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Q924U5

- TESK2_RAT

UniProt

Q924U5 - TESK2_RAT

Protein

Dual specificity testis-specific protein kinase 2

Gene

Tesk2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 106 (01 Oct 2014)
      Sequence version 1 (01 Dec 2001)
      Previous versions | rss
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    Functioni

    Dual specificity protein kinase activity catalyzing autophosphorylation and phosphorylation of exogenous substrates on both serine/threonine and tyrosine residues. Phosphorylates cofilin at 'Ser-3'. May play an important role in spermatogenesis By similarity.By similarity

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Cofactori

    Magnesium.By similarity
    Manganese.By similarity

    Enzyme regulationi

    Activated by autophosphorylation on Ser-219.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei87 – 871ATPPROSITE-ProRule annotation
    Active sitei176 – 1761Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi64 – 729ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. protein serine/threonine/tyrosine kinase activity Source: UniProtKB-EC
    4. protein serine/threonine kinase activity Source: UniProtKB
    5. protein tyrosine kinase activity Source: UniProtKB-KW

    GO - Biological processi

    1. actin cytoskeleton organization Source: UniProtKB
    2. apoptotic process Source: UniProtKB
    3. focal adhesion assembly Source: UniProtKB
    4. intracellular signal transduction Source: UniProtKB
    5. protein phosphorylation Source: UniProtKB
    6. spermatogenesis Source: UniProtKB

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase, Tyrosine-protein kinase

    Keywords - Ligandi

    ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi2.7.10.2. 5301.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Dual specificity testis-specific protein kinase 2 (EC:2.7.12.1)
    Alternative name(s):
    Testicular protein kinase 2
    Gene namesi
    Name:Tesk2
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 5

    Organism-specific databases

    RGDi619984. Tesk2.

    Subcellular locationi

    Nucleus By similarity

    GO - Cellular componenti

    1. cytoplasm Source: RGD
    2. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 570570Dual specificity testis-specific protein kinase 2PRO_0000086751Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei219 – 2191Phosphoserine; by autocatalysisBy similarity

    Keywords - PTMi

    Phosphoprotein

    PTM databases

    PhosphoSiteiQ924U5.

    Expressioni

    Tissue specificityi

    Predominantly expressed in testis and prostate. Found predominantly in non-germinal Sertoli cells.

    Gene expression databases

    GenevestigatoriQ924U5.

    Structurei

    3D structure databases

    ProteinModelPortaliQ924U5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini58 – 313256Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00530000063025.
    HOGENOMiHOG000231415.
    HOVERGENiHBG058204.
    InParanoidiQ924U5.
    KOiK08842.
    OMAiNVLDPYY.
    OrthoDBiEOG7V7664.
    PhylomeDBiQ924U5.
    TreeFamiTF318014.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
    IPR008266. Tyr_kinase_AS.
    [Graphical view]
    PfamiPF07714. Pkinase_Tyr. 1 hit.
    [Graphical view]
    PRINTSiPR00109. TYRKINASE.
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00109. PROTEIN_KINASE_TYR. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q924U5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDRSKRNSIA GFPPRVERLE EFEGGGGGDG NTVQVGRVSS SSYRAIISAF    50
    SRLTSLDDFT REKIGSGFFS EVFKVRHRAS GQVMALKMNT LSSNRANLLK 100
    EMQLMNRLSH PNILRFMGVC VHQGQLHALT EYINSGNLEQ LLDSNLYLPW 150
    TVRVKLAYDI AVGLSYLHFK GIFHRDLTSK NCLIKRDENG YSAVVADFGL 200
    AEKIPDASIG SEKLAVVGSP FWMAPEVLRD EPYNEKADVF SYGIILCEII 250
    ARIQADPDYL PRTENFGLDY DAFQHMVGDC PSDFLQLTFN CCNMDPKLRP 300
    SFEEIGKTLE EIMSRLQEEE LERDRKLQPT AKGLLEKVPG GKRLSSLDDK 350
    IPHKSPRPRR TIWLSRSQSD IFSRKPPRTV NVLDPYYQPR DGATHTPKVN 400
    PFSARQDLKG GKVKFFDLPS KSVISLVFDL DAPGPGTVSL ADCQEPLAPS 450
    SRRWRSLPGS PEFLHQACPF VGCEESLSDG PPPRLSSLKY RVREIPPFRT 500
    SALSATSAHE AMDCSNPQEE NGFVPRPKGT SPCSGAASEE MEVEEERPRR 550
    APVHFSISGI SLQTQGEQDG 570
    Length:570
    Mass (Da):63,559
    Last modified:December 1, 2001 - v1
    Checksum:iD20073AD93EE9F9C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB049402 mRNA. Translation: BAB62908.1.
    BC128727 mRNA. Translation: AAI28728.1.
    RefSeqiNP_596887.1. NM_133396.2.
    XP_006238690.1. XM_006238628.1.
    UniGeneiRn.144652.

    Genome annotation databases

    EnsembliENSRNOT00000023416; ENSRNOP00000023416; ENSRNOG00000017282.
    GeneIDi170908.
    KEGGirno:170908.
    UCSCiRGD:619984. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB049402 mRNA. Translation: BAB62908.1 .
    BC128727 mRNA. Translation: AAI28728.1 .
    RefSeqi NP_596887.1. NM_133396.2.
    XP_006238690.1. XM_006238628.1.
    UniGenei Rn.144652.

    3D structure databases

    ProteinModelPortali Q924U5.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei Q924U5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000023416 ; ENSRNOP00000023416 ; ENSRNOG00000017282 .
    GeneIDi 170908.
    KEGGi rno:170908.
    UCSCi RGD:619984. rat.

    Organism-specific databases

    CTDi 10420.
    RGDi 619984. Tesk2.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00530000063025.
    HOGENOMi HOG000231415.
    HOVERGENi HBG058204.
    InParanoidi Q924U5.
    KOi K08842.
    OMAi NVLDPYY.
    OrthoDBi EOG7V7664.
    PhylomeDBi Q924U5.
    TreeFami TF318014.

    Enzyme and pathway databases

    BRENDAi 2.7.10.2. 5301.

    Miscellaneous databases

    NextBioi 621355.
    PROi Q924U5.

    Gene expression databases

    Genevestigatori Q924U5.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
    IPR008266. Tyr_kinase_AS.
    [Graphical view ]
    Pfami PF07714. Pkinase_Tyr. 1 hit.
    [Graphical view ]
    PRINTSi PR00109. TYRKINASE.
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00109. PROTEIN_KINASE_TYR. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cofilin phosphorylation and actin reorganization activities of testicular protein kinase 2 and its predominant expression in testicular Sertoli cells."
      Toshima J., Toshima J.Y., Takeuchi K., Mori R., Mizuno K.
      J. Biol. Chem. 276:31449-31458(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Testis.

    Entry informationi

    Entry nameiTESK2_RAT
    AccessioniPrimary (citable) accession number: Q924U5
    Secondary accession number(s): A1A5M5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 8, 2002
    Last sequence update: December 1, 2001
    Last modified: October 1, 2014
    This is version 106 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3