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Q924S7

- SPRE2_MOUSE

UniProt

Q924S7 - SPRE2_MOUSE

Protein

Sprouty-related, EVH1 domain-containing protein 2

Gene

Spred2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 90 (01 Oct 2014)
      Sequence version 1 (01 Dec 2001)
      Previous versions | rss
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    Functioni

    Tyrosine kinase substrate that inhibits growth-factor-mediated activation of MAP kinase.1 Publication

    GO - Molecular functioni

    1. protein binding Source: UniProtKB
    2. protein kinase binding Source: BHF-UCL
    3. protein serine/threonine kinase inhibitor activity Source: BHF-UCL
    4. stem cell factor receptor binding Source: MGI

    GO - Biological processi

    1. inactivation of MAPK activity Source: MGI
    2. multicellular organismal development Source: InterPro
    3. negative regulation of peptidyl-threonine phosphorylation Source: BHF-UCL
    4. positive regulation of DNA damage response, signal transduction by p53 class mediator Source: BHF-UCL
    5. regulation of protein deacetylation Source: BHF-UCL

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sprouty-related, EVH1 domain-containing protein 2
    Short name:
    Spred-2
    Gene namesi
    Name:Spred2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 11

    Organism-specific databases

    MGIiMGI:2150019. Spred2.

    Subcellular locationi

    Membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity. Cytoplasmic vesiclesecretory vesicle membrane Curated; Peripheral membrane protein Curated; Cytoplasmic side Curated. Cytoplasm 1 Publication
    Note: Especially found in the cytoplasm of the stratum spinosum cells called membrane-coated granules.By similarity

    GO - Cellular componenti

    1. cytoplasmic vesicle Source: MGI
    2. plasma membrane Source: MGI
    3. transport vesicle membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Cytoplasmic vesicle, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 410410Sprouty-related, EVH1 domain-containing protein 2PRO_0000076911Add
    BLAST

    Post-translational modificationi

    Phosphorylated on tyrosine. Phosphorylation of Tyr-224 and Tyr-227 are required for ubiquitination By similarity.By similarity
    Ubiquitinated; leading to degradation by the proteasome.By similarity

    Keywords - PTMi

    Phosphoprotein, Ubl conjugation

    Proteomic databases

    PaxDbiQ924S7.
    PRIDEiQ924S7.

    PTM databases

    PhosphoSiteiQ924S7.

    Expressioni

    Tissue specificityi

    Predominantly expressed in lung, liver and testis. In testis, it is specially found in mature spermatids projecting into the lumen of the seminiferous. Strongly expressed in glandular epithelia. Also expressed in embryonic tissues such as heart, lung, liver and brain.2 Publications

    Gene expression databases

    ArrayExpressiQ924S7.
    BgeeiQ924S7.
    CleanExiMM_SPRED2.
    GenevestigatoriQ924S7.

    Interactioni

    Subunit structurei

    Interacts with ras.1 Publication

    Protein-protein interaction databases

    BioGridi227830. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ924S7.
    SMRiQ924S7. Positions 1-124.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini5 – 122118WH1PROSITE-ProRule annotationAdd
    BLAST
    Domaini197 – 25256KBDPROSITE-ProRule annotationAdd
    BLAST
    Domaini300 – 408109SPRPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 KBD domain.PROSITE-ProRule annotation
    Contains 1 SPR (sprouty) domain.PROSITE-ProRule annotation
    Contains 1 WH1 domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG277322.
    GeneTreeiENSGT00730000110272.
    HOGENOMiHOG000220886.
    HOVERGENiHBG057556.
    KOiK04703.

    Family and domain databases

    Gene3Di2.30.29.30. 1 hit.
    InterProiIPR023337. KBD.
    IPR011993. PH_like_dom.
    IPR007875. Sprouty.
    IPR000697. WH1/EVH1.
    [Graphical view]
    PfamiPF05210. Sprouty. 1 hit.
    PF00568. WH1. 1 hit.
    [Graphical view]
    PROSITEiPS51488. KBD. 1 hit.
    PS51227. SPR. 1 hit.
    PS50229. WH1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q924S7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTEETHPDDD SYIVRVKAVV MTRDDSSGGW FPQEGGGISR VGVCKVMHPE    50
    GNGRSGFLIH GERQKDKLVV LECYVRKDLV YTKANPTFHH WKVDNRKFGL 100
    TFQSPADARA FDRGVRKAIE DLIEGSTTSS STLHNEAELG DDDVFTTATD 150
    SSSNSSQKRE PTTRTISSPT SCEHRKIYTL DPYPMDHYHP DQRLPRSYPQ 200
    VTFPEDDEEI VRINPREKIW MTGYEDYRHA PVRGKYLDTT EDADSYVRFA 250
    KGEVPKHEYT YPYVDSSDFG FGEDPKGSVI KTQPPRAKSR RRKENGERSR 300
    CVYCRDMFNH EENRRGHCQD APDAVRTCIR RVSCMWCADS MLYHCMSDPE 350
    GDYTDPCSCD TSDEKFCLRW MALIALSFLA PCMCCYLPLR ACHRCGVMCR 400
    CCGGKHKAAA 410
    Length:410
    Mass (Da):46,794
    Last modified:December 1, 2001 - v1
    Checksum:i8A652D06CE8D3BB1
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB063496 mRNA. Translation: BAB62849.1.
    AK036430 mRNA. Translation: BAC29425.1.
    BC066013 mRNA. Translation: AAH66013.1.
    CCDSiCCDS24454.1.
    RefSeqiNP_277058.1. NM_033523.4.
    UniGeneiMm.266627.

    Genome annotation databases

    EnsembliENSMUST00000093298; ENSMUSP00000090987; ENSMUSG00000045671.
    GeneIDi114716.
    KEGGimmu:114716.
    UCSCiuc007icr.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB063496 mRNA. Translation: BAB62849.1 .
    AK036430 mRNA. Translation: BAC29425.1 .
    BC066013 mRNA. Translation: AAH66013.1 .
    CCDSi CCDS24454.1.
    RefSeqi NP_277058.1. NM_033523.4.
    UniGenei Mm.266627.

    3D structure databases

    ProteinModelPortali Q924S7.
    SMRi Q924S7. Positions 1-124.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 227830. 1 interaction.

    PTM databases

    PhosphoSitei Q924S7.

    Proteomic databases

    PaxDbi Q924S7.
    PRIDEi Q924S7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000093298 ; ENSMUSP00000090987 ; ENSMUSG00000045671 .
    GeneIDi 114716.
    KEGGi mmu:114716.
    UCSCi uc007icr.1. mouse.

    Organism-specific databases

    CTDi 200734.
    MGIi MGI:2150019. Spred2.

    Phylogenomic databases

    eggNOGi NOG277322.
    GeneTreei ENSGT00730000110272.
    HOGENOMi HOG000220886.
    HOVERGENi HBG057556.
    KOi K04703.

    Miscellaneous databases

    ChiTaRSi SPRED2. mouse.
    NextBioi 368741.
    PROi Q924S7.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q924S7.
    Bgeei Q924S7.
    CleanExi MM_SPRED2.
    Genevestigatori Q924S7.

    Family and domain databases

    Gene3Di 2.30.29.30. 1 hit.
    InterProi IPR023337. KBD.
    IPR011993. PH_like_dom.
    IPR007875. Sprouty.
    IPR000697. WH1/EVH1.
    [Graphical view ]
    Pfami PF05210. Sprouty. 1 hit.
    PF00568. WH1. 1 hit.
    [Graphical view ]
    PROSITEi PS51488. KBD. 1 hit.
    PS51227. SPR. 1 hit.
    PS50229. WH1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, INTERACTION WITH RAS, TISSUE SPECIFICITY, FUNCTION.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Bone.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Brain.
    4. "Expression and subcellular localization of Spred proteins in mouse and human tissues."
      Engelhardt C.M., Bundschu K., Messerschmitt M., Renne T., Walter U., Reinhard M., Schuh K.
      Histochem. Cell Biol. 122:527-538(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    5. "The phagosomal proteome in interferon-gamma-activated macrophages."
      Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
      Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiSPRE2_MOUSE
    AccessioniPrimary (citable) accession number: Q924S7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 4, 2005
    Last sequence update: December 1, 2001
    Last modified: October 1, 2014
    This is version 90 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3