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Protein

Wiskott-Aldrich syndrome protein family member 1

Gene

WASF1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Downstream effector molecule involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Promotes formation of actin filaments. Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex.

GO - Molecular functioni

  • protein complex binding Source: UniProtKB

GO - Biological processi

  • actin filament polymerization Source: ProtInc
  • lamellipodium morphogenesis Source: Ensembl
  • movement of cell or subcellular component Source: ProtInc
  • positive regulation of Arp2/3 complex-mediated actin nucleation Source: UniProtKB
  • protein complex assembly Source: ProtInc
  • Rac protein signal transduction Source: UniProtKB
Complete GO annotation...

Keywords - Ligandi

Actin-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000112290-MONOMER.
ReactomeiR-HSA-2029482. Regulation of actin dynamics for phagocytic cup formation.
R-HSA-4420097. VEGFA-VEGFR2 Pathway.
R-HSA-5663213. RHO GTPases Activate WASPs and WAVEs.
SIGNORiQ92558.

Names & Taxonomyi

Protein namesi
Recommended name:
Wiskott-Aldrich syndrome protein family member 1
Short name:
WASP family protein member 1
Alternative name(s):
Protein WAVE-1
Verprolin homology domain-containing protein 1
Gene namesi
Name:WASF1
Synonyms:KIAA0269, SCAR1, WAVE1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

HGNCiHGNC:12732. WASF1.

Subcellular locationi

GO - Cellular componenti

  • actin cytoskeleton Source: ProtInc
  • cytoskeleton Source: ProtInc
  • focal adhesion Source: UniProtKB-SubCell
  • lamellipodium Source: Ensembl
  • mitochondrial outer membrane Source: Ensembl
  • SCAR complex Source: UniProtKB
  • synapse Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cytoplasm, Cytoskeleton, Synapse

Pathology & Biotechi

Organism-specific databases

DisGeNETi8936.
OpenTargetsiENSG00000112290.
PharmGKBiPA37343.

Polymorphism and mutation databases

BioMutaiWASF1.
DMDMi2495730.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001889921 – 559Wiskott-Aldrich syndrome protein family member 1Add BLAST559

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei341Asymmetric dimethylarginine; alternateBy similarity1
Modified residuei341Omega-N-methylarginine; alternateBy similarity1
Modified residuei489PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated on tyrosine residues by ABL1 and dephosphorylated by PTPN12.1 Publication

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

EPDiQ92558.
MaxQBiQ92558.
PaxDbiQ92558.
PeptideAtlasiQ92558.
PRIDEiQ92558.

PTM databases

iPTMnetiQ92558.
PhosphoSitePlusiQ92558.

Miscellaneous databases

PMAP-CutDBQ92558.

Expressioni

Tissue specificityi

Highly expressed in brain. Lowly expressed in testis, ovary, colon, kidney, pancreas, thymus, small intestine and peripheral blood.

Gene expression databases

BgeeiENSG00000112290.
CleanExiHS_WASF1.
ExpressionAtlasiQ92558. baseline and differential.
GenevisibleiQ92558. HS.

Organism-specific databases

HPAiCAB022161.
HPA004105.

Interactioni

Subunit structurei

Component of the WAVE1 complex composed of ABI2, CYFIP1 or CYFIP2, BRK1, NCKAP1 and WASF1/WAVE1. Within the complex, a heterodimer containing NCKAP1 and CYFIP1 interacts with a heterotrimer formed by WAVE1, ABI2 and BRK1. CYFIP2 binds to activated RAC1 which causes the complex to dissociate, releasing activated WASF1. The complex can also be activated by NCK1. Binds actin and the Arp2/3 complex. Interacts with BAIAP2. Interacts with SHANK3; the interaction mediates the association of SHANK3 with the WAVE1 complex. Interacts with ABI1 (via N-terminus). Interacts with SORBS2; this interaction greatly enhances phosphorylation by ABL1 and dephosphorylation by PTPN12 and might mediate partial to focal adhesion sites.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
PFN1P077372EBI-1548747,EBI-713780
TRIP10Q156423EBI-1548747,EBI-739936

GO - Molecular functioni

  • protein complex binding Source: UniProtKB

Protein-protein interaction databases

BioGridi114449. 36 interactors.
DIPiDIP-39391N.
IntActiQ92558. 27 interactors.
MINTiMINT-144109.
STRINGi9606.ENSP00000352425.

Structurei

Secondary structure

1559
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi26 – 81Combined sources56
Turni85 – 87Combined sources3
Helixi93 – 96Combined sources4
Helixi114 – 116Combined sources3
Helixi119 – 127Combined sources9
Helixi134 – 140Combined sources7
Helixi147 – 150Combined sources4
Helixi156 – 181Combined sources26
Helixi500 – 513Combined sources14
Helixi531 – 540Combined sources10
Turni541 – 543Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3P8CX-ray2.29D1-186[»]
D485-559[»]
4N78X-ray2.43D1-559[»]
ProteinModelPortaliQ92558.
SMRiQ92558.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ92558.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini497 – 514WH2PROSITE-ProRule annotationAdd BLAST18

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi278 – 283Poly-Pro6
Compositional biasi322 – 332Poly-ProAdd BLAST11
Compositional biasi348 – 359Poly-ProAdd BLAST12
Compositional biasi369 – 374Poly-Pro6
Compositional biasi424 – 435Poly-ProAdd BLAST12

Domaini

Binds the Arp2/3 complex through the C-terminal region and actin through verprolin homology (VPH) domain.

Sequence similaritiesi

Belongs to the SCAR/WAVE family.Curated
Contains 1 WH2 domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1830. Eukaryota.
ENOG410XRBQ. LUCA.
GeneTreeiENSGT00730000110593.
HOGENOMiHOG000021456.
HOVERGENiHBG058482.
InParanoidiQ92558.
KOiK05753.
OMAiVKPIPTC.
OrthoDBiEOG091G08O1.
PhylomeDBiQ92558.
TreeFamiTF315031.

Family and domain databases

InterProiIPR028288. SCAR/WAVE_fam.
IPR003124. WH2_dom.
[Graphical view]
PANTHERiPTHR12902. PTHR12902. 1 hit.
PfamiPF02205. WH2. 1 hit.
[Graphical view]
SMARTiSM00246. WH2. 1 hit.
[Graphical view]
PROSITEiPS51082. WH2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q92558-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPLVKRNIDP RHLCHTALPR GIKNELECVT NISLANIIRQ LSSLSKYAED
60 70 80 90 100
IFGELFNEAH SFSFRVNSLQ ERVDRLSVSV TQLDPKEEEL SLQDITMRKA
110 120 130 140 150
FRSSTIQDQQ LFDRKTLPIP LQETYDVCEQ PPPLNILTPY RDDGKEGLKF
160 170 180 190 200
YTNPSYFFDL WKEKMLQDTE DKRKEKRKQK QKNLDRPHEP EKVPRAPHDR
210 220 230 240 250
RREWQKLAQG PELAEDDANL LHKHIEVANG PASHFETRPQ TYVDHMDGSY
260 270 280 290 300
SLSALPFSQM SELLTRAEER VLVRPHEPPP PPPMHGAGDA KPIPTCISSA
310 320 330 340 350
TGLIENRPQS PATGRTPVFV SPTPPPPPPP LPSALSTSSL RASMTSTPPP
360 370 380 390 400
PVPPPPPPPA TALQAPAVPP PPAPLQIAPG VLHPAPPPIA PPLVQPSPPV
410 420 430 440 450
ARAAPVCETV PVHPLPQGEV QGLPPPPPPP PLPPPGIRPS SPVTVTALAH
460 470 480 490 500
PPSGLHPTPS TAPGPHVPLM PPSPPSQVIP ASEPKRHPST LPVISDARSV
510 520 530 540 550
LLEAIRKGIQ LRKVEEQREQ EAKHERIEND VATILSRRIA VEYSDSEDDS

EFDEVDWLE
Length:559
Mass (Da):61,652
Last modified:February 1, 1997 - v1
Checksum:i44B4527BDB77BC6E
GO

Sequence cautioni

The sequence BAA13399 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF134303 mRNA. Translation: AAD33052.1.
D87459 mRNA. Translation: BAA13399.2. Different initiation.
AL590009 Genomic DNA. Translation: CAI12485.1.
CH471051 Genomic DNA. Translation: EAW48325.1.
CH471051 Genomic DNA. Translation: EAW48326.1.
CH471051 Genomic DNA. Translation: EAW48327.1.
CH471051 Genomic DNA. Translation: EAW48328.1.
CH471051 Genomic DNA. Translation: EAW48329.1.
BC044591 mRNA. Translation: AAH44591.1.
CCDSiCCDS5080.1.
RefSeqiNP_001020105.1. NM_001024934.1.
NP_001020106.1. NM_001024935.1.
NP_001020107.1. NM_001024936.1.
NP_003922.1. NM_003931.2.
XP_005267260.1. XM_005267203.3.
XP_005267262.1. XM_005267205.2.
XP_005267263.1. XM_005267206.2.
XP_005267264.1. XM_005267207.2.
XP_011534535.1. XM_011536233.1.
XP_011534536.1. XM_011536234.1.
XP_016866926.1. XM_017011437.1.
XP_016866927.1. XM_017011438.1.
UniGeneiHs.75850.

Genome annotation databases

EnsembliENST00000359451; ENSP00000352425; ENSG00000112290.
ENST00000392586; ENSP00000376365; ENSG00000112290.
ENST00000392587; ENSP00000376366; ENSG00000112290.
ENST00000392588; ENSP00000376367; ENSG00000112290.
ENST00000392589; ENSP00000376368; ENSG00000112290.
GeneIDi8936.
KEGGihsa:8936.
UCSCiuc003ptv.1. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF134303 mRNA. Translation: AAD33052.1.
D87459 mRNA. Translation: BAA13399.2. Different initiation.
AL590009 Genomic DNA. Translation: CAI12485.1.
CH471051 Genomic DNA. Translation: EAW48325.1.
CH471051 Genomic DNA. Translation: EAW48326.1.
CH471051 Genomic DNA. Translation: EAW48327.1.
CH471051 Genomic DNA. Translation: EAW48328.1.
CH471051 Genomic DNA. Translation: EAW48329.1.
BC044591 mRNA. Translation: AAH44591.1.
CCDSiCCDS5080.1.
RefSeqiNP_001020105.1. NM_001024934.1.
NP_001020106.1. NM_001024935.1.
NP_001020107.1. NM_001024936.1.
NP_003922.1. NM_003931.2.
XP_005267260.1. XM_005267203.3.
XP_005267262.1. XM_005267205.2.
XP_005267263.1. XM_005267206.2.
XP_005267264.1. XM_005267207.2.
XP_011534535.1. XM_011536233.1.
XP_011534536.1. XM_011536234.1.
XP_016866926.1. XM_017011437.1.
XP_016866927.1. XM_017011438.1.
UniGeneiHs.75850.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3P8CX-ray2.29D1-186[»]
D485-559[»]
4N78X-ray2.43D1-559[»]
ProteinModelPortaliQ92558.
SMRiQ92558.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114449. 36 interactors.
DIPiDIP-39391N.
IntActiQ92558. 27 interactors.
MINTiMINT-144109.
STRINGi9606.ENSP00000352425.

PTM databases

iPTMnetiQ92558.
PhosphoSitePlusiQ92558.

Polymorphism and mutation databases

BioMutaiWASF1.
DMDMi2495730.

Proteomic databases

EPDiQ92558.
MaxQBiQ92558.
PaxDbiQ92558.
PeptideAtlasiQ92558.
PRIDEiQ92558.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000359451; ENSP00000352425; ENSG00000112290.
ENST00000392586; ENSP00000376365; ENSG00000112290.
ENST00000392587; ENSP00000376366; ENSG00000112290.
ENST00000392588; ENSP00000376367; ENSG00000112290.
ENST00000392589; ENSP00000376368; ENSG00000112290.
GeneIDi8936.
KEGGihsa:8936.
UCSCiuc003ptv.1. human.

Organism-specific databases

CTDi8936.
DisGeNETi8936.
GeneCardsiWASF1.
HGNCiHGNC:12732. WASF1.
HPAiCAB022161.
HPA004105.
MIMi605035. gene.
neXtProtiNX_Q92558.
OpenTargetsiENSG00000112290.
PharmGKBiPA37343.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1830. Eukaryota.
ENOG410XRBQ. LUCA.
GeneTreeiENSGT00730000110593.
HOGENOMiHOG000021456.
HOVERGENiHBG058482.
InParanoidiQ92558.
KOiK05753.
OMAiVKPIPTC.
OrthoDBiEOG091G08O1.
PhylomeDBiQ92558.
TreeFamiTF315031.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000112290-MONOMER.
ReactomeiR-HSA-2029482. Regulation of actin dynamics for phagocytic cup formation.
R-HSA-4420097. VEGFA-VEGFR2 Pathway.
R-HSA-5663213. RHO GTPases Activate WASPs and WAVEs.
SIGNORiQ92558.

Miscellaneous databases

ChiTaRSiWASF1. human.
EvolutionaryTraceiQ92558.
GeneWikiiWASF1.
GenomeRNAii8936.
PMAP-CutDBQ92558.
PROiQ92558.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000112290.
CleanExiHS_WASF1.
ExpressionAtlasiQ92558. baseline and differential.
GenevisibleiQ92558. HS.

Family and domain databases

InterProiIPR028288. SCAR/WAVE_fam.
IPR003124. WH2_dom.
[Graphical view]
PANTHERiPTHR12902. PTHR12902. 1 hit.
PfamiPF02205. WH2. 1 hit.
[Graphical view]
SMARTiSM00246. WH2. 1 hit.
[Graphical view]
PROSITEiPS51082. WH2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiWASF1_HUMAN
AccessioniPrimary (citable) accession number: Q92558
Secondary accession number(s): E1P5F2, Q5SZK7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: November 30, 2016
This is version 141 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.