Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Mitogen-activated protein kinase kinase kinase kinase 3

Gene

Map4k3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.By similarity

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei45 – 451ATPPROSITE-ProRule annotation
Active sitei136 – 1361Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi22 – 309ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase kinase kinase kinase 3 (EC:2.7.11.1)
Alternative name(s):
Germinal center kinase-related protein kinase
Short name:
GLK
MAPK/ERK kinase kinase kinase 3
Short name:
MEK kinase kinase 3
Short name:
MEKKK 3
Gene namesi
Name:Map4k3
OrganismiRattus norvegicus (Rat)Imported
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi621280. Map4k3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 873873Mitogen-activated protein kinase kinase kinase kinase 3PRO_0000086279Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei329 – 3291PhosphoserineBy similarity
Modified residuei377 – 3771PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ924I2.
PRIDEiQ924I2.

PTM databases

PhosphoSiteiQ924I2.

Interactioni

Subunit structurei

Interacts with SH3GL2. Interaction appears to regulate MAP4K3-mediated JNK activation.1 Publication

Protein-protein interaction databases

BioGridi251030. 1 interaction.
MINTiMINT-199992.
STRINGi10116.ENSRNOP00000010366.

Structurei

3D structure databases

ProteinModelPortaliQ924I2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini16 – 273258Protein kinasePROSITE-ProRule annotationAdd
BLAST
Domaini535 – 846312CNHPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 CNH domain.PROSITE-ProRule annotationCurated
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0576. Eukaryota.
ENOG410XP9G. LUCA.
HOGENOMiHOG000230824.
HOVERGENiHBG036702.
InParanoidiQ924I2.
KOiK04406.

Family and domain databases

InterProiIPR001180. CNH_dom.
IPR011009. Kinase-like_dom.
IPR021160. MAPKKKK.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
[Graphical view]
PfamiPF00780. CNH. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
PIRSFiPIRSF038172. MAPKKKK. 1 hit.
SMARTiSM00036. CNH. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50219. CNH. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q924I2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNPGFDLSRR NPQEDFELIQ RIGSGTYGDV YKARNVNTGE LAAIKVIKLE
60 70 80 90 100
PGEDFAVVQQ EIIMMKDCKH ANIVAYFGSY LRRDKLWICM EFCGGGSLQD
110 120 130 140 150
IYHVTGPLSE LQIAYVSRET LQGLYYLHSK GKMHRDIKGA NILLTDNGHV
160 170 180 190 200
KLADFGVSAQ ITATIAKRKS FIGTPYWMAP EVAAVERKGG YNQLCDLWAV
210 220 230 240 250
GITAIELAEL QPPMFDLHPM RALFLMTKSN FQPPKLKDKL KWSNSFHHFV
260 270 280 290 300
KMALTKNPKK RPTAEKLLQH PFVTQPLTRS LAIELLDKVN NPDHSTYHDF
310 320 330 340 350
DDDDPEPLVA VPHRIPSTSR NVREEKTRSE INFGQVKFDP PLRKETEPHH
360 370 380 390 400
ELDLQLEYGQ GHQSNYFLGG NKSLLKSVEE ELHQRGHVAH LEDDEGDDDD
410 420 430 440 450
SKHSTLKAKV PPPLPPKPKS ISIPQDTHSS EDSNQGTIKR CPSSGSPAKP
460 470 480 490 500
SHVPPRPPPP RLPPQKPAVL GNGVSSFQLN GERDGSVHQQ QSEQRGTNLS
510 520 530 540 550
RKEKKDVPKP ISNGLPPTPK VHMGACFSKV FNGCPLKIHC ATSWINPDTR
560 570 580 590 600
DQYLIFGAEE GIYTLNLNEL HETSMEQLFP RRCTWLYVMN NCLLSVSGKA
610 620 630 640 650
SQLYSHNLPG LFDYARQMQK LPVAIPAHKL PDRILPRKFA VSAKIPETKW
660 670 680 690 700
CQKCCVVRNP YTGHKYLCGA LQTSIVLLEW VEPMQKFMLI KHIEFPMPCP
710 720 730 740 750
LRMFEMLVVP EQEYPLVCVG VSRGRDFNQV VRFETVNPNS TSSWFTESDT
760 770 780 790 800
PQTNVTHVTQ LERDTILVCL DCCIKIVNLQ GRLKSSRKLS SELTFDFQIE
810 820 830 840 850
SIVCLQDSVL AFWKHGMQGR SFRSNEVTQE ISDNTRIFRL LGSDRVVVLE
860 870
SRPTDNPTAN SNLYILAGHE NSY
Length:873
Mass (Da):98,679
Last modified:July 10, 2007 - v2
Checksum:i2EF27843498FA81D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AI112857 mRNA. No translation available.
AF312224 mRNA. Translation: AAK53214.1.
RefSeqiNP_596898.1. NM_133407.1.
UniGeneiRn.93345.

Genome annotation databases

GeneIDi170920.
KEGGirno:170920.
UCSCiRGD:621280. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AI112857 mRNA. No translation available.
AF312224 mRNA. Translation: AAK53214.1.
RefSeqiNP_596898.1. NM_133407.1.
UniGeneiRn.93345.

3D structure databases

ProteinModelPortaliQ924I2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi251030. 1 interaction.
MINTiMINT-199992.
STRINGi10116.ENSRNOP00000010366.

PTM databases

PhosphoSiteiQ924I2.

Proteomic databases

PaxDbiQ924I2.
PRIDEiQ924I2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi170920.
KEGGirno:170920.
UCSCiRGD:621280. rat.

Organism-specific databases

CTDi8491.
RGDi621280. Map4k3.

Phylogenomic databases

eggNOGiKOG0576. Eukaryota.
ENOG410XP9G. LUCA.
HOGENOMiHOG000230824.
HOVERGENiHBG036702.
InParanoidiQ924I2.
KOiK04406.

Miscellaneous databases

NextBioi621407.
PROiQ924I2.

Family and domain databases

InterProiIPR001180. CNH_dom.
IPR011009. Kinase-like_dom.
IPR021160. MAPKKKK.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
[Graphical view]
PfamiPF00780. CNH. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
PIRSFiPIRSF038172. MAPKKKK. 1 hit.
SMARTiSM00036. CNH. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50219. CNH. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-75.
  2. "Endophilin regulates JNK activation through its interaction with the germinal center kinase-like kinase."
    Ramjaun A.R., Angers A., Legendre-Guillemin V., Tong X.-K., McPherson P.S.
    J. Biol. Chem. 276:28913-28919(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 12-873, INTERACTION WITH SH3GL2.

Entry informationi

Entry nameiM4K3_RAT
AccessioniPrimary (citable) accession number: Q924I2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 28, 2003
Last sequence update: July 10, 2007
Last modified: May 11, 2016
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.