Reviewed, UniProtKB/Swiss-Prot Q92448 (K6PF1_PICPA)

Names and origin

Protein names

Recommended name:
    6-phosphofructokinase
      Short name=Phosphofructokinase
    EC=2.7.1.11
Alternative name(s):
    Phosphohexokinase

Gene names

Name:

PFK1

Organism

Pichia pastoris (Yeast)

Taxonomic identifier

4922 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Pichia

Protein attributes

Sequence length	990 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.
Subunit structure	Heterooctamer of 4 alpha and 4 beta chains By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the phosphofructokinase family. Two domains subfamily.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Domain	Repeat
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Allosteric enzyme
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	6-phosphofructokinase complex Inferred from electronic annotation. Source: InterPro
Molecular function	6-phosphofructokinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 990	990	6-phosphofructokinase		PRO_0000112042
Regions
	Nucleotide binding	231 – 235	5	ATP By similarity
	Nucleotide binding	389 – 393	5	ATP By similarity
	Nucleotide binding	406 – 422	17	ATP By similarity
Sites
	Active site	362	1	Proton acceptor By similarity
	Metal binding	420	1	Magnesium; via carbonyl oxygen By similarity
	Binding site	397	1	Substrate By similarity
	Binding site	488	1	Substrate By similarity
	Binding site	494	1	Substrate By similarity
	Binding site	497	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q92448-1 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 307CB9FBC2C269AF	FASTA	990	108,899
	10 20 30 40 50 60 MPEPSISALS FTSFVTNDDK LFEETFNFYT KLGFHATRSY VKDNRSDFEL TGISTDSIKE 70 80 90 100 110 120 IWLESFPLSE VVETSAGREL RKPLQESVGY QSEALLGYSP YQSDGVVIKL RLSNHDLQKN 130 140 150 160 170 180 KDLPGEVTFF TASIDKLRAK LIEIGAEIIP SEIDLVEFST KDPMGDVISF SSYPSLSSKK 190 200 210 220 230 240 KITSPDFFLH PKKEVRSQES IVEQVKSEEG KKKIAIITSG GDAPGMNAAV RAVTRAGIFY 250 260 270 280 290 300 GCKVYACYEG YTGLVKGGDM LKELQWQDVR GLLSIGGTII GTARSKEFRE RWGRLQACYN 310 320 330 340 350 360 MVSNGIDALV VCGGDGSLTG ADLFRNEWPE LIKELLGEGK ITKEQYETHR NLTIVGLVGS 370 380 390 400 410 420 IDNDMCGTDS TIGAYSSLER IIELVDYIDA TAASHSRAFV VEVMGRHCGW LGLMSGIATG 430 440 450 460 470 480 ADYIFIPERP PSETNWKDDL KKVCLRHREK GRRKTTVIVA EGAIDDQLNP ITSEEVKDVL 490 500 510 520 530 540 VEIGLDTRIT RLGHVQRGGA PCAFDRFLAT VQGVDAVRAV LESTPAIPSP VISILENKIV 550 560 570 580 590 600 RQPLVESVAQ TKTVSDAIEA KDFDKALKLR EQEFATSYES FLSVSKYDDG SYLVPESSRL 610 620 630 640 650 660 NIAIIHVGAP TSALNPATRV ATLNSLAKGH RVFAIRNGFA GLIRHGAVRE LNWIDVEDWH 670 680 690 700 710 720 NTGGSEIGTN RSLPSDDMGT VAYYFQQYKF DGLIIIGGFE AFTALYQLDA ARAQYPIFNI 730 740 750 760 770 780 PMCCLPATVS NNVPGTEYSL GSDTCLNTLS GYCDAVKQSA SASRRRTFVV EVQGGYSGYL 790 800 810 820 830 840 ASYAGLITGA LAVYTPENPI NLQTVQEDIE LLTRTYEEDD GKNRSGKIFI HNEKASKVYT 850 860 870 880 890 900 TDLIAAIIGE AGKGRFESRT AVPGHVQQGK SPSSIDRVNA CRLAIKCCNF IEDANFQVKH 910 920 930 940 950 960 NANLSADERH LRFFCDDGVK TSAVSGKSSV IDDNTSVVIG IQGSEVTFTP VKQLWEKETH 970 980 990 HKWRKGKNVH WEQLNIVSDL LSGRLSIRTT

References

[1]	"Glucose-induced microautophagy in Pichia pastoris requires the alpha-subunit of phosphofructokinase." Yuan W., Tuttle D.L., Shi Y.J., Ralph G.S., Dunn W.A. Jr. J. Cell Sci. 110:1935-1945(1997) [PubMed: 9296392] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	U73376 Genomic DNA. Translation: AAB97871.1.
3D structure databases
HSSP	HSSP built from PDB template 3PFK based on UniProtKB P00512.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.7.1.11. 390.
Family and domain databases
InterPro	IPR009161. 6-phosphofructokinase_euk. IPR000023. Phosphofructokinase. IPR015912. Phosphofructokinase_CS. [Graphical view]
Pfam	PF00365. PFK. 2 hits. [Graphical view]
PIRSF	PIRSF000533. ATP_PFK_euk. 1 hit.
PRINTS	PR00476. PHFRCTKINASE.
ProDom	PD000707. Ppfruckinase. 2 hits. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR02478. 6PF1K_euk. 1 hit.
PROSITE	PS00433. PHOSPHOFRUCTOKINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

K6PF1_PICPA

Accession

Primary (citable) accession number: Q92448

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	June 16, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)