Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

GPI mannosyltransferase 3

Gene

PIGB

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Mannosyltransferase involved in glycosylphosphatidylinositol-anchor biosynthesis. Transfers the third alpha-1,2-mannose to Man2-GlcN-acyl-PI during GPI precursor assembly.1 Publication

Pathwayi

GO - Molecular functioni

  1. mannosyltransferase activity Source: UniProtKB

GO - Biological processi

  1. cellular protein metabolic process Source: Reactome
  2. C-terminal protein lipidation Source: Reactome
  3. GPI anchor biosynthetic process Source: UniProtKB
  4. mannosylation Source: GOC
  5. post-translational protein modification Source: Reactome
  6. preassembly of GPI anchor in ER membrane Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

GPI-anchor biosynthesis

Enzyme and pathway databases

ReactomeiREACT_952. Synthesis of glycosylphosphatidylinositol (GPI).
UniPathwayiUPA00196.

Protein family/group databases

CAZyiGT22. Glycosyltransferase Family 22.

Names & Taxonomyi

Protein namesi
Recommended name:
GPI mannosyltransferase 3 (EC:2.4.1.-)
Alternative name(s):
GPI mannosyltransferase III
Short name:
GPI-MT-III
Phosphatidylinositol-glycan biosynthesis class B protein
Short name:
PIG-B
Gene namesi
Name:PIGB
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 15

Organism-specific databases

HGNCiHGNC:8959. PIGB.

Subcellular locationi

Endoplasmic reticulum membrane 1 Publication; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei63 – 8321HelicalSequence AnalysisAdd
BLAST
Transmembranei136 – 15621HelicalSequence AnalysisAdd
BLAST
Transmembranei192 – 21221HelicalSequence AnalysisAdd
BLAST
Transmembranei224 – 24421HelicalSequence AnalysisAdd
BLAST
Transmembranei255 – 27521HelicalSequence AnalysisAdd
BLAST
Transmembranei315 – 33521HelicalSequence AnalysisAdd
BLAST
Transmembranei340 – 36021HelicalSequence AnalysisAdd
BLAST
Transmembranei362 – 38221HelicalSequence AnalysisAdd
BLAST
Transmembranei387 – 40721HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: UniProtKB
  2. integral component of membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA33290.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 554554GPI mannosyltransferase 3PRO_0000246251Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi26 – 261N-linked (GlcNAc...)Sequence Analysis
Glycosylationi427 – 4271N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ92521.
PaxDbiQ92521.
PRIDEiQ92521.

PTM databases

PhosphoSiteiQ92521.

Expressioni

Gene expression databases

BgeeiQ92521.
CleanExiHS_PIGB.
ExpressionAtlasiQ92521. baseline and differential.
GenevestigatoriQ92521.

Organism-specific databases

HPAiHPA039929.

Interactioni

Protein-protein interaction databases

BioGridi114870. 2 interactions.
STRINGi9606.ENSP00000164305.

Structurei

3D structure databases

ProteinModelPortaliQ92521.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG276773.
GeneTreeiENSGT00550000075012.
HOGENOMiHOG000189846.
HOVERGENiHBG082136.
InParanoidiQ92521.
KOiK05286.
OMAiFLQCPPN.
OrthoDBiEOG761BV9.
PhylomeDBiQ92521.
TreeFamiTF313518.

Family and domain databases

InterProiIPR005599. GPI_mannosylTrfase.
[Graphical view]
PANTHERiPTHR22760. PTHR22760. 1 hit.
PfamiPF03901. Glyco_transf_22. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q92521-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRRPLSKCGM EPGGGDASLT LHGLQNRSHG KIKLRKRKST LYFNTQEKSA
60 70 80 90 100
RRRGDLLGEN IYLLLFTIAL RILNCFLVQT SFVPDEYWQS LEVSHHMVFN
110 120 130 140 150
YGYLTWEWTE RLRSYTYPLI FASIYKILHL LGKDSVQLLI WIPRLAQALL
160 170 180 190 200
SAVADVRLYS LMKQLENQEV ARWVFFCQLC SWFTWYCCTR TLTNTMETVL
210 220 230 240 250
TIIALFYYPL EGSKSMNSVK YSSLVALAFI IRPTAVILWT PLLFRHFCQE
260 270 280 290 300
PRKLDLILHH FLPVGFVTLS LSLMIDRIFF GQWTLVQFNF LKFNVLQNWG
310 320 330 340 350
TFYGSHPWHW YFSQGFPVIL GTHLPFFIHG CYLAPKRYRI LLVTVLWTLL
360 370 380 390 400
VYSMLSHKEF RFIYPVLPFC MVFCGYSLTH LKTWKKPALS FLFLSNLFLA
410 420 430 440 450
LYTGLVHQRG TLDVMSHIQK VCYNNPNKSS ASIFIMMPCH STPYYSHVHC
460 470 480 490 500
PLPMRFLQCP PDLTGKSHYL DEADVFYLNP LNWLHREFHD DASLPTHLIT
510 520 530 540 550
FSILEEEISA FLISSNYKRT AVFFHTHLPE GRIGSHIYVY ERKLKGKFNM

KMKF
Length:554
Mass (Da):65,056
Last modified:February 1, 1997 - v1
Checksum:iB2AF87D13ADF90B3
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti198 – 1981T → I in BAD97050 (Ref. 2) Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti68 – 681I → L.1 Publication
Corresponds to variant rs17851556 [ dbSNP | Ensembl ].
VAR_027027
Natural varianti162 – 1621M → T.
Corresponds to variant rs2290344 [ dbSNP | Ensembl ].
VAR_027028
Natural varianti299 – 2991W → L.2 Publications
Corresponds to variant rs678892 [ dbSNP | Ensembl ].
VAR_027029
Natural varianti484 – 4841L → S.1 Publication
Corresponds to variant rs17851554 [ dbSNP | Ensembl ].
VAR_027030
Natural varianti502 – 5021S → G.
Corresponds to variant rs652397 [ dbSNP | Ensembl ].
VAR_049224
Natural varianti551 – 5511K → T.
Corresponds to variant rs2444042 [ dbSNP | Ensembl ].
VAR_027031

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D42138 mRNA. Translation: BAA07709.1.
AK223330 mRNA. Translation: BAD97050.1.
BC017711 mRNA. Translation: AAH17711.1.
CCDSiCCDS61641.1.
PIRiS71751.
RefSeqiNP_004846.4. NM_004855.4.
UniGeneiHs.612814.

Genome annotation databases

EnsembliENST00000164305; ENSP00000164305; ENSG00000069943.
GeneIDi9488.
KEGGihsa:9488.
UCSCiuc002act.3. human.

Polymorphism databases

DMDMi74716597.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D42138 mRNA. Translation: BAA07709.1.
AK223330 mRNA. Translation: BAD97050.1.
BC017711 mRNA. Translation: AAH17711.1.
CCDSiCCDS61641.1.
PIRiS71751.
RefSeqiNP_004846.4. NM_004855.4.
UniGeneiHs.612814.

3D structure databases

ProteinModelPortaliQ92521.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114870. 2 interactions.
STRINGi9606.ENSP00000164305.

Protein family/group databases

CAZyiGT22. Glycosyltransferase Family 22.

PTM databases

PhosphoSiteiQ92521.

Polymorphism databases

DMDMi74716597.

Proteomic databases

MaxQBiQ92521.
PaxDbiQ92521.
PRIDEiQ92521.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000164305; ENSP00000164305; ENSG00000069943.
GeneIDi9488.
KEGGihsa:9488.
UCSCiuc002act.3. human.

Organism-specific databases

CTDi9488.
GeneCardsiGC15P055611.
HGNCiHGNC:8959. PIGB.
HPAiHPA039929.
MIMi604122. gene.
neXtProtiNX_Q92521.
PharmGKBiPA33290.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG276773.
GeneTreeiENSGT00550000075012.
HOGENOMiHOG000189846.
HOVERGENiHBG082136.
InParanoidiQ92521.
KOiK05286.
OMAiFLQCPPN.
OrthoDBiEOG761BV9.
PhylomeDBiQ92521.
TreeFamiTF313518.

Enzyme and pathway databases

UniPathwayiUPA00196.
ReactomeiREACT_952. Synthesis of glycosylphosphatidylinositol (GPI).

Miscellaneous databases

ChiTaRSiPIGB. human.
GeneWikiiPIGB.
GenomeRNAii9488.
NextBioi35552.
PROiQ92521.
SOURCEiSearch...

Gene expression databases

BgeeiQ92521.
CleanExiHS_PIGB.
ExpressionAtlasiQ92521. baseline and differential.
GenevestigatoriQ92521.

Family and domain databases

InterProiIPR005599. GPI_mannosylTrfase.
[Graphical view]
PANTHERiPTHR22760. PTHR22760. 1 hit.
PfamiPF03901. Glyco_transf_22. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "PIG-B, a membrane protein of the endoplasmic reticulum with a large lumenal domain, is involved in transferring the third mannose of the GPI anchor."
    Takahashi M., Inoue N., Ohishi K., Maeda Y., Nakamura N., Endo Y., Fujita T., Takeda J., Kinoshita T.
    EMBO J. 15:4254-4261(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION.
  2. Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S.
    Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANTS LEU-68; LEU-299 AND SER-484.
    Tissue: Testis.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT LEU-299.
    Tissue: Prostate.
  4. "Glycoinositol phospholipid anchor-defective K562 mutants with biochemical lesions distinct from those in Thy-1- murine lymphoma mutants."
    Mohney R.P., Knez J.J., Ravi L., Sevlever D., Rosenberry T.L., Hirose S., Medof M.E.
    J. Biol. Chem. 269:6536-6542(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION.

Entry informationi

Entry nameiPIGB_HUMAN
AccessioniPrimary (citable) accession number: Q92521
Secondary accession number(s): Q53FF9, Q8WVN7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: February 1, 1997
Last modified: January 7, 2015
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 15
    Human chromosome 15: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.