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Protein

Serine/threonine-protein kinase 33

Gene

Stk33

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Serine/threonine protein kinase which phosphorylates VIME. May play a specific role in the dynamic behavior of the intermediate filament cytoskeleton by phosphorylation of VIME.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei140 – 1401ATPPROSITE-ProRule annotation
Active sitei233 – 2331Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi117 – 1259ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • protein serine/threonine kinase activity Source: UniProtKB

GO - Biological processi

  • protein autophosphorylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.1. 3474.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase 33 (EC:2.7.11.1)
Gene namesi
Name:Stk33
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:2152419. Stk33.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 491491Serine/threonine-protein kinase 33PRO_0000278477Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei403 – 4031PhosphoserineCombined sources

Post-translational modificationi

Autophosphorylated.

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ924X7.
PaxDbiQ924X7.
PRIDEiQ924X7.

PTM databases

iPTMnetiQ924X7.
PhosphoSiteiQ924X7.

Expressioni

Tissue specificityi

Highly expressed in testis, particularly in cells from the spermatogenic epithelia. Significant expression is detected in lung epithelia, alveolar macrophages, horizontal cells in the retina and in embryonic organs such as heart, brain and spinal cord. Also expressed in pituitary gland, kidney, pancreas, trachea and thyroid gland.1 Publication

Gene expression databases

BgeeiQ924X7.
CleanExiMM_STK33.
ExpressionAtlasiQ924X7. baseline and differential.
GenevisibleiQ924X7. MM.

Interactioni

Subunit structurei

Interacts with VIME.1 Publication

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000087897.

Structurei

3D structure databases

ProteinModelPortaliQ924X7.
SMRiQ924X7. Positions 21-422.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini111 – 377267Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0032. Eukaryota.
ENOG410XRMJ. LUCA.
GeneTreeiENSGT00840000129744.
HOGENOMiHOG000293198.
HOVERGENiHBG094004.
InParanoidiQ924X7.
KOiK08813.
OMAiENETRWI.
OrthoDBiEOG712TWR.
PhylomeDBiQ924X7.
TreeFamiTF314166.

Family and domain databases

InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR24347. PTHR24347. 1 hit.
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q924X7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADPSLNDNP TACPHCASSQ AGLLCVCPAG KSPVLVVEMS QTSSIGSTEF
60 70 80 90 100
FASQERKKER NTSRESSLKD LSIRTSNVER KPQAQWSRSN VTVGKIPHIR
110 120 130 140 150
MDDGAGIEEF YTFGRILGQG SFGMVFEAID KETGAKWAIK KVNKEKAGSS
160 170 180 190 200
AMKLLEREVS ILKTVNHQHI IHLEQVFESP QKMYLVMELC EDGELKAVMD
210 220 230 240 250
QRGHFSENET RLIIQSLASA IAYLHNKDIV HRDLKLENIM VKSSFIDDNN
260 270 280 290 300
EMNLNIKVTD FGLSVQKHGS RSEGMMQTTC GTPIYMAPEV INAHDYSQQC
310 320 330 340 350
DIWSIGVIMF ILLCGEPPFL ANSEEKLYEL IKKGELRFEN PVWESVSDSA
360 370 380 390 400
KNTLKQLMKV DPAHRITAKE LLDNQWLTGN TLSSARPTNV LEMMKEWKNN
410 420 430 440 450
PESDEETNTD EETEQSAVYS PSANTAKQPT NAAKKPAAES VGMTSSNSSS
460 470 480 490
SKLLSAESKA EPEKSSETVG HASVAKTTLK STTLFRGKKR L
Length:491
Mass (Da):54,459
Last modified:February 20, 2007 - v2
Checksum:i10F2490ACC4394B8
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti92 – 921Missing in AK014819 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK014819 mRNA. No translation available.
AJ307671 Genomic DNA. Translation: CAC39171.1.
AM056057 mRNA. Translation: CAJ20841.1.
CCDSiCCDS52358.1.
RefSeqiNP_473444.1. NM_054103.1.
XP_006507287.1. XM_006507224.1.
XP_006507288.1. XM_006507225.2.
XP_006507289.1. XM_006507226.2.
XP_011239951.1. XM_011241649.1.
XP_011239952.1. XM_011241650.1.
XP_011239953.1. XM_011241651.1.
XP_011239954.1. XM_011241652.1.
UniGeneiMm.389950.

Genome annotation databases

EnsembliENSMUST00000090414; ENSMUSP00000087897; ENSMUSG00000031027.
ENSMUST00000106745; ENSMUSP00000102356; ENSMUSG00000031027.
ENSMUST00000121748; ENSMUSP00000112515; ENSMUSG00000031027.
GeneIDi117229.
KEGGimmu:117229.
UCSCiuc009jdm.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK014819 mRNA. No translation available.
AJ307671 Genomic DNA. Translation: CAC39171.1.
AM056057 mRNA. Translation: CAJ20841.1.
CCDSiCCDS52358.1.
RefSeqiNP_473444.1. NM_054103.1.
XP_006507287.1. XM_006507224.1.
XP_006507288.1. XM_006507225.2.
XP_006507289.1. XM_006507226.2.
XP_011239951.1. XM_011241649.1.
XP_011239952.1. XM_011241650.1.
XP_011239953.1. XM_011241651.1.
XP_011239954.1. XM_011241652.1.
UniGeneiMm.389950.

3D structure databases

ProteinModelPortaliQ924X7.
SMRiQ924X7. Positions 21-422.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000087897.

PTM databases

iPTMnetiQ924X7.
PhosphoSiteiQ924X7.

Proteomic databases

MaxQBiQ924X7.
PaxDbiQ924X7.
PRIDEiQ924X7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000090414; ENSMUSP00000087897; ENSMUSG00000031027.
ENSMUST00000106745; ENSMUSP00000102356; ENSMUSG00000031027.
ENSMUST00000121748; ENSMUSP00000112515; ENSMUSG00000031027.
GeneIDi117229.
KEGGimmu:117229.
UCSCiuc009jdm.2. mouse.

Organism-specific databases

CTDi65975.
MGIiMGI:2152419. Stk33.

Phylogenomic databases

eggNOGiKOG0032. Eukaryota.
ENOG410XRMJ. LUCA.
GeneTreeiENSGT00840000129744.
HOGENOMiHOG000293198.
HOVERGENiHBG094004.
InParanoidiQ924X7.
KOiK08813.
OMAiENETRWI.
OrthoDBiEOG712TWR.
PhylomeDBiQ924X7.
TreeFamiTF314166.

Enzyme and pathway databases

BRENDAi2.7.11.1. 3474.

Miscellaneous databases

PROiQ924X7.
SOURCEiSearch...

Gene expression databases

BgeeiQ924X7.
CleanExiMM_STK33.
ExpressionAtlasiQ924X7. baseline and differential.
GenevisibleiQ924X7. MM.

Family and domain databases

InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR24347. PTHR24347. 1 hit.
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Testis.
  2. "Comparative genomic sequencing reveals a strikingly similar architecture of a conserved syntenic region on human chromosome 11p15.3 (including gene ST5) and mouse chromosome 7."
    Amid C., Bahr A., Mujica A., Sampson N., Bikar S.E., Winterpacht A., Zabel B., Hankeln T., Schmidt E.R.
    Cytogenet. Cell Genet. 93:284-290(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-378.
  3. "A novel serine/threonine kinase gene, STK33, on human chromosome 11p15.3."
    Mujica A.O., Hankeln T., Schmidt E.R.
    Gene 280:175-181(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 89-423.
    Strain: BALB/cJ.
  4. "Differential expression pattern of the novel serine/threonine kinase, STK33, in mice and men."
    Mujica A.O., Brauksiepe B., Saaler-Reinhardt S., Reuss S., Schmidt E.R.
    FEBS J. 272:4884-4898(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
  5. "The serine/threonine kinase Stk33 exhibits autophosphorylation and phosphorylates the intermediate filament protein Vimentin."
    Brauksiepe B., Mujica A.O., Herrmann H., Schmidt E.R.
    BMC Biochem. 9:25-25(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH VIME, AUTOPHOSPHORYLATION.
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-403, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Testis.

Entry informationi

Entry nameiSTK33_MOUSE
AccessioniPrimary (citable) accession number: Q924X7
Secondary accession number(s): Q4A1D4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: February 20, 2007
Last modified: June 8, 2016
This is version 98 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.