Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q924C1

- XPO5_MOUSE

UniProt

Q924C1 - XPO5_MOUSE

Protein

Exportin-5

Gene

Xpo5

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 84 (01 Oct 2014)
      Sequence version 1 (01 Dec 2001)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Mediates the nuclear export of proteins bearing a double-stranded RNA binding domain (dsRBD) and double-stranded RNAs (cargos). XPO5 in the nucleus binds cooperatively to the RNA and to the GTPase Ran in its active GTP-bound form. Proteins containing dsRBDs can associate with this trimeric complex through the RNA. Docking of this complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause disassembly of the complex and release of the cargo from the export receptor. XPO5 then returns to the nuclear compartment by diffusion through the nuclear pore complex, to mediate another round of transport. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Overexpression may in some circumstances enhance RNA-mediated gene silencing (RNAi) By similarity. Mediates nuclear export of ADAR/ADAR1 in a RanGTP-dependent manner By similarity.By similarity
    Mediates the nuclear export of micro-RNA precursors, which form short hairpins. Also mediates the nuclear export of synthetic short hairpin RNAs used for RNA interference, and adenovirus VA1 dsRNA. In some circumstances can also mediate the nuclear export of deacylated and aminoacylated tRNAs. Specifically recognizes dsRNAs that lack a 5'-overhang in a sequence-independent manner, have only a short 3'-overhang, and that have a double-stranded length of at least 15 base-pairs. Binding is dependent on Ran-GTP By similarity.By similarity

    GO - Molecular functioni

    1. protein transporter activity Source: MGI
    2. tRNA binding Source: MGI

    GO - Biological processi

    1. gene silencing by RNA Source: UniProtKB-KW
    2. protein export from nucleus Source: MGI

    Keywords - Biological processi

    Protein transport, RNA-mediated gene silencing, Transport

    Keywords - Ligandi

    RNA-binding, tRNA-binding

    Enzyme and pathway databases

    ReactomeiREACT_221178. MicroRNA (miRNA) biogenesis.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Exportin-5
    Short name:
    Exp5
    Alternative name(s):
    Ran-binding protein 21
    Gene namesi
    Name:Xpo5
    Synonyms:Kiaa1291, Ranbp21
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 17

    Organism-specific databases

    MGIiMGI:1913789. Xpo5.

    Subcellular locationi

    Nucleus By similarity. Cytoplasm By similarity
    Note: Shuttles between the nucleus and the cytoplasm.By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 12041203Exportin-5PRO_0000235300Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei396 – 3961N6-acetyllysineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiQ924C1.
    PaxDbiQ924C1.
    PRIDEiQ924C1.

    PTM databases

    PhosphoSiteiQ924C1.

    Miscellaneous databases

    PMAP-CutDBQ924C1.

    Expressioni

    Gene expression databases

    BgeeiQ924C1.
    CleanExiMM_XPO5.
    GenevestigatoriQ924C1.

    Interactioni

    Subunit structurei

    Component of a nuclear export receptor complex composed of XPO5, Ran, dsRNA-binding proteins and dsRNA. Found in a nuclear export complex with XPO5, Ran, EEF1A1, and aminoacylated tRNA. Found in a nuclear export complex with XPO5, Ran, ILF3 and dsRNA. Found in a nuclear export complex with XPO5, Ran and pre-miRNA. Found in a nuclear export complex with XPO5, Ran, ILF3 and minihelix VA1 dsRNA. Found in a nuclear export complex with XPO5, RAN, ILF3, ZNF346 and dsRNA. Interacts with EEF1A1, ILF3, NUP153, NUP214 and ZNF346. Interacts with Ran and cargo proteins in a GTP-dependent manner By similarity. Interacts with ADAR/ADAR1 (via DRBM domains) By similarity.By similarity

    Protein-protein interaction databases

    BioGridi215307. 2 interactions.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni2 – 108107Necessary for interaction with RanBy similarityAdd
    BLAST
    Regioni533 – 640108Necessary for interaction with ILF3By similarityAdd
    BLAST

    Sequence similaritiesi

    Belongs to the exportin family.Curated

    Phylogenomic databases

    eggNOGiNOG296759.
    GeneTreeiENSGT00390000013979.
    HOGENOMiHOG000236267.
    HOVERGENiHBG056281.
    InParanoidiA2RRJ5.
    KOiK14289.
    OMAiFADSVGH.
    OrthoDBiEOG7MWGW1.
    PhylomeDBiQ924C1.
    TreeFamiTF323382.

    Family and domain databases

    Gene3Di1.25.10.10. 2 hits.
    InterProiIPR011989. ARM-like.
    IPR016024. ARM-type_fold.
    IPR013598. Exportin-1/Importin-b-like.
    IPR001494. Importin-beta_N.
    [Graphical view]
    PfamiPF08389. Xpo1. 1 hit.
    [Graphical view]
    SMARTiSM00913. IBN_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF48371. SSF48371. 3 hits.

    Sequences (3)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q924C1-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MEMEQVNALC EELVKAVTVM MDPSSTQRYR LEALKFCEEF KEKCPICVPC     50
    GLKLAEKTQI AIVRHFGLQI LEHVVKFRWN SMSRLEKVYL KNSVMELIAN 100
    GTLRILEEEN HIKDVLSRIV VEMIKREWPQ HWPDMLMELD TLFRQGETQR 150
    ELVMFILLRL AEDVVTFQTL PTQRRRDIQQ TLTQNMERIL NFLLNTLQEN 200
    VNKYQQMKTD SSQEAEAQAN CRVSVAALNT LAGYIDWVSL NHITAENCKL 250
    VETLCLLLNE QELQLGAAEC LLIAVSRKGK LEDRKRLMIL FGDVAMHYIL 300
    SAAQTADGGG LVEKHYLFLK RLCQVLCALG NLLCALLALD ANIQTPINFG 350
    MYLESFLAFT THPSQFLRSS THMTWGALFR HEVLSRDPAL LAVIPKYLRA 400
    SMTNLVKMGF PSKTDSPSCE YSRFDFDSDE DFNAFFNSSR AQHGEVVRCV 450
    CRLDPKTSFQ MAAEWLKYQL SASIDTGPVN SCSTAGTGEG GFCSIFSPSY 500
    VQWEAMTFFL ESVINQMFRT LDKEELPVSD GIELLQLVLN FEIKDPLVLS 550
    CVLTNVSALF PFVTYKPAFL PQVFSKLFSF VTFESVGESK APRTRAVRNV 600
    RRHACSSINK MCRDYPDLVL PNFDMLYSHV KQLLSNELLL TQMEKCALME 650
    ALVLVSNQFK DYERQKLFLE ELMAPVVNIW LSEEMCRALS DIDSFIAYVG 700
    ADLKSCDPAV EDPCGLNRAR MSFCVYSILG VMRRTSWPSD LEEAKAGGFV 750
    VGYTPSGNPI FRNPCTEQIL RLLDNLLALV RTHNTLYTPE MLTKMAEPFT 800
    KALDIVESEK TAILGLPQPL LEFNDHPVYR TTLERMQRFF GILYENCYHI 850
    LGKAGPSMQQ DFYTVEDLAS QLLGSAFVNL NNIPDFRLRS MLRVFVKPLV 900
    LFCPSEHYET LISPILGPLF TYLHMRLSQK WHVINQRSIL CGEDEIAEDN 950
    PESQEMLEEQ LVRMLTREAM DLIMACCVSK KTADHTAAPT ADGDDEEMMA 1000
    TEVAPSSVVE LTDLGKCLMK HEDVCTALLI TAFNSLTWKD TLSCQRATTQ 1050
    LCWPLLKQVM SGTLLADAVT WLFTSVLKGL QMHGQHDGCM ASLVHLAFQI 1100
    YEALRPRYLE IRAVMEQIPE INKESLDQFD CKLLNPSLQK AADKRRKDHF 1150
    KRLIAGCIGK PLGEQFRKEV HIKNLPWLFK KPKPMLETEV LDSEEGGLAT 1200
    IFEP 1204
    Length:1,204
    Mass (Da):136,973
    Last modified:December 1, 2001 - v1
    Checksum:i23B3C27B68F71846
    GO
    Isoform 2 (identifier: Q924C1-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         2-720: Missing.
         925-925: M → MPLSTPALVLSPQ

    Show »
    Length:497
    Mass (Da):56,259
    Checksum:iF33B026ABE3C32EA
    GO
    Isoform 3 (identifier: Q924C1-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         208-208: K → V
         209-1204: Missing.

    Show »
    Length:208
    Mass (Da):24,728
    Checksum:i7C32C64C229DA26C
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti48 – 481V → I in AAH55455. (PubMed:15489334)Curated
    Sequence conflicti143 – 1431F → S in AAH55455. (PubMed:15489334)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei2 – 720719Missing in isoform 2. 1 PublicationVSP_018462Add
    BLAST
    Alternative sequencei208 – 2081K → V in isoform 3. 1 PublicationVSP_018463
    Alternative sequencei209 – 1204996Missing in isoform 3. 1 PublicationVSP_018464Add
    BLAST
    Alternative sequencei925 – 9251M → MPLSTPALVLSPQ in isoform 2. 1 PublicationVSP_018465

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF343581 mRNA. Translation: AAK68050.1.
    AK122486 mRNA. Translation: BAC65768.1.
    BC055455 mRNA. Translation: AAH55455.1.
    BC131661 mRNA. Translation: AAI31662.1.
    AK010389 mRNA. Translation: BAB26904.1.
    AK011190 mRNA. Translation: BAB27455.1.
    CCDSiCCDS37631.1. [Q924C1-1]
    RefSeqiNP_082474.1. NM_028198.2. [Q924C1-1]
    UniGeneiMm.275039.

    Genome annotation databases

    EnsembliENSMUST00000087031; ENSMUSP00000084257; ENSMUSG00000067150. [Q924C1-1]
    GeneIDi72322.
    KEGGimmu:72322.
    UCSCiuc008crz.1. mouse. [Q924C1-3]
    uc008csa.1. mouse. [Q924C1-1]
    uc012aun.1. mouse. [Q924C1-2]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF343581 mRNA. Translation: AAK68050.1 .
    AK122486 mRNA. Translation: BAC65768.1 .
    BC055455 mRNA. Translation: AAH55455.1 .
    BC131661 mRNA. Translation: AAI31662.1 .
    AK010389 mRNA. Translation: BAB26904.1 .
    AK011190 mRNA. Translation: BAB27455.1 .
    CCDSi CCDS37631.1. [Q924C1-1 ]
    RefSeqi NP_082474.1. NM_028198.2. [Q924C1-1 ]
    UniGenei Mm.275039.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 215307. 2 interactions.

    PTM databases

    PhosphoSitei Q924C1.

    Proteomic databases

    MaxQBi Q924C1.
    PaxDbi Q924C1.
    PRIDEi Q924C1.

    Protocols and materials databases

    DNASUi 72322.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000087031 ; ENSMUSP00000084257 ; ENSMUSG00000067150 . [Q924C1-1 ]
    GeneIDi 72322.
    KEGGi mmu:72322.
    UCSCi uc008crz.1. mouse. [Q924C1-3 ]
    uc008csa.1. mouse. [Q924C1-1 ]
    uc012aun.1. mouse. [Q924C1-2 ]

    Organism-specific databases

    CTDi 57510.
    MGIi MGI:1913789. Xpo5.
    Rougei Search...

    Phylogenomic databases

    eggNOGi NOG296759.
    GeneTreei ENSGT00390000013979.
    HOGENOMi HOG000236267.
    HOVERGENi HBG056281.
    InParanoidi A2RRJ5.
    KOi K14289.
    OMAi FADSVGH.
    OrthoDBi EOG7MWGW1.
    PhylomeDBi Q924C1.
    TreeFami TF323382.

    Enzyme and pathway databases

    Reactomei REACT_221178. MicroRNA (miRNA) biogenesis.

    Miscellaneous databases

    ChiTaRSi XPO5. mouse.
    NextBioi 336012.
    PMAP-CutDB Q924C1.
    PROi Q924C1.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q924C1.
    CleanExi MM_XPO5.
    Genevestigatori Q924C1.

    Family and domain databases

    Gene3Di 1.25.10.10. 2 hits.
    InterProi IPR011989. ARM-like.
    IPR016024. ARM-type_fold.
    IPR013598. Exportin-1/Importin-b-like.
    IPR001494. Importin-beta_N.
    [Graphical view ]
    Pfami PF08389. Xpo1. 1 hit.
    [Graphical view ]
    SMARTi SM00913. IBN_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48371. SSF48371. 3 hits.
    ProtoNeti Search...

    Publicationsi

    1. "Exp5 exports eEF1A via tRNA from nuclei and synergizes with other transport pathways to confine translation to the cytoplasm."
      Bohnsack M.T., Regener K., Schwappach B., Saffrich R., Paraskeva E., Hartmann E., Goerlich D.
      EMBO J. 21:6205-6215(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    2. "Prediction of the coding sequences of mouse homologues of KIAA gene: II. The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
      Okazaki N., Kikuno R., Ohara R., Inamoto S., Aizawa H., Yuasa S., Nakajima D., Nagase T., Ohara O., Koga H.
      DNA Res. 10:35-48(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Brain.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
      Strain: FVB/N.
      Tissue: Mammary gland.
    4. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-521 AND 959-1204.
      Strain: C57BL/6J.

    Entry informationi

    Entry nameiXPO5_MOUSE
    AccessioniPrimary (citable) accession number: Q924C1
    Secondary accession number(s): A2RRJ5
    , Q7TMP2, Q80TF9, Q9CRI9, Q9CT48
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 16, 2006
    Last sequence update: December 1, 2001
    Last modified: October 1, 2014
    This is version 84 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3