Reviewed, UniProtKB/Swiss-Prot Q92556 (ELMO1_HUMAN)

Names and origin

Protein names

Recommended name:
    Engulfment and cell motility protein 1
Alternative name(s):
    CED-12 homolog

Gene names

Name:	ELMO1
Synonyms:	KIAA0281

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	727 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Acts in assocation with DOCK1 and CRK. Was initially proposed to be required in complex with DOCK1 to activate Rac Rho small GTPases. May enhance the guanine nucleotide exchange factor (GEF) activity of DOCK1.
Subunit structure	Interacts directly with the SH3-domain of DOCK1 via its SH3-binding site. Part of a complex with DOCK1 and RAC1. Part of a complex with DOCK1 and CRK isoform CRK-II. Interacts with PLEKHG6.
Subcellular location	Cytoplasm. Cell membrane. Note= Translocation to plasma membrane seems to be mediated by DOCK1 and CRK.
Tissue specificity	Widely expressed, with a higher expression in the spleen and placenta.
Post-translational modification	Phosphorylated by HCK Probable.
Sequence similarities	Contains 1 ELMO domain. Contains 1 PH domain.

Ontologies

Keywords
Biological process	Apoptosis Phagocytosis
Cellular component	Cell membrane Cytoplasm Membrane
Coding sequence diversity	Alternative splicing
Domain	SH3-binding
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	Rac protein signal transduction Ref.1 Inferred from genetic interaction. Source: UniProtKB actin cytoskeleton organization and biogenesis Ref.1 Inferred from genetic interaction. Source: UniProtKB apoptosis Ref.1 Non-traceable author statement. Source: UniProtKB cell motion Ref.1 Inferred from genetic interaction. Source: UniProtKB phagocytosis, engulfment Ref.1 Inferred from genetic interaction. Source: UniProtKB
Cellular component	cytosol Inferred from Experiment. Source: Reactome plasma membrane Ref.1 Inferred from direct assay. Source: UniProtKB
Molecular function	SH3 domain binding Ref.5 Inferred from physical interaction. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
DOCK1	Q14185	2	EBI-346417,EBI-446740
HCK	P08631	4	EBI-346417,EBI-346340
RHOG	P84095	2	EBI-346417,EBI-446579

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q92556-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q92556-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-480: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 727	727	Engulfment and cell motility protein 1
Regions
	Domain	319 – 492	174	ELMO
	Domain	555 – 676	122	PH
	Motif	707 – 714	8	SH3-binding
Natural variations
	Alternative sequence	1 – 480	480	Missing in isoform 2.
Experimental info
	Sequence conflict	716	1	E → A in CAB66721. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 16, 2003. Version 2. Checksum: 053DCA73D2B0A4C6	FASTA	727	83,829
	10 20 30 40 50 60 MPPPADIVKV AIEWPGAYPK LMEIDQKKPL SAIIKEVCDG WSLANHEYFA LQHADSSNFY 70 80 90 100 110 120 ITEKNRNEIK NGTILRLTTS PAQNAQQLHE RIQSSSMDAK LEALKDLASL SRDVTFAQEF 130 140 150 160 170 180 INLDGISLLT QMVESGTERY QKLQKIMKPC FGDMLSFTLT AFVELMDHGI VSWDTFSVAF 190 200 210 220 230 240 IKKIASFVNK SAIDISILQR SLAILESMVL NSHDLYQKVA QEITIGQLIP HLQGSDQEIQ 250 260 270 280 290 300 TYTIAVINAL FLKAPDERRQ EMANILAQKQ LRSIILTHVI RAQRAINNEM AHQLYVLQVL 310 320 330 340 350 360 TFNLLEDRMM TKMDPQDQAQ RDIIFELRRI AFDAESEPNN SSGSMEKRKS MYTRDYKKLG 370 380 390 400 410 420 FINHVNPAMD FTQTPPGMLA LDNMLYFAKH HQDAYIRIVL ENSSREDKHE CPFGRSSIEL 430 440 450 460 470 480 TKMLCEILKV GELPSETCND FHPMFFTHDR SFEEFFCICI QLLNKTWKEM RATSEDFNKV 490 500 510 520 530 540 MQVVKEQVMR ALTTKPSSLD QFKSKLQNLS YTEILKIRQS ERMNQEDFQS RPILELKEKI 550 560 570 580 590 600 QPEILELIKQ QRLNRLVEGT CFRKLNARRR QDKFWYCRLS PNHKVLHYGD LEESPQGEVP 610 620 630 640 650 660 HDSLQDKLPV ADIKAVVTGK DCPHMKEKGA LKQNKEVLEL AFSILYDSNC QLNFIAPDKH 670 680 690 700 710 720 EYCIWTDGLN ALLGKDMMSD LTRNDLDTLL SMEIKLRLLD LENIQIPDAP PPIPKEPSNY DFVYDCN
	Isoform 2 [UniParc]. Checksum: D02BCEC8D8A4383F		247	28,742
	10 20 30 40 50 60 MQVVKEQVMR ALTTKPSSLD QFKSKLQNLS YTEILKIRQS ERMNQEDFQS RPILELKEKI 70 80 90 100 110 120 QPEILELIKQ QRLNRLVEGT CFRKLNARRR QDKFWYCRLS PNHKVLHYGD LEESPQGEVP 130 140 150 160 170 180 HDSLQDKLPV ADIKAVVTGK DCPHMKEKGA LKQNKEVLEL AFSILYDSNC QLNFIAPDKH 190 200 210 220 230 240 EYCIWTDGLN ALLGKDMMSD LTRNDLDTLL SMEIKLRLLD LENIQIPDAP PPIPKEPSNY DFVYDCN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"CED-12/ELMO, a novel member of the CrkII/Dock180/Rac pathway, is required for phagocytosis and cell migration." Gumienny T.L., Brugnera E., Tosello-Trampont A.-C., Kinchen J.M., Haney L.B., Nishiwaki K., Walk S.F., Nemergut M.E., Macara I.G., Francis R., Schedl T., Qin Y., Van Aelst L., Hengartner M.O., Ravichandran K.S. Cell 107:27-41(2001) [PubMed: 11595183] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, INTERACTION WITH DOCK1, SUBUNIT OF A COMPLEX WITH DOCK1 AND CRK.
[2]	"Construction and characterization of human brain cDNA libraries suitable for analysis of cDNA clones encoding relatively large proteins." Ohara O., Nagase T., Ishikawa K., Nakajima D., Ohira M., Seki N., Nomura N. DNA Res. 4:53-59(1997) [PubMed: 9179496] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Brain.
[3]	"Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs." Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S., Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W., Ottenwaelder B., Obermaier B. , Tampe J., Heubner D., Wambutt R., Korn B., Klein M., Poustka A. Genome Res. 11:422-435(2001) [PubMed: 11230166] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Testis.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Brain, Lung and Pancreas.
[5]	"Identification of novel SH3 domain ligands for the Src family kinase Hck. Wiskott-Aldrich syndrome protein (WASP), WASP-interacting protein (WIP), and ELMO1." Scott M.P., Zappacosta F., Kim E.Y., Annan R.S., Miller W.T. J. Biol. Chem. 277:28238-28246(2002) [PubMed: 12029088] [Abstract] Cited for: PHOSPHORYLATION.
[6]	"Unconventional Rac-GEF activity is mediated through the Dock180-ELMO complex." Brugnera E., Haney L., Grimsley C., Lu M., Walk S.F., Tosello-Trampont A.-C., Macara I.G., Madhani H., Fink G.R., Ravichandran K.S. Nat. Cell Biol. 4:574-582(2002) [PubMed: 12134158] [Abstract] Cited for: FUNCTION, INTERACTION WITH DOCK1, SUBUNIT OF A COMPLEX CONTAINING RAC1 AND DOCK1.
[7]	"Interaction of ezrin with the novel guanine nucleotide exchange factor PLEKHG6 promotes RhoG-dependent apical cytoskeleton rearrangements in epithelial cells." D'Angelo R., Aresta S., Blangy A., Del Maestro L., Louvard D., Arpin M. Mol. Biol. Cell 18:4780-4793(2007) [PubMed: 17881735] [Abstract] Cited for: INTERACTION WITH PLEKHG6.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF398885 mRNA. Translation: AAL14466.1. D87457 mRNA. Translation: BAA13397.2. Different initiation. AL136787 mRNA. Translation: CAB66721.1. BC003051 mRNA. Translation: AAH03051.1. BC064635 mRNA. Translation: AAH64635.1. BC077074 mRNA. Translation: AAH77074.1. BC114341 mRNA. Translation: AAI14342.1.
RefSeq	NP_001034548.1. NP_055615.8. NP_569709.1.
UniGene	Hs.656638
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q92556.
PTM databases
PhosphoSite	Q92556.
Proteomic databases
PeptideAtlas	Q92556.
Genome annotation databases
Ensembl	ENSG00000155849. Homo sapiens. [Contig view]
GeneID	9844.
KEGG	hsa:9844.
Organism-specific databases
H-InvDB	HIX0020858.
HGNC	HGNC:16286. ELMO1.
MIM	606420. gene.
PharmGKB	PA27754.
HUGE	Search...
GenAtlas	Search...
GeneCards	Search...
Phylogenomic databases
HOGENOM	Q92556.
HOVERGEN	Q92556.
Enzyme and pathway databases
Reactome	REACT_6185. HIV Infection.
Gene expression databases
ArrayExpress	Q92556.
CleanEx	HS_ELMO1.
GermOnline	ENSG00000155849. Homo sapiens.
Family and domain databases
InterPro	IPR006816. ELM. IPR001849. PH. IPR011993. PH_type. [Graphical view]
Gene3D	G3DSA:2.30.29.30. PH_type. 1 hit.
Pfam	PF04727. ELMO_CED12. 1 hit. [Graphical view]
SMART	SM00233. PH. 1 hit. [Graphical view]
PROSITE	PS51335. ELMO. 1 hit. PS50003. PH_DOMAIN. False negative. [Graphical view]
ProDom	Q92556. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
SOURCE	Search...
ProtoNet	Search...

Entry information

Entry name

ELMO1_HUMAN

Accession

Primary (citable) accession number: Q92556
Secondary accession number(s): Q29R79, Q96PB0, Q9H0I1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	May 16, 2003
Last modified:	July 22, 2008
This is version 81 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)