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Q92521 (PIGB_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 89. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
GPI mannosyltransferase 3

EC=2.4.1.-
Alternative name(s):
GPI mannosyltransferase III
Short name=GPI-MT-III
Phosphatidylinositol-glycan biosynthesis class B protein
Short name=PIG-B
Gene names
Name:PIGB
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length554 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Mannosyltransferase involved in glycosylphosphatidylinositol-anchor biosynthesis. Transfers the third alpha-1,2-mannose to Man2-GlcN-acyl-PI during GPI precursor assembly. Ref.1

Pathway

Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein Ref.1.

Sequence similarities

Belongs to the glycosyltransferase 22 family. PIGB subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 554554GPI mannosyltransferase 3
PRO_0000246251

Regions

Transmembrane63 – 8321Helical; Potential
Transmembrane136 – 15621Helical; Potential
Transmembrane192 – 21221Helical; Potential
Transmembrane224 – 24421Helical; Potential
Transmembrane255 – 27521Helical; Potential
Transmembrane315 – 33521Helical; Potential
Transmembrane340 – 36021Helical; Potential
Transmembrane362 – 38221Helical; Potential
Transmembrane387 – 40721Helical; Potential

Amino acid modifications

Glycosylation261N-linked (GlcNAc...) Potential
Glycosylation4271N-linked (GlcNAc...) Potential

Natural variations

Natural variant681I → L. Ref.2
Corresponds to variant rs17851556 [ dbSNP | Ensembl ].
VAR_027027
Natural variant1621M → T.
Corresponds to variant rs2290344 [ dbSNP | Ensembl ].
VAR_027028
Natural variant2991W → L. Ref.2 Ref.3
Corresponds to variant rs678892 [ dbSNP | Ensembl ].
VAR_027029
Natural variant4841L → S. Ref.2
Corresponds to variant rs17851554 [ dbSNP | Ensembl ].
VAR_027030
Natural variant5021S → G.
Corresponds to variant rs652397 [ dbSNP | Ensembl ].
VAR_049224
Natural variant5511K → T.
Corresponds to variant rs2444042 [ dbSNP | Ensembl ].
VAR_027031

Experimental info

Sequence conflict1981T → I in BAD97050. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q92521 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: B2AF87D13ADF90B3

FASTA55465,056
        10         20         30         40         50         60 
MRRPLSKCGM EPGGGDASLT LHGLQNRSHG KIKLRKRKST LYFNTQEKSA RRRGDLLGEN 

        70         80         90        100        110        120 
IYLLLFTIAL RILNCFLVQT SFVPDEYWQS LEVSHHMVFN YGYLTWEWTE RLRSYTYPLI 

       130        140        150        160        170        180 
FASIYKILHL LGKDSVQLLI WIPRLAQALL SAVADVRLYS LMKQLENQEV ARWVFFCQLC 

       190        200        210        220        230        240 
SWFTWYCCTR TLTNTMETVL TIIALFYYPL EGSKSMNSVK YSSLVALAFI IRPTAVILWT 

       250        260        270        280        290        300 
PLLFRHFCQE PRKLDLILHH FLPVGFVTLS LSLMIDRIFF GQWTLVQFNF LKFNVLQNWG 

       310        320        330        340        350        360 
TFYGSHPWHW YFSQGFPVIL GTHLPFFIHG CYLAPKRYRI LLVTVLWTLL VYSMLSHKEF 

       370        380        390        400        410        420 
RFIYPVLPFC MVFCGYSLTH LKTWKKPALS FLFLSNLFLA LYTGLVHQRG TLDVMSHIQK 

       430        440        450        460        470        480 
VCYNNPNKSS ASIFIMMPCH STPYYSHVHC PLPMRFLQCP PDLTGKSHYL DEADVFYLNP 

       490        500        510        520        530        540 
LNWLHREFHD DASLPTHLIT FSILEEEISA FLISSNYKRT AVFFHTHLPE GRIGSHIYVY 

       550 
ERKLKGKFNM KMKF 

« Hide

References

« Hide 'large scale' references
[1]"PIG-B, a membrane protein of the endoplasmic reticulum with a large lumenal domain, is involved in transferring the third mannose of the GPI anchor."
Takahashi M., Inoue N., Ohishi K., Maeda Y., Nakamura N., Endo Y., Fujita T., Takeda J., Kinoshita T.
EMBO J. 15:4254-4261(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION.
[2]Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S.
Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANTS LEU-68; LEU-299 AND SER-484.
Tissue: Testis.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT LEU-299.
Tissue: Prostate.
[4]"Glycoinositol phospholipid anchor-defective K562 mutants with biochemical lesions distinct from those in Thy-1- murine lymphoma mutants."
Mohney R.P., Knez J.J., Ravi L., Sevlever D., Rosenberry T.L., Hirose S., Medof M.E.
J. Biol. Chem. 269:6536-6542(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D42138 mRNA. Translation: BAA07709.1.
AK223330 mRNA. Translation: BAD97050.1.
BC017711 mRNA. Translation: AAH17711.1.
CCDSCCDS61641.1.
PIRS71751.
RefSeqNP_004846.4. NM_004855.4.
UniGeneHs.612814.

3D structure databases

ProteinModelPortalQ92521.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid114870. 1 interaction.
STRING9606.ENSP00000164305.

Protein family/group databases

CAZyGT22. Glycosyltransferase Family 22.

PTM databases

PhosphoSiteQ92521.

Polymorphism databases

DMDM74716597.

Proteomic databases

MaxQBQ92521.
PaxDbQ92521.
PRIDEQ92521.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000164305; ENSP00000164305; ENSG00000069943.
GeneID9488.
KEGGhsa:9488.
UCSCuc002act.3. human.

Organism-specific databases

CTD9488.
GeneCardsGC15P055611.
HGNCHGNC:8959. PIGB.
HPAHPA039929.
MIM604122. gene.
neXtProtNX_Q92521.
PharmGKBPA33290.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG276773.
HOGENOMHOG000189846.
HOVERGENHBG082136.
InParanoidQ92521.
KOK05286.
OMAFLQCPPN.
OrthoDBEOG761BV9.
PhylomeDBQ92521.
TreeFamTF313518.

Enzyme and pathway databases

ReactomeREACT_17015. Metabolism of proteins.
UniPathwayUPA00196.

Gene expression databases

ArrayExpressQ92521.
BgeeQ92521.
CleanExHS_PIGB.
GenevestigatorQ92521.

Family and domain databases

InterProIPR005599. GPI_mannosylTrfase.
[Graphical view]
PANTHERPTHR22760. PTHR22760. 1 hit.
PfamPF03901. Glyco_transf_22. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiPIGB.
GenomeRNAi9488.
NextBio35552.
PROQ92521.
SOURCESearch...

Entry information

Entry namePIGB_HUMAN
AccessionPrimary (citable) accession number: Q92521
Secondary accession number(s): Q53FF9, Q8WVN7
Entry history
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: February 1, 1997
Last modified: July 9, 2014
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 15

Human chromosome 15: entries, gene names and cross-references to MIM