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Q924S7 (SPRE2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 89. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Sprouty-related, EVH1 domain-containing protein 2

Short name=Spred-2
Gene names
Name:Spred2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length410 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Tyrosine kinase substrate that inhibits growth-factor-mediated activation of MAP kinase. Ref.1

Subunit structure

Interacts with ras. Ref.1

Subcellular location

Membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Cytoplasmic vesiclesecretory vesicle membrane; Peripheral membrane protein; Cytoplasmic side Potential. Cytoplasm. Note: Especially found in the cytoplasm of the stratum spinosum cells called membrane-coated granules By similarity. Ref.1

Tissue specificity

Predominantly expressed in lung, liver and testis. In testis, it is specially found in mature spermatids projecting into the lumen of the seminiferous. Strongly expressed in glandular epithelia. Also expressed in embryonic tissues such as heart, lung, liver and brain. Ref.1 Ref.4

Post-translational modification

Phosphorylated on tyrosine. Phosphorylation of Tyr-224 and Tyr-227 are required for ubiquitination By similarity.

Ubiquitinated; leading to degradation by the proteasome By similarity.

Sequence similarities

Contains 1 KBD domain.

Contains 1 SPR (sprouty) domain.

Contains 1 WH1 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 410410Sprouty-related, EVH1 domain-containing protein 2
PRO_0000076911

Regions

Domain5 – 122118WH1
Domain197 – 25256KBD
Domain300 – 408109SPR

Sequences

Sequence LengthMass (Da)Tools
Q924S7 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: 8A652D06CE8D3BB1

FASTA41046,794
        10         20         30         40         50         60 
MTEETHPDDD SYIVRVKAVV MTRDDSSGGW FPQEGGGISR VGVCKVMHPE GNGRSGFLIH 

        70         80         90        100        110        120 
GERQKDKLVV LECYVRKDLV YTKANPTFHH WKVDNRKFGL TFQSPADARA FDRGVRKAIE 

       130        140        150        160        170        180 
DLIEGSTTSS STLHNEAELG DDDVFTTATD SSSNSSQKRE PTTRTISSPT SCEHRKIYTL 

       190        200        210        220        230        240 
DPYPMDHYHP DQRLPRSYPQ VTFPEDDEEI VRINPREKIW MTGYEDYRHA PVRGKYLDTT 

       250        260        270        280        290        300 
EDADSYVRFA KGEVPKHEYT YPYVDSSDFG FGEDPKGSVI KTQPPRAKSR RRKENGERSR 

       310        320        330        340        350        360 
CVYCRDMFNH EENRRGHCQD APDAVRTCIR RVSCMWCADS MLYHCMSDPE GDYTDPCSCD 

       370        380        390        400        410 
TSDEKFCLRW MALIALSFLA PCMCCYLPLR ACHRCGVMCR CCGGKHKAAA 

« Hide

References

« Hide 'large scale' references
[1]"Spred is a Sprouty-related suppressor of Ras signalling."
Wakioka T., Sasaki A., Kato R., Shouda T., Matsumoto A., Miyoshi K., Tsuneoka M., Komiya S., Baron R., Yoshimura A.
Nature 412:647-651(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, INTERACTION WITH RAS, TISSUE SPECIFICITY, FUNCTION.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Bone.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6.
Tissue: Brain.
[4]"Expression and subcellular localization of Spred proteins in mouse and human tissues."
Engelhardt C.M., Bundschu K., Messerschmitt M., Renne T., Walter U., Reinhard M., Schuh K.
Histochem. Cell Biol. 122:527-538(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[5]"The phagosomal proteome in interferon-gamma-activated macrophages."
Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB063496 mRNA. Translation: BAB62849.1.
AK036430 mRNA. Translation: BAC29425.1.
BC066013 mRNA. Translation: AAH66013.1.
CCDSCCDS24454.1.
RefSeqNP_277058.1. NM_033523.4.
UniGeneMm.266627.

3D structure databases

ProteinModelPortalQ924S7.
SMRQ924S7. Positions 1-124.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid227830. 1 interaction.

PTM databases

PhosphoSiteQ924S7.

Proteomic databases

PaxDbQ924S7.
PRIDEQ924S7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000093298; ENSMUSP00000090987; ENSMUSG00000045671.
GeneID114716.
KEGGmmu:114716.
UCSCuc007icr.1. mouse.

Organism-specific databases

CTD200734.
MGIMGI:2150019. Spred2.

Phylogenomic databases

eggNOGNOG277322.
GeneTreeENSGT00730000110272.
HOGENOMHOG000220886.
HOVERGENHBG057556.
KOK04703.

Gene expression databases

ArrayExpressQ924S7.
BgeeQ924S7.
CleanExMM_SPRED2.
GenevestigatorQ924S7.

Family and domain databases

Gene3D2.30.29.30. 1 hit.
InterProIPR023337. KBD.
IPR011993. PH_like_dom.
IPR007875. Sprouty.
IPR000697. WH1/EVH1.
[Graphical view]
PfamPF05210. Sprouty. 1 hit.
PF00568. WH1. 1 hit.
[Graphical view]
PROSITEPS51488. KBD. 1 hit.
PS51227. SPR. 1 hit.
PS50229. WH1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSSPRED2. mouse.
NextBio368741.
PROQ924S7.
SOURCESearch...

Entry information

Entry nameSPRE2_MOUSE
AccessionPrimary (citable) accession number: Q924S7
Entry history
Integrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: December 1, 2001
Last modified: July 9, 2014
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot