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Protein

Squalene synthase

Gene

ERG9

Organism
Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the condensation of 2 two farnesyl pyrophosphate moieties to form squalene. It is the first committed enzyme of the sterol biosynthesis pathway. Required for the biosynthesis of ergosterol (By similarity).By similarity

Catalytic activityi

2 farnesyl diphosphate + NAD(P)H = squalene + 2 diphosphate + NAD(P)+.

Cofactori

Mg2+By similarity

Pathwayi

GO - Molecular functioni

  1. farnesyl-diphosphate farnesyltransferase activity Source: UniProtKB-EC
  2. oxidoreductase activity Source: UniProtKB-KW
  3. squalene synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. isoprenoid biosynthetic process Source: UniProtKB-KW
  2. sterol biosynthetic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase, Transferase

Keywords - Biological processi

Isoprene biosynthesis, Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

Keywords - Ligandi

Magnesium, NADP

Enzyme and pathway databases

UniPathwayiUPA00767; UER00751.

Names & Taxonomyi

Protein namesi
Recommended name:
Squalene synthase (EC:2.5.1.21)
Short name:
SQS
Short name:
SS
Alternative name(s):
FPP:FPP farnesyltransferase
Farnesyl-diphosphate farnesyltransferase
Gene namesi
Name:ERG9
ORF Names:UM04374
OrganismiUstilago maydis (strain 521 / FGSC 9021) (Corn smut fungus)
Taxonomic identifieri237631 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaUstilaginomycotinaUstilaginomycetesUstilaginalesUstilaginaceaeUstilago
ProteomesiUP000000561: Unassembled WGS sequence

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei316 – 33621HelicalSequence AnalysisAdd
BLAST
Transmembranei492 – 51221HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 572572Squalene synthasePRO_0000067452Add
BLAST

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi5270.UM04374.1.

Structurei

3D structure databases

ProteinModelPortaliQ92459.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the phytoene/squalene synthase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1562.
HOGENOMiHOG000186940.
InParanoidiQ92459.
KOiK00801.
OMAiTFTGNGP.
OrthoDBiEOG7GJ6P5.

Family and domain databases

Gene3Di1.10.600.10. 1 hit.
InterProiIPR002060. Squ/phyt_synthse.
IPR006449. Squal_synth.
IPR019845. Squalene/phytoene_synthase_CS.
IPR008949. Terpenoid_synth.
[Graphical view]
PfamiPF00494. SQS_PSY. 1 hit.
[Graphical view]
SUPFAMiSSF48576. SSF48576. 1 hit.
TIGRFAMsiTIGR01559. squal_synth. 1 hit.
PROSITEiPS01044. SQUALEN_PHYTOEN_SYN_1. 1 hit.
PS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q92459-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGLLSYILLG FTHPSELRAM IGYKVWRDPL NDIKANPQAS GWDRQRMRDC
60 70 80 90 100
WGFLDLTSRS FAAVIKELKG ELSRVICLFY LVLRALDTVE DDMTIPAQRK
110 120 130 140 150
IPLLVNFYKY LEQPGWNFTE SGPNEKDRQL LVEFDKVIAE YQLLDVGYKT
160 170 180 190 200
VISDITAKMG AGMASYIELS AKGPLKVAMW KHFDLYCHFV AGLVGEGLSR
210 220 230 240 250
LFSESKLERP WLGHQLELSN HMGLFLQKTN IIRDYAEDCE EGRYFWPQQC
260 270 280 290 300
WGDDFAKFES QPDVAKGIIE IKPGHFRPAD NELGQRSMYV LSSMLLDAMS
310 320 330 340 350
HATHALDYLA LLKEQSVFNF CAIPQVMAIA TLELMFNNPD VFKKNVKIRK
360 370 380 390 400
GVAVGLILRA VNPRDVAYTF LHYSRKMHAR LSPADPNFTR WSVELARIEQ
410 420 430 440 450
WCETYYPSFI AAASEGKPTD IRANALRSWS ESRRTQALIL KQAKLNGSDA
460 470 480 490 500
STLDAKSVLE AAQNSALDPR DLMTEDERAA QDKRDRDQMV KFFLIILVGM
510 520 530 540 550
VTFMGIVALI TWEIVWWWTM DTPDPLSVYV KHAYYLVKTQ GWSTVKEVLR
560 570
TTRLSFEHVW KHGLTSPPKL EL
Length:572
Mass (Da):65,523
Last modified:December 12, 2006 - v2
Checksum:i26FA62E2CA677C10
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti71 – 722EL → DV in CAA68054. 1 PublicationCurated
Sequence conflicti428 – 4281S → T in CAA68054. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X99718 Genomic DNA. Translation: CAA68054.1.
AACP01000154 Genomic DNA. Translation: EAK85649.1.
RefSeqiXP_760521.1. XM_755428.1.

Genome annotation databases

EnsemblFungiiUM04374T0; UM04374P0; UM04374.
GeneIDi3632482.
KEGGiuma:UM04374.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X99718 Genomic DNA. Translation: CAA68054.1.
AACP01000154 Genomic DNA. Translation: EAK85649.1.
RefSeqiXP_760521.1. XM_755428.1.

3D structure databases

ProteinModelPortaliQ92459.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi5270.UM04374.1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiUM04374T0; UM04374P0; UM04374.
GeneIDi3632482.
KEGGiuma:UM04374.1.

Phylogenomic databases

eggNOGiCOG1562.
HOGENOMiHOG000186940.
InParanoidiQ92459.
KOiK00801.
OMAiTFTGNGP.
OrthoDBiEOG7GJ6P5.

Enzyme and pathway databases

UniPathwayiUPA00767; UER00751.

Family and domain databases

Gene3Di1.10.600.10. 1 hit.
InterProiIPR002060. Squ/phyt_synthse.
IPR006449. Squal_synth.
IPR019845. Squalene/phytoene_synthase_CS.
IPR008949. Terpenoid_synth.
[Graphical view]
PfamiPF00494. SQS_PSY. 1 hit.
[Graphical view]
SUPFAMiSSF48576. SSF48576. 1 hit.
TIGRFAMsiTIGR01559. squal_synth. 1 hit.
PROSITEiPS01044. SQUALEN_PHYTOEN_SYN_1. 1 hit.
PS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Squalene synthase in plant pathogenic fungi."
    Corran A.J.
    Submitted (SEP-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: IMI 103761.
  2. "Insights from the genome of the biotrophic fungal plant pathogen Ustilago maydis."
    Kaemper J., Kahmann R., Boelker M., Ma L.-J., Brefort T., Saville B.J., Banuett F., Kronstad J.W., Gold S.E., Mueller O., Perlin M.H., Woesten H.A.B., de Vries R., Ruiz-Herrera J., Reynaga-Pena C.G., Snetselaar K., McCann M., Perez-Martin J.
    , Feldbruegge M., Basse C.W., Steinberg G., Ibeas J.I., Holloman W., Guzman P., Farman M.L., Stajich J.E., Sentandreu R., Gonzalez-Prieto J.M., Kennell J.C., Molina L., Schirawski J., Mendoza-Mendoza A., Greilinger D., Muench K., Roessel N., Scherer M., Vranes M., Ladendorf O., Vincon V., Fuchs U., Sandrock B., Meng S., Ho E.C.H., Cahill M.J., Boyce K.J., Klose J., Klosterman S.J., Deelstra H.J., Ortiz-Castellanos L., Li W., Sanchez-Alonso P., Schreier P.H., Haeuser-Hahn I., Vaupel M., Koopmann E., Friedrich G., Voss H., Schlueter T., Margolis J., Platt D., Swimmer C., Gnirke A., Chen F., Vysotskaia V., Mannhaupt G., Gueldener U., Muensterkoetter M., Haase D., Oesterheld M., Mewes H.-W., Mauceli E.W., DeCaprio D., Wade C.M., Butler J., Young S.K., Jaffe D.B., Calvo S.E., Nusbaum C., Galagan J.E., Birren B.W.
    Nature 444:97-101(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 521 / FGSC 9021.

Entry informationi

Entry nameiFDFT_USTMA
AccessioniPrimary (citable) accession number: Q92459
Secondary accession number(s): Q4P689
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: December 12, 2006
Last modified: January 7, 2015
This is version 103 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.