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Protein

Mannose-6-phosphate isomerase

Gene

Mpi

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions.By similarity

Catalytic activityi

D-mannose 6-phosphate = D-fructose 6-phosphate.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi110 – 1101ZincBy similarity
Metal bindingi112 – 1121ZincBy similarity
Metal bindingi137 – 1371ZincBy similarity
Metal bindingi276 – 2761ZincBy similarity
Active sitei295 – 2951By similarity

GO - Molecular functioni

  1. mannose-6-phosphate isomerase activity Source: MGI
  2. zinc ion binding Source: InterPro

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
  2. GDP-mannose biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_328870. Synthesis of GDP-mannose.
UniPathwayiUPA00126; UER00423.

Names & Taxonomyi

Protein namesi
Recommended name:
Mannose-6-phosphate isomerase (EC:5.3.1.8)
Alternative name(s):
Phosphohexomutase
Phosphomannose isomerase
Short name:
PMI
Gene namesi
Name:Mpi
Synonyms:Mpi1, Pmi
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:97075. Mpi.

Subcellular locationi

  1. Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. extracellular vesicular exosome Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 423422Mannose-6-phosphate isomerasePRO_0000194237Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei102 – 1021PhosphoserineBy similarity
Modified residuei108 – 1081PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ924M7.
PaxDbiQ924M7.
PRIDEiQ924M7.

2D gel databases

REPRODUCTION-2DPAGEQ924M7.

PTM databases

PhosphoSiteiQ924M7.

Expressioni

Gene expression databases

BgeeiQ924M7.
CleanExiMM_MPI.
ExpressionAtlasiQ924M7. baseline and differential.
GenevestigatoriQ924M7.

Interactioni

Protein-protein interaction databases

IntActiQ924M7. 1 interaction.
MINTiMINT-4123108.
STRINGi10090.ENSMUSP00000034856.

Structurei

3D structure databases

ProteinModelPortaliQ924M7.
SMRiQ924M7. Positions 2-406.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1482.
GeneTreeiENSGT00390000016075.
HOGENOMiHOG000241277.
HOVERGENiHBG000367.
InParanoidiQ924M7.
KOiK01809.
OMAiVMKMEVP.
OrthoDBiEOG7GTT3M.
PhylomeDBiQ924M7.
TreeFamiTF312831.

Family and domain databases

Gene3Di2.60.120.10. 3 hits.
InterProiIPR001250. Man6P_Isoase-1.
IPR016305. Mannose-6-P_Isomerase.
IPR018050. Pmannose_isomerase-type1_CS.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PANTHERiPTHR10309. PTHR10309. 1 hit.
PfamiPF01238. PMI_typeI. 1 hit.
[Graphical view]
PIRSFiPIRSF001480. Mannose-6-phosphate_isomerase. 1 hit.
PRINTSiPR00714. MAN6PISMRASE.
SUPFAMiSSF51182. SSF51182. 1 hit.
TIGRFAMsiTIGR00218. manA. 1 hit.
PROSITEiPS00965. PMI_I_1. 1 hit.
PS00966. PMI_I_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q924M7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASPRVFPLS CVVQQYAWGK VGSKSEVACL LASSDPLAQI SEDKPYAELW
60 70 80 90 100
MGTHPRGDAK ILDNRISQKT LGQWIAENPD CLGSKVKNTF NGKLPFLFKV
110 120 130 140 150
LSVDTALSIQ AHPNKELAEK LHLQAPEHYP DANHKPEMAI ALTSFQGLCG
160 170 180 190 200
FRPVEEIVTF MKKVPEFQLL IGDDATAQLK ESVGGDTEAM ASALRNCFSH
210 220 230 240 250
LMKSEKKVVV EQLNLLVKRI SQQVFDGNNM EDIYGKLLLQ LHQQHPGDIG
260 270 280 290 300
CFAIYFLNLL TLKPGEAMFL DANVPHAYLK GDCVECMACS DNTVRAGLTP
310 320 330 340 350
KFIDVPTLCE MLNYTPSPSN NRLFAPAQSQ DDPYLSIYDP PVPDFTVMKM
360 370 380 390 400
EVPSSVTEYK VSTLDSASIL LMVQGTVTAI IPSAHAEIPL YRGGVLFIAA
410 420
NESVLLKITV PKDLLIFRAC CLL
Length:423
Mass (Da):46,575
Last modified:December 1, 2001 - v1
Checksum:i3A49506D1249B670
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF244360 mRNA. Translation: AAK69388.1.
BC051163 mRNA. Translation: AAH51163.1.
CCDSiCCDS40650.1.
RefSeqiNP_080113.1. NM_025837.2.
UniGeneiMm.247218.

Genome annotation databases

EnsembliENSMUST00000034856; ENSMUSP00000034856; ENSMUSG00000032306.
GeneIDi110119.
KEGGimmu:110119.
UCSCiuc009pvd.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF244360 mRNA. Translation: AAK69388.1.
BC051163 mRNA. Translation: AAH51163.1.
CCDSiCCDS40650.1.
RefSeqiNP_080113.1. NM_025837.2.
UniGeneiMm.247218.

3D structure databases

ProteinModelPortaliQ924M7.
SMRiQ924M7. Positions 2-406.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ924M7. 1 interaction.
MINTiMINT-4123108.
STRINGi10090.ENSMUSP00000034856.

PTM databases

PhosphoSiteiQ924M7.

2D gel databases

REPRODUCTION-2DPAGEQ924M7.

Proteomic databases

MaxQBiQ924M7.
PaxDbiQ924M7.
PRIDEiQ924M7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000034856; ENSMUSP00000034856; ENSMUSG00000032306.
GeneIDi110119.
KEGGimmu:110119.
UCSCiuc009pvd.1. mouse.

Organism-specific databases

CTDi4351.
MGIiMGI:97075. Mpi.

Phylogenomic databases

eggNOGiCOG1482.
GeneTreeiENSGT00390000016075.
HOGENOMiHOG000241277.
HOVERGENiHBG000367.
InParanoidiQ924M7.
KOiK01809.
OMAiVMKMEVP.
OrthoDBiEOG7GTT3M.
PhylomeDBiQ924M7.
TreeFamiTF312831.

Enzyme and pathway databases

UniPathwayiUPA00126; UER00423.
ReactomeiREACT_328870. Synthesis of GDP-mannose.

Miscellaneous databases

NextBioi363359.
PROiQ924M7.
SOURCEiSearch...

Gene expression databases

BgeeiQ924M7.
CleanExiMM_MPI.
ExpressionAtlasiQ924M7. baseline and differential.
GenevestigatoriQ924M7.

Family and domain databases

Gene3Di2.60.120.10. 3 hits.
InterProiIPR001250. Man6P_Isoase-1.
IPR016305. Mannose-6-P_Isomerase.
IPR018050. Pmannose_isomerase-type1_CS.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PANTHERiPTHR10309. PTHR10309. 1 hit.
PfamiPF01238. PMI_typeI. 1 hit.
[Graphical view]
PIRSFiPIRSF001480. Mannose-6-phosphate_isomerase. 1 hit.
PRINTSiPR00714. MAN6PISMRASE.
SUPFAMiSSF51182. SSF51182. 1 hit.
TIGRFAMsiTIGR00218. manA. 1 hit.
PROSITEiPS00965. PMI_I_1. 1 hit.
PS00966. PMI_I_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning, gene organization, and expression of mouse Mpi encoding phosphomannose isomerase."
    Davis J.A., Wu X.H., Wang L., DeRossi C., Westphal V., Wu R., Alton G., Srikrishna G., Freeze H.H.
    Glycobiology 12:435-442(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Eye.

Entry informationi

Entry nameiMPI_MOUSE
AccessioniPrimary (citable) accession number: Q924M7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 12, 2005
Last sequence update: December 1, 2001
Last modified: April 1, 2015
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.