Dual specificity testis-specific protein kinase 2

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Q924U5 (TESK2_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 96. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Dual specificity testis-specific protein kinase 2
EC=2.7.12.1

Alternative name(s):
Testicular protein kinase 2

Gene names

Name:

Tesk2

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	570 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Dual specificity protein kinase activity catalyzing autophosphorylation and phosphorylation of exogenous substrates on both serine/threonine and tyrosine residues. Phosphorylates cofilin at 'Ser-3'. May play an important role in spermatogenesis By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Cofactor	Magnesium By similarity. Manganese By similarity.
Enzyme regulation	Activated by autophosphorylation on Ser-219 By similarity.
Subcellular location	Nucleus By similarity.
Tissue specificity	Predominantly expressed in testis and prostate. Found predominantly in non-germinal Sertoli cells.
Sequence similarities	Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Contains 1 protein kinase domain.

Ontologies

Keywords
Cellular component	Nucleus
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase Tyrosine-protein kinase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	actin cytoskeleton organization Inferred from mutant phenotype Ref.1. Source: UniProtKB apoptotic process Non-traceable author statement Ref.1. Source: UniProtKB focal adhesion assembly Inferred from mutant phenotype Ref.1. Source: UniProtKB intracellular signal transduction Non-traceable author statement PubMed 10512679. Source: UniProtKB spermatogenesis Inferred from expression pattern PubMed 10512679. Source: UniProtKB
Cellular_component	cytoplasm Inferred from direct assay Ref.1. Source: RGD nucleus Inferred from direct assay Ref.1. Source: UniProtKB
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from direct assay Ref.1. Source: UniProtKB protein serine/threonine/tyrosine kinase activity Inferred from electronic annotation. Source: EC protein tyrosine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 570

570

Dual specificity testis-specific protein kinase 2

PRO_0000086751

Regions

Domain

58 – 313

256

Protein kinase

Nucleotide binding

64 – 72

ATP By similarity

Sites

Active site

176

Proton acceptor By similarity

Binding site

ATP By similarity

Amino acid modifications

Modified residue

219

Phosphoserine; by autocatalysis By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q924U5 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: D20073AD93EE9F9C
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FASTA

570

63,559

        10         20         30         40         50         60 
MDRSKRNSIA GFPPRVERLE EFEGGGGGDG NTVQVGRVSS SSYRAIISAF SRLTSLDDFT 

        70         80         90        100        110        120 
REKIGSGFFS EVFKVRHRAS GQVMALKMNT LSSNRANLLK EMQLMNRLSH PNILRFMGVC 

       130        140        150        160        170        180 
VHQGQLHALT EYINSGNLEQ LLDSNLYLPW TVRVKLAYDI AVGLSYLHFK GIFHRDLTSK 

       190        200        210        220        230        240 
NCLIKRDENG YSAVVADFGL AEKIPDASIG SEKLAVVGSP FWMAPEVLRD EPYNEKADVF 

       250        260        270        280        290        300 
SYGIILCEII ARIQADPDYL PRTENFGLDY DAFQHMVGDC PSDFLQLTFN CCNMDPKLRP 

       310        320        330        340        350        360 
SFEEIGKTLE EIMSRLQEEE LERDRKLQPT AKGLLEKVPG GKRLSSLDDK IPHKSPRPRR 

       370        380        390        400        410        420 
TIWLSRSQSD IFSRKPPRTV NVLDPYYQPR DGATHTPKVN PFSARQDLKG GKVKFFDLPS 

       430        440        450        460        470        480 
KSVISLVFDL DAPGPGTVSL ADCQEPLAPS SRRWRSLPGS PEFLHQACPF VGCEESLSDG 

       490        500        510        520        530        540 
PPPRLSSLKY RVREIPPFRT SALSATSAHE AMDCSNPQEE NGFVPRPKGT SPCSGAASEE 

       550        560        570 
MEVEEERPRR APVHFSISGI SLQTQGEQDG

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cofilin phosphorylation and actin reorganization activities of testicular protein kinase 2 and its predominant expression in testicular Sertoli cells." Toshima J., Toshima J.Y., Takeuchi K., Mori R., Mizuno K. J. Biol. Chem. 276:31449-31458(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB049402 mRNA. Translation: BAB62908.1. BC128727 mRNA. Translation: AAI28728.1.
IPI	IPI00205126.
RefSeq	NP_596887.1. NM_133396.2.
UniGene	Rn.144652.
3D structure databases
ProteinModelPortal	Q924U5.
ModBase	Search...
PTM databases
PhosphoSite	Q924U5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000023416; ENSRNOP00000023416; ENSRNOG00000017282.
GeneID	170908.
KEGG	rno:170908.
UCSC	RGD:619984. rat.
Organism-specific databases
CTD	10420.
RGD	619984. Tesk2.
Phylogenomic databases
eggNOG	COG0515.
GeneTree	ENSGT00530000063025.
HOGENOM	HOG000231415.
HOVERGEN	HBG058204.
InParanoid	Q924U5.
KO	K08842.
OMA	PQEENGF.
OrthoDB	EOG4NZTSW.
Enzyme and pathway databases
BRENDA	2.7.10.2. 5301.
Gene expression databases
ArrayExpress	Q924U5.
Genevestigator	Q924U5.
GermOnline	ENSRNOG00000017282. Rattus norvegicus.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR001245. Ser-Thr/Tyr_kinase_cat_dom. IPR008266. Tyr_kinase_AS. [Graphical view]
Pfam	PF07714. Pkinase_Tyr. 1 hit. [Graphical view]
PRINTS	PR00109. TYRKINASE.
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00109. PROTEIN_KINASE_TYR. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	621355.

Entry information

Entry name

TESK2_RAT

Accession

Primary (citable) accession number: Q924U5
Secondary accession number(s): A1A5M5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2002
Last sequence update:	December 1, 2001
Last modified:	April 3, 2013
This is version 96 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

PTM databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents