Q924M5 (BOLL_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein boule-like

Gene names

Name:	Boll
Synonyms:	Boule

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	281 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Probable RNA-binding protein, which may be required during spermatogenesis. May act by binding to the 3'-UTR of mRNAs and regulating their translation By similarity.
Subunit structure	Interacts with DAZ1 and DAZL By similarity.
Subcellular location	Cytoplasm Ref.1.
Tissue specificity	Testis specific. Not expressed in early embryos, primoridal germ cells and spermatogonial cells. First expressed in the cytoplasm of spermatocytes and then persists through meiosis. Ref.1
Developmental stage	First expressed in stage III spermatocytes and peaks in late pachytene or diplotene stage spermatocytes. Expressed in secondary spermatocytes and early spermatids, then decreases until it is undetectable in spermatids. Ref.1
Sequence similarities	Belongs to the RRM DAZ family. Contains 1 DAZ-like domain. Contains 1 RRM (RNA recognition motif) domain.

Ontologies

Keywords
Biological process	Differentiation Spermatogenesis Translation regulation
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Ligand	RNA-binding
Molecular function	Developmental protein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW regulation of translation Inferred from electronic annotation. Source: UniProtKB-KW spermatogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q924M5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q924M5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → METESRAQSTNQT
     119-120: SS → MY
     121-281: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 281	281	Protein boule-like		PRO_0000081496
Regions
	Domain	33 – 110	78	RRM
	Domain	164 – 194	31	DAZ-like
Natural variations
	Alternative sequence	1	1	M → METESRAQSTNQT in isoform 2.		VSP_009447
	Alternative sequence	119 – 120	2	SS → MY in isoform 2.		VSP_009448
	Alternative sequence	121 – 281	161	Missing in isoform 2.		VSP_009449
Experimental info
	Sequence conflict	1	1	M → EFRCCRTESRAQSTNQT Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 1, 2004. Version 2. Checksum: 42C32EA6B0C21D10	FASTA	281	30,856
	10 20 30 40 50 60 MQTDSLSPSP NPVSPVPLNN PTSGPRYGTV IPNRIFVGGI DFKTNENDLR KFFSQYGSVK 70 80 90 100 110 120 EVKIVNDRAG VSKGYGFITF ETQEDAQKIL QEAEKLNYKD KKLNIGPAIR KQQVGIPRSS 130 140 150 160 170 180 LMPAAGTMYL TTSTGYPYTY HNGVAYFHTP EVTSVPPSWP SRSISSSPVM VAQPVYQQPA 190 200 210 220 230 240 YHYQAPAQCL PGQWQWGVPQ SPASSAPFLY LQPSEVIYQP VEIAQDGGCV PPPLSLMETS 250 260 270 280 VPEPYSDHGV QAAYHQVYAS SAIAMPAPMM QPEPIKVRSL F
	Isoform 2 [UniParc]. Checksum: 9308BC3C19A2E798	FASTA	132	14,768
	10 20 30 40 50 60 METESRAQST NQTQTDSLSP SPNPVSPVPL NNPTSGPRYG TVIPNRIFVG GIDFKTNEND 70 80 90 100 110 120 LRKFFSQYGS VKEVKIVNDR AGVSKGYGFI TFETQEDAQK ILQEAEKLNY KDKKLNIGPA 130 IRKQQVGIPR MY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A gene family required for human germ cell development evolved from an ancient meiotic gene conserved in metazoans." Xu E.Y., Moore F.L., Reijo Pera R.A. Proc. Natl. Acad. Sci. U.S.A. 98:7414-7419(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-46 (ISOFORM 1). Strain: C57BL/6J. Tissue: Testis.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF272859 mRNA. Translation: AAK69026.1. AK016125 mRNA. Translation: BAB30121.1. BY103870 mRNA. No translation available.
IPI	IPI00403643. IPI00886395.
RefSeq	NP_001106838.1. NM_001113367.1. NP_083543.2. NM_029267.3.
UniGene	Mm.483408.
3D structure databases
ProteinModelPortal	Q924M5.
SMR	Q924M5. Positions 28-120.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000084868.
PTM databases
PhosphoSite	Q924M5.
Proteomic databases
PaxDb	Q924M5.
PRIDE	Q924M5.
Protocols and materials databases
DNASU	75388.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000159398; ENSMUSP00000123814; ENSMUSG00000025977.
GeneID	75388.
KEGG	mmu:75388.
UCSC	uc007bal.1. mouse. uc007bam.1. mouse.
Organism-specific databases
CTD	66037.
MGI	MGI:1922638. Boll.
Phylogenomic databases
eggNOG	NOG260394.
GeneTree	ENSGT00530000063480.
HOGENOM	HOG000095250.
HOVERGEN	HBG048761.
InParanoid	Q924M5.
OrthoDB	EOG4MPHQQ.
Gene expression databases
ArrayExpress	Q924M5.
Bgee	Q924M5.
CleanEx	MM_BOLL.
Genevestigator	Q924M5.
GermOnline	ENSMUSG00000025977. Mus musculus.
Family and domain databases
Gene3D	3.30.70.330. 1 hit.
InterPro	IPR012677. Nucleotide-bd_a/b_plait. IPR000504. RRM_dom. [Graphical view]
Pfam	PF00076. RRM_1. 1 hit. [Graphical view]
SMART	SM00360. RRM. 1 hit. [Graphical view]
PROSITE	PS50102. RRM. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	342892.
SOURCE	Search...

Entry information

Entry name

BOLL_MOUSE

Accession

Primary (citable) accession number: Q924M5
Secondary accession number(s): Q9D4V2

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2004
Last sequence update:	March 1, 2004
Last modified:	May 1, 2013
This is version 87 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program