Q92569 (P55G_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphatidylinositol 3-kinase regulatory subunit gamma
Short name=PI3-kinase regulatory subunit gamma
Short name=PI3K regulatory subunit gamma
Short name=PtdIns-3-kinase regulatory subunit gamma

Alternative name(s):
Phosphatidylinositol 3-kinase 55 kDa regulatory subunit gamma
Short name=PI3-kinase subunit p55-gamma
Short name=PtdIns-3-kinase regulatory subunit p55-gamma
p55PIK

Gene names

Name:

PIK3R3

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	461 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain and regulates their kinase activity. During insulin stimulation, it also binds to IRS-1.
Subunit structure	Heterodimer of a regulatory subunit PIK3R3 and a p110 catalytic subunit (PIK3CA, PIK3CB or PIK3CD). Interacts with AXL. Ref.6
Tissue specificity	Highest levels in brain and testis. Lower levels in adipose tissue, kidney, heart, lung and skeletal muscle.
Sequence similarities	Belongs to the PI3K p85 subunit family. Contains 2 SH2 domains.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Repeat SH2 domain
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	insulin receptor signaling pathway Traceable author statement Ref.1. Source: ProtInc regulation of phosphatidylinositol 3-kinase activity Inferred from electronic annotation. Source: GOC small molecule metabolic process Traceable author statement. Source: Reactome
Cellular_component	cytosol Traceable author statement. Source: Reactome phosphatidylinositol 3-kinase complex Inferred from electronic annotation. Source: Compara
Molecular_function	1-phosphatidylinositol-3-kinase activity Traceable author statement Ref.1. Source: ProtInc 1-phosphatidylinositol-3-kinase regulator activity Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q92569-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q92569-2)

The sequence of this isoform differs from the canonical sequence as follows:
     256-314: Missing.

Isoform 3 (identifier: Q92569-3)

The sequence of this isoform differs from the canonical sequence as follows:
     36-71: Missing.
     256-314: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 461	461	Phosphatidylinositol 3-kinase regulatory subunit gamma		PRO_0000080767
Regions
	Domain	65 – 160	96	SH2 1
	Domain	358 – 452	95	SH2 2
	Compositional bias	34 – 44	11	Pro-rich
Amino acid modifications
	Modified residue	199	1	Phosphotyrosine By similarity
	Modified residue	341	1	Phosphotyrosine By similarity
Natural variations
	Alternative sequence	36 – 71	36	Missing in isoform 3.		VSP_004713
	Alternative sequence	256 – 314	59	Missing in isoform 2 and isoform 3.		VSP_004714
	Natural variant	283	1	N → K. Ref.1 Ref.2 Ref.3 Ref.5 Corresponds to variant rs785467 [ dbSNP | Ensembl ].		VAR_047153
Experimental info
	Sequence conflict	21	1	P → L in AAC39696. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified January 11, 2011. Version 2. Checksum: 303BCC2F9EEE5364	FASTA	461	54,448
	10 20 30 40 50 60 MYNTVWSMDR DDADWREVMM PYSTELIFYI EMDPPALPPK PPKPMTSAVP NGMKDSSVSL 70 80 90 100 110 120 QDAEWYWGDI SREEVNDKLR DMPDGTFLVR DASTKMQGDY TLTLRKGGNN KLIKIYHRDG 130 140 150 160 170 180 KYGFSDPLTF NSVVELINHY HHESLAQYNP KLDVKLMYPV SRYQQDQLVK EDNIDAVGKK 190 200 210 220 230 240 LQEYHSQYQE KSKEYDRLYE EYTRTSQEIQ MKRTAIEAFN ETIKIFEEQC HTQEQHSKEY 250 260 270 280 290 300 IERFRREGNE KEIERIMMNY DKLKSRLGEI HDSKMRLEQD LKNQALDNRE IDKKMNSIKP 310 320 330 340 350 360 DLIQLRKIRD QHLVWLNHKG VRQKRLNVWL GIKNEDADEN YFINEEDENL PHYDEKTWFV 370 380 390 400 410 420 EDINRVQAED LLYGKPDGAF LIRESSKKGC YACSVVADGE VKHCVIYSTA RGYGFAEPYN 430 440 450 460 LYSSLKELVL HYQQTSLVQH NDSLNVRLAY PVHAQMPSLC R
	Isoform 2 [UniParc]. Checksum: 054C2111B68F17A6	FASTA	402	47,348
	10 20 30 40 50 60 MYNTVWSMDR DDADWREVMM PYSTELIFYI EMDPPALPPK PPKPMTSAVP NGMKDSSVSL 70 80 90 100 110 120 QDAEWYWGDI SREEVNDKLR DMPDGTFLVR DASTKMQGDY TLTLRKGGNN KLIKIYHRDG 130 140 150 160 170 180 KYGFSDPLTF NSVVELINHY HHESLAQYNP KLDVKLMYPV SRYQQDQLVK EDNIDAVGKK 190 200 210 220 230 240 LQEYHSQYQE KSKEYDRLYE EYTRTSQEIQ MKRTAIEAFN ETIKIFEEQC HTQEQHSKEY 250 260 270 280 290 300 IERFRREGNE KEIERWLNHK GVRQKRLNVW LGIKNEDADE NYFINEEDEN LPHYDEKTWF 310 320 330 340 350 360 VEDINRVQAE DLLYGKPDGA FLIRESSKKG CYACSVVADG EVKHCVIYST ARGYGFAEPY 370 380 390 400 NLYSSLKELV LHYQQTSLVQ HNDSLNVRLA YPVHAQMPSL CR
	Isoform 3 [UniParc]. Checksum: F8B6B90E29AA2DF6	FASTA	366	43,465
	10 20 30 40 50 60 MYNTVWSMDR DDADWREVMM PYSTELIFYI EMDPPREEVN DKLRDMPDGT FLVRDASTKM 70 80 90 100 110 120 QGDYTLTLRK GGNNKLIKIY HRDGKYGFSD PLTFNSVVEL INHYHHESLA QYNPKLDVKL 130 140 150 160 170 180 MYPVSRYQQD QLVKEDNIDA VGKKLQEYHS QYQEKSKEYD RLYEEYTRTS QEIQMKRTAI 190 200 210 220 230 240 EAFNETIKIF EEQCHTQEQH SKEYIERFRR EGNEKEIERW LNHKGVRQKR LNVWLGIKNE 250 260 270 280 290 300 DADENYFINE EDENLPHYDE KTWFVEDINR VQAEDLLYGK PDGAFLIRES SKKGCYACSV 310 320 330 340 350 360 VADGEVKHCV IYSTARGYGF AEPYNLYSSL KELVLHYQQT SLVQHNDSLN VRLAYPVHAQ MPSLCR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of human p55 gamma, a regulatory subunit of phosphatidylinositol 3-kinase, by a yeast two-hybrid library screen with the insulin-like growth factor-I receptor." Dey B.R., Furlanetto R.W., Nissley S.P. Gene 209:175-183(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3), VARIANT LYS-283. Tissue: Fetal brain.
[2]	"Molecular cloning of human p55pik." Suzuki T. Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT LYS-283.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT LYS-283. Tissue: Brain.
[4]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT LYS-283.
[6]	"Interaction of Axl receptor tyrosine kinase with C1-TEN, a novel C1 domain-containing protein with homology to tensin." Hafizi S., Alindri F., Karlsson R., Dahlbaeck B. Biochem. Biophys. Res. Commun. 299:793-800(2002) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH AXL.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF028785 mRNA. Translation: AAC39696.1. D88532 mRNA. Translation: BAA13636.1. AK313726 mRNA. Translation: BAG36468.1. AL358075 Genomic DNA. Translation: CAI21702.1. AL358075 Genomic DNA. Translation: CAI21703.1. CH471059 Genomic DNA. Translation: EAX06946.1. CH471059 Genomic DNA. Translation: EAX06947.1. CH471059 Genomic DNA. Translation: EAX06944.1. CH471059 Genomic DNA. Translation: EAX06945.1.
IPI	IPI00220498. IPI00220499. IPI00513745.
RefSeq	NP_001107644.1. NM_001114172.1. NP_003620.3. NM_003629.3.
UniGene	Hs.655387.
3D structure databases
ProteinModelPortal	Q92569.
SMR	Q92569. Positions 58-455.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q92569. 17 interactions.
MINT	MINT-1490763.
STRING	9606.ENSP00000262741.
PTM databases
PhosphoSite	Q92569.
Polymorphism databases
DMDM	12643788.
Proteomic databases
PaxDb	Q92569.
PRIDE	Q92569.
Protocols and materials databases
DNASU	8503.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000262741; ENSP00000262741; ENSG00000117461. ENST00000354242; ENSP00000346188; ENSG00000117461. ENST00000372006; ENSP00000361075; ENSG00000117461. ENST00000420542; ENSP00000412546; ENSG00000117461. ENST00000423209; ENSP00000391431; ENSG00000117461.
GeneID	8503.
KEGG	hsa:8503.
UCSC	uc001cpb.4. human. uc009vyb.3. human.
Organism-specific databases
CTD	8503.
GeneCards	GC01M046505.
H-InvDB	HIX0000542.
HGNC	HGNC:8981. PIK3R3.
HPA	HPA005751.
MIM	606076. gene.
neXtProt	NX_Q92569.
PharmGKB	PA33314.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG263689.
HOVERGEN	HBG082100.
InParanoid	Q92569.
KO	K02649.
OrthoDB	EOG4QFWD5.
PhylomeDB	Q92569.
Enzyme and pathway databases
BioCyc	MetaCyc:ENSG00000117461-MONOMER.
Pathway_Interaction_DB	pi3kcipathway. Class I PI3K signaling events. trail_pathway. TRAIL signaling pathway.
Reactome	REACT_111102. Signal Transduction. REACT_111217. Metabolism. REACT_116125. Disease. REACT_604. Hemostasis. REACT_6900. Immune System.
SignaLink	Q92569.
Gene expression databases
ArrayExpress	Q92569.
Bgee	Q92569.
CleanEx	HS_PIK3R3.
Genevestigator	Q92569.
GermOnline	ENSG00000117461. Homo sapiens.
Family and domain databases
Gene3D	3.30.505.10. 2 hits.
InterPro	IPR001720. PI3kinase_P85. IPR000980. SH2. [Graphical view]
PANTHER	PTHR10155. PTHR10155. 1 hit.
Pfam	PF00017. SH2. 2 hits. [Graphical view]
PRINTS	PR00678. PI3KINASEP85. PR00401. SH2DOMAIN.
SMART	SM00252. SH2. 2 hits. [Graphical view]
PROSITE	PS50001. SH2. 2 hits. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	8503.
NextBio	31821.
SOURCE	Search...

Entry information

Entry name

P55G_HUMAN

Accession

Primary (citable) accession number: Q92569
Secondary accession number(s): B2R9C1 , D3DQ12, O60482, Q5T4P1, Q5T4P2

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	January 11, 2011
Last modified:	May 29, 2013
This is version 115 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.