Q92558 (WASF1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Wiskott-Aldrich syndrome protein family member 1
Short name=WASP family protein member 1

Alternative name(s):
Protein WAVE-1
Verprolin homology domain-containing protein 1

Gene names

Name:	WASF1
Synonyms:	KIAA0269, SCAR1, WAVE1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	559 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Downstream effector molecule involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Promotes formation of actin filaments. Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex.
Subunit structure	Component of the WAVE1 complex composed of ABI2, CYFIP1 or CYFIP2, BRK1, NCKAP1 and WASF1/WAVE1. Within the complex, a heterdimer containing NCKAP1 and CYFIP1 interacts with a heterotrimer formed by WAVE1, ABI2 and BRK1. CYFIP2 binds to activated RAC1 which causes the complex to dissociate, releasing activated WASF1. The complex can also be activated by NCK1 By similarity. Binds actin and the Arp2/3 complex. Interacts with BAIAP2. Ref.7 Ref.8
Subcellular location	Cytoplasm › cytoskeleton. Note: Dot-like pattern in the cytoplasm. Concentrated in Rac-regulated membrane-ruffling areas.
Tissue specificity	Highly expressed in brain. Lowly expressed in testis, ovary, colon, kidney, pancreas, thymus, small intestine and peripheral blood.
Domain	Binds the Arp2/3 complex through the C-terminal region and actin through verprolin homology (VPH) domain.
Sequence similarities	Belongs to the SCAR/WAVE family. Contains 1 WH2 domain.
Sequence caution	The sequence BAA13399.2 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton
Ligand	Actin-binding
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	actin filament polymerization Traceable author statement PubMed 9843499. Source: ProtInc cellular component movement Traceable author statement PubMed 2775266. Source: ProtInc
Cellular_component	actin cytoskeleton Traceable author statement PubMed 9843499. Source: ProtInc lamellipodium Inferred from electronic annotation. Source: Compara mitochondrial outer membrane Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
PFN1	P07737	2	EBI-1548747,EBI-713780

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 559

559

Wiskott-Aldrich syndrome protein family member 1

PRO_0000188992

Regions

Domain

497 – 514

18

WH2

Compositional bias

278 – 283

6

Poly-Pro

Compositional bias

322 – 332

11

Poly-Pro

Compositional bias

348 – 359

12

Poly-Pro

Compositional bias

369 – 374

6

Poly-Pro

Compositional bias

424 – 435

12

Poly-Pro

Secondary structure

1

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559

Helix Strand Turn

Details...

Helix

26 – 81

56

Turn

85 – 87

3

Helix

93 – 96

4

Helix

114 – 116

3

Helix

119 – 127

9

Helix

134 – 140

7

Helix

147 – 150

4

Helix

156 – 181

26

Helix

500 – 513

14

Helix

531 – 540

10

Turn

541 – 543

3

Sequences

	Sequence		Length	Mass (Da)
	Q92558 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 44B4527BDB77BC6E	FASTA	559	61,652
	10 20 30 40 50 60 MPLVKRNIDP RHLCHTALPR GIKNELECVT NISLANIIRQ LSSLSKYAED IFGELFNEAH 70 80 90 100 110 120 SFSFRVNSLQ ERVDRLSVSV TQLDPKEEEL SLQDITMRKA FRSSTIQDQQ LFDRKTLPIP 130 140 150 160 170 180 LQETYDVCEQ PPPLNILTPY RDDGKEGLKF YTNPSYFFDL WKEKMLQDTE DKRKEKRKQK 190 200 210 220 230 240 QKNLDRPHEP EKVPRAPHDR RREWQKLAQG PELAEDDANL LHKHIEVANG PASHFETRPQ 250 260 270 280 290 300 TYVDHMDGSY SLSALPFSQM SELLTRAEER VLVRPHEPPP PPPMHGAGDA KPIPTCISSA 310 320 330 340 350 360 TGLIENRPQS PATGRTPVFV SPTPPPPPPP LPSALSTSSL RASMTSTPPP PVPPPPPPPA 370 380 390 400 410 420 TALQAPAVPP PPAPLQIAPG VLHPAPPPIA PPLVQPSPPV ARAAPVCETV PVHPLPQGEV 430 440 450 460 470 480 QGLPPPPPPP PLPPPGIRPS SPVTVTALAH PPSGLHPTPS TAPGPHVPLM PPSPPSQVIP 490 500 510 520 530 540 ASEPKRHPST LPVISDARSV LLEAIRKGIQ LRKVEEQREQ EAKHERIEND VATILSRRIA 550 VEYSDSEDDS EFDEVDWLE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"SCAR, a WASP-related protein, isolated as a suppressor of receptor defects in late Dictyostelium development." Bear J.E., Rawls J.F., Saxe C.L. III J. Cell Biol. 142:1325-1335(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Prediction of the coding sequences of unidentified human genes. VI. The coding sequences of 80 new genes (KIAA0201-KIAA0280) deduced by analysis of cDNA clones from cell line KG-1 and brain." Nagase T., Seki N., Ishikawa K., Ohira M., Kawarabayasi Y., Ohara O., Tanaka A., Kotani H., Miyajima N., Nomura N. DNA Res. 3:321-329(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Bone marrow.
[3]	"The DNA sequence and analysis of human chromosome 6." Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S. Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
[6]	"Scar1 and the related Wiskott-Aldrich syndrome protein, WASP, regulate the actin cytoskeleton through the Arp2/3 complex." Machesky L.M., Insall R.H. Curr. Biol. 8:1347-1356(1998) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
[7]	"IRSp53 is an essential intermediate between Rac and WAVE in the regulation of membrane ruffling." Miki H., Yamaguchi H., Suetsugu S., Takenawa T. Nature 408:732-735(2000) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH BAIAP2.
[8]	"Structure and control of the actin regulatory WAVE complex." Chen Z., Borek D., Padrick S.B., Gomez T.S., Metlagel Z., Ismail A.M., Umetani J., Billadeau D.D., Otwinowski Z., Rosen M.K. Nature 468:533-538(2010) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.29 ANGSTROMS) OF WAVE1 COMPLEX, SUBUNIT.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF134303 mRNA. Translation: AAD33052.1.
D87459 mRNA. Translation: BAA13399.2. Different initiation.
AL590009 Genomic DNA. Translation: CAI12485.1.
CH471051 Genomic DNA. Translation: EAW48325.1.
CH471051 Genomic DNA. Translation: EAW48326.1.
CH471051 Genomic DNA. Translation: EAW48327.1.
CH471051 Genomic DNA. Translation: EAW48328.1.
CH471051 Genomic DNA. Translation: EAW48329.1.
BC044591 mRNA. Translation: AAH44591.1.

IPI

IPI00022007.

RefSeq

NP_001020105.1. NM_001024934.1.
NP_001020106.1. NM_001024935.1.
NP_001020107.1. NM_001024936.1.
NP_003922.1. NM_003931.2.

UniGene

Hs.75850.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3P8C	X-ray	2.29	D	1-559	[»]

ProteinModelPortal

Q92558.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-39391N.

IntAct

Q92558. 9 interactions.

MINT

MINT-144109.

STRING

9606.ENSP00000352425.

PTM databases

PhosphoSite

Q92558.

Polymorphism databases

DMDM

2495730.

Proteomic databases

PaxDb

Q92558.

PRIDE

Q92558.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000359451; ENSP00000352425; ENSG00000112290.
ENST00000392586; ENSP00000376365; ENSG00000112290.
ENST00000392587; ENSP00000376366; ENSG00000112290.
ENST00000392588; ENSP00000376367; ENSG00000112290.
ENST00000392589; ENSP00000376368; ENSG00000112290.

GeneID

8936.

KEGG

hsa:8936.

UCSC

uc003ptv.1. human.

Organism-specific databases

CTD

8936.

GeneCards

GC06M110421.

HGNC

HGNC:12732. WASF1.

HPA

CAB022161.
HPA004105.

MIM

605035. gene.

neXtProt

NX_Q92558.

PharmGKB

PA37343.

HUGE

Search...

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG299696.

HOGENOM

HOG000021456.

HOVERGEN

HBG058482.

InParanoid

Q92558.

KO

K05753.

OMA

HERVGND.

OrthoDB

EOG4C2H9X.

PhylomeDB

Q92558.

Gene expression databases

ArrayExpress

Q92558.

Bgee

Q92558.

CleanEx

HS_WASF1.

Genevestigator

Q92558.

GermOnline

ENSG00000112290. Homo sapiens.

Family and domain databases

InterPro

IPR003124. WH2_dom.
[Graphical view]

Pfam

PF02205. WH2. 1 hit.
[Graphical view]

SMART

SM00246. WH2. 1 hit.
[Graphical view]

PROSITE

PS51082. WH2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

WASF1. human.

EvolutionaryTrace

Q92558.

GenomeRNAi

8936.

NextBio

33600.

PMAP-CutDB

Q92558.

SOURCE

Search...

Entry information

Entry name

WASF1_HUMAN

Accession

Primary (citable) accession number: Q92558
Secondary accession number(s): E1P5F2, Q5SZK7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	May 1, 2013
This is version 104 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.