Reviewed, UniProtKB/Swiss-Prot Q924M7 (MPI_MOUSE)

Names and origin

Protein names

Recommended name:
    Mannose-6-phosphate isomerase
    EC=5.3.1.8
Alternative name(s):
    Phosphomannose isomerase
      Short name=PMI
    Phosphohexomutase

Gene names

Name:	Mpi
Synonyms:	Mpi1, Pmi

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	423 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions By similarity.
Catalytic activity	D-mannose 6-phosphate = D-fructose 6-phosphate.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	Nucleotide-sugar biosynthesis; GDP-alpha-D-mannose biosynthesis; alpha-D-mannose 1-phosphate from D-fructose 6-phosphate: step 1/2.
Subcellular location	Cytoplasm. Ref.1
Sequence similarities	Belongs to the mannose-6-phosphate isomerase type 1 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding Zinc
Molecular function	Isomerase
Gene Ontology (GO)
Biological process	mannose metabolic process Inferred by curator. Source: MGI
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	mannose-6-phosphate isomerase activity Inferred from direct assay. Source: MGI zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 423	423	Mannose-6-phosphate isomerase		PRO_0000194237
Sites
	Active site	295	1	By similarity
	Metal binding	110	1	Zinc By similarity
	Metal binding	112	1	Zinc By similarity
	Metal binding	137	1	Zinc By similarity
	Metal binding	276	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q924M7-1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 3A49506D1249B670	FASTA	423	46,575
	10 20 30 40 50 60 MASPRVFPLS CVVQQYAWGK VGSKSEVACL LASSDPLAQI SEDKPYAELW MGTHPRGDAK 70 80 90 100 110 120 ILDNRISQKT LGQWIAENPD CLGSKVKNTF NGKLPFLFKV LSVDTALSIQ AHPNKELAEK 130 140 150 160 170 180 LHLQAPEHYP DANHKPEMAI ALTSFQGLCG FRPVEEIVTF MKKVPEFQLL IGDDATAQLK 190 200 210 220 230 240 ESVGGDTEAM ASALRNCFSH LMKSEKKVVV EQLNLLVKRI SQQVFDGNNM EDIYGKLLLQ 250 260 270 280 290 300 LHQQHPGDIG CFAIYFLNLL TLKPGEAMFL DANVPHAYLK GDCVECMACS DNTVRAGLTP 310 320 330 340 350 360 KFIDVPTLCE MLNYTPSPSN NRLFAPAQSQ DDPYLSIYDP PVPDFTVMKM EVPSSVTEYK 370 380 390 400 410 420 VSTLDSASIL LMVQGTVTAI IPSAHAEIPL YRGGVLFIAA NESVLLKITV PKDLLIFRAC CLL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning, gene organization, and expression of mouse Mpi encoding phosphomannose isomerase." Davis J.A., Wu X.H., Wang L., DeRossi C., Westphal V., Wu R., Alton G., Srikrishna G., Freeze H.H. Glycobiology 12:435-442(2002) [PubMed: 12122025] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Eye.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF244360 mRNA. Translation: AAK69388.1. BC051163 mRNA. Translation: AAH51163.1.
IPI	IPI00125807.
RefSeq	NP_080113.1.
UniGene	Mm.247218
3D structure databases
HSSP	HSSP built from PDB template 1PMI based on UniProtKB P34948.
ModBase	Search...
Protein-protein interaction databases
STRING	Q924M7.
2-D gel databases
REPRODUCTION-2DPAGE	Q924M7.
Proteomic databases
PRIDE	Q924M7.
Genome annotation databases
Ensembl	ENSMUST00000034856; ENSMUSP00000034856; ENSMUSG00000032306; Mus musculus. [Genome view]
GeneID	110119.
KEGG	mmu:110119.
NMPDR	fig|10090.3.peg.20272.
UCSC	uc009pvd.1. mouse.
Organism-specific databases
CTD	110119.
MGI	MGI:97075. Mpi.
Phylogenomic databases
HOGENOM	Q924M7.
HOVERGEN	Q924M7.
OMA	MGSNSEV.
Enzyme and pathway databases
BRENDA	5.3.1.8. 244.
Gene expression databases
ArrayExpress	Q924M7.
Bgee	Q924M7.
CleanEx	MM_MPI.
Genevestigator	Q924M7.
GermOnline	ENSMUSG00000032306. Mus musculus.
Family and domain databases
InterPro	IPR001250. Man6P_Isoase-1. IPR016305. Mannose-6-P_Isomerase. IPR018050. Pmannose_isomerase-type1_CS. IPR014710. RmlC-like_jellyroll. [Graphical view]
Gene3D	G3DSA:2.60.120.10. RmlC-like_jellyroll. 2 hits.
Pfam	PF01238. PMI_typeI. 1 hit. [Graphical view]
PIRSF	PIRSF001480. Mannose-6-phosphate_isomerase. 1 hit.
PRINTS	PR00714. MAN6PISMRASE.
TIGRFAMs	TIGR00218. manA. 1 hit.
PROSITE	PS00965. PMI_I_1. 1 hit. PS00966. PMI_I_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	363359.
SOURCE	Search...

Entry information

Entry name

MPI_MOUSE

Accession

Primary (citable) accession number: Q924M7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 12, 2005
Last sequence update:	December 1, 2001
Last modified:	November 3, 2009
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)