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Protein

E3 ubiquitin-protein ligase RNF31

Gene

Rnf31

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

E3 ubiquitin-protein ligase component of the LUBAC complex which conjugates linear ('Met-1'-linked) polyubiquitin chains to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation. LUBAC is proposed to be recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex. Together with FAM105B/otulin, the LUBAC complex regulates the canonical Wnt signaling during angiogenesis. Binds polyubiquitin of different linkage types (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri293 – 32533RanBP2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri344 – 37330RanBP2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri403 – 43230RanBP2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri693 – 74149RING-type 1; degenerateAdd
BLAST
Zinc fingeri773 – 82654IBR-typeAdd
BLAST
Zinc fingeri854 – 90350RING-type 2Add
BLAST

GO - Molecular functioni

GO - Biological processi

  • CD40 signaling pathway Source: BHF-UCL
  • negative regulation of necroptotic process Source: MGI
  • positive regulation of I-kappaB kinase/NF-kappaB signaling Source: UniProtKB
  • positive regulation of NF-kappaB transcription factor activity Source: UniProtKB
  • positive regulation of protein targeting to mitochondrion Source: MGI
  • protein linear polyubiquitination Source: UniProtKB
  • protein polyubiquitination Source: UniProtKB
  • protein ubiquitination Source: BHF-UCL
  • T cell receptor signaling pathway Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-5357905. Regulation of TNFR1 signaling.
R-MMU-5357956. TNFR1-induced NFkappaB signaling pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase RNF31 (EC:6.3.2.-)
Alternative name(s):
HOIL-1-interacting protein
Short name:
HOIP
Putative Ariadne-like ubiquitin ligase
Short name:
PAUL
RING finger protein 31
Gene namesi
Name:Rnf31
Synonyms:Paul
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:1934704. Rnf31.

Subcellular locationi

GO - Cellular componenti

  • CD40 receptor complex Source: BHF-UCL
  • cytoplasmic side of plasma membrane Source: BHF-UCL
  • cytosol Source: MGI
  • LUBAC complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10661066E3 ubiquitin-protein ligase RNF31PRO_0000056070Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei377 – 3771PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ924T7.
MaxQBiQ924T7.
PaxDbiQ924T7.
PRIDEiQ924T7.

PTM databases

PhosphoSiteiQ924T7.

Expressioni

Tissue specificityi

Widely expressed (at protein level). Not expressed in heart.1 Publication

Gene expression databases

BgeeiQ924T7.
CleanExiMM_RNF31.
ExpressionAtlasiQ924T7. baseline and differential.
GenevisibleiQ924T7. MM.

Interactioni

Subunit structurei

Component of the LUBAC complex (linear ubiquitin chain assembly complex) which consists of SHARPIN, RBCK1 and RNF31. LUBAC has a MW of approximative 600 kDa suggesting a heteromultimeric assembly of its subunits. Associates with the TNF-R1 signaling complex (TNF-RSC) in a stimulation-dependent manner (By similarity). Interacts with MUSK. Interacts with FAM105B/otulin; the interaction is direct.By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
IkbkgO885227EBI-647680,EBI-998011

GO - Molecular functioni

Protein-protein interaction databases

BioGridi234548. 12 interactions.
DIPiDIP-49579N.
IntActiQ924T7. 4 interactions.
STRINGi10090.ENSMUSP00000019443.

Structurei

3D structure databases

ProteinModelPortaliQ924T7.
SMRiQ924T7. Positions 5-177, 345-373, 476-620, 684-1066.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini558 – 60952UBAPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 479479Polyubiquitin-bindingBy similarityAdd
BLAST
Regioni557 – 61054Interaction with RBCK1By similarityAdd
BLAST
Regioni904 – 1066163LDD domainBy similarityAdd
BLAST

Domaini

The RanBP2-type zinc fingers mediate the specific interaction with ubiquitin.By similarity
The UBA domain mediates association with RBCK1/HOIL1 via interaction with its UBL domain.By similarity
RING 1 and IBR zinc-fingers catalyze the first step transfer of ubiquitin from the E2 onto RING 2, to transiently form a HECT-like covalent thioester intermediate.By similarity
The linear ubiquitin chain determining domain (LDD) mediates the final transfer of ubiquitin from RING 2 onto the N-terminus of a target ubiquitin.By similarity

Sequence similaritiesi

Contains 1 IBR-type zinc finger.Curated
Contains 3 RanBP2-type zinc fingers.PROSITE-ProRule annotation
Contains 2 RING-type zinc fingers.Curated
Contains 1 UBA domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri293 – 32533RanBP2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri344 – 37330RanBP2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri403 – 43230RanBP2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri693 – 74149RING-type 1; degenerateAdd
BLAST
Zinc fingeri773 – 82654IBR-typeAdd
BLAST
Zinc fingeri854 – 90350RING-type 2Add
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1812. Eukaryota.
ENOG410XS1H. LUCA.
GeneTreeiENSGT00530000064112.
HOVERGENiHBG063678.
InParanoidiQ924T7.
KOiK11974.
OMAiRTNDPEY.
OrthoDBiEOG7BW0HN.
PhylomeDBiQ924T7.
TreeFamiTF350529.

Family and domain databases

InterProiIPR002867. IBR_dom.
IPR018997. PUB_domain.
IPR026254. RNF31.
IPR032065. RNF31-UBA.
IPR015940. UBA.
IPR001876. Znf_RanBP2.
IPR017907. Znf_RING_CS.
[Graphical view]
PANTHERiPTHR16004. PTHR16004. 1 hit.
PfamiPF16678. HOIP-UBA. 1 hit.
PF01485. IBR. 1 hit.
PF09409. PUB. 1 hit.
[Graphical view]
SMARTiSM00647. IBR. 2 hits.
SM00547. ZnF_RBZ. 3 hits.
[Graphical view]
SUPFAMiSSF143503. SSF143503. 1 hit.
SSF90209. SSF90209. 2 hits.
PROSITEiPS50030. UBA. 1 hit.
PS01358. ZF_RANBP2_1. 3 hits.
PS50199. ZF_RANBP2_2. 1 hit.
PS00518. ZF_RING_1. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q924T7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPGDEERGFL AAREELASAL RWDSAQVFPL EQLMPLLATS LPPAARYLQL
60 70 80 90 100
DAGRLVRCNA HGEPRNYLNT LSTALNILEK YGRNLLSPQR PRYWRSVKFN
110 120 130 140 150
NPVFRSTVDA VQGGRDVLRL YGYTEERPDG LSFPEGQEEP DEYQVAVVTL
160 170 180 190 200
EVLLLRTELS LLLQNTHPRQ NALDQLLRES VEDGMLQLSE FHPLLREIVP
210 220 230 240 250
GPRPSAQGST PGPCFLCGSA PGTLHCPACN QVSCPACDIL FHGHPSRAHH
260 270 280 290 300
LRQALPGSHQ TASLSSSLPA SSQPRPPSSS LALGDSSLSS PDPANACLPW
310 320 330 340 350
HCLTCATLNE PWAVFCAVCS QPKGCKVPGI EGSHGTGGLE PEPARDQWAC
360 370 380 390 400
QSCTFENEAA AVLCAICERP RLAQPPSLVV DSHDAGVCQQ SLKQEDPLLT
410 420 430 440 450
AAQPQVWYCD HCTFCNSGPV WVCAMCNRTR DPIPTQPALQ SYPSSLEKGR
460 470 480 490 500
PKPGSSQHLG SSLPASCGDP EKQRQDKMRK EGLQLVSMIQ EGETAGASPE
510 520 530 540 550
EVFSALQYSG TEVPLQWLRS ELSYVLEMVA ELAGQQDPEL GAFSCQEARK
560 570 580 590 600
AWLDRHGNLD EAVEECVRAR RRKVHELQSL GFGPKEGSLQ ALFQHGGDVA
610 620 630 640 650
RALTELQRQR LEPFHQRLWD RDPEPTPCWD GLDRQSLVRR LLAVYTLPSW
660 670 680 690 700
GRAELALALL QETPRNYELL DVVEAVRHSQ DRAFLRRLLA QECAVCGWAL
710 720 730 740 750
PRNRMQALIS CECTICPECF RQHFTIALKE KHITDMVCPA CGRPDLTDDA
760 770 780 790 800
QLLSYFSTLD IQLRESLDPD AYALFHKKLT EAVLMRDPKF LWCAQCSFGF
810 820 830 840 850
IYEREQLEAT CPQCHQTFCV RCKRQWEEQH RGRSCEDFQN WKRTNDPEYQ
860 870 880 890 900
AQGLAMYLQE NGIDCPKCKF SYALARGGCM HFHCTQCRHQ FCSGCYNAFY
910 920 930 940 950
AKNKCPDPNC KVKKSLHGHH PRDCLFYLRD WTAARLQKLL QDNNVMFNTE
960 970 980 990 1000
PPAGTRAVPG GGCRVMEQKE VHSGFRDEAC GKETPPGYAG LCQAHYKEYL
1010 1020 1030 1040 1050
VSLINAHSLD PATLYEVEEL ETATIRYLHL APQPADGEDL PAYQARLLQK
1060
LREEVPLGQS IARRRK
Length:1,066
Mass (Da):119,314
Last modified:March 15, 2004 - v2
Checksum:iC630572B8BDFC0AD
GO
Isoform 2 (identifier: Q924T7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     826-834: Missing.

Show »
Length:1,057
Mass (Da):118,148
Checksum:i4D25A3CC7BBA4415
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei826 – 8349Missing in isoform 2. CuratedVSP_009649

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB053120 Genomic DNA. Translation: BAB47406.1.
BC057595 mRNA. Translation: AAH57595.1.
CCDSiCCDS27118.1. [Q924T7-1]
RefSeqiNP_919327.2. NM_194346.2. [Q924T7-1]
UniGeneiMm.23748.

Genome annotation databases

EnsembliENSMUST00000019443; ENSMUSP00000019443; ENSMUSG00000047098. [Q924T7-1]
GeneIDi268749.
KEGGimmu:268749.
UCSCiuc007tzi.1. mouse. [Q924T7-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB053120 Genomic DNA. Translation: BAB47406.1.
BC057595 mRNA. Translation: AAH57595.1.
CCDSiCCDS27118.1. [Q924T7-1]
RefSeqiNP_919327.2. NM_194346.2. [Q924T7-1]
UniGeneiMm.23748.

3D structure databases

ProteinModelPortaliQ924T7.
SMRiQ924T7. Positions 5-177, 345-373, 476-620, 684-1066.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi234548. 12 interactions.
DIPiDIP-49579N.
IntActiQ924T7. 4 interactions.
STRINGi10090.ENSMUSP00000019443.

PTM databases

PhosphoSiteiQ924T7.

Proteomic databases

EPDiQ924T7.
MaxQBiQ924T7.
PaxDbiQ924T7.
PRIDEiQ924T7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000019443; ENSMUSP00000019443; ENSMUSG00000047098. [Q924T7-1]
GeneIDi268749.
KEGGimmu:268749.
UCSCiuc007tzi.1. mouse. [Q924T7-1]

Organism-specific databases

CTDi55072.
MGIiMGI:1934704. Rnf31.

Phylogenomic databases

eggNOGiKOG1812. Eukaryota.
ENOG410XS1H. LUCA.
GeneTreeiENSGT00530000064112.
HOVERGENiHBG063678.
InParanoidiQ924T7.
KOiK11974.
OMAiRTNDPEY.
OrthoDBiEOG7BW0HN.
PhylomeDBiQ924T7.
TreeFamiTF350529.

Enzyme and pathway databases

ReactomeiR-MMU-5357905. Regulation of TNFR1 signaling.
R-MMU-5357956. TNFR1-induced NFkappaB signaling pathway.

Miscellaneous databases

NextBioi392473.
PROiQ924T7.
SOURCEiSearch...

Gene expression databases

BgeeiQ924T7.
CleanExiMM_RNF31.
ExpressionAtlasiQ924T7. baseline and differential.
GenevisibleiQ924T7. MM.

Family and domain databases

InterProiIPR002867. IBR_dom.
IPR018997. PUB_domain.
IPR026254. RNF31.
IPR032065. RNF31-UBA.
IPR015940. UBA.
IPR001876. Znf_RanBP2.
IPR017907. Znf_RING_CS.
[Graphical view]
PANTHERiPTHR16004. PTHR16004. 1 hit.
PfamiPF16678. HOIP-UBA. 1 hit.
PF01485. IBR. 1 hit.
PF09409. PUB. 1 hit.
[Graphical view]
SMARTiSM00647. IBR. 2 hits.
SM00547. ZnF_RBZ. 3 hits.
[Graphical view]
SUPFAMiSSF143503. SSF143503. 1 hit.
SSF90209. SSF90209. 2 hits.
PROSITEiPS50030. UBA. 1 hit.
PS01358. ZF_RANBP2_1. 3 hits.
PS50199. ZF_RANBP2_2. 1 hit.
PS00518. ZF_RING_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence analysis of the ~35-kb segment containing interferon-gamma-inducible mouse proteasome activator genes."
    Yawata M., Murata S., Tanaka K., Ishigatsubo Y., Kasahara M.
    Immunogenetics 53:119-129(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 2).
    Strain: 129/SvJ.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Brain.
  3. "A putative ariadne-like E3 ubiquitin ligase (PAUL) that interacts with the muscle-specific kinase (MuSK)."
    Bromann P.A., Weiner J.A., Apel E.D., Lewis R.M., Sanes J.R.
    Gene Expr. Patterns 4:77-84(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MUSK, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Heart, Lung and Pancreas.
  5. Cited for: INTERACTION WITH FAM105B.

Entry informationi

Entry nameiRNF31_MOUSE
AccessioniPrimary (citable) accession number: Q924T7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2004
Last sequence update: March 15, 2004
Last modified: May 11, 2016
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.