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Protein

ATP-dependent 6-phosphofructokinase subunit alpha

Gene

PFK1

Organism
Komagataella phaffii (strain GS115 / ATCC 20864) (Yeast) (Pichia pastoris)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis (By similarity). Involved in the modulation of glucose-induced microautophagy of peroxisomes independent of its ability to metabolize glucose intermediates.UniRule annotation1 Publication

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Allosterically activated by ADP, AMP, or fructose 2,6-bisphosphate, and allosterically inhibited by ATP or citrate.UniRule annotation

Pathwayi: glycolysis

This protein is involved in step 3 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (PAS_chr3_0456)
  3. ATP-dependent 6-phosphofructokinase subunit gamma (PFK3), ATP-dependent 6-phosphofructokinase subunit beta (PFK2), ATP-dependent 6-phosphofructokinase subunit alpha (PFK1)
  4. no protein annotated in this organism
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei220ATP; via amide nitrogenUniRule annotation1
Metal bindingi314Magnesium; catalyticUniRule annotation1
Active sitei361Proton acceptorUniRule annotation1
Binding sitei396Substrate; shared with subunit betaUniRule annotation1
Binding sitei460SubstrateUniRule annotation1
Binding sitei487Substrate; shared with subunit betaUniRule annotation1
Binding sitei670Allosteric activator fructose 2,6-bisphosphateUniRule annotation1
Binding sitei765Allosteric activator fructose 2,6-bisphosphate; shared with subunit betaUniRule annotation1
Binding sitei832Allosteric activator fructose 2,6-bisphosphateUniRule annotation1
Binding sitei858Allosteric activator fructose 2,6-bisphosphate; shared with subunit betaUniRule annotation1
Binding sitei963Allosteric activator fructose 2,6-bisphosphateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi283 – 284ATPUniRule annotation2
Nucleotide bindingi313 – 316ATPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinase subunit alphaUniRule annotation (EC:2.7.1.11UniRule annotation)
Alternative name(s):
ATP-dependent 6-phosphofructokinase 1UniRule annotation
Short name:
ATP-PFK 1UniRule annotation
Short name:
Phosphofructokinase 1UniRule annotation
Glucose-induced selective autophagy 1 protein
Phosphohexokinase 1UniRule annotation
Gene namesi
Name:PFK1
Synonyms:GSA1
Ordered Locus Names:PAS_chr2-1_0402
OrganismiKomagataella phaffii (strain GS115 / ATCC 20864) (Yeast) (Pichia pastoris)
Taxonomic identifieri644223 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesPhaffomycetaceaeKomagataella
Proteomesi
  • UP000000314 Componenti: Chromosome 2

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi361D → S: Abolishes catalytic activity, but not the ability to modulate glucose-induced microautophagy of peroxisomes. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001120421 – 989ATP-dependent 6-phosphofructokinase subunit alphaAdd BLAST989

Interactioni

Subunit structurei

Heterododecamer of 4 alpha, 4 beta and 4 gamma chains. The gamma chain bridges the N-terminal halves of the alpha and beta subunits (By similarity).By similarity

Protein-protein interaction databases

STRINGi644223.XP_002491303.1.

Structurei

3D structure databases

ProteinModelPortaliQ92448.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 585N-terminal catalytic PFK domain 1Add BLAST585
Regioni359 – 361Substrate bindingUniRule annotation3
Regioni403 – 405Substrate bindingUniRule annotation3
Regioni493 – 496Substrate bindingUniRule annotation4
Regioni586 – 599Interdomain linkerAdd BLAST14
Regioni600 – 989C-terminal regulatory PFK domain 2Add BLAST390
Regioni727 – 731Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation5
Regioni772 – 774Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation3
Regioni864 – 867Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade "E" sub-subfamily.UniRule annotation

Phylogenomic databases

eggNOGiKOG2440. Eukaryota.
COG0205. LUCA.
HOGENOMiHOG000200154.
KOiK00850.
OMAiWENETNI.

Family and domain databases

HAMAPiMF_03184. Phosphofructokinase_I_E. 1 hit.
InterProiIPR009161. 6-Pfructokinase_euk.
IPR022953. ATP_PFK.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 2 hits.
[Graphical view]
PIRSFiPIRSF000533. ATP_PFK_euk. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 3 hits.
TIGRFAMsiTIGR02478. 6PF1K_euk. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q92448-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPEPSISALS FTSFVTNDDK LFEETFNFYT KLGFHATRSY VKDNRSDFEL
60 70 80 90 100
TGISTDSIKE IWLESFPLSE VVETSAGREL RKPLQESVGY QSEALLGYSP
110 120 130 140 150
YQSDGVVIKL RLSNHDLQKN KDLPGEVTFF TASIDKLRAK LIEIGAEIIP
160 170 180 190 200
SEIDLVEFST KDPMGDVISF SSYPSLSSKK ITSPDFFLHP KKEVRSQESI
210 220 230 240 250
VEQVKSEEGK KKIAIITSGG DAPGMNAAVR AVTRAGIFYG CKVYACYEGY
260 270 280 290 300
TGLVKGGDML KELQWQDVRG LLSIGGTIIG TARSKEFRER WGRLQACYNM
310 320 330 340 350
VSNGIDALVV CGGDGSLTGA DLFRNEWPEL IKELLGEGKI TKEQYETHRN
360 370 380 390 400
LTIVGLVGSI DNDMCGTDST IGAYSSLERI IELVDYIDAT AASHSRAFVV
410 420 430 440 450
EVMGRHCGWL GLMSGIATGA DYIFIPERPP SETNWKDDLK KVCLRHREKG
460 470 480 490 500
RRKTTVIVAE GAIDDQLNPI TSEEVKDVLV EIGLDTRITR LGHVQRGGAP
510 520 530 540 550
CAFDRFLATV QGVDAVRAVL ESTPAIPSPV ISILENKIVR QPLVESVAQT
560 570 580 590 600
KTVSDAIEAK DFDKALKLRD QEFATSYESF LSVSKYDDGS YLVPESSRLN
610 620 630 640 650
IAIIHVGAPT SALNPATRVA TLNSLAKGHR VFAIRNGFAG LIRHGAVREL
660 670 680 690 700
NWIDVEDWHN TGGSEIGTNR SLPSDDMGTV AYYFQQYKFD GLIIIGGFEA
710 720 730 740 750
FTALYQLDAA RAQYPIFNIP MCCLPATVSN NVPGTEYSLG SDTCLNTLSG
760 770 780 790 800
YCDAVKQSAS ASRRRTFVVE VQGGYSGYLA SYAGLITGAL AVYTPENPIN
810 820 830 840 850
LQTVQEDIEL LTRTYEEDDG KNRSGKIFIH NEKASKVYTT DLIAAIIGEA
860 870 880 890 900
GKGRFESRTA VPGHVQQGKS PSSIDRVNAC RLAIKCCNFI EDANFQVKHN
910 920 930 940 950
ANLSADERHL RFFYDDGVKT SAVSGKSSVI DDNTSVVIGI QGSEVTFTPV
960 970 980
KQLWEKETHH KWRKGKNVHW EQLNIVSDLL SGRLSIRTT
Length:989
Mass (Da):108,817
Last modified:July 9, 2014 - v2
Checksum:i6708A34ED94C9DD4
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti178S → SK in AAB97871 (PubMed:9296392).Curated1
Sequence conflicti570D → E in AAB97871 (PubMed:9296392).Curated1
Sequence conflicti914Y → C in AAB97871 (PubMed:9296392).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U73376 Genomic DNA. Translation: AAB97871.1.
FN392320 Genomic DNA. Translation: CAY69023.1.
RefSeqiXP_002491303.1. XM_002491258.1.

Genome annotation databases

EnsemblFungiiCAY69023; CAY69023; PAS_chr2-1_0402.
GeneIDi8198870.
KEGGippa:PAS_chr2-1_0402.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U73376 Genomic DNA. Translation: AAB97871.1.
FN392320 Genomic DNA. Translation: CAY69023.1.
RefSeqiXP_002491303.1. XM_002491258.1.

3D structure databases

ProteinModelPortaliQ92448.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi644223.XP_002491303.1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCAY69023; CAY69023; PAS_chr2-1_0402.
GeneIDi8198870.
KEGGippa:PAS_chr2-1_0402.

Phylogenomic databases

eggNOGiKOG2440. Eukaryota.
COG0205. LUCA.
HOGENOMiHOG000200154.
KOiK00850.
OMAiWENETNI.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.

Family and domain databases

HAMAPiMF_03184. Phosphofructokinase_I_E. 1 hit.
InterProiIPR009161. 6-Pfructokinase_euk.
IPR022953. ATP_PFK.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 2 hits.
[Graphical view]
PIRSFiPIRSF000533. ATP_PFK_euk. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 3 hits.
TIGRFAMsiTIGR02478. 6PF1K_euk. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPFKA1_KOMPG
AccessioniPrimary (citable) accession number: Q92448
Secondary accession number(s): C4R0J9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 9, 2014
Last modified: November 2, 2016
This is version 88 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.