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Protein

WW domain-containing adapter protein with coiled-coil

Gene

Wac

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a linker between gene transcription and histone H2B monoubiquitination at 'Lys-120' (H2BK120ub1). Interacts with the RNA polymerase II transcriptional machinery via its WW domain and with RNF20-RNF40 via its coiled coil region, thereby linking and regulating H2BK120ub1 and gene transcription. Regulates the cell-cycle checkpoint activation in response to DNA damage (By similarity). Positive regulator of amino acid starvation-induced autophagy (By similarity). May negatively regulate the ubiquitin proteasome pathway (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator

Keywords - Biological processi

Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
WW domain-containing adapter protein with coiled-coil
Gene namesi
Name:Wac
Synonyms:Kiaa1844
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:2387357. Wac.

Subcellular locationi

  • Nucleus speckle 1 Publication
  • Nucleus By similarity

  • Note: In distinct nuclear speckles. Colocalizes with pre-mRNA processing complexes.

GO - Cellular componenti

  • nuclear speck Source: UniProtKB-SubCell
  • nucleoplasm Source: MGI
  • spliceosomal complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 646646WW domain-containing adapter protein with coiled-coilPRO_0000254559Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei53 – 531PhosphoserineBy similarity
Modified residuei293 – 2931PhosphothreonineBy similarity
Modified residuei302 – 3021N6-acetyllysineBy similarity
Modified residuei446 – 4461PhosphoserineBy similarity
Modified residuei471 – 4711PhosphothreonineBy similarity
Modified residuei511 – 5111PhosphoserineBy similarity
Modified residuei523 – 5231PhosphoserineBy similarity
Modified residuei525 – 5251PhosphoserineBy similarity

Post-translational modificationi

Phosphorylated on tyrosine residues.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ924H7.
MaxQBiQ924H7.
PaxDbiQ924H7.
PRIDEiQ924H7.

PTM databases

iPTMnetiQ924H7.
PhosphoSiteiQ924H7.

Expressioni

Gene expression databases

BgeeiQ924H7.
CleanExiMM_WAC.
ExpressionAtlasiQ924H7. baseline and differential.
GenevisibleiQ924H7. MM.

Interactioni

Subunit structurei

Interacts (via coiled coil domain) with RNF20, RNF40 and UBE2A. Interacts (via WW domain) with RNA polymerase II (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi230359. 2 interactions.
IntActiQ924H7. 1 interaction.
MINTiMINT-4123023.
STRINGi10090.ENSMUSP00000132117.

Structurei

3D structure databases

ProteinModelPortaliQ924H7.
SMRiQ924H7. Positions 131-163.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini129 – 16234WWPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili617 – 64327Sequence analysisAdd
BLAST

Sequence similaritiesi

Contains 1 WW domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410ISFU. Eukaryota.
ENOG4111EBW. LUCA.
GeneTreeiENSGT00440000037780.
HOGENOMiHOG000013038.
HOVERGENiHBG057585.
InParanoidiQ924H7.
OMAiHKILTAG.
OrthoDBiEOG7T1RB9.
PhylomeDBiQ924H7.
TreeFamiTF328635.

Family and domain databases

InterProiIPR001202. WW_dom.
[Graphical view]
PfamiPF00397. WW. 1 hit.
[Graphical view]
SMARTiSM00456. WW. 1 hit.
[Graphical view]
SUPFAMiSSF51045. SSF51045. 1 hit.
PROSITEiPS01159. WW_DOMAIN_1. 1 hit.
PS50020. WW_DOMAIN_2. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q924H7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVMYARKQQR LSDGCHDRRG DSQPFQALKY SSKSHPSSGD HRHEKMRDAA
60 70 80 90 100
DPSPPNKMLR RSNSPENKYS DSTGHNKAKN VHTQRVRERD GGTSYSPQEN
110 120 130 140 150
SHNHSALHSS NSHSSNPSNN PSKTSDAPYD SADDWSEHIS SSGKKYYYNC
160 170 180 190 200
RTEVSQWEKP KEWLEREQRQ KEANKLAVNS FPKDRDYRRE VMQATATSGF
210 220 230 240 250
TSGMEDKHSS DASSLLPQNI LSQTSRHNDK DYRLPRAETH SSSTPVQHPI
260 270 280 290 300
KPVVHPTATP STVPSSPFTL QSDHQPKKSF DANGASTLSK LPTPTASLPA
310 320 330 340 350
QKTERKESAP GDKSISHSCT TPSTSSASGL NPTSAPPTSA SAVPVSPVPQ
360 370 380 390 400
STIPPLLQDP NLFRQLLPAL QATLQLNNSN VDISKINEVL TAAVTQASLQ
410 420 430 440 450
SIIHKFLTAG PSAFNITSLI SQAAQLSTQA QPSNQSPMSL TSDASSPRSY
460 470 480 490 500
VSPRISTPQT NTVPMKPLIS TPPVSSQPKV STPVVKQGPV SHSATQQPVT
510 520 530 540 550
ADKQQSHDPV SPRSLQRLSS QRSPSPGPNH TCSSNASTAT VVPQNASARP
560 570 580 590 600
ACSLTPTLAA HFNDNLIKHV QGWPADHAEK QASRLREEAH NMGSVHMSEI
610 620 630 640
CTELKNLRSL VRVCEIQATL REQRILFLRQ QIKELEKLKN QNSFMV
Length:646
Mass (Da):70,680
Last modified:October 31, 2006 - v2
Checksum:i62914680DB435756
GO
Isoform 2 (identifier: Q924H7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-45: Missing.

Show »
Length:601
Mass (Da):65,455
Checksum:iAED0D65F2725EC90
GO
Isoform 3 (identifier: Q924H7-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     204-307: MEDKHSSDAS...LPAQKTERKE → K

Show »
Length:543
Mass (Da):59,491
Checksum:iF89BC56D164A0923
GO

Sequence cautioni

The sequence BAD32552.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti265 – 2651S → F in BAC40017 (PubMed:16141072).Curated
Sequence conflicti334 – 3341S → P in AAK73808 (PubMed:11827461).Curated
Sequence conflicti509 – 5091P → S in AAH80851 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 4545Missing in isoform 2. 1 PublicationVSP_021237Add
BLAST
Alternative sequencei204 – 307104MEDKH…TERKE → K in isoform 3. 1 PublicationVSP_021238Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF320996 mRNA. Translation: AAK73808.1.
AK173274 mRNA. Translation: BAD32552.1. Different initiation.
AK044675 mRNA. Translation: BAC32029.1.
AK087826 mRNA. Translation: BAC40017.1.
AK156780 mRNA. Translation: BAE33852.1.
BC080851 mRNA. Translation: AAH80851.1.
CCDSiCCDS29046.1. [Q924H7-1]
CCDS50218.1. [Q924H7-3]
CCDS70869.1. [Q924H7-2]
RefSeqiNP_001139770.1. NM_001146298.2. [Q924H7-3]
NP_001269022.1. NM_001282093.1. [Q924H7-2]
NP_694725.3. NM_153085.4. [Q924H7-1]
XP_006525868.1. XM_006525805.2. [Q924H7-2]
XP_011245190.1. XM_011246888.1. [Q924H7-2]
UniGeneiMm.204524.
Mm.491095.

Genome annotation databases

EnsembliENSMUST00000074919; ENSMUSP00000074454; ENSMUSG00000024283. [Q924H7-2]
ENSMUST00000092112; ENSMUSP00000089746; ENSMUSG00000024283. [Q924H7-3]
ENSMUST00000167020; ENSMUSP00000132117; ENSMUSG00000024283. [Q924H7-1]
GeneIDi225131.
KEGGimmu:225131.
UCSCiuc008eab.3. mouse. [Q924H7-1]
uc008eae.3. mouse. [Q924H7-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF320996 mRNA. Translation: AAK73808.1.
AK173274 mRNA. Translation: BAD32552.1. Different initiation.
AK044675 mRNA. Translation: BAC32029.1.
AK087826 mRNA. Translation: BAC40017.1.
AK156780 mRNA. Translation: BAE33852.1.
BC080851 mRNA. Translation: AAH80851.1.
CCDSiCCDS29046.1. [Q924H7-1]
CCDS50218.1. [Q924H7-3]
CCDS70869.1. [Q924H7-2]
RefSeqiNP_001139770.1. NM_001146298.2. [Q924H7-3]
NP_001269022.1. NM_001282093.1. [Q924H7-2]
NP_694725.3. NM_153085.4. [Q924H7-1]
XP_006525868.1. XM_006525805.2. [Q924H7-2]
XP_011245190.1. XM_011246888.1. [Q924H7-2]
UniGeneiMm.204524.
Mm.491095.

3D structure databases

ProteinModelPortaliQ924H7.
SMRiQ924H7. Positions 131-163.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi230359. 2 interactions.
IntActiQ924H7. 1 interaction.
MINTiMINT-4123023.
STRINGi10090.ENSMUSP00000132117.

PTM databases

iPTMnetiQ924H7.
PhosphoSiteiQ924H7.

Proteomic databases

EPDiQ924H7.
MaxQBiQ924H7.
PaxDbiQ924H7.
PRIDEiQ924H7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000074919; ENSMUSP00000074454; ENSMUSG00000024283. [Q924H7-2]
ENSMUST00000092112; ENSMUSP00000089746; ENSMUSG00000024283. [Q924H7-3]
ENSMUST00000167020; ENSMUSP00000132117; ENSMUSG00000024283. [Q924H7-1]
GeneIDi225131.
KEGGimmu:225131.
UCSCiuc008eab.3. mouse. [Q924H7-1]
uc008eae.3. mouse. [Q924H7-3]

Organism-specific databases

CTDi51322.
MGIiMGI:2387357. Wac.
RougeiSearch...

Phylogenomic databases

eggNOGiENOG410ISFU. Eukaryota.
ENOG4111EBW. LUCA.
GeneTreeiENSGT00440000037780.
HOGENOMiHOG000013038.
HOVERGENiHBG057585.
InParanoidiQ924H7.
OMAiHKILTAG.
OrthoDBiEOG7T1RB9.
PhylomeDBiQ924H7.
TreeFamiTF328635.

Miscellaneous databases

ChiTaRSiWac. mouse.
NextBioi377540.
PROiQ924H7.
SOURCEiSearch...

Gene expression databases

BgeeiQ924H7.
CleanExiMM_WAC.
ExpressionAtlasiQ924H7. baseline and differential.
GenevisibleiQ924H7. MM.

Family and domain databases

InterProiIPR001202. WW_dom.
[Graphical view]
PfamiPF00397. WW. 1 hit.
[Graphical view]
SMARTiSM00456. WW. 1 hit.
[Graphical view]
SUPFAMiSSF51045. SSF51045. 1 hit.
PROSITEiPS01159. WW_DOMAIN_1. 1 hit.
PS50020. WW_DOMAIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "WAC, a novel WW domain-containing adapter with a coiled-coil region, is colocalized with splicing factor SC35."
    Xu G.M., Arnaout M.A.
    Genomics 79:87-94(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PHOSPHORYLATION, SUBCELLULAR LOCATION.
    Strain: C57BL/6J.
  2. "Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Seino S., Nishimura M., Kaisho T., Hoshino K., Kitamura H., Nagase T., Ohara O., Koga H.
    DNA Res. 11:205-218(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
    Tissue: Pancreatic islet.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: C57BL/6J and NOD.
    Tissue: Ovary, Retina and Spleen.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Brain.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Kidney, Lung, Pancreas and Spleen.

Entry informationi

Entry nameiWAC_MOUSE
AccessioniPrimary (citable) accession number: Q924H7
Secondary accession number(s): Q3U0K1
, Q66JM9, Q69Z92, Q8C2W2, Q8C8Q8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: October 31, 2006
Last modified: March 16, 2016
This is version 100 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.