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Protein

WW domain-containing adapter protein with coiled-coil

Gene

Wac

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a linker between gene transcription and histone H2B monoubiquitination at 'Lys-120' (H2BK120ub1). Interacts with the RNA polymerase II transcriptional machinery via its WW domain and with RNF20-RNF40 via its coiled coil region, thereby linking and regulating H2BK120ub1 and gene transcription. Regulates the cell-cycle checkpoint activation in response to DNA damage. Positive regulator of amino acid starvation-induced autophagy. Also acts as a negative regulator of basal autophagy. Positively regulates MTOR activity by promoting, in an energy-dependent manner, the assembly of the TTT complex composed of TELO2, TTI1 and TTI2 and the RUVBL complex composed of RUVBL1 and RUVBL2 into the TTT-RUVBL complex. This leads to the dimerization of the mTORC1 complex and its subsequent activation. May negatively regulate the ubiquitin proteasome pathway.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionChromatin regulator
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-8866654. E3 ubiquitin ligases ubiquitinate target proteins.

Names & Taxonomyi

Protein namesi
Recommended name:
WW domain-containing adapter protein with coiled-coil
Gene namesi
Name:Wac
Synonyms:Kiaa1844
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:2387357. Wac.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002545591 – 646WW domain-containing adapter protein with coiled-coilAdd BLAST646

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei53PhosphoserineBy similarity1
Modified residuei131PhosphoserineBy similarity1
Modified residuei142PhosphoserineBy similarity1
Modified residuei225PhosphoserineBy similarity1
Modified residuei293PhosphothreonineBy similarity1
Modified residuei302N6-acetyllysineBy similarity1
Modified residuei446PhosphoserineBy similarity1
Modified residuei471PhosphothreonineBy similarity1
Modified residuei511PhosphoserineBy similarity1
Modified residuei523PhosphoserineBy similarity1
Modified residuei525PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated on tyrosine residues.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ924H7.
PeptideAtlasiQ924H7.
PRIDEiQ924H7.

PTM databases

iPTMnetiQ924H7.
PhosphoSitePlusiQ924H7.

Expressioni

Gene expression databases

BgeeiENSMUSG00000024283.
CleanExiMM_WAC.
ExpressionAtlasiQ924H7. baseline and differential.
GenevisibleiQ924H7. MM.

Interactioni

Subunit structurei

Interacts (via coiled coil domain) with RNF20, RNF40 and UBE2A. Interacts (via WW domain) with RNA polymerase II. Interacts with MTOR and other components of the MTOR pathway including RPTOR, RUVBL1, RUVBL2, TTI1 and TTI2.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi230359. 2 interactors.
IntActiQ924H7. 1 interactor.
MINTiMINT-4123023.
STRINGi10090.ENSMUSP00000132117.

Structurei

3D structure databases

ProteinModelPortaliQ924H7.
SMRiQ924H7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini129 – 162WWPROSITE-ProRule annotationAdd BLAST34

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili617 – 643Sequence analysisAdd BLAST27

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410ISFU. Eukaryota.
ENOG4111EBW. LUCA.
GeneTreeiENSGT00440000037780.
HOGENOMiHOG000013038.
HOVERGENiHBG057585.
InParanoidiQ924H7.
OMAiPYQTLKY.
OrthoDBiEOG091G0JAB.
PhylomeDBiQ924H7.
TreeFamiTF328635.

Family and domain databases

CDDicd00201. WW. 1 hit.
InterProiView protein in InterPro
IPR001202. WW_dom.
PfamiView protein in Pfam
PF00397. WW. 1 hit.
SMARTiView protein in SMART
SM00456. WW. 1 hit.
SUPFAMiSSF51045. SSF51045. 1 hit.
PROSITEiView protein in PROSITE
PS01159. WW_DOMAIN_1. 1 hit.
PS50020. WW_DOMAIN_2. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q924H7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVMYARKQQR LSDGCHDRRG DSQPFQALKY SSKSHPSSGD HRHEKMRDAA
60 70 80 90 100
DPSPPNKMLR RSNSPENKYS DSTGHNKAKN VHTQRVRERD GGTSYSPQEN
110 120 130 140 150
SHNHSALHSS NSHSSNPSNN PSKTSDAPYD SADDWSEHIS SSGKKYYYNC
160 170 180 190 200
RTEVSQWEKP KEWLEREQRQ KEANKLAVNS FPKDRDYRRE VMQATATSGF
210 220 230 240 250
TSGMEDKHSS DASSLLPQNI LSQTSRHNDK DYRLPRAETH SSSTPVQHPI
260 270 280 290 300
KPVVHPTATP STVPSSPFTL QSDHQPKKSF DANGASTLSK LPTPTASLPA
310 320 330 340 350
QKTERKESAP GDKSISHSCT TPSTSSASGL NPTSAPPTSA SAVPVSPVPQ
360 370 380 390 400
STIPPLLQDP NLFRQLLPAL QATLQLNNSN VDISKINEVL TAAVTQASLQ
410 420 430 440 450
SIIHKFLTAG PSAFNITSLI SQAAQLSTQA QPSNQSPMSL TSDASSPRSY
460 470 480 490 500
VSPRISTPQT NTVPMKPLIS TPPVSSQPKV STPVVKQGPV SHSATQQPVT
510 520 530 540 550
ADKQQSHDPV SPRSLQRLSS QRSPSPGPNH TCSSNASTAT VVPQNASARP
560 570 580 590 600
ACSLTPTLAA HFNDNLIKHV QGWPADHAEK QASRLREEAH NMGSVHMSEI
610 620 630 640
CTELKNLRSL VRVCEIQATL REQRILFLRQ QIKELEKLKN QNSFMV
Length:646
Mass (Da):70,680
Last modified:October 31, 2006 - v2
Checksum:i62914680DB435756
GO
Isoform 2 (identifier: Q924H7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-45: Missing.

Show »
Length:601
Mass (Da):65,455
Checksum:iAED0D65F2725EC90
GO
Isoform 3 (identifier: Q924H7-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     204-307: MEDKHSSDAS...LPAQKTERKE → K

Show »
Length:543
Mass (Da):59,491
Checksum:iF89BC56D164A0923
GO

Sequence cautioni

The sequence BAD32552 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti265S → F in BAC40017 (PubMed:16141072).Curated1
Sequence conflicti334S → P in AAK73808 (PubMed:11827461).Curated1
Sequence conflicti509P → S in AAH80851 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0212371 – 45Missing in isoform 2. 1 PublicationAdd BLAST45
Alternative sequenceiVSP_021238204 – 307MEDKH…TERKE → K in isoform 3. 1 PublicationAdd BLAST104

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF320996 mRNA. Translation: AAK73808.1.
AK173274 mRNA. Translation: BAD32552.1. Different initiation.
AK044675 mRNA. Translation: BAC32029.1.
AK087826 mRNA. Translation: BAC40017.1.
AK156780 mRNA. Translation: BAE33852.1.
BC080851 mRNA. Translation: AAH80851.1.
CCDSiCCDS29046.1. [Q924H7-1]
CCDS50218.1. [Q924H7-3]
CCDS70869.1. [Q924H7-2]
RefSeqiNP_001139770.1. NM_001146298.2. [Q924H7-3]
NP_001269022.1. NM_001282093.1. [Q924H7-2]
NP_694725.3. NM_153085.4. [Q924H7-1]
XP_006525868.1. XM_006525805.2. [Q924H7-2]
XP_011245190.1. XM_011246888.2. [Q924H7-2]
UniGeneiMm.204524.
Mm.491095.

Genome annotation databases

EnsembliENSMUST00000074919; ENSMUSP00000074454; ENSMUSG00000024283. [Q924H7-2]
ENSMUST00000092112; ENSMUSP00000089746; ENSMUSG00000024283. [Q924H7-3]
ENSMUST00000167020; ENSMUSP00000132117; ENSMUSG00000024283. [Q924H7-1]
GeneIDi225131.
KEGGimmu:225131.
UCSCiuc008eab.3. mouse. [Q924H7-1]
uc008eae.3. mouse. [Q924H7-3]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiWAC_MOUSE
AccessioniPrimary (citable) accession number: Q924H7
Secondary accession number(s): Q3U0K1
, Q66JM9, Q69Z92, Q8C2W2, Q8C8Q8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: October 31, 2006
Last modified: August 30, 2017
This is version 110 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot