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Protein

Neuropeptide FF receptor 2

Gene

Npffr2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Receptor for NPAF (A-18-F-amide) and NPFF (F-8-F-amide) neuropeptides, also known as morphine-modulating peptides. Can also be activated by a variety of naturally occurring or synthetic FMRF-amide like ligands. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiR-MMU-389397. Orexin and neuropeptides FF and QRFP bind to their respective receptors.
R-MMU-416476. G alpha (q) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Neuropeptide FF receptor 2
Alternative name(s):
G-protein coupled receptor 74
Neuropeptide NPFF receptor
Gene namesi
Name:Npffr2
Synonyms:Gpr74, Npff2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1860130. Npffr2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 4545ExtracellularSequence analysisAdd
BLAST
Transmembranei46 – 6621Helical; Name=1Sequence analysisAdd
BLAST
Topological domaini67 – 8216CytoplasmicSequence analysisAdd
BLAST
Transmembranei83 – 10321Helical; Name=2Sequence analysisAdd
BLAST
Topological domaini104 – 11916ExtracellularSequence analysisAdd
BLAST
Transmembranei120 – 14021Helical; Name=3Sequence analysisAdd
BLAST
Topological domaini141 – 16020CytoplasmicSequence analysisAdd
BLAST
Transmembranei161 – 18121Helical; Name=4Sequence analysisAdd
BLAST
Topological domaini182 – 21736ExtracellularSequence analysisAdd
BLAST
Transmembranei218 – 23821Helical; Name=5Sequence analysisAdd
BLAST
Topological domaini239 – 27436CytoplasmicSequence analysisAdd
BLAST
Transmembranei275 – 29521Helical; Name=6Sequence analysisAdd
BLAST
Topological domaini296 – 31015ExtracellularSequence analysisAdd
BLAST
Transmembranei311 – 33121Helical; Name=7Sequence analysisAdd
BLAST
Topological domaini332 – 41786CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 417417Neuropeptide FF receptor 2PRO_0000069916Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi8 – 81N-linked (GlcNAc...)Sequence analysis
Glycosylationi20 – 201N-linked (GlcNAc...)Sequence analysis
Glycosylationi31 – 311N-linked (GlcNAc...)Sequence analysis
Disulfide bondi118 ↔ 206PROSITE-ProRule annotation
Glycosylationi198 – 1981N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ924H0.
PRIDEiQ924H0.

PTM databases

PhosphoSiteiQ924H0.

Expressioni

Gene expression databases

BgeeiQ924H0.
CleanExiMM_NPFFR2.
GenevisibleiQ924H0. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000040033.

Structurei

3D structure databases

ProteinModelPortaliQ924H0.
SMRiQ924H0. Positions 46-347.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3656. Eukaryota.
ENOG410XRW9. LUCA.
GeneTreeiENSGT00760000118781.
HOGENOMiHOG000041285.
HOVERGENiHBG099624.
InParanoidiQ924H0.
KOiK08375.
OMAiNTMCKIS.
OrthoDBiEOG7GBFXN.
PhylomeDBiQ924H0.
TreeFamiTF315303.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR005395. NPFF_rcpt.
IPR005397. NPFF_rcpt_2.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR01570. NPFFRECEPTOR.
PR01572. NPFFRECEPTR2.
SMARTiSM01381. 7TM_GPCR_Srsx. 1 hit.
[Graphical view]
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q924H0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEKWDSNSS ESWNHIWSGN DTQHHWYSDI NITYVNYYLH QPQVAAVFIS
60 70 80 90 100
SYLLIFVLCM VGNTVVCFIV IRNRHMHTVT NFFILNLAIS DLLVGIFCMP
110 120 130 140 150
ITLLDNIIAG WPFGSSMCKI SGLVQGISVA ASVFTLVAIA VDRFRCVVYP
160 170 180 190 200
FKPKLTVKTA FVTIVIIWGL AIAIMTPSAI MLHVQEEKYY RVRLSSHNKT
210 220 230 240 250
STVYWCREDW PRHEMRRIYT TVLFATIYLA PLSLIVIMYA RIGASLFKTA
260 270 280 290 300
AHCTGKQRPV QWHVSKKKQK VIKMLLTVAL LFILSWLPLW TLMMLSDYTD
310 320 330 340 350
LSPNKLRIIN IYIYPFAHWL AFCNSSVNPI IYGFFNENFR NGFQDAFQIC
360 370 380 390 400
QKKAKPQEAY SLRAKRNIVI NTSGLLVQEP VSQNPGGENL GCGKSADNPT
410
QESLIEEMGE ATNSTVA
Length:417
Mass (Da):47,449
Last modified:May 10, 2004 - v2
Checksum:iBB8D85EF405D5786
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti89 – 891I → T in AAK94198 (Ref. 1) Curated
Sequence conflicti260 – 2601V → E in AAK94198 (Ref. 1) Curated
Sequence conflicti368 – 3681I → M in AAK94198 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF330054 mRNA. Translation: AAK94198.1.
AK050939 mRNA. Translation: BAC34468.1.
CCDSiCCDS19408.1.
RefSeqiNP_573455.2. NM_133192.3.
XP_006534759.1. XM_006534696.2.
XP_011247690.1. XM_011249388.1.
UniGeneiMm.447881.

Genome annotation databases

EnsembliENSMUST00000048557; ENSMUSP00000040033; ENSMUSG00000035528.
GeneIDi104443.
KEGGimmu:104443.
UCSCiuc008yan.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF330054 mRNA. Translation: AAK94198.1.
AK050939 mRNA. Translation: BAC34468.1.
CCDSiCCDS19408.1.
RefSeqiNP_573455.2. NM_133192.3.
XP_006534759.1. XM_006534696.2.
XP_011247690.1. XM_011249388.1.
UniGeneiMm.447881.

3D structure databases

ProteinModelPortaliQ924H0.
SMRiQ924H0. Positions 46-347.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000040033.

Protein family/group databases

GPCRDBiSearch...

PTM databases

PhosphoSiteiQ924H0.

Proteomic databases

PaxDbiQ924H0.
PRIDEiQ924H0.

Protocols and materials databases

DNASUi104443.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000048557; ENSMUSP00000040033; ENSMUSG00000035528.
GeneIDi104443.
KEGGimmu:104443.
UCSCiuc008yan.1. mouse.

Organism-specific databases

CTDi10886.
MGIiMGI:1860130. Npffr2.

Phylogenomic databases

eggNOGiKOG3656. Eukaryota.
ENOG410XRW9. LUCA.
GeneTreeiENSGT00760000118781.
HOGENOMiHOG000041285.
HOVERGENiHBG099624.
InParanoidiQ924H0.
KOiK08375.
OMAiNTMCKIS.
OrthoDBiEOG7GBFXN.
PhylomeDBiQ924H0.
TreeFamiTF315303.

Enzyme and pathway databases

ReactomeiR-MMU-389397. Orexin and neuropeptides FF and QRFP bind to their respective receptors.
R-MMU-416476. G alpha (q) signalling events.

Miscellaneous databases

ChiTaRSiNpffr2. mouse.
PROiQ924H0.
SOURCEiSearch...

Gene expression databases

BgeeiQ924H0.
CleanExiMM_NPFFR2.
GenevisibleiQ924H0. MM.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR005395. NPFF_rcpt.
IPR005397. NPFF_rcpt_2.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR01570. NPFFRECEPTOR.
PR01572. NPFFRECEPTR2.
SMARTiSM01381. 7TM_GPCR_Srsx. 1 hit.
[Graphical view]
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and characterization of two cognate receptors for mammalian FMRFamide-like neuropeptides."
    Liu Q., Guan X.-M., McDonald T.P., Jiang Q., Zeng Z., Marlene J., Williams D.L. Jr., Hong Y., Figueroa D., Clements M.K., Mallee J., Wang R., Evans J., Gould R., Austin C.P.
    Submitted (DEC-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.

Entry informationi

Entry nameiNPFF2_MOUSE
AccessioniPrimary (citable) accession number: Q924H0
Secondary accession number(s): Q8BKR6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: May 10, 2004
Last modified: July 6, 2016
This is version 113 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.