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Q92448

- PFKA1_PICPG

UniProt

Q92448 - PFKA1_PICPG

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Protein
ATP-dependent 6-phosphofructokinase subunit alpha
Gene
PFK1, GSA1, PAS_chr2-1_0402
Organism
Komagataella pastoris (strain GS115 / ATCC 20864) (Yeast) (Pichia pastoris)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis By similarity. Involved in the modulation of glucose-induced microautophagy of peroxisomes independent of its ability to metabolize glucose intermediates.1 Publication

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Enzyme regulationi

Allosterically activated by ADP, AMP, or fructose 2,6-bisphosphate, and allosterically inhibited by ATP or citrate By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei220 – 2201ATP; via amide nitrogen By similarity
Metal bindingi314 – 3141Magnesium; catalytic By similarity
Active sitei361 – 3611Proton acceptor By similarity
Binding sitei396 – 3961Substrate; shared with subunit beta By similarity
Binding sitei460 – 4601Substrate By similarity
Binding sitei487 – 4871Substrate; shared with subunit beta By similarity
Binding sitei670 – 6701Allosteric activator fructose 2,6-bisphosphate By similarity
Binding sitei765 – 7651Allosteric activator fructose 2,6-bisphosphate; shared with subunit beta By similarity
Binding sitei832 – 8321Allosteric activator fructose 2,6-bisphosphate By similarity
Binding sitei858 – 8581Allosteric activator fructose 2,6-bisphosphate; shared with subunit beta By similarity
Binding sitei963 – 9631Allosteric activator fructose 2,6-bisphosphate By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi283 – 2842ATP By similarity
Nucleotide bindingi313 – 3164ATP By similarity

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: UniProtKB-EC
  2. ATP binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. fructose 6-phosphate metabolic process Source: InterPro
  2. glycolytic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinase subunit alpha (EC:2.7.1.11)
Alternative name(s):
ATP-dependent 6-phosphofructokinase 1
Short name:
ATP-PFK 1
Short name:
Phosphofructokinase 1
Glucose-induced selective autophagy 1 protein
Phosphohexokinase 1
Gene namesi
Name:PFK1
Synonyms:GSA1
Ordered Locus Names:PAS_chr2-1_0402
OrganismiKomagataella pastoris (strain GS115 / ATCC 20864) (Yeast) (Pichia pastoris)
Taxonomic identifieri644223 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeKomagataella
ProteomesiUP000000314: Chromosome 2

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. 6-phosphofructokinase complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi361 – 3611D → S: Abolishes catalytic activity, but not the ability to modulate glucose-induced microautophagy of peroxisomes. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 989989ATP-dependent 6-phosphofructokinase subunit alphaUniRule annotation
PRO_0000112042Add
BLAST

Interactioni

Subunit structurei

Heterododecamer of 4 alpha, 4 beta and 4 gamma chains. The gamma chain bridges the N-terminal halves of the alpha and beta subunits By similarity.

Protein-protein interaction databases

STRINGi644223.PAS_chr2-1_0402.

Structurei

3D structure databases

ProteinModelPortaliQ92448.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 585585N-terminal catalytic PFK domain 1UniRule annotation
Add
BLAST
Regioni359 – 3613Substrate binding By similarity
Regioni403 – 4053Substrate binding By similarity
Regioni493 – 4964Substrate binding By similarity
Regioni586 – 59914Interdomain linkerUniRule annotation
Add
BLAST
Regioni600 – 989390C-terminal regulatory PFK domain 2UniRule annotation
Add
BLAST
Regioni727 – 7315Allosteric activator fructose 2,6-bisphosphate binding By similarity
Regioni772 – 7743Allosteric activator fructose 2,6-bisphosphate binding By similarity
Regioni864 – 8674Allosteric activator fructose 2,6-bisphosphate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0205.
KOiK00850.
OrthoDBiEOG7Q5HPV.

Family and domain databases

HAMAPiMF_03184. Phosphofructokinase_I_E.
InterProiIPR009161. 6-phosphofructokinase_euk.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 2 hits.
[Graphical view]
PIRSFiPIRSF000533. ATP_PFK_euk. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 3 hits.
TIGRFAMsiTIGR02478. 6PF1K_euk. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q92448-1 [UniParc]FASTAAdd to Basket

« Hide

MPEPSISALS FTSFVTNDDK LFEETFNFYT KLGFHATRSY VKDNRSDFEL    50
TGISTDSIKE IWLESFPLSE VVETSAGREL RKPLQESVGY QSEALLGYSP 100
YQSDGVVIKL RLSNHDLQKN KDLPGEVTFF TASIDKLRAK LIEIGAEIIP 150
SEIDLVEFST KDPMGDVISF SSYPSLSSKK ITSPDFFLHP KKEVRSQESI 200
VEQVKSEEGK KKIAIITSGG DAPGMNAAVR AVTRAGIFYG CKVYACYEGY 250
TGLVKGGDML KELQWQDVRG LLSIGGTIIG TARSKEFRER WGRLQACYNM 300
VSNGIDALVV CGGDGSLTGA DLFRNEWPEL IKELLGEGKI TKEQYETHRN 350
LTIVGLVGSI DNDMCGTDST IGAYSSLERI IELVDYIDAT AASHSRAFVV 400
EVMGRHCGWL GLMSGIATGA DYIFIPERPP SETNWKDDLK KVCLRHREKG 450
RRKTTVIVAE GAIDDQLNPI TSEEVKDVLV EIGLDTRITR LGHVQRGGAP 500
CAFDRFLATV QGVDAVRAVL ESTPAIPSPV ISILENKIVR QPLVESVAQT 550
KTVSDAIEAK DFDKALKLRD QEFATSYESF LSVSKYDDGS YLVPESSRLN 600
IAIIHVGAPT SALNPATRVA TLNSLAKGHR VFAIRNGFAG LIRHGAVREL 650
NWIDVEDWHN TGGSEIGTNR SLPSDDMGTV AYYFQQYKFD GLIIIGGFEA 700
FTALYQLDAA RAQYPIFNIP MCCLPATVSN NVPGTEYSLG SDTCLNTLSG 750
YCDAVKQSAS ASRRRTFVVE VQGGYSGYLA SYAGLITGAL AVYTPENPIN 800
LQTVQEDIEL LTRTYEEDDG KNRSGKIFIH NEKASKVYTT DLIAAIIGEA 850
GKGRFESRTA VPGHVQQGKS PSSIDRVNAC RLAIKCCNFI EDANFQVKHN 900
ANLSADERHL RFFYDDGVKT SAVSGKSSVI DDNTSVVIGI QGSEVTFTPV 950
KQLWEKETHH KWRKGKNVHW EQLNIVSDLL SGRLSIRTT 989
Length:989
Mass (Da):108,817
Last modified:July 9, 2014 - v2
Checksum:i6708A34ED94C9DD4
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti178 – 1781S → SK in AAB97871. 1 Publication
Sequence conflicti570 – 5701D → E in AAB97871. 1 Publication
Sequence conflicti914 – 9141Y → C in AAB97871. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U73376 Genomic DNA. Translation: AAB97871.1.
FN392320 Genomic DNA. Translation: CAY69023.1.
RefSeqiXP_002491303.1. XM_002491258.1.

Genome annotation databases

EnsemblFungiiCAY69023; CAY69023; PAS_chr2-1_0402.
GeneIDi8198870.
KEGGippa:PAS_chr2-1_0402.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U73376 Genomic DNA. Translation: AAB97871.1 .
FN392320 Genomic DNA. Translation: CAY69023.1 .
RefSeqi XP_002491303.1. XM_002491258.1.

3D structure databases

ProteinModelPortali Q92448.
ModBasei Search...

Protein-protein interaction databases

STRINGi 644223.PAS_chr2-1_0402.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii CAY69023 ; CAY69023 ; PAS_chr2-1_0402 .
GeneIDi 8198870.
KEGGi ppa:PAS_chr2-1_0402.

Phylogenomic databases

eggNOGi COG0205.
KOi K00850.
OrthoDBi EOG7Q5HPV.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00182 .

Family and domain databases

HAMAPi MF_03184. Phosphofructokinase_I_E.
InterProi IPR009161. 6-phosphofructokinase_euk.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view ]
Pfami PF00365. PFK. 2 hits.
[Graphical view ]
PIRSFi PIRSF000533. ATP_PFK_euk. 1 hit.
PRINTSi PR00476. PHFRCTKINASE.
SUPFAMi SSF53784. SSF53784. 3 hits.
TIGRFAMsi TIGR02478. 6PF1K_euk. 1 hit.
PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Glucose-induced microautophagy in Pichia pastoris requires the alpha-subunit of phosphofructokinase."
    Yuan W., Tuttle D.L., Shi Y.J., Ralph G.S., Dunn W.A. Jr.
    J. Cell Sci. 110:1935-1945(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, MUTAGENESIS OF ASP-361.
    Strain: GS115 / ATCC 20864.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: GS115 / ATCC 20864.

Entry informationi

Entry nameiPFKA1_PICPG
AccessioniPrimary (citable) accession number: Q92448
Secondary accession number(s): C4R0J9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 9, 2014
Last modified: September 3, 2014
This is version 76 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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