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Protein

Testican-2

Gene

SPOCK2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May participate in diverse steps of neurogenesis. Binds calcium.

GO - Molecular functioni

  1. calcium ion binding Source: UniProtKB
  2. extracellular matrix binding Source: Ensembl
  3. glycosaminoglycan binding Source: Ensembl

GO - Biological processi

  1. extracellular matrix organization Source: UniProtKB
  2. peptide cross-linking via chondroitin 4-sulfate glycosaminoglycan Source: Ensembl
  3. positive regulation of cell-substrate adhesion Source: Ensembl
  4. regulation of cell differentiation Source: UniProtKB
  5. signal transduction Source: InterPro
  6. synapse assembly Source: UniProtKB
Complete GO annotation...

Keywords - Ligandi

Calcium

Protein family/group databases

MEROPSiI31.954.

Names & Taxonomyi

Protein namesi
Recommended name:
Testican-2
Alternative name(s):
SPARC/osteonectin, CWCV, and Kazal-like domains proteoglycan 2
Gene namesi
Name:SPOCK2
Synonyms:KIAA0275, TICN2
ORF Names:UNQ269/PRO306
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 10

Organism-specific databases

HGNCiHGNC:13564. SPOCK2.

Subcellular locationi

GO - Cellular componenti

  1. proteinaceous extracellular matrix Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Extracellular matrix, Secreted

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA128394560.

Polymorphism and mutation databases

BioMutaiSPOCK2.
DMDMi24212500.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 22221 PublicationAdd
BLAST
Chaini23 – 424402Testican-2PRO_0000026701Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi90 ↔ 101By similarity
Disulfide bondi95 ↔ 111By similarity
Disulfide bondi136 ↔ 166By similarity
Disulfide bondi139 ↔ 159By similarity
Disulfide bondi148 ↔ 180By similarity
Glycosylationi225 – 2251N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi313 ↔ 337By similarity
Disulfide bondi348 ↔ 355By similarity
Disulfide bondi357 ↔ 376By similarity
Glycosylationi383 – 3831O-linked (Xyl...) (glycosaminoglycan)Sequence Analysis
Glycosylationi388 – 3881O-linked (Xyl...) (glycosaminoglycan)Sequence Analysis

Post-translational modificationi

Contains chondroitin sulfate and heparan sulfate O-linked oligosaccharides.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Heparan sulfate, Proteoglycan

Proteomic databases

MaxQBiQ92563.
PaxDbiQ92563.
PRIDEiQ92563.

Expressioni

Tissue specificityi

Highly expressed in brain. Also found in lung and testis.1 Publication

Gene expression databases

BgeeiQ92563.
CleanExiHS_SPOCK2.
ExpressionAtlasiQ92563. baseline and differential.
GenevestigatoriQ92563.

Organism-specific databases

HPAiHPA044605.

Interactioni

Protein-protein interaction databases

BioGridi115146. 2 interactions.
IntActiQ92563. 2 interactions.
STRINGi9606.ENSP00000321108.

Structurei

3D structure databases

ProteinModelPortaliQ92563.
SMRiQ92563. Positions 89-306, 330-378.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini130 – 18253Kazal-likePROSITE-ProRule annotationAdd
BLAST
Domaini310 – 37667Thyroglobulin type-1PROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi393 – 41624Glu-richAdd
BLAST

Sequence similaritiesi

Contains 1 Kazal-like domain.PROSITE-ProRule annotation
Contains 1 thyroglobulin type-1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG244822.
GeneTreeiENSGT00510000046429.
HOGENOMiHOG000293295.
HOVERGENiHBG026595.
InParanoidiQ92563.
KOiK08136.
OMAiEHRIKQP.
PhylomeDBiQ92563.
TreeFamiTF317779.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
4.10.800.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR002350. Kazal_dom.
IPR019577. SPARC/Testican_Ca-bd-dom.
IPR000716. Thyroglobulin_1.
[Graphical view]
PfamiPF07648. Kazal_2. 1 hit.
PF10591. SPARC_Ca_bdg. 1 hit.
PF00086. Thyroglobulin_1. 1 hit.
[Graphical view]
SMARTiSM00280. KAZAL. 1 hit.
SM00211. TY. 1 hit.
[Graphical view]
SUPFAMiSSF57610. SSF57610. 1 hit.
PROSITEiPS51465. KAZAL_2. 1 hit.
PS00484. THYROGLOBULIN_1_1. 1 hit.
PS51162. THYROGLOBULIN_1_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q92563-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRAPGCGRLV LPLLLLAAAA LAEGDAKGLK EGETPGNFME DEQWLSSISQ
60 70 80 90 100
YSGKIKHWNR FRDEVEDDYI KSWEDNQQGD EALDTTKDPC QKVKCSRHKV
110 120 130 140 150
CIAQGYQRAM CISRKKLEHR IKQPTVKLHG NKDSICKPCH MAQLASVCGS
160 170 180 190 200
DGHTYSSVCK LEQQACLSSK QLAVRCEGPC PCPTEQAATS TADGKPETCT
210 220 230 240 250
GQDLADLGDR LRDWFQLLHE NSKQNGSASS VAGPASGLDK SLGASCKDSI
260 270 280 290 300
GWMFSKLDTS ADLFLDQTEL AAINLDKYEV CIRPFFNSCD TYKDGRVSTA
310 320 330 340 350
EWCFCFWREK PPCLAELERI QIQEAAKKKP GIFIPSCDED GYYRKMQCDQ
360 370 380 390 400
SSGDCWCVDQ LGLELTGTRT HGSPDCDDIV GFSGDFGSGV GWEDEEEKET
410 420
EEAGEEAEEE EGEAGEADDG GYIW
Length:424
Mass (Da):46,779
Last modified:February 1, 1997 - v1
Checksum:iB7894E657FC2257F
GO
Isoform 2 (identifier: Q92563-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     64-77: EVEDDYIKSWEDNQ → VPSSDPPSTTQATP
     78-424: Missing.

Note: No experimental confirmation available.

Show »
Length:77
Mass (Da):8,311
Checksum:i09ACECE0F615B8CC
GO

Sequence cautioni

The sequence BAA13404.2 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti357 – 3571C → R in AAQ89280 (PubMed:12975309).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti353 – 3531G → S.
Corresponds to variant rs2306322 [ dbSNP | Ensembl ].
VAR_022020

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei64 – 7714EVEDD…WEDNQ → VPSSDPPSTTQATP in isoform 2. 1 PublicationVSP_045668Add
BLAST
Alternative sequencei78 – 424347Missing in isoform 2. 1 PublicationVSP_045669Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ001453 mRNA. Translation: CAA04774.1.
D87465 mRNA. Translation: BAA13404.2. Different initiation.
AY358921 mRNA. Translation: AAQ89280.1.
AK307091 mRNA. No translation available.
AC022392 Genomic DNA. No translation available.
BC023558 mRNA. Translation: AAH23558.1.
CCDSiCCDS44431.1. [Q92563-2]
CCDS7313.1. [Q92563-1]
RefSeqiNP_001127906.1. NM_001134434.1. [Q92563-2]
NP_001231879.1. NM_001244950.1. [Q92563-1]
NP_055582.1. NM_014767.2. [Q92563-1]
UniGeneiHs.523009.

Genome annotation databases

EnsembliENST00000317376; ENSP00000321108; ENSG00000107742. [Q92563-1]
ENST00000373109; ENSP00000362201; ENSG00000107742. [Q92563-1]
ENST00000412663; ENSP00000397715; ENSG00000107742. [Q92563-2]
GeneIDi9806.
KEGGihsa:9806.
UCSCiuc001jso.2. human. [Q92563-1]

Polymorphism and mutation databases

BioMutaiSPOCK2.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ001453 mRNA. Translation: CAA04774.1.
D87465 mRNA. Translation: BAA13404.2. Different initiation.
AY358921 mRNA. Translation: AAQ89280.1.
AK307091 mRNA. No translation available.
AC022392 Genomic DNA. No translation available.
BC023558 mRNA. Translation: AAH23558.1.
CCDSiCCDS44431.1. [Q92563-2]
CCDS7313.1. [Q92563-1]
RefSeqiNP_001127906.1. NM_001134434.1. [Q92563-2]
NP_001231879.1. NM_001244950.1. [Q92563-1]
NP_055582.1. NM_014767.2. [Q92563-1]
UniGeneiHs.523009.

3D structure databases

ProteinModelPortaliQ92563.
SMRiQ92563. Positions 89-306, 330-378.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115146. 2 interactions.
IntActiQ92563. 2 interactions.
STRINGi9606.ENSP00000321108.

Protein family/group databases

MEROPSiI31.954.

Polymorphism and mutation databases

BioMutaiSPOCK2.
DMDMi24212500.

Proteomic databases

MaxQBiQ92563.
PaxDbiQ92563.
PRIDEiQ92563.

Protocols and materials databases

DNASUi9806.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000317376; ENSP00000321108; ENSG00000107742. [Q92563-1]
ENST00000373109; ENSP00000362201; ENSG00000107742. [Q92563-1]
ENST00000412663; ENSP00000397715; ENSG00000107742. [Q92563-2]
GeneIDi9806.
KEGGihsa:9806.
UCSCiuc001jso.2. human. [Q92563-1]

Organism-specific databases

CTDi9806.
GeneCardsiGC10M073818.
HGNCiHGNC:13564. SPOCK2.
HPAiHPA044605.
MIMi607988. gene.
neXtProtiNX_Q92563.
PharmGKBiPA128394560.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG244822.
GeneTreeiENSGT00510000046429.
HOGENOMiHOG000293295.
HOVERGENiHBG026595.
InParanoidiQ92563.
KOiK08136.
OMAiEHRIKQP.
PhylomeDBiQ92563.
TreeFamiTF317779.

Miscellaneous databases

GeneWikiiSPOCK2.
GenomeRNAii9806.
NextBioi36920.
PROiQ92563.
SOURCEiSearch...

Gene expression databases

BgeeiQ92563.
CleanExiHS_SPOCK2.
ExpressionAtlasiQ92563. baseline and differential.
GenevestigatoriQ92563.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
4.10.800.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR002350. Kazal_dom.
IPR019577. SPARC/Testican_Ca-bd-dom.
IPR000716. Thyroglobulin_1.
[Graphical view]
PfamiPF07648. Kazal_2. 1 hit.
PF10591. SPARC_Ca_bdg. 1 hit.
PF00086. Thyroglobulin_1. 1 hit.
[Graphical view]
SMARTiSM00280. KAZAL. 1 hit.
SM00211. TY. 1 hit.
[Graphical view]
SUPFAMiSSF57610. SSF57610. 1 hit.
PROSITEiPS51465. KAZAL_2. 1 hit.
PS00484. THYROGLOBULIN_1_1. 1 hit.
PS51162. THYROGLOBULIN_1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of testican-2: defining a novel calcium-binding proteoglycan family expressed in brain."
    Vannahme C., Schuebel S., Herud M., Goesling S., Hulsmann H., Paulsson M., Hartmann U., Maurer P.
    J. Neurochem. 73:12-20(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, CALCIUM-BINDING.
    Tissue: Brain.
  2. "Prediction of the coding sequences of unidentified human genes. VI. The coding sequences of 80 new genes (KIAA0201-KIAA0280) deduced by analysis of cDNA clones from cell line KG-1 and brain."
    Nagase T., Seki N., Ishikawa K., Ohira M., Kawarabayasi Y., Ohara O., Tanaka A., Kotani H., Miyajima N., Nomura N.
    DNA Res. 3:321-329(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Brain.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  4. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Amygdala.
  5. "The DNA sequence and comparative analysis of human chromosome 10."
    Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J.
    , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J.
    Nature 429:375-381(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Muscle.
  7. "Signal peptide prediction based on analysis of experimentally verified cleavage sites."
    Zhang Z., Henzel W.J.
    Protein Sci. 13:2819-2824(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 23-37.

Entry informationi

Entry nameiTICN2_HUMAN
AccessioniPrimary (citable) accession number: Q92563
Secondary accession number(s): C9J767, Q6UW87
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 19, 2002
Last sequence update: February 1, 1997
Last modified: April 29, 2015
This is version 148 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 10
    Human chromosome 10: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.