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Protein

Alpha-galactosidase 2

Gene

agl2

Organism
Hypocrea jecorina (Trichoderma reesei)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Alpha-galactosidase involved in the degradation of simple oligosaccharides like melibiose, raffinose and stachyose, and of polymeric galacto(gluco)mannans.1 Publication

Catalytic activityi

Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids.

pH dependencei

Optimum pH is 3.5-4.5.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei504 – 5041NucleophileBy similarity
Active sitei530 – 5301Proton donorBy similarity

GO - Molecular functioni

  • alpha-galactosidase activity Source: UniProtKB
  • raffinose alpha-galactosidase activity Source: UniProtKB-EC

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Protein family/group databases

CAZyiGH36. Glycoside Hydrolase Family 36.
mycoCLAPiMEL36B_TRIRE.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-galactosidase 2 (EC:3.2.1.22)
Alternative name(s):
Alpha-D-galactoside galactohydrolase 2
Melibiase 2
Gene namesi
Name:agl2
OrganismiHypocrea jecorina (Trichoderma reesei)
Taxonomic identifieri51453 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesHypocreomycetidaeHypocrealesHypocreaceaeTrichoderma

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2626Sequence analysisAdd
BLAST
Chaini27 – 746720Alpha-galactosidase 2PRO_5000147668Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi43 – 431N-linked (GlcNAc...)Sequence analysis
Glycosylationi156 – 1561N-linked (GlcNAc...)Sequence analysis
Glycosylationi180 – 1801N-linked (GlcNAc...)Sequence analysis
Glycosylationi188 – 1881N-linked (GlcNAc...)Sequence analysis
Glycosylationi360 – 3601N-linked (GlcNAc...)Sequence analysis
Glycosylationi427 – 4271N-linked (GlcNAc...)Sequence analysis
Glycosylationi446 – 4461N-linked (GlcNAc...)Sequence analysis
Glycosylationi495 – 4951N-linked (GlcNAc...)Sequence analysis
Glycosylationi714 – 7141N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Interactioni

Protein-protein interaction databases

STRINGi51453.JGI124016.

Structurei

3D structure databases

ProteinModelPortaliQ92457.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 27 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IDX9. Eukaryota.
COG3345. LUCA.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR000111. Glyco_hydro_27/36_CS.
IPR002252. Glyco_hydro_36.
IPR031705. Glyco_hydro_36_C.
IPR031704. Glyco_hydro_36_N.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF16874. Glyco_hydro_36C. 1 hit.
PF16875. Glyco_hydro_36N. 1 hit.
PF02065. Melibiase. 1 hit.
[Graphical view]
PIRSFiPIRSF005536. Agal. 1 hit.
PRINTSiPR00743. GLHYDRLASE36.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00512. ALPHA_GALACTOSIDASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q92457-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLGAPSPRRL ADVLAVTAGL VASVRAASPI SVSGKSFALN GDNVSYRFHV
60 70 80 90 100
DDDSKDLIGD HFGGPATEDG VFPPIIGPIQ GWVDLIGRQR REFPDLGRGD
110 120 130 140 150
FRTPAVHIRQ AAGYTVSDFQ YKSHRVVEGK PALRGLPSTF GDAGDVSTLV
160 170 180 190 200
VHMYDNYSSV AADLTYSIFP KYDAIVRSVN ITNMGKGNIT IEKLASLSVD
210 220 230 240 250
LPYEDFDMLE LKGDWAREGK RLRRKVDYGS QGFGSTTGYS SHLHNPFFSL
260 270 280 290 300
ITPTTTESQG EAWGFSLVYT GSFSVEVEKG SQGLTRAAIG VNPYQLSWPL
310 320 330 340 350
GPGETFSSPE AVAVFSTTGV GGMSRKFHNL YRKHLIKSKF ATQMHPVLLN
360 370 380 390 400
SWEGLGFDYN DTTILHLAQE SADLGIKLFV LDDGWFGVKH PRVSDNAGLG
410 420 430 440 450
DWEANPKRFP QGLPDFISDV TKLKVANSSD HLQFGLWFEP EMVNPNSTLY
460 470 480 490 500
MEHPDWAIHA GSYPRTLTRN QLVLNVALPE VQDFIIESLS NILSNASISY
510 520 530 540 550
VKWDNNRGIH EAPYPGLDYA YMLGLYRVFD TLSSKFPNVR WEGCASGGGR
560 570 580 590 600
FDPGVLQYFP HIWTSDDTDA VERIAIQFGT SLVYPPSAMG AHVSAVPNGQ
610 620 630 640 650
TQRTTSIAFR AHVAMMGGSF GFELTPAEMP EDDKAQIPGI IALAEKVNPI
660 670 680 690 700
VVKGDMWRLS LPEESNWPAA LFISQDGSQA VLFYFQIRAN INNAWPVLRL
710 720 730 740
QGLDASAKYK IDGNQTFSGA TLMNIGLQYQ FNGDYDSKVV FLEKQT
Length:746
Mass (Da):82,079
Last modified:February 1, 1997 - v1
Checksum:i7735B56D7AD3B838
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z69254 mRNA. Translation: CAA93245.1.
PIRiS74219.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z69254 mRNA. Translation: CAA93245.1.
PIRiS74219.

3D structure databases

ProteinModelPortaliQ92457.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi51453.JGI124016.

Protein family/group databases

CAZyiGH36. Glycoside Hydrolase Family 36.
mycoCLAPiMEL36B_TRIRE.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG410IDX9. Eukaryota.
COG3345. LUCA.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR000111. Glyco_hydro_27/36_CS.
IPR002252. Glyco_hydro_36.
IPR031705. Glyco_hydro_36_C.
IPR031704. Glyco_hydro_36_N.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF16874. Glyco_hydro_36C. 1 hit.
PF16875. Glyco_hydro_36N. 1 hit.
PF02065. Melibiase. 1 hit.
[Graphical view]
PIRSFiPIRSF005536. Agal. 1 hit.
PRINTSiPR00743. GLHYDRLASE36.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00512. ALPHA_GALACTOSIDASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Three alpha-galactosidase genes of Trichoderma reesei cloned by expression in yeast."
    Margolles-Clark E., Tenkanen M., Luonteri E., Penttila M.
    Eur. J. Biochem. 240:104-111(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
    Strain: ATCC 56765 / Rut C-30.

Entry informationi

Entry nameiAGAL2_HYPJE
AccessioniPrimary (citable) accession number: Q92457
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 23, 2010
Last sequence update: February 1, 1997
Last modified: November 11, 2015
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.