Reviewed, UniProtKB/Swiss-Prot Q92459 (FDFT_USTMA)

Names and origin

Protein names

Recommended name:
    Squalene synthetase
      Short name=SQS
      Short name=SS
    EC=2.5.1.21
Alternative name(s):
    Farnesyl-diphosphate farnesyltransferase
    FPP:FPP farnesyltransferase

Gene names

Name:	ERG9
ORF Names:	UM04374

Organism

Ustilago maydis (Smut fungus) [Complete proteome]

Taxonomic identifier

5270 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Basidiomycota › Ustilaginomycotina › Ustilaginomycetes › Ustilaginales › Ustilaginaceae › Ustilago

Protein attributes

Sequence length	572 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2 farnesyl diphosphate = diphosphate + presqualene diphosphate. Presqualene diphosphate + NAD(P)H = squalene + diphosphate + NAD(P)+.
Cofactor	Magnesium.
Pathway	Terpene metabolism; lanosterol biosynthesis; lanosterol from farnesyl diphosphate: step 1/3.
Subunit structure	Monomer By similarity.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the phytoene/squalene synthetase family.

Ontologies

Keywords
Biological process	Cholesterol biosynthesis Isoprene biosynthesis Lipid synthesis Steroid biosynthesis Sterol biosynthesis
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane
Ligand	Magnesium NADP
Molecular function	Oxidoreductase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	cholesterol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW isoprenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	farnesyl-diphosphate farnesyltransferase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW oxidoreductase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 572	572	Squalene synthetase		PRO_0000067452
Regions
	Transmembrane	316 – 336	21	Potential
	Transmembrane	492 – 512	21	Potential
Experimental info
	Sequence conflict	71 – 72	2	EL → DV in CAA68054. Ref.1
	Sequence conflict	428	1	S → T in CAA68054. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q92459-1 [UniParc]. Last modified December 12, 2006. Version 2. Checksum: 26FA62E2CA677C10	FASTA	572	65,523
	10 20 30 40 50 60 MGLLSYILLG FTHPSELRAM IGYKVWRDPL NDIKANPQAS GWDRQRMRDC WGFLDLTSRS 70 80 90 100 110 120 FAAVIKELKG ELSRVICLFY LVLRALDTVE DDMTIPAQRK IPLLVNFYKY LEQPGWNFTE 130 140 150 160 170 180 SGPNEKDRQL LVEFDKVIAE YQLLDVGYKT VISDITAKMG AGMASYIELS AKGPLKVAMW 190 200 210 220 230 240 KHFDLYCHFV AGLVGEGLSR LFSESKLERP WLGHQLELSN HMGLFLQKTN IIRDYAEDCE 250 260 270 280 290 300 EGRYFWPQQC WGDDFAKFES QPDVAKGIIE IKPGHFRPAD NELGQRSMYV LSSMLLDAMS 310 320 330 340 350 360 HATHALDYLA LLKEQSVFNF CAIPQVMAIA TLELMFNNPD VFKKNVKIRK GVAVGLILRA 370 380 390 400 410 420 VNPRDVAYTF LHYSRKMHAR LSPADPNFTR WSVELARIEQ WCETYYPSFI AAASEGKPTD 430 440 450 460 470 480 IRANALRSWS ESRRTQALIL KQAKLNGSDA STLDAKSVLE AAQNSALDPR DLMTEDERAA 490 500 510 520 530 540 QDKRDRDQMV KFFLIILVGM VTFMGIVALI TWEIVWWWTM DTPDPLSVYV KHAYYLVKTQ 550 560 570 GWSTVKEVLR TTRLSFEHVW KHGLTSPPKL EL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Squalene synthase in plant pathogenic fungi." Corran A.J. Submitted (SEP-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: IMI 103761.
[2]	"Insights from the genome of the biotrophic fungal plant pathogen Ustilago maydis." Kaemper J., Kahmann R., Boelker M., Ma L.-J., Brefort T., Saville B.J., Banuett F., Kronstad J.W., Gold S.E., Mueller O., Perlin M.H., Woesten H.A.B., de Vries R., Ruiz-Herrera J., Reynaga-Pena C.G., Snetselaar K., McCann M., Perez-Martin J. , Feldbruegge M., Basse C.W., Steinberg G., Ibeas J.I., Holloman W., Guzman P., Farman M.L., Stajich J.E., Sentandreu R., Gonzalez-Prieto J.M., Kennell J.C., Molina L., Schirawski J., Mendoza-Mendoza A., Greilinger D., Muench K., Roessel N., Scherer M., Vranes M., Ladendorf O., Vincon V., Fuchs U., Sandrock B., Meng S., Ho E.C.H., Cahill M.J., Boyce K.J., Klose J., Klosterman S.J., Deelstra H.J., Ortiz-Castellanos L., Li W., Sanchez-Alonso P., Schreier P.H., Haeuser-Hahn I., Vaupel M., Koopmann E., Friedrich G., Voss H., Schlueter T., Margolis J., Platt D., Swimmer C., Gnirke A., Chen F., Vysotskaia V., Mannhaupt G., Gueldener U., Muensterkoetter M., Haase D., Oesterheld M., Mewes H.-W., Mauceli E.W., DeCaprio D., Wade C.M., Butler J., Young S.K., Jaffe D.B., Calvo S.E., Nusbaum C., Galagan J.E., Birren B.W. Nature 444:97-101(2006) [PubMed: 17080091] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 521.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X99718 Genomic DNA. Translation: CAA68054.1. AACP01000154 Genomic DNA. Translation: EAK85649.1.
RefSeq	XP_760521.1.
3D structure databases
HSSP	HSSP built from PDB template 1EZF based on UniProtKB P37268.
ModBase	Search...
Protein-protein interaction databases
STRING	Q92459.
Genome annotation databases
GeneID	3632482.
KEGG	uma:UM04374.1.
Phylogenomic databases
OMA	MSHATHA.
Enzyme and pathway databases
BRENDA	2.5.1.21. 2320.
Family and domain databases
InterPro	IPR002060. Squ/phyt_synthse. IPR006449. Squal_synth. IPR019845. Squalene/phytoene_synthase_CS. IPR008949. Terpenoid_synth. [Graphical view]
Gene3D	G3DSA:1.10.600.10. Terpenoid_synth. 1 hit.
Pfam	PF00494. SQS_PSY. 1 hit. [Graphical view]
TIGRFAMs	TIGR01559. squal_synth. 1 hit.
PROSITE	PS01044. SQUALEN_PHYTOEN_SYN_1. 1 hit. PS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FDFT_USTMA

Accession

Primary (citable) accession number: Q92459
Secondary accession number(s): Q4P689

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	December 12, 2006
Last modified:	November 3, 2009
This is version 64 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)