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Protein

Alpha-galactosidase 3

Gene

agl3

Organism
Hypocrea jecorina (Trichoderma reesei)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Alpha-galactosidase involved in the degradation of simple oligosaccharides like melibiose, raffinose and stachyose, and of polymeric galacto(gluco)mannans.1 Publication

Catalytic activityi

Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids.

pH dependencei

Optimum pH is 3.5-4.5.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei347NucleophileBy similarity1
Active sitei412Proton donorBy similarity1

GO - Molecular functioni

  • alpha-galactosidase activity Source: UniProtKB
  • raffinose alpha-galactosidase activity Source: UniProtKB-EC

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Protein family/group databases

CAZyiGH27. Glycoside Hydrolase Family 27.
mycoCLAPiMEL27C_TRIRE.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-galactosidase 3 (EC:3.2.1.22)
Alternative name(s):
Alpha-D-galactoside galactohydrolase 3
Melibiase 3
Gene namesi
Name:agl3
OrganismiHypocrea jecorina (Trichoderma reesei)
Taxonomic identifieri51453 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesHypocreomycetidaeHypocrealesHypocreaceaeTrichoderma

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Sequence analysisAdd BLAST22
ChainiPRO_500014766923 – 624Alpha-galactosidase 3Add BLAST602

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi37N-linked (GlcNAc...)Sequence analysis1
Glycosylationi56N-linked (GlcNAc...)Sequence analysis1
Glycosylationi197N-linked (GlcNAc...)Sequence analysis1
Glycosylationi259N-linked (GlcNAc...)Sequence analysis1
Glycosylationi293N-linked (GlcNAc...)Sequence analysis1
Glycosylationi393N-linked (GlcNAc...)Sequence analysis1
Glycosylationi469N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Interactioni

Protein-protein interaction databases

STRINGi51453.JGI72704.

Structurei

3D structure databases

ProteinModelPortaliQ92451.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 27 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410JAVI. Eukaryota.
ENOG410Y110. LUCA.
OMAiANLACDS.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR013780. Glyco_hydro_b.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q92451-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSPSAAVLIP LAAAVLLRPV VGQTQCGGNL YTPGTLNFTL ECYNAFQDCV
60 70 80 90 100
AQFEANASQV DCNDGKGNLF MQQQANLGAS PGSQNNDAII AFQDIRDLCL
110 120 130 140 150
LSGSTTATWG YSDNQWYWAA AEDACYTNDP TRTDVVKTHP APFCIQNRDS
160 170 180 190 200
SLPECYPQPD ATPPGGPLKV IKTAKTRNGF KSSARGWNTY GVQALVNGSQ
210 220 230 240 250
VVPSFAGQSG LFYTQKFVET QCGVLARPEF KKAGYDLCSL DSGWQATTAV
260 270 280 290 300
DQHGRIIYNT TRFNLPELAS WLHKRDLKLG VYITPGVPCL AHNQTILGTN
310 320 330 340 350
IKIKDVLNGN NDQINCDFDF RKDGVQQWHD SVVAQWASWG VDMLKLDFLT
360 370 380 390 400
PGSPSNGANL ACDSSDAVRA YQKAIKKSGR KIRLDISWKL CRNETWLPIW
410 420 430 440 450
SDLAESMRTD QDLDNYGTNT LMAWQVGQRA IENYRQYIGL QAQRNVPLTI
460 470 480 490 500
YPDMDALFTV NPEHLAGVND TIRYTVQNHW LGAGANLIIG GDMEQVDALG
510 520 530 540 550
LKLTTSKQSI DAADFFAKYP MQPRNPGTGS NAAKQLQAWI GGPSDDHEAY
560 570 580 590 600
VLIVNYGPDL GNGGFSTKLY GKQKVTVSLK DLGISGSAWT FTDIWSGKSS
610 620
RVTGSYSAWL TEGESQLLRL KRTH
Length:624
Mass (Da):68,455
Last modified:February 1, 1997 - v1
Checksum:i9454A5C932040D73
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z69255 mRNA. Translation: CAA93246.1.
PIRiS74222.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z69255 mRNA. Translation: CAA93246.1.
PIRiS74222.

3D structure databases

ProteinModelPortaliQ92451.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi51453.JGI72704.

Protein family/group databases

CAZyiGH27. Glycoside Hydrolase Family 27.
mycoCLAPiMEL27C_TRIRE.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG410JAVI. Eukaryota.
ENOG410Y110. LUCA.
OMAiANLACDS.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR013780. Glyco_hydro_b.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiAGAL3_HYPJE
AccessioniPrimary (citable) accession number: Q92451
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 23, 2010
Last sequence update: February 1, 1997
Last modified: July 6, 2016
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.