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Q92521

- PIGB_HUMAN

UniProt

Q92521 - PIGB_HUMAN

Protein

GPI mannosyltransferase 3

Gene

PIGB

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 91 (01 Oct 2014)
      Sequence version 1 (01 Feb 1997)
      Previous versions | rss
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    Functioni

    Mannosyltransferase involved in glycosylphosphatidylinositol-anchor biosynthesis. Transfers the third alpha-1,2-mannose to Man2-GlcN-acyl-PI during GPI precursor assembly.1 Publication

    Pathwayi

    GO - Molecular functioni

    1. mannosyltransferase activity Source: UniProtKB

    GO - Biological processi

    1. cellular protein metabolic process Source: Reactome
    2. C-terminal protein lipidation Source: Reactome
    3. GPI anchor biosynthetic process Source: UniProtKB
    4. mannosylation Source: GOC
    5. post-translational protein modification Source: Reactome
    6. preassembly of GPI anchor in ER membrane Source: Reactome

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    GPI-anchor biosynthesis

    Enzyme and pathway databases

    ReactomeiREACT_952. Synthesis of glycosylphosphatidylinositol (GPI).
    UniPathwayiUPA00196.

    Protein family/group databases

    CAZyiGT22. Glycosyltransferase Family 22.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    GPI mannosyltransferase 3 (EC:2.4.1.-)
    Alternative name(s):
    GPI mannosyltransferase III
    Short name:
    GPI-MT-III
    Phosphatidylinositol-glycan biosynthesis class B protein
    Short name:
    PIG-B
    Gene namesi
    Name:PIGB
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 15

    Organism-specific databases

    HGNCiHGNC:8959. PIGB.

    Subcellular locationi

    Endoplasmic reticulum membrane 1 Publication; Multi-pass membrane protein 1 Publication

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB
    2. integral component of membrane Source: UniProtKB

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA33290.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 554554GPI mannosyltransferase 3PRO_0000246251Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi26 – 261N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi427 – 4271N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    MaxQBiQ92521.
    PaxDbiQ92521.
    PRIDEiQ92521.

    PTM databases

    PhosphoSiteiQ92521.

    Expressioni

    Gene expression databases

    ArrayExpressiQ92521.
    BgeeiQ92521.
    CleanExiHS_PIGB.
    GenevestigatoriQ92521.

    Organism-specific databases

    HPAiHPA039929.

    Interactioni

    Protein-protein interaction databases

    BioGridi114870. 1 interaction.
    STRINGi9606.ENSP00000164305.

    Structurei

    3D structure databases

    ProteinModelPortaliQ92521.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei63 – 8321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei136 – 15621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei192 – 21221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei224 – 24421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei255 – 27521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei315 – 33521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei340 – 36021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei362 – 38221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei387 – 40721HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG276773.
    HOGENOMiHOG000189846.
    HOVERGENiHBG082136.
    InParanoidiQ92521.
    KOiK05286.
    OMAiFLQCPPN.
    OrthoDBiEOG761BV9.
    PhylomeDBiQ92521.
    TreeFamiTF313518.

    Family and domain databases

    InterProiIPR005599. GPI_mannosylTrfase.
    [Graphical view]
    PANTHERiPTHR22760. PTHR22760. 1 hit.
    PfamiPF03901. Glyco_transf_22. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q92521-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRRPLSKCGM EPGGGDASLT LHGLQNRSHG KIKLRKRKST LYFNTQEKSA    50
    RRRGDLLGEN IYLLLFTIAL RILNCFLVQT SFVPDEYWQS LEVSHHMVFN 100
    YGYLTWEWTE RLRSYTYPLI FASIYKILHL LGKDSVQLLI WIPRLAQALL 150
    SAVADVRLYS LMKQLENQEV ARWVFFCQLC SWFTWYCCTR TLTNTMETVL 200
    TIIALFYYPL EGSKSMNSVK YSSLVALAFI IRPTAVILWT PLLFRHFCQE 250
    PRKLDLILHH FLPVGFVTLS LSLMIDRIFF GQWTLVQFNF LKFNVLQNWG 300
    TFYGSHPWHW YFSQGFPVIL GTHLPFFIHG CYLAPKRYRI LLVTVLWTLL 350
    VYSMLSHKEF RFIYPVLPFC MVFCGYSLTH LKTWKKPALS FLFLSNLFLA 400
    LYTGLVHQRG TLDVMSHIQK VCYNNPNKSS ASIFIMMPCH STPYYSHVHC 450
    PLPMRFLQCP PDLTGKSHYL DEADVFYLNP LNWLHREFHD DASLPTHLIT 500
    FSILEEEISA FLISSNYKRT AVFFHTHLPE GRIGSHIYVY ERKLKGKFNM 550
    KMKF 554
    Length:554
    Mass (Da):65,056
    Last modified:February 1, 1997 - v1
    Checksum:iB2AF87D13ADF90B3
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti198 – 1981T → I in BAD97050. 1 PublicationCurated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti68 – 681I → L.1 Publication
    Corresponds to variant rs17851556 [ dbSNP | Ensembl ].
    VAR_027027
    Natural varianti162 – 1621M → T.
    Corresponds to variant rs2290344 [ dbSNP | Ensembl ].
    VAR_027028
    Natural varianti299 – 2991W → L.2 Publications
    Corresponds to variant rs678892 [ dbSNP | Ensembl ].
    VAR_027029
    Natural varianti484 – 4841L → S.1 Publication
    Corresponds to variant rs17851554 [ dbSNP | Ensembl ].
    VAR_027030
    Natural varianti502 – 5021S → G.
    Corresponds to variant rs652397 [ dbSNP | Ensembl ].
    VAR_049224
    Natural varianti551 – 5511K → T.
    Corresponds to variant rs2444042 [ dbSNP | Ensembl ].
    VAR_027031

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D42138 mRNA. Translation: BAA07709.1.
    AK223330 mRNA. Translation: BAD97050.1.
    BC017711 mRNA. Translation: AAH17711.1.
    CCDSiCCDS61641.1.
    PIRiS71751.
    RefSeqiNP_004846.4. NM_004855.4.
    UniGeneiHs.612814.

    Genome annotation databases

    EnsembliENST00000164305; ENSP00000164305; ENSG00000069943.
    GeneIDi9488.
    KEGGihsa:9488.
    UCSCiuc002act.3. human.

    Polymorphism databases

    DMDMi74716597.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D42138 mRNA. Translation: BAA07709.1 .
    AK223330 mRNA. Translation: BAD97050.1 .
    BC017711 mRNA. Translation: AAH17711.1 .
    CCDSi CCDS61641.1.
    PIRi S71751.
    RefSeqi NP_004846.4. NM_004855.4.
    UniGenei Hs.612814.

    3D structure databases

    ProteinModelPortali Q92521.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 114870. 1 interaction.
    STRINGi 9606.ENSP00000164305.

    Protein family/group databases

    CAZyi GT22. Glycosyltransferase Family 22.

    PTM databases

    PhosphoSitei Q92521.

    Polymorphism databases

    DMDMi 74716597.

    Proteomic databases

    MaxQBi Q92521.
    PaxDbi Q92521.
    PRIDEi Q92521.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000164305 ; ENSP00000164305 ; ENSG00000069943 .
    GeneIDi 9488.
    KEGGi hsa:9488.
    UCSCi uc002act.3. human.

    Organism-specific databases

    CTDi 9488.
    GeneCardsi GC15P055611.
    HGNCi HGNC:8959. PIGB.
    HPAi HPA039929.
    MIMi 604122. gene.
    neXtProti NX_Q92521.
    PharmGKBi PA33290.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG276773.
    HOGENOMi HOG000189846.
    HOVERGENi HBG082136.
    InParanoidi Q92521.
    KOi K05286.
    OMAi FLQCPPN.
    OrthoDBi EOG761BV9.
    PhylomeDBi Q92521.
    TreeFami TF313518.

    Enzyme and pathway databases

    UniPathwayi UPA00196 .
    Reactomei REACT_952. Synthesis of glycosylphosphatidylinositol (GPI).

    Miscellaneous databases

    GeneWikii PIGB.
    GenomeRNAii 9488.
    NextBioi 35552.
    PROi Q92521.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q92521.
    Bgeei Q92521.
    CleanExi HS_PIGB.
    Genevestigatori Q92521.

    Family and domain databases

    InterProi IPR005599. GPI_mannosylTrfase.
    [Graphical view ]
    PANTHERi PTHR22760. PTHR22760. 1 hit.
    Pfami PF03901. Glyco_transf_22. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "PIG-B, a membrane protein of the endoplasmic reticulum with a large lumenal domain, is involved in transferring the third mannose of the GPI anchor."
      Takahashi M., Inoue N., Ohishi K., Maeda Y., Nakamura N., Endo Y., Fujita T., Takeda J., Kinoshita T.
      EMBO J. 15:4254-4261(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION.
    2. Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S.
      Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANTS LEU-68; LEU-299 AND SER-484.
      Tissue: Testis.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT LEU-299.
      Tissue: Prostate.
    4. "Glycoinositol phospholipid anchor-defective K562 mutants with biochemical lesions distinct from those in Thy-1- murine lymphoma mutants."
      Mohney R.P., Knez J.J., Ravi L., Sevlever D., Rosenberry T.L., Hirose S., Medof M.E.
      J. Biol. Chem. 269:6536-6542(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION.

    Entry informationi

    Entry nameiPIGB_HUMAN
    AccessioniPrimary (citable) accession number: Q92521
    Secondary accession number(s): Q53FF9, Q8WVN7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 25, 2006
    Last sequence update: February 1, 1997
    Last modified: October 1, 2014
    This is version 91 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 15
      Human chromosome 15: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3