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Protein

Proteasome inhibitor PI31 subunit

Gene

PSMF1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays an important role in control of proteasome function. Inhibits the hydrolysis of protein and peptide substrates by the 20S proteasome. Also inhibits the activation of the proteasome by the proteasome regulatory proteins PA700 and PA28.1 Publication

GO - Molecular functioni

  • endopeptidase inhibitor activity Source: UniProtKB
  • proteasome binding Source: UniProtKB
  • protein heterodimerization activity Source: ParkinsonsUK-UCL
  • protein homodimerization activity Source: ParkinsonsUK-UCL

GO - Biological processi

Enzyme and pathway databases

ReactomeiR-HSA-1169091. Activation of NF-kappaB in B cells.
R-HSA-1234176. Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha.
R-HSA-1236974. ER-Phagosome pathway.
R-HSA-1236978. Cross-presentation of soluble exogenous antigens (endosomes).
R-HSA-174084. Autodegradation of Cdh1 by Cdh1:APC/C.
R-HSA-174113. SCF-beta-TrCP mediated degradation of Emi1.
R-HSA-174154. APC/C:Cdc20 mediated degradation of Securin.
R-HSA-174178. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
R-HSA-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-HSA-180534. Vpu mediated degradation of CD4.
R-HSA-180585. Vif-mediated degradation of APOBEC3G.
R-HSA-187577. SCF(Skp2)-mediated degradation of p27/p21.
R-HSA-195253. Degradation of beta-catenin by the destruction complex.
R-HSA-202424. Downstream TCR signaling.
R-HSA-211733. Regulation of activated PAK-2p34 by proteasome mediated degradation.
R-HSA-2467813. Separation of Sister Chromatids.
R-HSA-2871837. FCERI mediated NF-kB activation.
R-HSA-349425. Autodegradation of the E3 ubiquitin ligase COP1.
R-HSA-350562. Regulation of ornithine decarboxylase (ODC).
R-HSA-382556. ABC-family proteins mediated transport.
R-HSA-446652. Interleukin-1 family signaling.
R-HSA-450408. AUF1 (hnRNP D0) binds and destabilizes mRNA.
R-HSA-4608870. Asymmetric localization of PCP proteins.
R-HSA-4641257. Degradation of AXIN.
R-HSA-4641258. Degradation of DVL.
R-HSA-5358346. Hedgehog ligand biogenesis.
R-HSA-5362768. Hh mutants that don't undergo autocatalytic processing are degraded by ERAD.
R-HSA-5607761. Dectin-1 mediated noncanonical NF-kB signaling.
R-HSA-5607764. CLEC7A (Dectin-1) signaling.
R-HSA-5610780. Degradation of GLI1 by the proteasome.
R-HSA-5610783. Degradation of GLI2 by the proteasome.
R-HSA-5610785. GLI3 is processed to GLI3R by the proteasome.
R-HSA-5632684. Hedgehog 'on' state.
R-HSA-5658442. Regulation of RAS by GAPs.
R-HSA-5668541. TNFR2 non-canonical NF-kB pathway.
R-HSA-5676590. NIK-->noncanonical NF-kB signaling.
R-HSA-5678895. Defective CFTR causes cystic fibrosis.
R-HSA-5687128. MAPK6/MAPK4 signaling.
R-HSA-5689603. UCH proteinases.
R-HSA-5689880. Ub-specific processing proteases.
R-HSA-68827. CDT1 association with the CDC6:ORC:origin complex.
R-HSA-68949. Orc1 removal from chromatin.
R-HSA-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-HSA-69229. Ubiquitin-dependent degradation of Cyclin D1.
R-HSA-69481. G2/M Checkpoints.
R-HSA-69601. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
R-HSA-8852276. The role of GTSE1 in G2/M progression after G2 checkpoint.
R-HSA-8854050. FBXL7 down-regulates AURKA during mitotic entry and in early mitosis.
R-HSA-8939236. RUNX1 regulates transcription of genes involved in differentiation of HSCs.
R-HSA-8941858. Regulation of RUNX3 expression and activity.
R-HSA-8948751. Regulation of PTEN stability and activity.
R-HSA-8951664. Neddylation.
R-HSA-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome inhibitor PI31 subunit
Short name:
hPI31
Gene namesi
Name:PSMF1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 20

Organism-specific databases

EuPathDBiHostDB:ENSG00000125818.17.
HGNCiHGNC:9571. PSMF1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Proteasome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi6V → R: Abolishes homodimerization. 1 Publication1
Mutagenesisi83I → E: Abolishes interaction with FBXO7, but has no effect on homodimerization; when associated with E-90. 1 Publication1
Mutagenesisi90I → E: Abolishes interaction with FBXO7, but has no effect on homodimerization; when associated with E-83. 1 Publication1

Organism-specific databases

OpenTargetsiENSG00000125818.
PharmGKBiPA33917.

Polymorphism and mutation databases

BioMutaiPSMF1.
DMDMi134047876.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00002209202 – 271Proteasome inhibitor PI31 subunitAdd BLAST270

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Modified residuei153PhosphoserineCombined sources1
Modified residuei205Omega-N-methylarginineBy similarity1
Modified residuei219Asymmetric dimethylarginineBy similarity1
Modified residuei231Omega-N-methylarginineCombined sources1
Modified residuei252PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

EPDiQ92530.
MaxQBiQ92530.
PaxDbiQ92530.
PeptideAtlasiQ92530.
PRIDEiQ92530.

PTM databases

iPTMnetiQ92530.
PhosphoSitePlusiQ92530.
SwissPalmiQ92530.

Expressioni

Gene expression databases

BgeeiENSG00000125818.
CleanExiHS_PSMF1.
ExpressionAtlasiQ92530. baseline and differential.
GenevisibleiQ92530. HS.

Organism-specific databases

HPAiHPA041122.
HPA041300.

Interactioni

Subunit structurei

Monomer and homodimer. Interacts with FBXO7. Interacts with the 20S proteasome.2 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • proteasome binding Source: UniProtKB
  • protein heterodimerization activity Source: ParkinsonsUK-UCL
  • protein homodimerization activity Source: ParkinsonsUK-UCL

Protein-protein interaction databases

BioGridi114872. 54 interactors.
IntActiQ92530. 31 interactors.
MINTiMINT-1633308.
STRINGi9606.ENSP00000327704.

Structurei

Secondary structure

1271
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 11Combined sources8
Helixi12 – 14Combined sources3
Helixi18 – 31Combined sources14
Turni32 – 34Combined sources3
Beta strandi35 – 43Combined sources9
Beta strandi51 – 53Combined sources3
Turni56 – 59Combined sources4
Beta strandi62 – 73Combined sources12
Beta strandi77 – 85Combined sources9
Beta strandi88 – 94Combined sources7
Turni96 – 99Combined sources4
Beta strandi101 – 107Combined sources7
Helixi108 – 111Combined sources4
Turni115 – 118Combined sources4
Helixi120 – 123Combined sources4
Beta strandi124 – 126Combined sources3
Helixi127 – 137Combined sources11
Helixi139 – 149Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2VT8X-ray2.60A/B1-151[»]
4OUHX-ray2.00A/B/C/D1-158[»]
ProteinModelPortaliQ92530.
SMRiQ92530.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ92530.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 150Important for homodimerization and interaction with FBXO71 PublicationAdd BLAST149

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi154 – 271Pro-richAdd BLAST118

Sequence similaritiesi

Belongs to the proteasome inhibitor PI31 family.Curated

Phylogenomic databases

eggNOGiKOG4761. Eukaryota.
ENOG4111GK4. LUCA.
GeneTreeiENSGT00390000012257.
HOGENOMiHOG000231957.
HOVERGENiHBG053746.
InParanoidiQ92530.
KOiK06700.
OMAiRPFGFPD.
OrthoDBiEOG091G0JIA.
PhylomeDBiQ92530.
TreeFamiTF106238.

Family and domain databases

InterProiView protein in InterPro
IPR013886. PI31_Prot_C.
IPR021625. PI31_Prot_N.
PfamiView protein in Pfam
PF08577. PI31_Prot_C. 1 hit.
PF11566. PI31_Prot_N. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q92530-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGLEVLFAS AAPAITCRQD ALVCFLHWEV VTHGYFGLGV GDQPGPNDKK
60 70 80 90 100
SELLPAGWNN NKDLYVLRYE YKDGSRKLLV KAITVESSMI LNVLEYGSQQ
110 120 130 140 150
VADLTLNLDD YIDAEHLGDF HRTYKNSEEL RSRIVSGIIT PIHEQWEKAN
160 170 180 190 200
VSSPHREFPP ATAREVDPLR IPPHHPHTSR QPPWCDPLGP FVVGGEDLDP
210 220 230 240 250
FGPRRGGMIV DPLRSGFPRA LIDPSSGLPN RLPPGAVPPG ARFDPFGPIG
260 270
TSPPGPNPDH LPPPGYDDMY L
Length:271
Mass (Da):29,817
Last modified:March 20, 2007 - v2
Checksum:iC669F8210AFAD727
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_02456436F → C3 PublicationsCorresponds to variant dbSNP:rs1803415Ensembl.1
Natural variantiVAR_022153174H → R. Corresponds to variant dbSNP:rs2235587Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D88378 mRNA. Translation: BAA13603.1.
AL031665 Genomic DNA. Translation: CAC10383.1.
CH471133 Genomic DNA. Translation: EAX10650.1.
CH471133 Genomic DNA. Translation: EAX10651.1.
BC126462 mRNA. Translation: AAI26463.1.
CCDSiCCDS13010.1.
RefSeqiNP_001310336.1. NM_001323407.1.
NP_006805.2. NM_006814.4.
NP_848693.2. NM_178578.3.
UniGeneiHs.471917.
Hs.656042.

Genome annotation databases

EnsembliENST00000333082; ENSP00000327704; ENSG00000125818.
ENST00000335877; ENSP00000338039; ENSG00000125818.
GeneIDi9491.
KEGGihsa:9491.
UCSCiuc002wel.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiPSMF1_HUMAN
AccessioniPrimary (citable) accession number: Q92530
Secondary accession number(s): A0AVQ9, D3DVW3, Q9H4I1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: March 20, 2007
Last modified: October 25, 2017
This is version 157 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 20
    Human chromosome 20: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. SIMILARITY comments
    Index of protein domains and families