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Protein

Dual specificity testis-specific protein kinase 2

Gene

Tesk2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Dual specificity protein kinase activity catalyzing autophosphorylation and phosphorylation of exogenous substrates on both serine/threonine and tyrosine residues. Phosphorylates cofilin at 'Ser-3'. May play an important role in spermatogenesis (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Protein has several cofactor binding sites:

Enzyme regulationi

Activated by autophosphorylation on Ser-219.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei87 – 871ATPPROSITE-ProRule annotation
Active sitei176 – 1761Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi64 – 729ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. protein serine/threonine/tyrosine kinase activity Source: UniProtKB-EC
  4. protein serine/threonine kinase activity Source: UniProtKB
  5. protein tyrosine kinase activity Source: UniProtKB-KW

GO - Biological processi

  1. actin cytoskeleton organization Source: UniProtKB
  2. apoptotic process Source: UniProtKB
  3. focal adhesion assembly Source: UniProtKB
  4. intracellular signal transduction Source: UniProtKB
  5. protein phosphorylation Source: UniProtKB
  6. spermatogenesis Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase, Tyrosine-protein kinase

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.10.2. 5301.

Names & Taxonomyi

Protein namesi
Recommended name:
Dual specificity testis-specific protein kinase 2 (EC:2.7.12.1)
Alternative name(s):
Testicular protein kinase 2
Gene namesi
Name:Tesk2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 5

Organism-specific databases

RGDi619984. Tesk2.

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. cytoplasm Source: RGD
  2. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 570570Dual specificity testis-specific protein kinase 2PRO_0000086751Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei219 – 2191Phosphoserine; by autocatalysisBy similarity

Keywords - PTMi

Phosphoprotein

PTM databases

PhosphoSiteiQ924U5.

Expressioni

Tissue specificityi

Predominantly expressed in testis and prostate. Found predominantly in non-germinal Sertoli cells.

Gene expression databases

GenevestigatoriQ924U5.

Structurei

3D structure databases

ProteinModelPortaliQ924U5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini58 – 313256Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00530000063025.
HOGENOMiHOG000231415.
HOVERGENiHBG058204.
InParanoidiQ924U5.
KOiK08842.
OMAiNVLDPYY.
OrthoDBiEOG7V7664.
PhylomeDBiQ924U5.
TreeFamiTF318014.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008266. Tyr_kinase_AS.
[Graphical view]
PfamiPF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
PRINTSiPR00109. TYRKINASE.
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q924U5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDRSKRNSIA GFPPRVERLE EFEGGGGGDG NTVQVGRVSS SSYRAIISAF
60 70 80 90 100
SRLTSLDDFT REKIGSGFFS EVFKVRHRAS GQVMALKMNT LSSNRANLLK
110 120 130 140 150
EMQLMNRLSH PNILRFMGVC VHQGQLHALT EYINSGNLEQ LLDSNLYLPW
160 170 180 190 200
TVRVKLAYDI AVGLSYLHFK GIFHRDLTSK NCLIKRDENG YSAVVADFGL
210 220 230 240 250
AEKIPDASIG SEKLAVVGSP FWMAPEVLRD EPYNEKADVF SYGIILCEII
260 270 280 290 300
ARIQADPDYL PRTENFGLDY DAFQHMVGDC PSDFLQLTFN CCNMDPKLRP
310 320 330 340 350
SFEEIGKTLE EIMSRLQEEE LERDRKLQPT AKGLLEKVPG GKRLSSLDDK
360 370 380 390 400
IPHKSPRPRR TIWLSRSQSD IFSRKPPRTV NVLDPYYQPR DGATHTPKVN
410 420 430 440 450
PFSARQDLKG GKVKFFDLPS KSVISLVFDL DAPGPGTVSL ADCQEPLAPS
460 470 480 490 500
SRRWRSLPGS PEFLHQACPF VGCEESLSDG PPPRLSSLKY RVREIPPFRT
510 520 530 540 550
SALSATSAHE AMDCSNPQEE NGFVPRPKGT SPCSGAASEE MEVEEERPRR
560 570
APVHFSISGI SLQTQGEQDG
Length:570
Mass (Da):63,559
Last modified:December 1, 2001 - v1
Checksum:iD20073AD93EE9F9C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB049402 mRNA. Translation: BAB62908.1.
BC128727 mRNA. Translation: AAI28728.1.
RefSeqiNP_596887.1. NM_133396.2.
XP_006238690.1. XM_006238628.2.
UniGeneiRn.144652.

Genome annotation databases

EnsembliENSRNOT00000023416; ENSRNOP00000023416; ENSRNOG00000017282.
GeneIDi170908.
KEGGirno:170908.
UCSCiRGD:619984. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB049402 mRNA. Translation: BAB62908.1.
BC128727 mRNA. Translation: AAI28728.1.
RefSeqiNP_596887.1. NM_133396.2.
XP_006238690.1. XM_006238628.2.
UniGeneiRn.144652.

3D structure databases

ProteinModelPortaliQ924U5.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

PhosphoSiteiQ924U5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000023416; ENSRNOP00000023416; ENSRNOG00000017282.
GeneIDi170908.
KEGGirno:170908.
UCSCiRGD:619984. rat.

Organism-specific databases

CTDi10420.
RGDi619984. Tesk2.

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00530000063025.
HOGENOMiHOG000231415.
HOVERGENiHBG058204.
InParanoidiQ924U5.
KOiK08842.
OMAiNVLDPYY.
OrthoDBiEOG7V7664.
PhylomeDBiQ924U5.
TreeFamiTF318014.

Enzyme and pathway databases

BRENDAi2.7.10.2. 5301.

Miscellaneous databases

NextBioi621355.
PROiQ924U5.

Gene expression databases

GenevestigatoriQ924U5.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008266. Tyr_kinase_AS.
[Graphical view]
PfamiPF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
PRINTSiPR00109. TYRKINASE.
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cofilin phosphorylation and actin reorganization activities of testicular protein kinase 2 and its predominant expression in testicular Sertoli cells."
    Toshima J., Toshima J.Y., Takeuchi K., Mori R., Mizuno K.
    J. Biol. Chem. 276:31449-31458(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.

Entry informationi

Entry nameiTESK2_RAT
AccessioniPrimary (citable) accession number: Q924U5
Secondary accession number(s): A1A5M5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: December 1, 2001
Last modified: January 7, 2015
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.