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Protein

Neuropeptide FF receptor 2

Gene

Npffr2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Receptor for NPAF (A-18-F-amide) and NPFF (F-8-F-amide) neuropeptides, also known as morphine-modulating peptides. Can also be activated by a variety of naturally occurring or synthetic FMRF-amide like ligands. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiR-MMU-389397. Orexin and neuropeptides FF and QRFP bind to their respective receptors.
R-MMU-416476. G alpha (q) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Neuropeptide FF receptor 2
Alternative name(s):
G-protein coupled receptor 74
Neuropeptide NPFF receptor
Gene namesi
Name:Npffr2
Synonyms:Gpr74, Npff2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1860130. Npffr2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 45ExtracellularSequence analysisAdd BLAST45
Transmembranei46 – 66Helical; Name=1Sequence analysisAdd BLAST21
Topological domaini67 – 82CytoplasmicSequence analysisAdd BLAST16
Transmembranei83 – 103Helical; Name=2Sequence analysisAdd BLAST21
Topological domaini104 – 119ExtracellularSequence analysisAdd BLAST16
Transmembranei120 – 140Helical; Name=3Sequence analysisAdd BLAST21
Topological domaini141 – 160CytoplasmicSequence analysisAdd BLAST20
Transmembranei161 – 181Helical; Name=4Sequence analysisAdd BLAST21
Topological domaini182 – 217ExtracellularSequence analysisAdd BLAST36
Transmembranei218 – 238Helical; Name=5Sequence analysisAdd BLAST21
Topological domaini239 – 274CytoplasmicSequence analysisAdd BLAST36
Transmembranei275 – 295Helical; Name=6Sequence analysisAdd BLAST21
Topological domaini296 – 310ExtracellularSequence analysisAdd BLAST15
Transmembranei311 – 331Helical; Name=7Sequence analysisAdd BLAST21
Topological domaini332 – 417CytoplasmicSequence analysisAdd BLAST86

GO - Cellular componenti

  • actin cytoskeleton Source: MGI
  • integral component of plasma membrane Source: MGI
  • plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000699161 – 417Neuropeptide FF receptor 2Add BLAST417

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi8N-linked (GlcNAc...)Sequence analysis1
Glycosylationi20N-linked (GlcNAc...)Sequence analysis1
Glycosylationi31N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi118 ↔ 206PROSITE-ProRule annotation
Glycosylationi198N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ924H0.
PRIDEiQ924H0.

PTM databases

PhosphoSitePlusiQ924H0.

Expressioni

Gene expression databases

BgeeiENSMUSG00000035528.
CleanExiMM_NPFFR2.
GenevisibleiQ924H0. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000040033.

Structurei

3D structure databases

ProteinModelPortaliQ924H0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3656. Eukaryota.
ENOG410XRW9. LUCA.
GeneTreeiENSGT00760000118781.
HOGENOMiHOG000041285.
HOVERGENiHBG099624.
InParanoidiQ924H0.
KOiK08375.
OMAiNTMCKIS.
OrthoDBiEOG091G098F.
PhylomeDBiQ924H0.
TreeFamiTF315303.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR005395. NPFF_rcpt.
IPR005397. NPFF_rcpt_2.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR01570. NPFFRECEPTOR.
PR01572. NPFFRECEPTR2.
SMARTiSM01381. 7TM_GPCR_Srsx. 1 hit.
[Graphical view]
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q924H0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEKWDSNSS ESWNHIWSGN DTQHHWYSDI NITYVNYYLH QPQVAAVFIS
60 70 80 90 100
SYLLIFVLCM VGNTVVCFIV IRNRHMHTVT NFFILNLAIS DLLVGIFCMP
110 120 130 140 150
ITLLDNIIAG WPFGSSMCKI SGLVQGISVA ASVFTLVAIA VDRFRCVVYP
160 170 180 190 200
FKPKLTVKTA FVTIVIIWGL AIAIMTPSAI MLHVQEEKYY RVRLSSHNKT
210 220 230 240 250
STVYWCREDW PRHEMRRIYT TVLFATIYLA PLSLIVIMYA RIGASLFKTA
260 270 280 290 300
AHCTGKQRPV QWHVSKKKQK VIKMLLTVAL LFILSWLPLW TLMMLSDYTD
310 320 330 340 350
LSPNKLRIIN IYIYPFAHWL AFCNSSVNPI IYGFFNENFR NGFQDAFQIC
360 370 380 390 400
QKKAKPQEAY SLRAKRNIVI NTSGLLVQEP VSQNPGGENL GCGKSADNPT
410
QESLIEEMGE ATNSTVA
Length:417
Mass (Da):47,449
Last modified:May 10, 2004 - v2
Checksum:iBB8D85EF405D5786
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti89I → T in AAK94198 (Ref. 1) Curated1
Sequence conflicti260V → E in AAK94198 (Ref. 1) Curated1
Sequence conflicti368I → M in AAK94198 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF330054 mRNA. Translation: AAK94198.1.
AK050939 mRNA. Translation: BAC34468.1.
CCDSiCCDS19408.1.
RefSeqiNP_573455.2. NM_133192.3.
XP_017176078.1. XM_017320589.1.
UniGeneiMm.447881.

Genome annotation databases

EnsembliENSMUST00000048557; ENSMUSP00000040033; ENSMUSG00000035528.
GeneIDi104443.
KEGGimmu:104443.
UCSCiuc008yan.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF330054 mRNA. Translation: AAK94198.1.
AK050939 mRNA. Translation: BAC34468.1.
CCDSiCCDS19408.1.
RefSeqiNP_573455.2. NM_133192.3.
XP_017176078.1. XM_017320589.1.
UniGeneiMm.447881.

3D structure databases

ProteinModelPortaliQ924H0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000040033.

Protein family/group databases

GPCRDBiSearch...

PTM databases

PhosphoSitePlusiQ924H0.

Proteomic databases

PaxDbiQ924H0.
PRIDEiQ924H0.

Protocols and materials databases

DNASUi104443.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000048557; ENSMUSP00000040033; ENSMUSG00000035528.
GeneIDi104443.
KEGGimmu:104443.
UCSCiuc008yan.1. mouse.

Organism-specific databases

CTDi10886.
MGIiMGI:1860130. Npffr2.

Phylogenomic databases

eggNOGiKOG3656. Eukaryota.
ENOG410XRW9. LUCA.
GeneTreeiENSGT00760000118781.
HOGENOMiHOG000041285.
HOVERGENiHBG099624.
InParanoidiQ924H0.
KOiK08375.
OMAiNTMCKIS.
OrthoDBiEOG091G098F.
PhylomeDBiQ924H0.
TreeFamiTF315303.

Enzyme and pathway databases

ReactomeiR-MMU-389397. Orexin and neuropeptides FF and QRFP bind to their respective receptors.
R-MMU-416476. G alpha (q) signalling events.

Miscellaneous databases

ChiTaRSiNpffr2. mouse.
PROiQ924H0.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000035528.
CleanExiMM_NPFFR2.
GenevisibleiQ924H0. MM.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR005395. NPFF_rcpt.
IPR005397. NPFF_rcpt_2.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR01570. NPFFRECEPTOR.
PR01572. NPFFRECEPTR2.
SMARTiSM01381. 7TM_GPCR_Srsx. 1 hit.
[Graphical view]
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNPFF2_MOUSE
AccessioniPrimary (citable) accession number: Q924H0
Secondary accession number(s): Q8BKR6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: May 10, 2004
Last modified: November 2, 2016
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.