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Protein

Beta-1,3-N-acetylglucosaminyltransferase lunatic fringe

Gene

Lfng

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Glycosyltransferase that initiates the elongation of O-linked fucose residues attached to EGF-like repeats in the extracellular domain of Notch molecules inhibiting Jagged mediated Notch signaling. Essential mediator of somite segmentation and patterning (By similarity).By similarity

Catalytic activityi

Transfers a beta-D-GlcNAc residue from UDP-D-GlcNAc to the fucose residue of a fucosylated protein acceptor.

Cofactori

Mn2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei128SubstrateBy similarity1
Binding sitei200SubstrateBy similarity1
Metal bindingi201ManganeseBy similarity1
Active sitei289By similarity1
Metal bindingi313ManganeseBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Glycosyltransferase, Transferase

Keywords - Ligandi

Manganese, Metal-binding

Protein family/group databases

CAZyiGT31. Glycosyltransferase Family 31.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-1,3-N-acetylglucosaminyltransferase lunatic fringe (EC:2.4.1.222)
Alternative name(s):
O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase
Gene namesi
Name:Lfng
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 12

Organism-specific databases

RGDi620587. Lfng.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 8CytoplasmicSequence analysis8
Transmembranei9 – 29Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini30 – 378LumenalSequence analysisAdd BLAST349

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002191781 – 378Beta-1,3-N-acetylglucosaminyltransferase lunatic fringeAdd BLAST378

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi166N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi167 ↔ 178By similarity
Disulfide bondi196 ↔ 259By similarity
Disulfide bondi363 ↔ 372By similarity

Post-translational modificationi

A soluble form may be derived from the membrane form by proteolytic processing.Curated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei85 – 86Cleavage; by furin-like proteaseSequence analysis2

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ924T4.
PRIDEiQ924T4.

PTM databases

PhosphoSitePlusiQ924T4.

Expressioni

Gene expression databases

BgeeiENSRNOG00000001250.
GenevisibleiQ924T4. RN.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000001682.

Structurei

3D structure databases

ProteinModelPortaliQ924T4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 31 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410II9W. Eukaryota.
ENOG410XS8Y. LUCA.
GeneTreeiENSGT00390000009913.
HOGENOMiHOG000046678.
HOVERGENiHBG007986.
InParanoidiQ924T4.
KOiK05948.
OMAiFENKRNI.
OrthoDBiEOG091G08XO.
PhylomeDBiQ924T4.
TreeFamiTF324207.

Family and domain databases

InterProiIPR017374. Fringe.
IPR003378. Fringe-like.
[Graphical view]
PfamiPF02434. Fringe. 1 hit.
[Graphical view]
PIRSFiPIRSF038073. B-acetylgalactosaminyltfrase. 1 hit.

Sequencei

Sequence statusi: Complete.

Q924T4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLQRCGRRLL LALVGALLAC LLVLTADPPP TPVPAERGRR ALRSLAGSSG
60 70 80 90 100
AAPGPGSRAA VDPGVLVREV HSLSEYFSLL TRARRDADPP PGVASRQGDG
110 120 130 140 150
HPRPPAEVLS PRDVFIAVKT TRKFHRARLD LLFETWISRH KEMTFIFTDG
160 170 180 190 200
EDEALAKHTG NVVLTNCSAA HSRQALSCKM AVEYDRFIES GKKWFCHVDD
210 220 230 240 250
DNYVNLRALL RLLASYPHTQ DVYIGKPSLD RPIQATERIS EHRVRPVHFW
260 270 280 290 300
FATGGAGFCI SRGLALKMGP WASGGHFMST AERIRLPDDC TIGYIVEALL
310 320 330 340 350
GVPLIRSGLF HSHLENLQQV PTTELHEQVT LSYGMFENKR NAVHIKGPFS
360 370
VEADPSRFRS IHCHLYPDTP WCPRTAIL
Length:378
Mass (Da):41,958
Last modified:December 1, 2001 - v1
Checksum:i482A68ED60499066
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB054539 mRNA. Translation: BAB63256.1.
BC070933 mRNA. Translation: AAH70933.1.
RefSeqiNP_596884.1. NM_133393.2.
UniGeneiRn.127809.

Genome annotation databases

EnsembliENSRNOT00000001682; ENSRNOP00000001682; ENSRNOG00000001250.
GeneIDi170905.
KEGGirno:170905.
UCSCiRGD:620587. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB054539 mRNA. Translation: BAB63256.1.
BC070933 mRNA. Translation: AAH70933.1.
RefSeqiNP_596884.1. NM_133393.2.
UniGeneiRn.127809.

3D structure databases

ProteinModelPortaliQ924T4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000001682.

Protein family/group databases

CAZyiGT31. Glycosyltransferase Family 31.

PTM databases

PhosphoSitePlusiQ924T4.

Proteomic databases

PaxDbiQ924T4.
PRIDEiQ924T4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000001682; ENSRNOP00000001682; ENSRNOG00000001250.
GeneIDi170905.
KEGGirno:170905.
UCSCiRGD:620587. rat.

Organism-specific databases

CTDi3955.
RGDi620587. Lfng.

Phylogenomic databases

eggNOGiENOG410II9W. Eukaryota.
ENOG410XS8Y. LUCA.
GeneTreeiENSGT00390000009913.
HOGENOMiHOG000046678.
HOVERGENiHBG007986.
InParanoidiQ924T4.
KOiK05948.
OMAiFENKRNI.
OrthoDBiEOG091G08XO.
PhylomeDBiQ924T4.
TreeFamiTF324207.

Miscellaneous databases

PROiQ924T4.

Gene expression databases

BgeeiENSRNOG00000001250.
GenevisibleiQ924T4. RN.

Family and domain databases

InterProiIPR017374. Fringe.
IPR003378. Fringe-like.
[Graphical view]
PfamiPF02434. Fringe. 1 hit.
[Graphical view]
PIRSFiPIRSF038073. B-acetylgalactosaminyltfrase. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiLFNG_RAT
AccessioniPrimary (citable) accession number: Q924T4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 10, 2003
Last sequence update: December 1, 2001
Last modified: November 2, 2016
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.