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Protein

Translocating chain-associated membrane protein 2

Gene

Tram2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Necessary for collagen type I synthesis. May couple the activity of the ER Ca2+ pump SERCA2B with the activity of the translocon. This coupling may increase the local Ca2+ concentration at the site of collagen synthesis, and a high Ca2+ concentration may be necessary for the function of molecular chaperones involved in collagen folding (By similarity).By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Translocation, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Translocating chain-associated membrane protein 2
Gene namesi
Name:Tram2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1924817. Tram2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei23 – 4321HelicalSequence analysisAdd
BLAST
Transmembranei76 – 9621HelicalSequence analysisAdd
BLAST
Transmembranei120 – 14021HelicalSequence analysisAdd
BLAST
Transmembranei160 – 18021HelicalSequence analysisAdd
BLAST
Transmembranei199 – 21921HelicalSequence analysisAdd
BLAST
Transmembranei251 – 27121HelicalSequence analysisAdd
BLAST
Transmembranei288 – 30821HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 370370Translocating chain-associated membrane protein 2PRO_0000185533Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi55 – 551N-linked (GlcNAc...)Sequence analysis
Glycosylationi115 – 1151N-linked (GlcNAc...)Sequence analysis
Glycosylationi358 – 3581N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ924Z5.
PaxDbiQ924Z5.
PRIDEiQ924Z5.

PTM databases

iPTMnetiQ924Z5.
PhosphoSiteiQ924Z5.

Expressioni

Gene expression databases

BgeeiQ924Z5.
GenevisibleiQ924Z5. MM.

Interactioni

Subunit structurei

Interacts with COL1A1 (By similarity). Interacts with SERCA2B.By similarity1 Publication

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000047992.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini112 – 321210TLCPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the TRAM family.Curated
Contains 1 TLC (TRAM/LAG1/CLN8) domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1608. Eukaryota.
ENOG410XSB3. LUCA.
GeneTreeiENSGT00510000046470.
HOGENOMiHOG000007494.
HOVERGENiHBG054402.
InParanoidiQ924Z5.
OMAiLFNAWAA.
OrthoDBiEOG7ZPNKC.
PhylomeDBiQ924Z5.
TreeFamiTF314319.

Family and domain databases

InterProiIPR006634. TLC-dom.
IPR013599. TRAM1.
IPR016447. Translocation_assoc_membrane.
[Graphical view]
PfamiPF08390. TRAM1. 1 hit.
PF03798. TRAM_LAG1_CLN8. 1 hit.
[Graphical view]
PIRSFiPIRSF005449. Translocation_assoc_membrane. 1 hit.
SMARTiSM00724. TLC. 1 hit.
[Graphical view]
PROSITEiPS50922. TLC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q924Z5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAFRRRTKSY PLFSQEFIIH NHADIGFCLV LCVLIGLMFE VTAKTAFLFI
60 70 80 90 100
LPQYNISVPT ADSETVHYHY GPKDLVTILF YVVITIIFHA VVQEYILDKI
110 120 130 140 150
SKRLHLSKVK HSKFNESGQL LVFHLSAVAW CFYVIVTEGY LTNPRSLWED
160 170 180 190 200
YPHVYLSFQV KFFYLGQLAY WLHSLPELYF QKVRKEEVPR QLQYICLYLL
210 220 230 240 250
HITGAYLLNL SRLGLILLLL QYSTEALFHM ARLFHFADEN NERLFNAWAA
260 270 280 290 300
VFGVTRLFIL TLAVLTIGFG LARVENQVFD PEKGNFNTLP CRLGMLLLVC
310 320 330 340 350
VAQAWLMWRF IHSQLRHWRE YWKEQSAKRR VSAVPRPPAK LLKREPGYHE
360 370
NGVVKAENGT SSRTKKLKSP
Length:370
Mass (Da):43,183
Last modified:December 1, 2001 - v1
Checksum:iF8E768AFB0582548
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY029530 mRNA. Translation: AAK40298.1.
AK082751 mRNA. Translation: BAC38601.1.
BC018212 mRNA. Translation: AAH18212.1.
CCDSiCCDS14846.1.
RefSeqiNP_803128.1. NM_177409.3.
UniGeneiMm.185680.
Mm.273241.

Genome annotation databases

EnsembliENSMUST00000037998; ENSMUSP00000047992; ENSMUSG00000041779.
GeneIDi170829.
KEGGimmu:170829.
UCSCiuc007alg.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY029530 mRNA. Translation: AAK40298.1.
AK082751 mRNA. Translation: BAC38601.1.
BC018212 mRNA. Translation: AAH18212.1.
CCDSiCCDS14846.1.
RefSeqiNP_803128.1. NM_177409.3.
UniGeneiMm.185680.
Mm.273241.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000047992.

PTM databases

iPTMnetiQ924Z5.
PhosphoSiteiQ924Z5.

Proteomic databases

MaxQBiQ924Z5.
PaxDbiQ924Z5.
PRIDEiQ924Z5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000037998; ENSMUSP00000047992; ENSMUSG00000041779.
GeneIDi170829.
KEGGimmu:170829.
UCSCiuc007alg.2. mouse.

Organism-specific databases

CTDi9697.
MGIiMGI:1924817. Tram2.

Phylogenomic databases

eggNOGiKOG1608. Eukaryota.
ENOG410XSB3. LUCA.
GeneTreeiENSGT00510000046470.
HOGENOMiHOG000007494.
HOVERGENiHBG054402.
InParanoidiQ924Z5.
OMAiLFNAWAA.
OrthoDBiEOG7ZPNKC.
PhylomeDBiQ924Z5.
TreeFamiTF314319.

Miscellaneous databases

PROiQ924Z5.
SOURCEiSearch...

Gene expression databases

BgeeiQ924Z5.
GenevisibleiQ924Z5. MM.

Family and domain databases

InterProiIPR006634. TLC-dom.
IPR013599. TRAM1.
IPR016447. Translocation_assoc_membrane.
[Graphical view]
PfamiPF08390. TRAM1. 1 hit.
PF03798. TRAM_LAG1_CLN8. 1 hit.
[Graphical view]
PIRSFiPIRSF005449. Translocation_assoc_membrane. 1 hit.
SMARTiSM00724. TLC. 1 hit.
[Graphical view]
PROSITEiPS50922. TLC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Hartmann E.
    Submitted (APR-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  4. "TRAM2 protein interacts with endoplasmic reticulum Ca2+ pump Serca2b and is necessary for collagen type I synthesis."
    Stefanovic B., Stefanovic L., Schnabl B., Bataller R., Brenner D.A.
    Mol. Cell. Biol. 24:1758-1768(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SERCA2B.

Entry informationi

Entry nameiTRAM2_MOUSE
AccessioniPrimary (citable) accession number: Q924Z5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: December 1, 2001
Last modified: June 8, 2016
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.