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Q924N5 (ACBG1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Long-chain-fatty-acid--CoA ligase ACSBG1

EC=6.2.1.3
Alternative name(s):
Acyl-CoA synthetase bubblegum family member 1
Gonadotropin-regulated long chain acyl CoA synthetase
Short name=GR-LACS
Gene names
Name:Acsbg1
Synonyms:Grlacs
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length721 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Mediates activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Able to activate long-chain fatty acids. Can activate diverse saturated, monosaturated and polyunsaturated fatty acids. Ref.1

Catalytic activity

ATP + a long-chain fatty acid + CoA = AMP + diphosphate + an acyl-CoA. Ref.1

Subcellular location

Cytoplasm. Cytoplasmic vesicle By similarity. Microsome By similarity. Endoplasmic reticulum By similarity Ref.1.

Tissue specificity

Present in testis, at a lower level in brain, and at a very low level in ovary. Not detected in other tissues. tested. Present in Leydig cells of the adult testis and to a lesser degree in the seminiferous tubules in spermatogonia and Sertoli cells (at protein level). Ref.1 Ref.2

Induction

Down-regulated by gonadotropin. Ref.1

Sequence similarities

Belongs to the ATP-dependent AMP-binding enzyme family. Bubblegum subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 721721Long-chain-fatty-acid--CoA ligase ACSBG1
PRO_0000315811

Regions

Nucleotide binding279 – 2879ATP By similarity
Nucleotide binding469 – 4746ATP By similarity

Sites

Binding site5471ATP By similarity
Binding site5621ATP By similarity
Binding site6981ATP By similarity

Amino acid modifications

Modified residue6551Phosphotyrosine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q924N5 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: 600DC8F87546DA32

FASTA72180,519
        10         20         30         40         50         60 
MPRSSEAGYC CLSRDSNMPD SRDDQQQGAS MGTSPDNSQT SSLIDGRTLS KESPSHGLEL 

        70         80         90        100        110        120 
SAPEKARAAS LDASEEALWT TRADGRVRLR LEPFCTQLPY TVHQMFYEAL DKYGNLSALG 

       130        140        150        160        170        180 
FKRKDKWERI SYYQYYLIAR KVAKGFLKLG LERAHSVAIL GFNSPEWFFS AVGTVFAGGI 

       190        200        210        220        230        240 
VTGIYTTSSP EACQYIAHDC RANVIVVDTQ KQLEKILKIW KDLPHLKAVV IYQEPPPKKM 

       250        260        270        280        290        300 
ANVYTMEELI ELGQEVPEEA LDAIIDTQQP NQCCVLVYTS GTTGNPKGVM LSQDNITWTA 

       310        320        330        340        350        360 
RYGSQAGDIQ PAEVQQEVVV SYLPLSHIAA QIYDLWTGIQ WGAQVCFADP DALKGTLVNT 

       370        380        390        400        410        420 
LREVEPTSHM GVPRVWEKIM ERIQEVAAQS GFIRRKMLLW AMSVTLEQNL TCPSNDLKPF 

       430        440        450        460        470        480 
TSRLADYLVL ARVRQALGFA KCQKNFYGAA PMTAETQRFF LGLNIRLYAG YGLSESTGPH 

       490        500        510        520        530        540 
FMSSPYNYRL YSSGRVVPGC RVKLVNQDAD GIGEICLWGR TIFMGYLNME DKTHEAIDSE 

       550        560        570        580        590        600 
GWLHTGDMGR LDDDGFLYIT GRLKELIITA GGENVPPVPI EEAVKMELPI ISSAMLIGDQ 

       610        620        630        640        650        660 
RKFLSMLLTL KCTLNPETSE PTDNLTEQAV EFCQRVGSKA STVSEIVGQK DEAVYQAIHE 

       670        680        690        700        710        720 
GIQRVNANAA ARPYHIQKWA ILERDFSISG GELGPTMKLK RLTVLEKYKD IIDSFYQEQK 


Q 

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References

[1]"Cloning and characterization of a hormonally regulated rat long chain acyl-CoA synthetase."
Tang P.-Z., Tsai-Morris C.-H., Dufau M.L.
Proc. Natl. Acad. Sci. U.S.A. 98:6581-6586(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, ENZYME ACTIVITY, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION.
[2]"Tissue-cell- and species-specific expression of gonadotropin-regulated long chain acyl-CoA synthetase (GR-LACS) in gonads, adrenal and brain. Identification of novel forms in the brain."
Li J., Sheng Y., Tang P.-Z., Tsai-Morris C.-H., Dufau M.L.
J. Steroid Biochem. Mol. Biol. 98:207-217(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF208125 mRNA. Translation: AAG35729.1.
RefSeqNP_599216.1. NM_134389.1.
UniGeneRn.17672.

3D structure databases

ProteinModelPortalQ924N5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid251236. 1 interaction.
STRING10116.ENSRNOP00000016104.

PTM databases

PhosphoSiteQ924N5.

2D gel databases

World-2DPAGE0004:Q924N5.

Proteomic databases

PaxDbQ924N5.
PRIDEQ924N5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000016104; ENSRNOP00000016104; ENSRNOG00000011381.
GeneID171410.
KEGGrno:171410.
UCSCRGD:708557. rat.

Organism-specific databases

CTD23205.
RGD708557. Acsbg1.

Phylogenomic databases

eggNOGCOG1022.
GeneTreeENSGT00690000101725.
HOGENOMHOG000230004.
HOVERGENHBG054660.
InParanoidQ924N5.
KOK15013.
OMATQQPNQC.
OrthoDBEOG7B5WVB.
TreeFamTF354286.

Enzyme and pathway databases

BRENDA6.2.1.3. 3474.

Gene expression databases

GenevestigatorQ924N5.

Family and domain databases

InterProIPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamPF00501. AMP-binding. 1 hit.
[Graphical view]
PROSITEPS00455. AMP_BINDING. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio622288.
PROQ924N5.

Entry information

Entry nameACBG1_RAT
AccessionPrimary (citable) accession number: Q924N5
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: December 1, 2001
Last modified: February 19, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families