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Protein

Rap guanine nucleotide exchange factor 5

Gene

RAPGEF5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Guanine nucleotide exchange factor (GEF) for RAP1A, RAP2A and MRAS/M-Ras-GTP. Its association with MRAS inhibits Rap1 activation.2 Publications

GO - Molecular functioni

  • GTP-dependent protein binding Source: UniProtKB
  • Rap guanyl-nucleotide exchange factor activity Source: UniProtKB
  • Ras guanyl-nucleotide exchange factor activity Source: UniProtKB

GO - Biological processi

  • nervous system development Source: UniProtKB
  • small GTPase mediated signal transduction Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Guanine-nucleotide releasing factor

Enzyme and pathway databases

BioCyciZFISH:ENSG00000136237-MONOMER.
SIGNORiQ92565.

Names & Taxonomyi

Protein namesi
Recommended name:
Rap guanine nucleotide exchange factor 5
Alternative name(s):
Guanine nucleotide exchange factor for Rap1
M-Ras-regulated Rap GEF
Short name:
MR-GEF
Related to Epac
Short name:
Repac
Gene namesi
Name:RAPGEF5
Synonyms:GFR, KIAA0277, MRGEF
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 7

Organism-specific databases

HGNCiHGNC:16862. RAPGEF5.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi9771.
OpenTargetsiENSG00000136237.
PharmGKBiPA134902361.

Polymorphism and mutation databases

DMDMi32171396.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000688731 – 580Rap guanine nucleotide exchange factor 5Add BLAST580

Proteomic databases

MaxQBiQ92565.
PaxDbiQ92565.
PeptideAtlasiQ92565.
PRIDEiQ92565.

PTM databases

iPTMnetiQ92565.
PhosphoSitePlusiQ92565.

Expressioni

Tissue specificityi

Widely expressed with highest levels in brain.

Gene expression databases

BgeeiENSG00000136237.
CleanExiHS_RAPGEF5.
ExpressionAtlasiQ92565. baseline and differential.
GenevisibleiQ92565. HS.

Organism-specific databases

HPAiHPA057821.

Interactioni

GO - Molecular functioni

  • GTP-dependent protein binding Source: UniProtKB

Protein-protein interaction databases

BioGridi115116. 28 interactors.
IntActiQ92565. 3 interactors.
STRINGi9606.ENSP00000343656.

Structurei

Secondary structure

1580
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi245 – 248Combined sources4
Beta strandi250 – 252Combined sources3
Beta strandi254 – 257Combined sources4
Helixi266 – 276Combined sources11
Helixi280 – 282Combined sources3
Beta strandi283 – 288Combined sources6
Beta strandi301 – 304Combined sources4
Beta strandi313 – 318Combined sources6
Beta strandi321 – 323Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WGYNMR-A241-331[»]
ProteinModelPortaliQ92565.
SMRiQ92565.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ92565.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini68 – 201N-terminal Ras-GEFPROSITE-ProRule annotationAdd BLAST134
Domaini345 – 579Ras-GEFPROSITE-ProRule annotationAdd BLAST235

Sequence similaritiesi

Contains 1 N-terminal Ras-GEF domain.PROSITE-ProRule annotation
Contains 1 Ras-GEF domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2378. Eukaryota.
ENOG410XPX9. LUCA.
GeneTreeiENSGT00860000133664.
HOGENOMiHOG000230545.
HOVERGENiHBG056985.
InParanoidiQ92565.
KOiK08019.
PhylomeDBiQ92565.

Family and domain databases

CDDicd00155. RasGEF. 1 hit.
cd06224. REM. 1 hit.
Gene3Di1.10.840.10. 1 hit.
InterProiIPR000651. Ras-like_Gua-exchang_fac_N.
IPR019804. Ras_G-nucl-exch_fac_CS.
IPR023578. Ras_GEF_dom.
IPR001895. RASGEF_cat_dom.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF00617. RasGEF. 1 hit.
PF00618. RasGEF_N. 1 hit.
[Graphical view]
SMARTiSM00147. RasGEF. 1 hit.
SM00229. RasGEFN. 1 hit.
[Graphical view]
SUPFAMiSSF48366. SSF48366. 2 hits.
SSF54236. SSF54236. 1 hit.
PROSITEiPS00720. RASGEF. 1 hit.
PS50009. RASGEF_CAT. 1 hit.
PS50212. RASGEF_NTER. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q92565-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGSSRLRVFD PHLERKDSAA ALSDRELPLP TFDVPYFKYI DEEDEDDEWS
60 70 80 90 100
SRSQSSTEDD SVDSLLSDRY VVVSGTPEKI LEHLLNDLHL EEVQDKETET
110 120 130 140 150
LLDDFLLTYT VFMTTDDLCQ ALLRHYSAKK YQGKEENSDV PRRKRKVLHL
160 170 180 190 200
VSQWIALYKD WLPEDEHSKM FLKTIYRNVL DDVYEYPILE KELKEFQKIL
210 220 230 240 250
GMHRRHTVDE YSPQKKNKAL FHQFSLKENW LQHRGTVTET EEIFCHVYIT
260 270 280 290 300
EHSYVSVKAK VSSIAQEILK VVAEKIQYAE EDLALVAITF SGEKHELQPN
310 320 330 340 350
DLVISKSLEA SGRIYVYRKD LADTLNPFAE NEESQQRSMR ILGMNTWDLA
360 370 380 390 400
LELMNFDWSL FNSIHEQELI YFTFSRQGSG EHTANLSLLL QRCNEVQLWV
410 420 430 440 450
ATEILLCSQL GKRVQLVKKF IKIAAHCKAQ RNLNSFFAIV MGLNTASVSR
460 470 480 490 500
LSQTWEKIPG KFKKLFSELE SLTDPSLNHK AYRDAFKKMK PPKIPFMPLL
510 520 530 540 550
LKDVTFIHEG NKTFLDNLVN FEKLHMIADT VRTLRHCRTN QFGDLSPKEH
560 570 580
QELKSYVNHL YVIDSQQALF ELSHRIEPRV
Length:580
Mass (Da):67,733
Last modified:February 1, 1997 - v1
Checksum:i732FB7AA11DFDA1C
GO
Isoform 2 (identifier: Q92565-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     270-405: Missing.

Note: No experimental confirmation available.
Show »
Length:444
Mass (Da):52,083
Checksum:iF615DF931DCEF02B
GO

Sequence cautioni

The sequence BAA13406 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti497M → V in AAH39203 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_007616270 – 405Missing in isoform 2. 1 PublicationAdd BLAST136

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D87467 mRNA. Translation: BAA13406.2. Different initiation.
CH236948 Genomic DNA. Translation: EAL24270.1.
BC039203 mRNA. Translation: AAH39203.1.
RefSeqiNP_036426.3. NM_012294.3.
UniGeneiHs.174768.
Hs.713217.

Genome annotation databases

EnsembliENST00000401957; ENSP00000384044; ENSG00000136237. [Q92565-1]
ENST00000620335; ENSP00000479340; ENSG00000136237. [Q92565-2]
GeneIDi9771.
KEGGihsa:9771.
UCSCiuc011jym.2. human. [Q92565-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D87467 mRNA. Translation: BAA13406.2. Different initiation.
CH236948 Genomic DNA. Translation: EAL24270.1.
BC039203 mRNA. Translation: AAH39203.1.
RefSeqiNP_036426.3. NM_012294.3.
UniGeneiHs.174768.
Hs.713217.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WGYNMR-A241-331[»]
ProteinModelPortaliQ92565.
SMRiQ92565.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115116. 28 interactors.
IntActiQ92565. 3 interactors.
STRINGi9606.ENSP00000343656.

PTM databases

iPTMnetiQ92565.
PhosphoSitePlusiQ92565.

Polymorphism and mutation databases

DMDMi32171396.

Proteomic databases

MaxQBiQ92565.
PaxDbiQ92565.
PeptideAtlasiQ92565.
PRIDEiQ92565.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000401957; ENSP00000384044; ENSG00000136237. [Q92565-1]
ENST00000620335; ENSP00000479340; ENSG00000136237. [Q92565-2]
GeneIDi9771.
KEGGihsa:9771.
UCSCiuc011jym.2. human. [Q92565-1]

Organism-specific databases

CTDi9771.
DisGeNETi9771.
GeneCardsiRAPGEF5.
H-InvDBHIX0006511.
HGNCiHGNC:16862. RAPGEF5.
HPAiHPA057821.
MIMi609527. gene.
neXtProtiNX_Q92565.
OpenTargetsiENSG00000136237.
PharmGKBiPA134902361.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2378. Eukaryota.
ENOG410XPX9. LUCA.
GeneTreeiENSGT00860000133664.
HOGENOMiHOG000230545.
HOVERGENiHBG056985.
InParanoidiQ92565.
KOiK08019.
PhylomeDBiQ92565.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000136237-MONOMER.
SIGNORiQ92565.

Miscellaneous databases

ChiTaRSiRAPGEF5. human.
EvolutionaryTraceiQ92565.
GeneWikiiRAPGEF5.
GenomeRNAii9771.
PROiQ92565.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000136237.
CleanExiHS_RAPGEF5.
ExpressionAtlasiQ92565. baseline and differential.
GenevisibleiQ92565. HS.

Family and domain databases

CDDicd00155. RasGEF. 1 hit.
cd06224. REM. 1 hit.
Gene3Di1.10.840.10. 1 hit.
InterProiIPR000651. Ras-like_Gua-exchang_fac_N.
IPR019804. Ras_G-nucl-exch_fac_CS.
IPR023578. Ras_GEF_dom.
IPR001895. RASGEF_cat_dom.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF00617. RasGEF. 1 hit.
PF00618. RasGEF_N. 1 hit.
[Graphical view]
SMARTiSM00147. RasGEF. 1 hit.
SM00229. RasGEFN. 1 hit.
[Graphical view]
SUPFAMiSSF48366. SSF48366. 2 hits.
SSF54236. SSF54236. 1 hit.
PROSITEiPS00720. RASGEF. 1 hit.
PS50009. RASGEF_CAT. 1 hit.
PS50212. RASGEF_NTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRPGF5_HUMAN
AccessioniPrimary (citable) accession number: Q92565
Secondary accession number(s): A4D140, Q8IXU5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2003
Last sequence update: February 1, 1997
Last modified: November 30, 2016
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.