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Q92448 (K6PF1_PICPA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
6-phosphofructokinase

Short name=Phosphofructokinase
EC=2.7.1.11
Alternative name(s):
Phosphohexokinase
Gene names
Name:PFK1
OrganismKomagataella pastoris (Yeast) (Pichia pastoris)
Taxonomic identifier4922 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeKomagataella

Protein attributes

Sequence length990 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. HAMAP-Rule MF_00339

Pathway

Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4. HAMAP-Rule MF_00339

Subunit structure

Heterooctamer of 4 alpha and 4 beta chains By similarity.

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00339.

Sequence similarities

Belongs to the phosphofructokinase family. Two domains subfamily.

Ontologies

Keywords
   Biological processGlycolysis
   Cellular componentCytoplasm
   DomainRepeat
   LigandATP-binding
Magnesium
Metal-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
   Technical termAllosteric enzyme
Gene Ontology (GO)
   Biological_processfructose 6-phosphate metabolic process

Inferred from electronic annotation. Source: InterPro

glycolysis

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_component6-phosphofructokinase complex

Inferred from electronic annotation. Source: InterPro

   Molecular_function6-phosphofructokinase activity

Inferred from electronic annotation. Source: UniProtKB-EC

ATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 9909906-phosphofructokinase HAMAP-Rule MF_00339
PRO_0000112042

Regions

Nucleotide binding231 – 2355ATP By similarity
Nucleotide binding389 – 3935ATP By similarity
Nucleotide binding406 – 42217ATP By similarity

Sites

Active site3621Proton acceptor By similarity
Metal binding4201Magnesium; via carbonyl oxygen By similarity
Binding site3971Substrate By similarity
Binding site4881Substrate By similarity
Binding site4941Substrate By similarity
Binding site4971Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q92448 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: 307CB9FBC2C269AF

FASTA990108,899
        10         20         30         40         50         60 
MPEPSISALS FTSFVTNDDK LFEETFNFYT KLGFHATRSY VKDNRSDFEL TGISTDSIKE 

        70         80         90        100        110        120 
IWLESFPLSE VVETSAGREL RKPLQESVGY QSEALLGYSP YQSDGVVIKL RLSNHDLQKN 

       130        140        150        160        170        180 
KDLPGEVTFF TASIDKLRAK LIEIGAEIIP SEIDLVEFST KDPMGDVISF SSYPSLSSKK 

       190        200        210        220        230        240 
KITSPDFFLH PKKEVRSQES IVEQVKSEEG KKKIAIITSG GDAPGMNAAV RAVTRAGIFY 

       250        260        270        280        290        300 
GCKVYACYEG YTGLVKGGDM LKELQWQDVR GLLSIGGTII GTARSKEFRE RWGRLQACYN 

       310        320        330        340        350        360 
MVSNGIDALV VCGGDGSLTG ADLFRNEWPE LIKELLGEGK ITKEQYETHR NLTIVGLVGS 

       370        380        390        400        410        420 
IDNDMCGTDS TIGAYSSLER IIELVDYIDA TAASHSRAFV VEVMGRHCGW LGLMSGIATG 

       430        440        450        460        470        480 
ADYIFIPERP PSETNWKDDL KKVCLRHREK GRRKTTVIVA EGAIDDQLNP ITSEEVKDVL 

       490        500        510        520        530        540 
VEIGLDTRIT RLGHVQRGGA PCAFDRFLAT VQGVDAVRAV LESTPAIPSP VISILENKIV 

       550        560        570        580        590        600 
RQPLVESVAQ TKTVSDAIEA KDFDKALKLR EQEFATSYES FLSVSKYDDG SYLVPESSRL 

       610        620        630        640        650        660 
NIAIIHVGAP TSALNPATRV ATLNSLAKGH RVFAIRNGFA GLIRHGAVRE LNWIDVEDWH 

       670        680        690        700        710        720 
NTGGSEIGTN RSLPSDDMGT VAYYFQQYKF DGLIIIGGFE AFTALYQLDA ARAQYPIFNI 

       730        740        750        760        770        780 
PMCCLPATVS NNVPGTEYSL GSDTCLNTLS GYCDAVKQSA SASRRRTFVV EVQGGYSGYL 

       790        800        810        820        830        840 
ASYAGLITGA LAVYTPENPI NLQTVQEDIE LLTRTYEEDD GKNRSGKIFI HNEKASKVYT 

       850        860        870        880        890        900 
TDLIAAIIGE AGKGRFESRT AVPGHVQQGK SPSSIDRVNA CRLAIKCCNF IEDANFQVKH 

       910        920        930        940        950        960 
NANLSADERH LRFFCDDGVK TSAVSGKSSV IDDNTSVVIG IQGSEVTFTP VKQLWEKETH 

       970        980        990 
HKWRKGKNVH WEQLNIVSDL LSGRLSIRTT 

« Hide

References

[1]"Glucose-induced microautophagy in Pichia pastoris requires the alpha-subunit of phosphofructokinase."
Yuan W., Tuttle D.L., Shi Y.J., Ralph G.S., Dunn W.A. Jr.
J. Cell Sci. 110:1935-1945(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U73376 Genomic DNA. Translation: AAB97871.1.

3D structure databases

ProteinModelPortalQ92448.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00109; UER00182.

Family and domain databases

HAMAPMF_00339. Phosphofructokinase.
InterProIPR009161. 6-phosphofructokinase_euk.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamPF00365. PFK. 2 hits.
[Graphical view]
PIRSFPIRSF000533. ATP_PFK_euk. 1 hit.
PRINTSPR00476. PHFRCTKINASE.
SUPFAMSSF53784. SSF53784. 3 hits.
TIGRFAMsTIGR02478. 6PF1K_euk. 1 hit.
PROSITEPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameK6PF1_PICPA
AccessionPrimary (citable) accession number: Q92448
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: April 16, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways