KLIKIYHRDG 130 140 150 160 170 180 KYGFSDPLTF NSVVELINHY HHESLAQYNP KLDVKLMYPV SRYQQDQLVK EDNIDAVGKK 190 200 210 220 230 240 LQEYHSQYQE KSKEYDRLYE EYTRTSQEIQ MKRTAIEAFN ETIKIFEEQC HTQEQHSKEY 250 260 270 280 290 300 IERFRREGNE KEIERWLNHK GVRQKRLNVW LGIKNEDADE NYFINEEDEN LPHYDEKTWF 310 320 330 340 350 360 VEDINRVQAE DLLYGKPDGA FLIRESSKKG CYACSVVADG EVKHCVIYST ARGYGFAEPY 370 380 390 400 NLYSSLKELV LHYQQTSLVQ HNDSLNVRLA YPVHAQMPSL CR
	Isoform 3. Checksum: F8B6B90E29AA2DF6	FASTA	366	43,465
	10 20 30 40 50 60 MYNTVWSMDR DDADWREVMM PYSTELIFYI EMDPPREEVN DKLRDMPDGT FLVRDASTKM 70 80 90 100 110 120 QGDYTLTLRK GGNNKLIKIY HRDGKYGFSD PLTFNSVVEL INHYHHESLA QYNPKLDVKL 130 140 150 160 170 180 MYPVSRYQQD QLVKEDNIDA VGKKLQEYHS QYQEKSKEYD RLYEEYTRTS QEIQMKRTAI 190 200 210 220 230 240 EAFNETIKIF EEQCHTQEQH SKEYIERFRR EGNEKEIERW LNHKGVRQKR LNVWLGIKNE 250 260 270 280 290 300 DADENYFINE EDENLPHYDE KTWFVEDINR VQAEDLLYGK PDGAFLIRES SKKGCYACSV 310 320 330 340 350 360 VADGEVKHCV IYSTARGYGF AEPYNLYSSL KELVLHYQQT SLVQHNDSLN VRLAYPVHAQ MPSLCR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of human p55pik." Suzuki T. Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"Cloning of human p55 gamma, a regulatory subunit of phosphatidylinositol 3-kinase, by a yeast two-hybrid library screen with the insulin-like growth factor-I receptor." Dey B.R., Furlanetto R.W., Nissley S.P. Gene 209:175-183(1998) [PubMed: 9524259] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3). Tissue: Fetal brain.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
[4]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed: 16710414] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT ASN-283.
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-199, MASS SPECTROMETRY. Tissue: T-cell.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D88532 mRNA. Translation: BAA13636.1. AF028785 mRNA. Translation: AAC39696.1. AK313726 mRNA. Translation: BAG36468.1. AL358075 Genomic DNA. Translation: CAI21702.1. AL358075 Genomic DNA. Translation: CAI21703.1. CH471059 Genomic DNA. Translation: EAX06944.1. CH471059 Genomic DNA. Translation: EAX06945.1.
IPI	IPI00220498. IPI00220499. IPI00513745.
RefSeq	NP_001107644.1. NP_003620.3.
UniGene	Hs.655387
3D structure databases
SMR	Q92569. Positions 57-171, 163-331, 348-455.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q92569. 7 interactions.
STRING	Q92569.
PTM databases
PhosphoSite	Q92569.
Proteomic databases
PRIDE	Q92569.
Genome annotation databases
Ensembl	ENST00000262741; ENSP00000262741; ENSG00000117461; Homo sapiens. [Genome view] ENST00000372006; ENSP00000361075; ENSG00000117461; Homo sapiens. [Genome view] ENST00000420542; ENSP00000412546; ENSG00000117461; Homo sapiens. [Genome view]
GeneID	8503.
KEGG	hsa:8503.
UCSC	uc009vyb.1. human.
Organism-specific databases
CTD	8503.
GeneCards	GC01M046217.
H-InvDB	HIX0000542.
HGNC	HGNC:8981. PIK3R3.
HPA	HPA005751.
MIM	606076. gene.
PharmGKB	PA33314.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG15178.
HOVERGEN	Q92569.
InParanoid	Q92569.
Enzyme and pathway databases
BioCyc	MetaCyc:ENSG00000117461-MONOMER.
Pathway_Interaction_DB	pi3kcipathway. Class I PI3K signaling events. trail_pathway. TRAIL signaling pathway.
Gene expression databases
ArrayExpress	Q92569.
Bgee	Q92569.
CleanEx	HS_PIK3R3.
Genevestigator	Q92569.
GermOnline	ENSG00000117461. Homo sapiens.
Family and domain databases
InterPro	IPR001720. PI3kinase_P85. IPR000980. SH2. [Graphical view]
Gene3D	G3DSA:3.30.505.10. SH2. 2 hits.
PANTHER	PTHR10155. PI3kinase_P85. 1 hit.
Pfam	PF00017. SH2. 2 hits. [Graphical view]
PRINTS	PR00678. PI3KINASEP85. PR00401. SH2DOMAIN.
SMART	SM00252. SH2. 2 hits. [Graphical view]
PROSITE	PS50001. SH2. 2 hits. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

P55G_HUMAN

Accession

Primary (citable) accession number: Q92569
Secondary accession number(s): B2R9C1 , O60482, Q5T4P1, Q5T4P2

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	February 1, 1997
Last modified:	February 9, 2010
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.