Q924W5 (SMC6_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Structural maintenance of chromosomes protein 6
Short name=SMC protein 6
Short name=SMC-6
Short name=mSMC6

Gene names

Name:	Smc6
Synonyms:	Kiaa4103, Smc6l1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	1097 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Core component of the SMC5-SMC6 complex, a complex involved in repair of DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination and mediates sumoylation of shelterin complex (telosome) components By similarity.
Subunit structure	Forms a heterodimer with SMC5. Component of the SMC5-SMC6 complex which consists at least of SMC5, SMC6, NSMCE2, NSMCE1, NSMCE4A or EID3 and NDNL2 By similarity.
Subcellular location	Nucleus. Chromosome. Chromosome › telomere By similarity. Note: Associates with chromatin. Ref.1
Domain	The flexible hinge domain, which separates the large intramolecular coiled coil regions, allows the heterotypic interaction with the corresponding domain of SMC5, forming a V-shaped heterodimer By similarity.
Post-translational modification	Phosphorylated By similarity. Sumoylated by NSMCE2/MMS21 By similarity. Ubiquitinated By similarity.
Sequence similarities	Belongs to the SMC family. SMC6 subfamily.
Sequence caution	The sequence BAB23051.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Biological process	DNA damage DNA recombination DNA repair
Cellular component	Chromosome Nucleus Telomere
Coding sequence diversity	Alternative splicing
Domain	Coiled coil
Ligand	ATP-binding Nucleotide-binding
PTM	Phosphoprotein Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular senescence Inferred from sequence or structural similarity. Source: UniProtKB double-strand break repair via homologous recombination Inferred from electronic annotation. Source: InterPro telomere maintenance via recombination Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	PML body Inferred from sequence or structural similarity. Source: UniProtKB Smc5-Smc6 complex Inferred from sequence or structural similarity. Source: UniProtKB chromosome, telomeric region Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q924W5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q924W5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     323-326: RLND → LQAS
     327-1097: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1097	1097	Structural maintenance of chromosomes protein 6		PRO_0000270957
Regions
	Nucleotide binding	82 – 89	8	ATP Potential
	Region	458 – 667	210	Flexible hinge
	Coiled coil	232 – 348	117	Potential
	Coiled coil	376 – 457	82	Potential
	Coiled coil	668 – 920	253	Potential
	Compositional bias	993 – 1028	36	Ala/Asp-rich (DA-box)
Amino acid modifications
	Modified residue	675	1	Phosphoserine By similarity
Natural variations
	Alternative sequence	323 – 326	4	RLND → LQAS in isoform 2.		VSP_022254
	Alternative sequence	327 – 1097	771	Missing in isoform 2.		VSP_022255
Experimental info
	Sequence conflict	120	1	Q → W in BAD90287. Ref.4
	Sequence conflict	351	1	E → K in BAE22709. Ref.2
	Sequence conflict	460	1	E → A in AAH99955. Ref.3
	Sequence conflict	628	1	A → V in AAH99955. Ref.3
	Sequence conflict	948	1	K → E in BAB29353. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 7329C25A85102DE5	FASTA	1,097	127,198
	10 20 30 40 50 60 MAKRKEENFC SPENAKRPRQ EELEDFDKDG DEDECTISFT NGTSTLTAAE VGIIESIQLR 70 80 90 100 110 120 NFMCHSMLGP FKFGSNVNFV VGNNGSGKSA VLTALIVGLG GKAVATNRGS SLKGFVKAGQ 130 140 150 160 170 180 NSADISITLR NRGDDAFRAN VYGDSIVVQQ HISVDGSRSY KLKSEKGTVV STRKEELIAI 190 200 210 220 230 240 LDHFNIQVDN PVSVLTQEMS KQFLQSKNEG DKYKFFMKAT QLEQMKEDYS YIMETKERTK 250 260 270 280 290 300 EQINQGEERL TELKRQCLEK EERFQNIAGL STMKTNLEYL KHEMAWAVVN EIEKQLNAIR 310 320 330 340 350 360 DNIKIGEERA AKLDRKMEEQ QVRLNDAEKK YKDIQDKLEK ISEETNARAP ECMALKTDVI 370 380 390 400 410 420 ARTRAFNDAE VLYNRSLNEY KALKKDGEQL CKRIEELKKS TDQSLEPERL ERQKRICWLK 430 440 450 460 470 480 EKVKALQDQE HTVNQEAEQF EQAIEKDKQE HGRVRKEDIE VRHALNYNQR QLKELKDSKT 490 500 510 520 530 540 DRLKRFGPHV PALLEAIDDA YRRRQFTHKP IGPLGACIHL RDPELALAIE SCLKGLLQAY 550 560 570 580 590 600 CCHNHADERV LQSLMKKFYP PGTSRPQIIV SEFRDEVYDV RLRAAYHPEF PTVLTALEID 610 620 630 640 650 660 NAVVANSLID MRSIETVLLI KNNSVARAVM QSQKPPKNCR EAFTADGDQV FAGRYYSSES 670 680 690 700 710 720 TRPKFLSRDV DSEISDLETE IENKKGHIIT LQQRLSALEK DIKRNEELLK RCQLHYKEIK 730 740 750 760 770 780 MKIRKNISEI RELENIEEHQ SVDIATLEDE AEENKIKMQM VEKNMEQQKE NMENLKSLKI 790 800 810 820 830 840 EAENKYDTIK LKINQLSELA DPLKDELNLA DSEVDSQKRG KQHYEDKQKE HLDTLNKKRR 850 860 870 880 890 900 ELDMKEKELQ EKMSQARQIC PERIEVKKSA SILDKEINRL RQKIQAEHAS HGDREEIMKQ 910 920 930 940 950 960 YQEARETYLD LDNKVRTLRR FIKLLEEIMT HRYKTYQQFR RCLTLRCKLY FDNLLSQRAY 970 980 990 1000 1010 1020 CGKMNFDHKN ETLSITVQPG EGNKASFNDM RALSGGERSF STVCFILSLW SIAESPFRCL 1030 1040 1050 1060 1070 1080 DEFDVYMDMV NRRIAMDMIL KMADSQRFRQ FILLTPQSMS SLPSSKLIRI LRMSDPERGQ 1090 TTLPFRPVTQ EEDDSAS
	Isoform 2 [UniParc]. Checksum: F4A36072831DA488	FASTA	326	36,744
	10 20 30 40 50 60 MAKRKEENFC SPENAKRPRQ EELEDFDKDG DEDECTISFT NGTSTLTAAE VGIIESIQLR 70 80 90 100 110 120 NFMCHSMLGP FKFGSNVNFV VGNNGSGKSA VLTALIVGLG GKAVATNRGS SLKGFVKAGQ 130 140 150 160 170 180 NSADISITLR NRGDDAFRAN VYGDSIVVQQ HISVDGSRSY KLKSEKGTVV STRKEELIAI 190 200 210 220 230 240 LDHFNIQVDN PVSVLTQEMS KQFLQSKNEG DKYKFFMKAT QLEQMKEDYS YIMETKERTK 250 260 270 280 290 300 EQINQGEERL TELKRQCLEK EERFQNIAGL STMKTNLEYL KHEMAWAVVN EIEKQLNAIR 310 320 DNIKIGEERA AKLDRKMEEQ QVLQAS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of a novel human SMC heterodimer homologous to the Schizosaccharomyces pombe Rad18/Spr18 complex." Taylor E.M., Moghraby J.S., Lees J.H., Smit B., Moens P.B., Lehmann A.R. Mol. Biol. Cell 12:1583-1594(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBCELLULAR LOCATION.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-767 AND 797-1097 (ISOFORM 1). Strain: C57BL/6J and NOD. Tissue: Egg, Extraembryonic tissue, Liver, Pancreas, Placenta, Thymus and Tongue.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: C57BL/6 and FVB/N. Tissue: Brain, Colon and Egg.
[4]	"Prediction of the coding sequences of mouse homologues of KIAA gene. The complete nucleotide sequences of mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries." Okazaki N., Kikuno R.F., Ohara R., Inamoto S., Nagase T., Ohara O., Koga H. Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 119-1097 (ISOFORM 1). Tissue: Fetal brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ310552 mRNA. Translation: CAC39250.1. AK003872 mRNA. Translation: BAB23051.1. Different initiation. AK009009 mRNA. Translation: BAB26022.3. AK011982 mRNA. Translation: BAB27956.1. AK012626 mRNA. Translation: BAB28365.1. AK014442 mRNA. Translation: BAB29353.2. AK050341 mRNA. Translation: BAC34200.2. AK088002 mRNA. Translation: BAC40087.2. AK135873 mRNA. Translation: BAE22709.1. AK148478 mRNA. Translation: BAE28576.1. BC026429 mRNA. Translation: AAH26429.1. BC048790 mRNA. Translation: AAH48790.1. BC090630 mRNA. Translation: AAH90630.1. BC099955 mRNA. Translation: AAH99955.1. AK220488 mRNA. Translation: BAD90287.1.
IPI	IPI00165850. IPI00816943.
RefSeq	NP_079971.2. NM_025695.4.
UniGene	Mm.173953.
3D structure databases
HSSP	HSSP built from PDB template 1US8 based on UniProtKB P58301.
ProteinModelPortal	Q924W5.
SMR	Q924W5. Positions 54-201, 991-1043.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q924W5. 9 interactions.
PTM databases
PhosphoSite	Q924W5.
Proteomic databases
PaxDb	Q924W5.
PRIDE	Q924W5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000020931; ENSMUSP00000020931; ENSMUSG00000020608.
GeneID	67241.
KEGG	mmu:67241.
UCSC	uc007nax.1. mouse. uc007nay.1. mouse.
Organism-specific databases
CTD	79677.
MGI	MGI:1914491. Smc6.
Rouge	Search...
Phylogenomic databases
eggNOG	COG1196.
GeneTree	ENSGT00550000074816.
HOVERGEN	HBG093964.
InParanoid	Q924W5.
OMA	LHYKELK.
OrthoDB	EOG44QT04.
Gene expression databases
Bgee	Q924W5.
CleanEx	MM_SMC6.
Genevestigator	Q924W5.
Family and domain databases
InterPro	IPR003395. RecF/RecN/SMC. IPR027132. SMC6. [Graphical view]
PANTHER	PTHR19306:SF2. PTHR19306:SF2. 1 hit.
Pfam	PF02463. SMC_N. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	323984.
SOURCE	Search...

Entry information

Entry name

SMC6_MOUSE

Accession

Primary (citable) accession number: Q924W5
Secondary accession number(s): Q3UFI5 , Q3UX54, Q499E1, Q5DTN2, Q8BFU9, Q8R0T4, Q9CSK7, Q9CV94, Q9CZZ5, Q9D169, Q9D6B2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 9, 2007
Last sequence update:	December 1, 2001
Last modified:	May 1, 2013
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program