Q92563 (TICN2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Testican-2

Alternative name(s):
SPARC/osteonectin, CWCV, and Kazal-like domains proteoglycan 2

Gene names

Name:	SPOCK2
Synonyms:	KIAA0275, TICN2
ORF Names:	UNQ269/PRO306

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	424 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May participate in diverse steps of neurogenesis. Binds calcium.
Subcellular location	Secreted › extracellular space › extracellular matrix Probable.
Tissue specificity	Highly expressed in brain. Also found in lung and testis. Ref.1
Post-translational modification	Contains chondroitin sulfate and heparan sulfate O-linked oligosaccharides By similarity.
Sequence similarities	Contains 1 Kazal-like domain. Contains 1 thyroglobulin type-1 domain.
Sequence caution	The sequence BAA13404.2 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Extracellular matrix Secreted
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Signal
Ligand	Calcium
PTM	Disulfide bond Glycoprotein Heparan sulfate Proteoglycan
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	extracellular matrix organization Non-traceable author statement Ref.1. Source: UniProtKB peptide cross-linking via chondroitin 4-sulfate glycosaminoglycan Inferred from electronic annotation. Source: Compara positive regulation of cell-substrate adhesion Inferred from electronic annotation. Source: Compara regulation of cell differentiation Non-traceable author statement Ref.1. Source: UniProtKB signal transduction Inferred from electronic annotation. Source: InterPro synapse assembly Non-traceable author statement Ref.1. Source: UniProtKB
Cellular_component	proteinaceous extracellular matrix Non-traceable author statement Ref.1. Source: UniProtKB
Molecular_function	calcium ion binding Inferred from direct assay Ref.1. Source: UniProtKB extracellular matrix binding Inferred from electronic annotation. Source: Compara glycosaminoglycan binding Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q92563-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q92563-2)

The sequence of this isoform differs from the canonical sequence as follows:
     64-77: EVEDDYIKSWEDNQ → VPSSDPPSTTQATP
     78-424: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Ref.7
	Chain	23 – 424	402	Testican-2		PRO_0000026701
Regions
	Domain	130 – 182	53	Kazal-like
	Domain	310 – 376	67	Thyroglobulin type-1
	Compositional bias	393 – 416	24	Glu-rich
Amino acid modifications
	Glycosylation	225	1	N-linked (GlcNAc...) Potential
	Glycosylation	383	1	O-linked (Xyl...) (glycosaminoglycan) Potential
	Glycosylation	388	1	O-linked (Xyl...) (glycosaminoglycan) Potential
	Disulfide bond	90 ↔ 101		By similarity
	Disulfide bond	95 ↔ 111		By similarity
	Disulfide bond	136 ↔ 166		By similarity
	Disulfide bond	139 ↔ 159		By similarity
	Disulfide bond	148 ↔ 180		By similarity
	Disulfide bond	313 ↔ 337		By similarity
	Disulfide bond	348 ↔ 355		By similarity
	Disulfide bond	357 ↔ 376		By similarity
Natural variations
	Alternative sequence	64 – 77	14	EVEDD…WEDNQ → VPSSDPPSTTQATP in isoform 2.		VSP_045668
	Alternative sequence	78 – 424	347	Missing in isoform 2.		VSP_045669
	Natural variant	353	1	G → S. Corresponds to variant rs2306322 [ dbSNP | Ensembl ].		VAR_022020
Experimental info
	Sequence conflict	357	1	C → R in AAQ89280. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: B7894E657FC2257F	FASTA	424	46,779
	10 20 30 40 50 60 MRAPGCGRLV LPLLLLAAAA LAEGDAKGLK EGETPGNFME DEQWLSSISQ YSGKIKHWNR 70 80 90 100 110 120 FRDEVEDDYI KSWEDNQQGD EALDTTKDPC QKVKCSRHKV CIAQGYQRAM CISRKKLEHR 130 140 150 160 170 180 IKQPTVKLHG NKDSICKPCH MAQLASVCGS DGHTYSSVCK LEQQACLSSK QLAVRCEGPC 190 200 210 220 230 240 PCPTEQAATS TADGKPETCT GQDLADLGDR LRDWFQLLHE NSKQNGSASS VAGPASGLDK 250 260 270 280 290 300 SLGASCKDSI GWMFSKLDTS ADLFLDQTEL AAINLDKYEV CIRPFFNSCD TYKDGRVSTA 310 320 330 340 350 360 EWCFCFWREK PPCLAELERI QIQEAAKKKP GIFIPSCDED GYYRKMQCDQ SSGDCWCVDQ 370 380 390 400 410 420 LGLELTGTRT HGSPDCDDIV GFSGDFGSGV GWEDEEEKET EEAGEEAEEE EGEAGEADDG GYIW
	Isoform 2 [UniParc]. Checksum: 09ACECE0F615B8CC	FASTA	77	8,311
	10 20 30 40 50 60 MRAPGCGRLV LPLLLLAAAA LAEGDAKGLK EGETPGNFME DEQWLSSISQ YSGKIKHWNR 70 FRDVPSSDPP STTQATP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of testican-2: defining a novel calcium-binding proteoglycan family expressed in brain." Vannahme C., Schuebel S., Herud M., Goesling S., Hulsmann H., Paulsson M., Hartmann U., Maurer P. J. Neurochem. 73:12-20(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, CALCIUM-BINDING. Tissue: Brain.
[2]	"Prediction of the coding sequences of unidentified human genes. VI. The coding sequences of 80 new genes (KIAA0201-KIAA0280) deduced by analysis of cDNA clones from cell line KG-1 and brain." Nagase T., Seki N., Ishikawa K., Ohira M., Kawarabayasi Y., Ohara O., Tanaka A., Kotani H., Miyajima N., Nomura N. DNA Res. 3:321-329(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
[3]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Amygdala.
[5]	"The DNA sequence and comparative analysis of human chromosome 10." Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J. , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J. Nature 429:375-381(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Muscle.
[7]	"Signal peptide prediction based on analysis of experimentally verified cleavage sites." Zhang Z., Henzel W.J. Protein Sci. 13:2819-2824(2004) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 23-37.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ001453 mRNA. Translation: CAA04774.1. D87465 mRNA. Translation: BAA13404.2. Different initiation. AY358921 mRNA. Translation: AAQ89280.1. AK307091 mRNA. No translation available. AC022392 Genomic DNA. No translation available. BC023558 mRNA. Translation: AAH23558.1.
IPI	IPI00006128. IPI00909202.
RefSeq	NP_001127906.1. NM_001134434.1. NP_001231879.1. NM_001244950.1. NP_055582.1. NM_014767.2.
UniGene	Hs.523009.
3D structure databases
ProteinModelPortal	Q92563.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q92563. 2 interactions.
STRING	9606.ENSP00000321108.
Protein family/group databases
MEROPS	I31.954.
Polymorphism databases
DMDM	24212500.
Proteomic databases
PaxDb	Q92563.
PRIDE	Q92563.
Protocols and materials databases
DNASU	9806.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000317376; ENSP00000321108; ENSG00000107742. ENST00000412663; ENSP00000397715; ENSG00000107742.
GeneID	9806.
KEGG	hsa:9806.
UCSC	uc001jso.2. human.
Organism-specific databases
CTD	9806.
GeneCards	GC10M073818.
HGNC	HGNC:13564. SPOCK2.
HPA	HPA044605.
MIM	607988. gene.
neXtProt	NX_Q92563.
PharmGKB	PA128394560.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG244822.
HOGENOM	HOG000293295.
HOVERGEN	HBG026595.
InParanoid	Q92563.
KO	K08136.
OMA	EHRIKQP.
PhylomeDB	Q92563.
Gene expression databases
ArrayExpress	Q92563.
Bgee	Q92563.
CleanEx	HS_SPOCK2.
Genevestigator	Q92563.
GermOnline	ENSG00000107742. Homo sapiens.
Family and domain databases
Gene3D	1.10.238.10. 1 hit. 4.10.800.10. 1 hit.
InterPro	IPR011992. EF-hand-like_dom. IPR002350. Kazal_dom. IPR019577. SPARC/Testican_Ca-bd-dom. IPR000716. Thyroglobulin_1. [Graphical view]
Pfam	PF07648. Kazal_2. 1 hit. PF10591. SPARC_Ca_bdg. 1 hit. PF00086. Thyroglobulin_1. 1 hit. [Graphical view]
SMART	SM00280. KAZAL. 1 hit. SM00211. TY. 1 hit. [Graphical view]
SUPFAM	SSF57610. Thyroglobulin_1. 1 hit.
PROSITE	PS51465. KAZAL_2. 1 hit. PS00484. THYROGLOBULIN_1_1. 1 hit. PS51162. THYROGLOBULIN_1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	9806.
NextBio	36920.
SOURCE	Search...

Entry information

Entry name

TICN2_HUMAN

Accession

Primary (citable) accession number: Q92563
Secondary accession number(s): C9J767, Q6UW87

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 19, 2002
Last sequence update:	February 1, 1997
Last modified:	May 1, 2013
This is version 133 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.