Reviewed, UniProtKB/Swiss-Prot Q92521 (PIGB_HUMAN)

Names and origin

Protein names

Recommended name:
    GPI mannosyltransferase 3
    EC=2.4.1.-
Alternative name(s):
    GPI mannosyltransferase III
      Short name=GPI-MT-III
    Phosphatidylinositol-glycan biosynthesis class B protein
      Short name=PIG-B

Gene names

Name:

PIGB

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	554 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Mannosyltransferase involved in glycosylphosphatidylinositol-anchor biosynthesis. Transfers the third alpha-1,2-mannose to Man2-GlcN-acyl-PI during GPI precursor assembly. Ref.1
Pathway	Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein. Ref.1
Sequence similarities	Belongs to the glycosyltransferase 22 family. PIGB subfamily.

Ontologies

Keywords
Biological process	GPI-anchor biosynthesis
Cellular component	Endoplasmic reticulum Membrane
Coding sequence diversity	Polymorphism
Domain	Transmembrane
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	preassembly of GPI anchor in ER membrane Inferred from Experiment. Source: Reactome
Cellular component	integral to membrane Ref.1 Non-traceable author statement. Source: UniProtKB intrinsic to endoplasmic reticulum membrane Inferred from electronic annotation. Source: InterPro
Molecular function	glycolipid mannosyltransferase activity Ref.1 Inferred from Experiment. Source: Reactome
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 554	554	GPI mannosyltransferase 3		PRO_0000246251
Regions
	Transmembrane	63 – 83	21	Potential
	Transmembrane	136 – 156	21	Potential
	Transmembrane	192 – 212	21	Potential
	Transmembrane	224 – 244	21	Potential
	Transmembrane	255 – 275	21	Potential
	Transmembrane	315 – 335	21	Potential
	Transmembrane	340 – 360	21	Potential
	Transmembrane	362 – 382	21	Potential
	Transmembrane	387 – 407	21	Potential
Amino acid modifications
	Glycosylation	26	1	N-linked (GlcNAc...) Potential
	Glycosylation	427	1	N-linked (GlcNAc...) Potential
Natural variations
	Natural variant	68	1	I → L: dbSNP rs17851556. Ref.2		VAR_027027
	Natural variant	162	1	M → T: dbSNP rs2290344.		VAR_027028
	Natural variant	299	1	W → L: dbSNP rs678892. Ref.2 Ref.3		VAR_027029
	Natural variant	484	1	L → S: dbSNP rs17851554. Ref.2		VAR_027030
	Natural variant	502	1	S → G: dbSNP rs652397.		VAR_049224
	Natural variant	551	1	K → T: dbSNP rs2444042.		VAR_027031
Experimental info
	Sequence conflict	198	1	T → I in BAD97050. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q92521-1 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: B2AF87D13ADF90B3	FASTA	554	65,056
	10 20 30 40 50 60 MRRPLSKCGM EPGGGDASLT LHGLQNRSHG KIKLRKRKST LYFNTQEKSA RRRGDLLGEN 70 80 90 100 110 120 IYLLLFTIAL RILNCFLVQT SFVPDEYWQS LEVSHHMVFN YGYLTWEWTE RLRSYTYPLI 130 140 150 160 170 180 FASIYKILHL LGKDSVQLLI WIPRLAQALL SAVADVRLYS LMKQLENQEV ARWVFFCQLC 190 200 210 220 230 240 SWFTWYCCTR TLTNTMETVL TIIALFYYPL EGSKSMNSVK YSSLVALAFI IRPTAVILWT 250 260 270 280 290 300 PLLFRHFCQE PRKLDLILHH FLPVGFVTLS LSLMIDRIFF GQWTLVQFNF LKFNVLQNWG 310 320 330 340 350 360 TFYGSHPWHW YFSQGFPVIL GTHLPFFIHG CYLAPKRYRI LLVTVLWTLL VYSMLSHKEF 370 380 390 400 410 420 RFIYPVLPFC MVFCGYSLTH LKTWKKPALS FLFLSNLFLA LYTGLVHQRG TLDVMSHIQK 430 440 450 460 470 480 VCYNNPNKSS ASIFIMMPCH STPYYSHVHC PLPMRFLQCP PDLTGKSHYL DEADVFYLNP 490 500 510 520 530 540 LNWLHREFHD DASLPTHLIT FSILEEEISA FLISSNYKRT AVFFHTHLPE GRIGSHIYVY 550 ERKLKGKFNM KMKF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"PIG-B, a membrane protein of the endoplasmic reticulum with a large lumenal domain, is involved in transferring the third mannose of the GPI anchor." Takahashi M., Inoue N., Ohishi K., Maeda Y., Nakamura N., Endo Y., Fujita T., Takeda J., Kinoshita T. EMBO J. 15:4254-4261(1996) [PubMed: 8861954] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION.
[2]	Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S. Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANTS LEU-68; LEU-299 AND SER-484. Tissue: Testis.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT LEU-299. Tissue: Prostate.
[4]	"Glycoinositol phospholipid anchor-defective K562 mutants with biochemical lesions distinct from those in Thy-1- murine lymphoma mutants." Mohney R.P., Knez J.J., Ravi L., Sevlever D., Rosenberry T.L., Hirose S., Medof M.E. J. Biol. Chem. 269:6536-6542(1994) [PubMed: 7907094] [Abstract] Cited for: IDENTIFICATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D42138 mRNA. Translation: BAA07709.1. AK223330 mRNA. Translation: BAD97050.1. BC017711 mRNA. Translation: AAH17711.1.
IPI	IPI00145121.
PIR	S71751.
RefSeq	NP_004846.4.
UniGene	Hs.612814
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT22. Glycosyltransferase Family 22.
PTM databases
PhosphoSite	Q92521.
Proteomic databases
PRIDE	Q92521.
Genome annotation databases
Ensembl	ENSG00000069943. Homo sapiens. [Contig view]
GeneID	9488.
KEGG	hsa:9488.
Organism-specific databases
GeneCards	GC15P053398.
H-InvDB	HIX0012262.
HGNC	HGNC:8959. PIGB.
MIM	604122. gene.
PharmGKB	PA33290.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q92521.
HOVERGEN	Q92521.
Enzyme and pathway databases
Reactome	REACT_1069. Post-translational protein modification.
Gene expression databases
ArrayExpress	Q92521.
Bgee	Q92521.
CleanEx	HS_PIGB.
GermOnline	ENSG00000069943. Homo sapiens.
Family and domain databases
InterPro	IPR005599. Alg9_trans. [Graphical view]
PANTHER	PTHR22760. Alg9_trans. 1 hit.
Pfam	PF03901. Glyco_transf_22. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	35552.
SOURCE	Search...

Entry information

Entry name

PIGB_HUMAN

Accession

Primary (citable) accession number: Q92521
Secondary accession number(s): Q53FF9, Q8WVN7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	February 1, 1997
Last modified:	April 14, 2009
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)