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Protein

Alpha-galactosidase 2

Gene

agl2

Organism
Hypocrea jecorina (Trichoderma reesei)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Alpha-galactosidase involved in the degradation of simple oligosaccharides like melibiose, raffinose and stachyose, and of polymeric galacto(gluco)mannans.1 Publication

Catalytic activityi

Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids.

pH dependencei

Optimum pH is 3.5-4.5.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei504NucleophileBy similarity1
Active sitei530Proton donorBy similarity1

GO - Molecular functioni

  • alpha-galactosidase activity Source: UniProtKB
  • raffinose alpha-galactosidase activity Source: UniProtKB-EC

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase

Protein family/group databases

CAZyiGH36 Glycoside Hydrolase Family 36
mycoCLAPiMEL36B_TRIRE

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-galactosidase 2 (EC:3.2.1.22)
Alternative name(s):
Alpha-D-galactoside galactohydrolase 2
Melibiase 2
Gene namesi
Name:agl2
OrganismiHypocrea jecorina (Trichoderma reesei)
Taxonomic identifieri51453 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesHypocreomycetidaeHypocrealesHypocreaceaeTrichoderma

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 26Sequence analysisAdd BLAST26
ChainiPRO_500014766827 – 746Alpha-galactosidase 2Add BLAST720

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi43N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi156N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi180N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi188N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi360N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi427N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi446N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi495N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi714N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Structurei

3D structure databases

ProteinModelPortaliQ92457
SMRiQ92457
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 27 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IDX9 Eukaryota
COG3345 LUCA

Family and domain databases

CDDicd14791 GH36, 1 hit
Gene3Di2.60.40.1180, 1 hit
2.70.98.60, 1 hit
3.20.20.70, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR038417 Alpga-gal_N_sf
IPR000111 Glyco_hydro_27/36_CS
IPR002252 Glyco_hydro_36
IPR031705 Glyco_hydro_36_C
IPR031704 Glyco_hydro_36_N
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
PfamiView protein in Pfam
PF16874 Glyco_hydro_36C, 1 hit
PF16875 Glyco_hydro_36N, 1 hit
PF02065 Melibiase, 1 hit
PIRSFiPIRSF005536 Agal, 1 hit
PRINTSiPR00743 GLHYDRLASE36
SUPFAMiSSF51445 SSF51445, 1 hit
PROSITEiView protein in PROSITE
PS00512 ALPHA_GALACTOSIDASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q92457-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLGAPSPRRL ADVLAVTAGL VASVRAASPI SVSGKSFALN GDNVSYRFHV
60 70 80 90 100
DDDSKDLIGD HFGGPATEDG VFPPIIGPIQ GWVDLIGRQR REFPDLGRGD
110 120 130 140 150
FRTPAVHIRQ AAGYTVSDFQ YKSHRVVEGK PALRGLPSTF GDAGDVSTLV
160 170 180 190 200
VHMYDNYSSV AADLTYSIFP KYDAIVRSVN ITNMGKGNIT IEKLASLSVD
210 220 230 240 250
LPYEDFDMLE LKGDWAREGK RLRRKVDYGS QGFGSTTGYS SHLHNPFFSL
260 270 280 290 300
ITPTTTESQG EAWGFSLVYT GSFSVEVEKG SQGLTRAAIG VNPYQLSWPL
310 320 330 340 350
GPGETFSSPE AVAVFSTTGV GGMSRKFHNL YRKHLIKSKF ATQMHPVLLN
360 370 380 390 400
SWEGLGFDYN DTTILHLAQE SADLGIKLFV LDDGWFGVKH PRVSDNAGLG
410 420 430 440 450
DWEANPKRFP QGLPDFISDV TKLKVANSSD HLQFGLWFEP EMVNPNSTLY
460 470 480 490 500
MEHPDWAIHA GSYPRTLTRN QLVLNVALPE VQDFIIESLS NILSNASISY
510 520 530 540 550
VKWDNNRGIH EAPYPGLDYA YMLGLYRVFD TLSSKFPNVR WEGCASGGGR
560 570 580 590 600
FDPGVLQYFP HIWTSDDTDA VERIAIQFGT SLVYPPSAMG AHVSAVPNGQ
610 620 630 640 650
TQRTTSIAFR AHVAMMGGSF GFELTPAEMP EDDKAQIPGI IALAEKVNPI
660 670 680 690 700
VVKGDMWRLS LPEESNWPAA LFISQDGSQA VLFYFQIRAN INNAWPVLRL
710 720 730 740
QGLDASAKYK IDGNQTFSGA TLMNIGLQYQ FNGDYDSKVV FLEKQT
Length:746
Mass (Da):82,079
Last modified:February 1, 1997 - v1
Checksum:i7735B56D7AD3B838
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z69254 mRNA Translation: CAA93245.1
PIRiS74219

Similar proteinsi

Entry informationi

Entry nameiAGAL2_HYPJE
AccessioniPrimary (citable) accession number: Q92457
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 23, 2010
Last sequence update: February 1, 1997
Last modified: March 28, 2018
This is version 73 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

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