Reviewed, UniProtKB/Swiss-Prot Q924U5 (TESK2_RAT)

Names and origin

Protein names

Recommended name:
    Dual specificity testis-specific protein kinase 2
    EC=2.7.12.1
Alternative name(s):
    Testicular protein kinase 2

Gene names

Name:

Tesk2

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	570 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Dual specificity protein kinase activity catalyzing autophosphorylation and phosphorylation of exogenous substrates on both serine/threonine and tyrosine residues. Phosphorylates cofilin at 'Ser-3'. May play an important role in spermatogenesis By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Cofactor	Magnesium By similarity. Manganese By similarity.
Enzyme regulation	Activated by autophosphorylation on Ser-219 By similarity.
Subcellular location	Nucleus By similarity.
Tissue specificity	Predominantly expressed in testis and prostate. Found predominantly in non-germinal Sertoli cells.
Sequence similarities	Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Contains 1 protein kinase domain.

Ontologies

Keywords
Cellular component	Nucleus
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase Tyrosine-protein kinase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	actin cytoskeleton organization Ref.1 Inferred from mutant phenotype. Source: UniProtKB apoptosis Ref.1 Non-traceable author statement. Source: UniProtKB focal adhesion formation Ref.1 Inferred from mutant phenotype. Source: UniProtKB intracellular signaling cascade Non-traceable author statement. Source: UniProtKB male meiosis Inferred from sequence or structural similarity. Source: UniProtKB protein amino acid phosphorylation Ref.1 Inferred from direct assay. Source: UniProtKB spermatogenesis Inferred from expression pattern. Source: UniProtKB
Cellular component	cytoplasm Ref.1 Inferred from mutant phenotype. Source: RGD nucleus Ref.1 Inferred from direct assay. Source: UniProtKB
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Ref.1 Inferred from direct assay. Source: UniProtKB protein tyrosine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 570	570	Dual specificity testis-specific protein kinase 2		PRO_0000086751
Regions
	Domain	58 – 313	256	Protein kinase
	Nucleotide binding	64 – 72	9	ATP By similarity
Sites
	Active site	176	1	Proton acceptor By similarity
	Binding site	87	1	ATP By similarity
Amino acid modifications
	Modified residue	219	1	Phosphoserine; by autocatalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q924U5-1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: D20073AD93EE9F9C	FASTA	570	63,559
	10 20 30 40 50 60 MDRSKRNSIA GFPPRVERLE EFEGGGGGDG NTVQVGRVSS SSYRAIISAF SRLTSLDDFT 70 80 90 100 110 120 REKIGSGFFS EVFKVRHRAS GQVMALKMNT LSSNRANLLK EMQLMNRLSH PNILRFMGVC 130 140 150 160 170 180 VHQGQLHALT EYINSGNLEQ LLDSNLYLPW TVRVKLAYDI AVGLSYLHFK GIFHRDLTSK 190 200 210 220 230 240 NCLIKRDENG YSAVVADFGL AEKIPDASIG SEKLAVVGSP FWMAPEVLRD EPYNEKADVF 250 260 270 280 290 300 SYGIILCEII ARIQADPDYL PRTENFGLDY DAFQHMVGDC PSDFLQLTFN CCNMDPKLRP 310 320 330 340 350 360 SFEEIGKTLE EIMSRLQEEE LERDRKLQPT AKGLLEKVPG GKRLSSLDDK IPHKSPRPRR 370 380 390 400 410 420 TIWLSRSQSD IFSRKPPRTV NVLDPYYQPR DGATHTPKVN PFSARQDLKG GKVKFFDLPS 430 440 450 460 470 480 KSVISLVFDL DAPGPGTVSL ADCQEPLAPS SRRWRSLPGS PEFLHQACPF VGCEESLSDG 490 500 510 520 530 540 PPPRLSSLKY RVREIPPFRT SALSATSAHE AMDCSNPQEE NGFVPRPKGT SPCSGAASEE 550 560 570 MEVEEERPRR APVHFSISGI SLQTQGEQDG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cofilin phosphorylation and actin reorganization activities of testicular protein kinase 2 and its predominant expression in testicular Sertoli cells." Toshima J., Toshima J.Y., Takeuchi K., Mori R., Mizuno K. J. Biol. Chem. 276:31449-31458(2001) [PubMed: 11418599] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB049402 mRNA. Translation: BAB62908.1. BC128727 mRNA. Translation: AAI28728.1.
IPI	IPI00205126.
RefSeq	NP_596887.1.
UniGene	Rn.144652
3D structure databases
HSSP	HSSP built from PDB template 1IAS based on UniProtKB P36897.
ModBase	Search...
PTM databases
PhosphoSite	Q924U5.
Genome annotation databases
Ensembl	ENSRNOT00000023416; ENSRNOP00000023416; ENSRNOG00000017282; Rattus norvegicus. [Genome view]
GeneID	170908.
KEGG	rno:170908.
UCSC	NM_133396. rat.
Organism-specific databases
CTD	170908.
RGD	619984. Tesk2.
Phylogenomic databases
HOVERGEN	Q924U5.
OMA	RRWRSLP.
Enzyme and pathway databases
BRENDA	2.7.10.2. 248. 2.7.12.1. 248.
Gene expression databases
ArrayExpress	Q924U5.
Genevestigator	Q924U5.
GermOnline	ENSRNOG00000017282. Rattus norvegicus.
Family and domain databases
InterPro	IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR001245. Tyr_pkinase. IPR008266. Tyr_pkinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
PRINTS	PR00109. TYRKINASE.
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00109. PROTEIN_KINASE_TYR. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	621355.

Entry information

Entry name

TESK2_RAT

Accession

Primary (citable) accession number: Q924U5
Secondary accession number(s): A1A5M5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2002
Last sequence update:	December 1, 2001
Last modified:	November 3, 2009
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)