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Q92558

- WASF1_HUMAN

UniProt

Q92558 - WASF1_HUMAN

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Protein

Wiskott-Aldrich syndrome protein family member 1

Gene
WASF1, KIAA0269, SCAR1, WAVE1
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Downstream effector molecule involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Promotes formation of actin filaments. Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex.

GO - Molecular functioni

  1. protein binding Source: IntAct
  2. protein complex binding Source: UniProt

GO - Biological processi

  1. actin filament polymerization Source: ProtInc
  2. cellular component movement Source: ProtInc
  3. lamellipodium morphogenesis Source: Ensembl
  4. positive regulation of Arp2/3 complex-mediated actin nucleation Source: UniProt
  5. protein complex assembly Source: ProtInc
  6. Rac protein signal transduction Source: UniProt
Complete GO annotation...

Keywords - Ligandi

Actin-binding

Enzyme and pathway databases

ReactomeiREACT_160086. Regulation of actin dynamics for phagocytic cup formation.

Names & Taxonomyi

Protein namesi
Recommended name:
Wiskott-Aldrich syndrome protein family member 1
Short name:
WASP family protein member 1
Alternative name(s):
Protein WAVE-1
Verprolin homology domain-containing protein 1
Gene namesi
Name:WASF1
Synonyms:KIAA0269, SCAR1, WAVE1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 6

Organism-specific databases

HGNCiHGNC:12732. WASF1.

Subcellular locationi

Cytoplasmcytoskeleton. Cell junctionsynapse By similarity
Note: Dot-like pattern in the cytoplasm. Concentrated in Rac-regulated membrane-ruffling areas.

GO - Cellular componenti

  1. actin cytoskeleton Source: ProtInc
  2. cell junction Source: UniProtKB-KW
  3. cytoskeleton Source: ProtInc
  4. lamellipodium Source: Ensembl
  5. mitochondrial outer membrane Source: Ensembl
  6. SCAR complex Source: UniProt
  7. synapse Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cytoplasm, Cytoskeleton, Synapse

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA37343.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 559559Wiskott-Aldrich syndrome protein family member 1PRO_0000188992Add
BLAST

Proteomic databases

MaxQBiQ92558.
PaxDbiQ92558.
PRIDEiQ92558.

PTM databases

PhosphoSiteiQ92558.

Miscellaneous databases

PMAP-CutDBQ92558.

Expressioni

Tissue specificityi

Highly expressed in brain. Lowly expressed in testis, ovary, colon, kidney, pancreas, thymus, small intestine and peripheral blood.

Gene expression databases

ArrayExpressiQ92558.
BgeeiQ92558.
CleanExiHS_WASF1.
GenevestigatoriQ92558.

Organism-specific databases

HPAiCAB022161.
HPA004105.

Interactioni

Subunit structurei

Component of the WAVE1 complex composed of ABI2, CYFIP1 or CYFIP2, BRK1, NCKAP1 and WASF1/WAVE1. Within the complex, a heterdimer containing NCKAP1 and CYFIP1 interacts with a heterotrimer formed by WAVE1, ABI2 and BRK1. CYFIP2 binds to activated RAC1 which causes the complex to dissociate, releasing activated WASF1. The complex can also be activated by NCK1. Binds actin and the Arp2/3 complex. Interacts with BAIAP2. Interacts with SHANK3; the interaction mediates the association of SHANK3 with the WAVE1 complex. Interacts with ABI1 (via N-terminus).2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
PFN1P077372EBI-1548747,EBI-713780

Protein-protein interaction databases

BioGridi114449. 14 interactions.
DIPiDIP-39391N.
IntActiQ92558. 18 interactions.
MINTiMINT-144109.
STRINGi9606.ENSP00000352425.

Structurei

Secondary structure

1
559
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi26 – 8156
Turni85 – 873
Helixi93 – 964
Helixi114 – 1163
Helixi119 – 1279
Helixi134 – 1407
Helixi147 – 1504
Helixi156 – 18126
Helixi500 – 51314
Helixi531 – 54010
Turni541 – 5433

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3P8CX-ray2.29D1-186[»]
D485-559[»]
4N78X-ray2.43D1-559[»]
ProteinModelPortaliQ92558.
SMRiQ92558. Positions 21-227, 496-544.

Miscellaneous databases

EvolutionaryTraceiQ92558.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini497 – 51418WH2Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi278 – 2836Poly-Pro
Compositional biasi322 – 33211Poly-ProAdd
BLAST
Compositional biasi348 – 35912Poly-ProAdd
BLAST
Compositional biasi369 – 3746Poly-Pro
Compositional biasi424 – 43512Poly-ProAdd
BLAST

Domaini

Binds the Arp2/3 complex through the C-terminal region and actin through verprolin homology (VPH) domain.

Sequence similaritiesi

Belongs to the SCAR/WAVE family.
Contains 1 WH2 domain.

Phylogenomic databases

eggNOGiNOG299696.
HOGENOMiHOG000021456.
HOVERGENiHBG058482.
InParanoidiQ92558.
KOiK05753.
OMAiYMEHLDG.
OrthoDBiEOG7VHSXK.
PhylomeDBiQ92558.
TreeFamiTF315031.

Family and domain databases

InterProiIPR028288. SCAR/WAVE_fam.
IPR003124. WH2_dom.
[Graphical view]
PANTHERiPTHR12902. PTHR12902. 1 hit.
PfamiPF02205. WH2. 1 hit.
[Graphical view]
SMARTiSM00246. WH2. 1 hit.
[Graphical view]
PROSITEiPS51082. WH2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q92558-1 [UniParc]FASTAAdd to Basket

« Hide

MPLVKRNIDP RHLCHTALPR GIKNELECVT NISLANIIRQ LSSLSKYAED    50
IFGELFNEAH SFSFRVNSLQ ERVDRLSVSV TQLDPKEEEL SLQDITMRKA 100
FRSSTIQDQQ LFDRKTLPIP LQETYDVCEQ PPPLNILTPY RDDGKEGLKF 150
YTNPSYFFDL WKEKMLQDTE DKRKEKRKQK QKNLDRPHEP EKVPRAPHDR 200
RREWQKLAQG PELAEDDANL LHKHIEVANG PASHFETRPQ TYVDHMDGSY 250
SLSALPFSQM SELLTRAEER VLVRPHEPPP PPPMHGAGDA KPIPTCISSA 300
TGLIENRPQS PATGRTPVFV SPTPPPPPPP LPSALSTSSL RASMTSTPPP 350
PVPPPPPPPA TALQAPAVPP PPAPLQIAPG VLHPAPPPIA PPLVQPSPPV 400
ARAAPVCETV PVHPLPQGEV QGLPPPPPPP PLPPPGIRPS SPVTVTALAH 450
PPSGLHPTPS TAPGPHVPLM PPSPPSQVIP ASEPKRHPST LPVISDARSV 500
LLEAIRKGIQ LRKVEEQREQ EAKHERIEND VATILSRRIA VEYSDSEDDS 550
EFDEVDWLE 559
Length:559
Mass (Da):61,652
Last modified:February 1, 1997 - v1
Checksum:i44B4527BDB77BC6E
GO

Sequence cautioni

The sequence BAA13399.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF134303 mRNA. Translation: AAD33052.1.
D87459 mRNA. Translation: BAA13399.2. Different initiation.
AL590009 Genomic DNA. Translation: CAI12485.1.
CH471051 Genomic DNA. Translation: EAW48325.1.
CH471051 Genomic DNA. Translation: EAW48326.1.
CH471051 Genomic DNA. Translation: EAW48327.1.
CH471051 Genomic DNA. Translation: EAW48328.1.
CH471051 Genomic DNA. Translation: EAW48329.1.
BC044591 mRNA. Translation: AAH44591.1.
CCDSiCCDS5080.1.
RefSeqiNP_001020105.1. NM_001024934.1.
NP_001020106.1. NM_001024935.1.
NP_001020107.1. NM_001024936.1.
NP_003922.1. NM_003931.2.
XP_005267260.1. XM_005267203.2.
XP_005267261.1. XM_005267204.1.
XP_005267262.1. XM_005267205.1.
XP_005267263.1. XM_005267206.1.
XP_005267264.1. XM_005267207.1.
XP_006715658.1. XM_006715595.1.
UniGeneiHs.75850.

Genome annotation databases

EnsembliENST00000359451; ENSP00000352425; ENSG00000112290.
ENST00000392586; ENSP00000376365; ENSG00000112290.
ENST00000392587; ENSP00000376366; ENSG00000112290.
ENST00000392588; ENSP00000376367; ENSG00000112290.
ENST00000392589; ENSP00000376368; ENSG00000112290.
GeneIDi8936.
KEGGihsa:8936.
UCSCiuc003ptv.1. human.

Polymorphism databases

DMDMi2495730.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF134303 mRNA. Translation: AAD33052.1 .
D87459 mRNA. Translation: BAA13399.2 . Different initiation.
AL590009 Genomic DNA. Translation: CAI12485.1 .
CH471051 Genomic DNA. Translation: EAW48325.1 .
CH471051 Genomic DNA. Translation: EAW48326.1 .
CH471051 Genomic DNA. Translation: EAW48327.1 .
CH471051 Genomic DNA. Translation: EAW48328.1 .
CH471051 Genomic DNA. Translation: EAW48329.1 .
BC044591 mRNA. Translation: AAH44591.1 .
CCDSi CCDS5080.1.
RefSeqi NP_001020105.1. NM_001024934.1.
NP_001020106.1. NM_001024935.1.
NP_001020107.1. NM_001024936.1.
NP_003922.1. NM_003931.2.
XP_005267260.1. XM_005267203.2.
XP_005267261.1. XM_005267204.1.
XP_005267262.1. XM_005267205.1.
XP_005267263.1. XM_005267206.1.
XP_005267264.1. XM_005267207.1.
XP_006715658.1. XM_006715595.1.
UniGenei Hs.75850.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3P8C X-ray 2.29 D 1-186 [» ]
D 485-559 [» ]
4N78 X-ray 2.43 D 1-559 [» ]
ProteinModelPortali Q92558.
SMRi Q92558. Positions 21-227, 496-544.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 114449. 14 interactions.
DIPi DIP-39391N.
IntActi Q92558. 18 interactions.
MINTi MINT-144109.
STRINGi 9606.ENSP00000352425.

PTM databases

PhosphoSitei Q92558.

Polymorphism databases

DMDMi 2495730.

Proteomic databases

MaxQBi Q92558.
PaxDbi Q92558.
PRIDEi Q92558.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000359451 ; ENSP00000352425 ; ENSG00000112290 .
ENST00000392586 ; ENSP00000376365 ; ENSG00000112290 .
ENST00000392587 ; ENSP00000376366 ; ENSG00000112290 .
ENST00000392588 ; ENSP00000376367 ; ENSG00000112290 .
ENST00000392589 ; ENSP00000376368 ; ENSG00000112290 .
GeneIDi 8936.
KEGGi hsa:8936.
UCSCi uc003ptv.1. human.

Organism-specific databases

CTDi 8936.
GeneCardsi GC06M110421.
HGNCi HGNC:12732. WASF1.
HPAi CAB022161.
HPA004105.
MIMi 605035. gene.
neXtProti NX_Q92558.
PharmGKBi PA37343.
HUGEi Search...
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG299696.
HOGENOMi HOG000021456.
HOVERGENi HBG058482.
InParanoidi Q92558.
KOi K05753.
OMAi YMEHLDG.
OrthoDBi EOG7VHSXK.
PhylomeDBi Q92558.
TreeFami TF315031.

Enzyme and pathway databases

Reactomei REACT_160086. Regulation of actin dynamics for phagocytic cup formation.

Miscellaneous databases

ChiTaRSi WASF1. human.
EvolutionaryTracei Q92558.
GeneWikii WASF1.
GenomeRNAii 8936.
NextBioi 33600.
PMAP-CutDB Q92558.
PROi Q92558.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q92558.
Bgeei Q92558.
CleanExi HS_WASF1.
Genevestigatori Q92558.

Family and domain databases

InterProi IPR028288. SCAR/WAVE_fam.
IPR003124. WH2_dom.
[Graphical view ]
PANTHERi PTHR12902. PTHR12902. 1 hit.
Pfami PF02205. WH2. 1 hit.
[Graphical view ]
SMARTi SM00246. WH2. 1 hit.
[Graphical view ]
PROSITEi PS51082. WH2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "SCAR, a WASP-related protein, isolated as a suppressor of receptor defects in late Dictyostelium development."
    Bear J.E., Rawls J.F., Saxe C.L. III
    J. Cell Biol. 142:1325-1335(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Prediction of the coding sequences of unidentified human genes. VI. The coding sequences of 80 new genes (KIAA0201-KIAA0280) deduced by analysis of cDNA clones from cell line KG-1 and brain."
    Nagase T., Seki N., Ishikawa K., Ohira M., Kawarabayasi Y., Ohara O., Tanaka A., Kotani H., Miyajima N., Nomura N.
    DNA Res. 3:321-329(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Bone marrow.
  3. "The DNA sequence and analysis of human chromosome 6."
    Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D.
    , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S.
    Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.
  6. "Scar1 and the related Wiskott-Aldrich syndrome protein, WASP, regulate the actin cytoskeleton through the Arp2/3 complex."
    Machesky L.M., Insall R.H.
    Curr. Biol. 8:1347-1356(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  7. "IRSp53 is an essential intermediate between Rac and WAVE in the regulation of membrane ruffling."
    Miki H., Yamaguchi H., Suetsugu S., Takenawa T.
    Nature 408:732-735(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH BAIAP2.
  8. Cited for: X-RAY CRYSTALLOGRAPHY (2.29 ANGSTROMS) OF WAVE1 COMPLEX, SUBUNIT.

Entry informationi

Entry nameiWASF1_HUMAN
AccessioniPrimary (citable) accession number: Q92558
Secondary accession number(s): E1P5F2, Q5SZK7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: September 3, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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