Q92521 (PIGB_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GPI mannosyltransferase 3
EC=2.4.1.-

Alternative name(s):
GPI mannosyltransferase III
Short name=GPI-MT-III
Phosphatidylinositol-glycan biosynthesis class B protein
Short name=PIG-B

Gene names

Name:

PIGB

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	554 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Mannosyltransferase involved in glycosylphosphatidylinositol-anchor biosynthesis. Transfers the third alpha-1,2-mannose to Man2-GlcN-acyl-PI during GPI precursor assembly. Ref.1
Pathway	Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein Ref.1.
Sequence similarities	Belongs to the glycosyltransferase 22 family. PIGB subfamily.

Ontologies

Keywords
Biological process	GPI-anchor biosynthesis
Cellular component	Endoplasmic reticulum Membrane
Coding sequence diversity	Polymorphism
Domain	Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	C-terminal protein lipidation Traceable author statement. Source: Reactome preassembly of GPI anchor in ER membrane Traceable author statement. Source: Reactome
Cellular_component	endoplasmic reticulum membrane Inferred from direct assay Ref.1. Source: UniProtKB integral to membrane Non-traceable author statement Ref.1. Source: UniProtKB intrinsic to endoplasmic reticulum membrane Inferred from electronic annotation. Source: InterPro
Molecular_function	mannosyltransferase activity Inferred from direct assay Ref.1. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 554	554	GPI mannosyltransferase 3		PRO_0000246251
Regions
	Transmembrane	63 – 83	21	Helical; Potential
	Transmembrane	136 – 156	21	Helical; Potential
	Transmembrane	192 – 212	21	Helical; Potential
	Transmembrane	224 – 244	21	Helical; Potential
	Transmembrane	255 – 275	21	Helical; Potential
	Transmembrane	315 – 335	21	Helical; Potential
	Transmembrane	340 – 360	21	Helical; Potential
	Transmembrane	362 – 382	21	Helical; Potential
	Transmembrane	387 – 407	21	Helical; Potential
Amino acid modifications
	Modified residue	39	1	Phosphoserine By similarity
	Glycosylation	26	1	N-linked (GlcNAc...) Potential
	Glycosylation	427	1	N-linked (GlcNAc...) Potential
Natural variations
	Natural variant	68	1	I → L. Ref.2 Corresponds to variant rs17851556 [ dbSNP | Ensembl ].		VAR_027027
	Natural variant	162	1	M → T. Corresponds to variant rs2290344 [ dbSNP | Ensembl ].		VAR_027028
	Natural variant	299	1	W → L. Ref.2 Ref.3 Corresponds to variant rs678892 [ dbSNP | Ensembl ].		VAR_027029
	Natural variant	484	1	L → S. Ref.2 Corresponds to variant rs17851554 [ dbSNP | Ensembl ].		VAR_027030
	Natural variant	502	1	S → G. Corresponds to variant rs652397 [ dbSNP | Ensembl ].		VAR_049224
	Natural variant	551	1	K → T. Corresponds to variant rs2444042 [ dbSNP | Ensembl ].		VAR_027031
Experimental info
	Sequence conflict	198	1	T → I in BAD97050. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q92521 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: B2AF87D13ADF90B3	FASTA	554	65,056
	10 20 30 40 50 60 MRRPLSKCGM EPGGGDASLT LHGLQNRSHG KIKLRKRKST LYFNTQEKSA RRRGDLLGEN 70 80 90 100 110 120 IYLLLFTIAL RILNCFLVQT SFVPDEYWQS LEVSHHMVFN YGYLTWEWTE RLRSYTYPLI 130 140 150 160 170 180 FASIYKILHL LGKDSVQLLI WIPRLAQALL SAVADVRLYS LMKQLENQEV ARWVFFCQLC 190 200 210 220 230 240 SWFTWYCCTR TLTNTMETVL TIIALFYYPL EGSKSMNSVK YSSLVALAFI IRPTAVILWT 250 260 270 280 290 300 PLLFRHFCQE PRKLDLILHH FLPVGFVTLS LSLMIDRIFF GQWTLVQFNF LKFNVLQNWG 310 320 330 340 350 360 TFYGSHPWHW YFSQGFPVIL GTHLPFFIHG CYLAPKRYRI LLVTVLWTLL VYSMLSHKEF 370 380 390 400 410 420 RFIYPVLPFC MVFCGYSLTH LKTWKKPALS FLFLSNLFLA LYTGLVHQRG TLDVMSHIQK 430 440 450 460 470 480 VCYNNPNKSS ASIFIMMPCH STPYYSHVHC PLPMRFLQCP PDLTGKSHYL DEADVFYLNP 490 500 510 520 530 540 LNWLHREFHD DASLPTHLIT FSILEEEISA FLISSNYKRT AVFFHTHLPE GRIGSHIYVY 550 ERKLKGKFNM KMKF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"PIG-B, a membrane protein of the endoplasmic reticulum with a large lumenal domain, is involved in transferring the third mannose of the GPI anchor." Takahashi M., Inoue N., Ohishi K., Maeda Y., Nakamura N., Endo Y., Fujita T., Takeda J., Kinoshita T. EMBO J. 15:4254-4261(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION.
[2]	Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S. Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANTS LEU-68; LEU-299 AND SER-484. Tissue: Testis.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT LEU-299. Tissue: Prostate.
[4]	"Glycoinositol phospholipid anchor-defective K562 mutants with biochemical lesions distinct from those in Thy-1- murine lymphoma mutants." Mohney R.P., Knez J.J., Ravi L., Sevlever D., Rosenberry T.L., Hirose S., Medof M.E. J. Biol. Chem. 269:6536-6542(1994) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D42138 mRNA. Translation: BAA07709.1. AK223330 mRNA. Translation: BAD97050.1. BC017711 mRNA. Translation: AAH17711.1.
IPI	IPI00145121.
PIR	S71751.
RefSeq	NP_004846.4. NM_004855.4.
UniGene	Hs.612814.
3D structure databases
ProteinModelPortal	Q92521.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000164305.
Protein family/group databases
CAZy	GT22. Glycosyltransferase Family 22.
PTM databases
PhosphoSite	Q92521.
Polymorphism databases
DMDM	74716597.
Proteomic databases
PaxDb	Q92521.
PRIDE	Q92521.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000164305; ENSP00000164305; ENSG00000069943.
GeneID	9488.
KEGG	hsa:9488.
UCSC	uc002act.3. human.
Organism-specific databases
CTD	9488.
GeneCards	GC15P055611.
HGNC	HGNC:8959. PIGB.
HPA	HPA039929.
MIM	604122. gene.
neXtProt	NX_Q92521.
PharmGKB	PA33290.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG276773.
HOGENOM	HOG000189846.
HOVERGEN	HBG082136.
InParanoid	Q92521.
KO	K05286.
OMA	TWEWTER.
OrthoDB	EOG4894MC.
Enzyme and pathway databases
Reactome	REACT_17015. Metabolism of proteins.
UniPathway	UPA00196.
Gene expression databases
ArrayExpress	Q92521.
Bgee	Q92521.
CleanEx	HS_PIGB.
Genevestigator	Q92521.
GermOnline	ENSG00000069943. Homo sapiens.
Family and domain databases
InterPro	IPR005599. GPI_mannosylTrfase. [Graphical view]
PANTHER	PTHR22760. PTHR22760. 1 hit.
Pfam	PF03901. Glyco_transf_22. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	9488.
NextBio	35552.
SOURCE	Search...

Entry information

Entry name

PIGB_HUMAN

Accession

Primary (citable) accession number: Q92521
Secondary accession number(s): Q53FF9, Q8WVN7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	February 1, 1997
Last modified:	April 3, 2013
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.