Reviewed, UniProtKB/Swiss-Prot Q92499 (DDX1_HUMAN)

Names and origin

Protein names

Recommended name:
    ATP-dependent RNA helicase DDX1
    EC=3.6.1.-
Alternative name(s):
    DEAD box protein 1
    DEAD box protein retinoblastoma
      Short name=DBP-RB

Gene names

Name:

DDX1

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	740 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Tissue specificity	Highest levels of transcription in 2 retinoblastoma cell lines and in tissues of neuroectodermal origin including the retina, brain, and spinal cord. Ref.1
Sequence similarities	Belongs to the DEAD box helicase family. DDX1 subfamily. Contains 1 B30.2/SPRY domain. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding RNA-binding
Molecular function	Helicase Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	multicellular organismal development Ref.1 Inferred from expression pattern. Source: UniProtKB regulation of translational initiation Ref.1 Non-traceable author statement. Source: UniProtKB spliceosome assembly Ref.1 Non-traceable author statement. Source: UniProtKB
Cellular component	ribonucleoprotein complex Inferred from direct assay. Source: MGI
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent helicase activity Non-traceable author statement. Source: UniProtKB RNA binding Inferred from electronic annotation. Source: UniProtKB-KW RNA helicase activity Ref.1 Traceable author statement. Source: ProtInc protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
NDRG1	Q92597	1	EBI-358474,EBI-716486

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 740	740	ATP-dependent RNA helicase DDX1		PRO_0000054986
Regions
	Domain	2 – 428	427	Helicase ATP-binding
	Domain	70 – 247	178	B30.2/SPRY
	Domain	493 – 681	189	Helicase C-terminal
	Nucleotide binding	46 – 53	8	ATP By similarity
	Motif	370 – 373	4	DEAD box
Amino acid modifications
	Modified residue	80	1	Phosphothreonine Ref.4
	Modified residue	83	1	Phosphothreonine Ref.4
	Modified residue	628	1	Phosphotyrosine Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q92499-1 [UniParc]. Last modified August 1, 1998. Version 2. Checksum: C6D0179F83BD8C73	FASTA	740	82,432
	10 20 30 40 50 60 MAAFSEMGVM PEIAQAVEEM DWLLPTDIQA ESIPLILGGG DVLMAAETGS GKTGAFSIPV 70 80 90 100 110 120 IQIVYETLKD QQEGKKGKTT IKTGASVLNK WQMNPYDRGS AFAIGSDGLC CQSREVKEWH 130 140 150 160 170 180 GCRATKGLMK GKHYYEVSCH DQGLCRVGWS TMQASLDLGT DKFGFGFGGT GKKSHNKQFD 190 200 210 220 230 240 NYGEEFTMHD TIGCYLDIDK GHVKFSKNGK DLGLAFEIPP HMKNQALFPA CVLKNAELKF 250 260 270 280 290 300 NFGEEEFKFP PKDGFVALSK APDGYIVKSQ HSGNAQVTQT KFLPNAPKAL IVEPSRELAE 310 320 330 340 350 360 QTLNNIKQFK KYIDNPKLRE LLIIGGVAAR DQLSVLENGV DIVVGTPGRL DDLVSTGKLN 370 380 390 400 410 420 LSQVRFLVLD EADGLLSQGY SDFINRMHNQ IPQVTSDGKR LQVIVCSATL HSFDVKKLSE 430 440 450 460 470 480 KIMHFPTWVD LKGEDSVPDT VHHVVVPVNP KTDRLWERLG KSHIRTDDVH AKDNTRPGAN 490 500 510 520 530 540 SPEMWSEAIK ILKGEYAVRA IKEHKMDQAI IFCRTKIDCD NLEQYFIQQG GGPDKKGHQF 550 560 570 580 590 600 SCVCLHGDRK PHERKQNLER FKKGDVRFLI CTDVAARGID IHGVPYVINV TLPDEKQNYV 610 620 630 640 650 660 HRIGRVGRAE RMGLAISLVA TEKEKVWYHV CSSRGKGCYN TRLKEDGGCT IWYNEMQLLS 670 680 690 700 710 720 EIEEHLNCTI SQVEPDIKVP VDEFDGKVTY GQKRAAGGGS YKGHVDILAP TVQELAALEK 730 740 EAQTSFLHLG YLPNQLFRTF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Amplification of a DEAD box protein gene in retinoblastoma cell lines." Godbout R., Squire J. Proc. Natl. Acad. Sci. U.S.A. 90:7578-7582(1993) [PubMed: 7689221] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Tissue: Retinoblastoma.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung and Uterus.
[3]	"Global survey of phosphotyrosine signaling identifies oncogenic kinases in lung cancer." Rikova K., Guo A., Zeng Q., Possemato A., Yu J., Haack H., Nardone J., Lee K., Reeves C., Li Y., Hu Y., Tan Z., Stokes M., Sullivan L., Mitchell J., Wetzel R., Macneill J., Ren J.M. , Yuan J., Bakalarski C.E., Villen J., Kornhauser J.M., Smith B., Li D., Zhou X., Gygi S.P., Gu T.-L., Polakiewicz R.D., Rush J., Comb M.J. Cell 131:1190-1203(2007) [PubMed: 18083107] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-628, MASS SPECTROMETRY.
[4]	"Global proteomic profiling of phosphopeptides using electron transfer dissociation tandem mass spectrometry." Molina H., Horn D.M., Tang N., Mathivanan S., Pandey A. Proc. Natl. Acad. Sci. U.S.A. 104:2199-2204(2007) [PubMed: 17287340] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-80 AND THR-83, MASS SPECTROMETRY.
[5]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X70649 mRNA. Translation: CAA49992.1. BC012132 mRNA. Translation: AAH12132.1. BC053673 mRNA. Translation: AAH53673.1.
IPI	IPI00293655.
RefSeq	NP_004930.1.
UniGene	Hs.440599
3D structure databases
HSSP	HSSP built from PDB template 1FUK based on UniProtKB P10081.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q92499. 5 interactions.
PTM databases
PhosphoSite	Q92499.
2-D gel databases
REPRODUCTION-2DPAGE	IPI00293655.
Proteomic databases
PeptideAtlas	Q92499.
PRIDE	Q92499.
Genome annotation databases
Ensembl	ENSG00000079785. Homo sapiens. [Contig view]
GeneID	1653.
KEGG	hsa:1653.
UCSC	uc002rce.1. human.
Organism-specific databases
GeneCards	GC02P015682.
H-InvDB	HIX0001841.
HGNC	HGNC:2734. DDX1.
HPA	CAB012280.
MIM	601257. gene.
PharmGKB	PA27199.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q92499.
HOVERGEN	Q92499.
OMA	Q92499. IRTDEVH.
Gene expression databases
ArrayExpress	Q92499.
Bgee	Q92499.
CleanEx	HS_DDX1.
GermOnline	ENSG00000079785. Homo sapiens.
Family and domain databases
InterPro	IPR001870. B302. IPR014001. DEAD-like_N. IPR001650. DNA/RNA_helicase_C. IPR011545. DNA/RNA_helicase_DEAD/DEAH_N. IPR014021. Helicase_SF1/SF2_ATP-bd. IPR000629. RNA-helicase_DEAD-box_CS. IPR014014. RNA_helicase_DEAD_Q_motif. IPR018355. SPla/RYanodine_receptor_sg. IPR003877. SPRY_rcpt. [Graphical view]
Pfam	PF00270. DEAD. 1 hit. PF00271. Helicase_C. 1 hit. PF00622. SPRY. 1 hit. [Graphical view]
SMART	SM00487. DEXDc. 1 hit. SM00490. HELICc. 1 hit. SM00449. SPRY. 1 hit. [Graphical view]
PROSITE	PS50188. B302_SPRY. 1 hit. PS00039. DEAD_ATP_HELICASE. False negative. PS51192. HELICASE_ATP_BIND_1. 2 hits. PS51194. HELICASE_CTER. 1 hit. PS51195. Q_MOTIF. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	6806.
SOURCE	Search...

Entry information

Entry name

DDX1_HUMAN

Accession

Primary (citable) accession number: Q92499

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	August 1, 1998
Last modified:	July 7, 2009
This is version 85 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)