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Q924C1

- XPO5_MOUSE

UniProt

Q924C1 - XPO5_MOUSE

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Protein

Exportin-5

Gene

Xpo5

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Mediates the nuclear export of proteins bearing a double-stranded RNA binding domain (dsRBD) and double-stranded RNAs (cargos). XPO5 in the nucleus binds cooperatively to the RNA and to the GTPase Ran in its active GTP-bound form. Proteins containing dsRBDs can associate with this trimeric complex through the RNA. Docking of this complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause disassembly of the complex and release of the cargo from the export receptor. XPO5 then returns to the nuclear compartment by diffusion through the nuclear pore complex, to mediate another round of transport. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Overexpression may in some circumstances enhance RNA-mediated gene silencing (RNAi) (By similarity). Mediates nuclear export of ADAR/ADAR1 in a RanGTP-dependent manner (By similarity).By similarity
Mediates the nuclear export of micro-RNA precursors, which form short hairpins. Also mediates the nuclear export of synthetic short hairpin RNAs used for RNA interference, and adenovirus VA1 dsRNA. In some circumstances can also mediate the nuclear export of deacylated and aminoacylated tRNAs. Specifically recognizes dsRNAs that lack a 5'-overhang in a sequence-independent manner, have only a short 3'-overhang, and that have a double-stranded length of at least 15 base-pairs. Binding is dependent on Ran-GTP (By similarity).By similarity

GO - Molecular functioni

  1. poly(A) RNA binding Source: Ensembl
  2. protein transporter activity Source: MGI
  3. tRNA binding Source: MGI

GO - Biological processi

  1. gene silencing by RNA Source: UniProtKB-KW
  2. protein export from nucleus Source: MGI
Complete GO annotation...

Keywords - Biological processi

Protein transport, RNA-mediated gene silencing, Transport

Keywords - Ligandi

RNA-binding, tRNA-binding

Enzyme and pathway databases

ReactomeiREACT_221178. MicroRNA (miRNA) biogenesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Exportin-5
Short name:
Exp5
Alternative name(s):
Ran-binding protein 21
Gene namesi
Name:Xpo5
Synonyms:Kiaa1291, Ranbp21
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 17

Organism-specific databases

MGIiMGI:1913789. Xpo5.

Subcellular locationi

Nucleus By similarity. Cytoplasm By similarity
Note: Shuttles between the nucleus and the cytoplasm.By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 12041203Exportin-5PRO_0000235300Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei396 – 3961N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ924C1.
PaxDbiQ924C1.
PRIDEiQ924C1.

PTM databases

PhosphoSiteiQ924C1.

Miscellaneous databases

PMAP-CutDBQ924C1.

Expressioni

Gene expression databases

BgeeiQ924C1.
CleanExiMM_XPO5.
GenevestigatoriQ924C1.

Interactioni

Subunit structurei

Component of a nuclear export receptor complex composed of XPO5, Ran, dsRNA-binding proteins and dsRNA. Found in a nuclear export complex with XPO5, Ran, EEF1A1, and aminoacylated tRNA. Found in a nuclear export complex with XPO5, Ran, ILF3 and dsRNA. Found in a nuclear export complex with XPO5, Ran and pre-miRNA. Found in a nuclear export complex with XPO5, Ran, ILF3 and minihelix VA1 dsRNA. Found in a nuclear export complex with XPO5, RAN, ILF3, ZNF346 and dsRNA. Interacts with EEF1A1, ILF3, NUP153, NUP214 and ZNF346. Interacts with Ran and cargo proteins in a GTP-dependent manner (By similarity). Interacts with ADAR/ADAR1 (via DRBM domains) (By similarity).By similarity

Protein-protein interaction databases

BioGridi215307. 2 interactions.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2 – 108107Necessary for interaction with RanBy similarityAdd
BLAST
Regioni533 – 640108Necessary for interaction with ILF3By similarityAdd
BLAST

Sequence similaritiesi

Belongs to the exportin family.Curated

Phylogenomic databases

eggNOGiNOG296759.
GeneTreeiENSGT00390000013979.
HOGENOMiHOG000236267.
HOVERGENiHBG056281.
InParanoidiQ924C1.
KOiK14289.
OMAiFADSVGH.
OrthoDBiEOG7MWGW1.
PhylomeDBiQ924C1.
TreeFamiTF323382.

Family and domain databases

Gene3Di1.25.10.10. 2 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR013598. Exportin-1/Importin-b-like.
IPR001494. Importin-beta_N.
[Graphical view]
PfamiPF08389. Xpo1. 1 hit.
[Graphical view]
SMARTiSM00913. IBN_N. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 3 hits.

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q924C1-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEMEQVNALC EELVKAVTVM MDPSSTQRYR LEALKFCEEF KEKCPICVPC
60 70 80 90 100
GLKLAEKTQI AIVRHFGLQI LEHVVKFRWN SMSRLEKVYL KNSVMELIAN
110 120 130 140 150
GTLRILEEEN HIKDVLSRIV VEMIKREWPQ HWPDMLMELD TLFRQGETQR
160 170 180 190 200
ELVMFILLRL AEDVVTFQTL PTQRRRDIQQ TLTQNMERIL NFLLNTLQEN
210 220 230 240 250
VNKYQQMKTD SSQEAEAQAN CRVSVAALNT LAGYIDWVSL NHITAENCKL
260 270 280 290 300
VETLCLLLNE QELQLGAAEC LLIAVSRKGK LEDRKRLMIL FGDVAMHYIL
310 320 330 340 350
SAAQTADGGG LVEKHYLFLK RLCQVLCALG NLLCALLALD ANIQTPINFG
360 370 380 390 400
MYLESFLAFT THPSQFLRSS THMTWGALFR HEVLSRDPAL LAVIPKYLRA
410 420 430 440 450
SMTNLVKMGF PSKTDSPSCE YSRFDFDSDE DFNAFFNSSR AQHGEVVRCV
460 470 480 490 500
CRLDPKTSFQ MAAEWLKYQL SASIDTGPVN SCSTAGTGEG GFCSIFSPSY
510 520 530 540 550
VQWEAMTFFL ESVINQMFRT LDKEELPVSD GIELLQLVLN FEIKDPLVLS
560 570 580 590 600
CVLTNVSALF PFVTYKPAFL PQVFSKLFSF VTFESVGESK APRTRAVRNV
610 620 630 640 650
RRHACSSINK MCRDYPDLVL PNFDMLYSHV KQLLSNELLL TQMEKCALME
660 670 680 690 700
ALVLVSNQFK DYERQKLFLE ELMAPVVNIW LSEEMCRALS DIDSFIAYVG
710 720 730 740 750
ADLKSCDPAV EDPCGLNRAR MSFCVYSILG VMRRTSWPSD LEEAKAGGFV
760 770 780 790 800
VGYTPSGNPI FRNPCTEQIL RLLDNLLALV RTHNTLYTPE MLTKMAEPFT
810 820 830 840 850
KALDIVESEK TAILGLPQPL LEFNDHPVYR TTLERMQRFF GILYENCYHI
860 870 880 890 900
LGKAGPSMQQ DFYTVEDLAS QLLGSAFVNL NNIPDFRLRS MLRVFVKPLV
910 920 930 940 950
LFCPSEHYET LISPILGPLF TYLHMRLSQK WHVINQRSIL CGEDEIAEDN
960 970 980 990 1000
PESQEMLEEQ LVRMLTREAM DLIMACCVSK KTADHTAAPT ADGDDEEMMA
1010 1020 1030 1040 1050
TEVAPSSVVE LTDLGKCLMK HEDVCTALLI TAFNSLTWKD TLSCQRATTQ
1060 1070 1080 1090 1100
LCWPLLKQVM SGTLLADAVT WLFTSVLKGL QMHGQHDGCM ASLVHLAFQI
1110 1120 1130 1140 1150
YEALRPRYLE IRAVMEQIPE INKESLDQFD CKLLNPSLQK AADKRRKDHF
1160 1170 1180 1190 1200
KRLIAGCIGK PLGEQFRKEV HIKNLPWLFK KPKPMLETEV LDSEEGGLAT

IFEP
Length:1,204
Mass (Da):136,973
Last modified:December 1, 2001 - v1
Checksum:i23B3C27B68F71846
GO
Isoform 2 (identifier: Q924C1-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-720: Missing.
     925-925: M → MPLSTPALVLSPQ

Show »
Length:496
Mass (Da):56,128
Checksum:i2697A0239AAE7BCE
GO
Isoform 3 (identifier: Q924C1-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     208-208: K → V
     209-1204: Missing.

Show »
Length:208
Mass (Da):24,728
Checksum:i7C32C64C229DA26C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti48 – 481V → I in AAH55455. (PubMed:15489334)Curated
Sequence conflicti143 – 1431F → S in AAH55455. (PubMed:15489334)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 720720Missing in isoform 2. 1 PublicationVSP_018462Add
BLAST
Alternative sequencei208 – 2081K → V in isoform 3. 1 PublicationVSP_018463
Alternative sequencei209 – 1204996Missing in isoform 3. 1 PublicationVSP_018464Add
BLAST
Alternative sequencei925 – 9251M → MPLSTPALVLSPQ in isoform 2. 1 PublicationVSP_018465

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF343581 mRNA. Translation: AAK68050.1.
AK122486 mRNA. Translation: BAC65768.1.
BC055455 mRNA. Translation: AAH55455.1.
BC131661 mRNA. Translation: AAI31662.1.
AK010389 mRNA. Translation: BAB26904.1.
AK011190 mRNA. Translation: BAB27455.1.
CCDSiCCDS37631.1. [Q924C1-1]
RefSeqiNP_082474.1. NM_028198.2. [Q924C1-1]
UniGeneiMm.275039.

Genome annotation databases

EnsembliENSMUST00000087031; ENSMUSP00000084257; ENSMUSG00000067150. [Q924C1-1]
GeneIDi72322.
KEGGimmu:72322.
UCSCiuc008crz.1. mouse. [Q924C1-3]
uc008csa.1. mouse. [Q924C1-1]
uc012aun.1. mouse. [Q924C1-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF343581 mRNA. Translation: AAK68050.1 .
AK122486 mRNA. Translation: BAC65768.1 .
BC055455 mRNA. Translation: AAH55455.1 .
BC131661 mRNA. Translation: AAI31662.1 .
AK010389 mRNA. Translation: BAB26904.1 .
AK011190 mRNA. Translation: BAB27455.1 .
CCDSi CCDS37631.1. [Q924C1-1 ]
RefSeqi NP_082474.1. NM_028198.2. [Q924C1-1 ]
UniGenei Mm.275039.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 215307. 2 interactions.

PTM databases

PhosphoSitei Q924C1.

Proteomic databases

MaxQBi Q924C1.
PaxDbi Q924C1.
PRIDEi Q924C1.

Protocols and materials databases

DNASUi 72322.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000087031 ; ENSMUSP00000084257 ; ENSMUSG00000067150 . [Q924C1-1 ]
GeneIDi 72322.
KEGGi mmu:72322.
UCSCi uc008crz.1. mouse. [Q924C1-3 ]
uc008csa.1. mouse. [Q924C1-1 ]
uc012aun.1. mouse. [Q924C1-2 ]

Organism-specific databases

CTDi 57510.
MGIi MGI:1913789. Xpo5.
Rougei Search...

Phylogenomic databases

eggNOGi NOG296759.
GeneTreei ENSGT00390000013979.
HOGENOMi HOG000236267.
HOVERGENi HBG056281.
InParanoidi Q924C1.
KOi K14289.
OMAi FADSVGH.
OrthoDBi EOG7MWGW1.
PhylomeDBi Q924C1.
TreeFami TF323382.

Enzyme and pathway databases

Reactomei REACT_221178. MicroRNA (miRNA) biogenesis.

Miscellaneous databases

ChiTaRSi Xpo5. mouse.
NextBioi 336012.
PMAP-CutDB Q924C1.
PROi Q924C1.
SOURCEi Search...

Gene expression databases

Bgeei Q924C1.
CleanExi MM_XPO5.
Genevestigatori Q924C1.

Family and domain databases

Gene3Di 1.25.10.10. 2 hits.
InterProi IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR013598. Exportin-1/Importin-b-like.
IPR001494. Importin-beta_N.
[Graphical view ]
Pfami PF08389. Xpo1. 1 hit.
[Graphical view ]
SMARTi SM00913. IBN_N. 1 hit.
[Graphical view ]
SUPFAMi SSF48371. SSF48371. 3 hits.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Exp5 exports eEF1A via tRNA from nuclei and synergizes with other transport pathways to confine translation to the cytoplasm."
    Bohnsack M.T., Regener K., Schwappach B., Saffrich R., Paraskeva E., Hartmann E., Goerlich D.
    EMBO J. 21:6205-6215(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. "Prediction of the coding sequences of mouse homologues of KIAA gene: II. The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Aizawa H., Yuasa S., Nakajima D., Nagase T., Ohara O., Koga H.
    DNA Res. 10:35-48(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Brain.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
    Strain: FVB/N.
    Tissue: Mammary gland.
  4. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-521 AND 959-1204.
    Strain: C57BL/6J.

Entry informationi

Entry nameiXPO5_MOUSE
AccessioniPrimary (citable) accession number: Q924C1
Secondary accession number(s): A2RRJ5
, Q7TMP2, Q80TF9, Q9CRI9, Q9CT48
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 16, 2006
Last sequence update: December 1, 2001
Last modified: November 26, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3