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Q92448

- PFKA1_PICPG

UniProt

Q92448 - PFKA1_PICPG

Protein

ATP-dependent 6-phosphofructokinase subunit alpha

Gene

PFK1

Organism
Komagataella pastoris (strain GS115 / ATCC 20864) (Yeast) (Pichia pastoris)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 77 (01 Oct 2014)
      Sequence version 2 (09 Jul 2014)
      Previous versions | rss
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    Functioni

    Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis By similarity. Involved in the modulation of glucose-induced microautophagy of peroxisomes independent of its ability to metabolize glucose intermediates.1 PublicationUniRule annotation

    Catalytic activityi

    ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Enzyme regulationi

    Allosterically activated by ADP, AMP, or fructose 2,6-bisphosphate, and allosterically inhibited by ATP or citrate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei220 – 2201ATP; via amide nitrogenUniRule annotation
    Metal bindingi314 – 3141Magnesium; catalyticUniRule annotation
    Active sitei361 – 3611Proton acceptorUniRule annotation
    Binding sitei396 – 3961Substrate; shared with subunit betaUniRule annotation
    Binding sitei460 – 4601SubstrateUniRule annotation
    Binding sitei487 – 4871Substrate; shared with subunit betaUniRule annotation
    Binding sitei670 – 6701Allosteric activator fructose 2,6-bisphosphateUniRule annotation
    Binding sitei765 – 7651Allosteric activator fructose 2,6-bisphosphate; shared with subunit betaUniRule annotation
    Binding sitei832 – 8321Allosteric activator fructose 2,6-bisphosphateUniRule annotation
    Binding sitei858 – 8581Allosteric activator fructose 2,6-bisphosphate; shared with subunit betaUniRule annotation
    Binding sitei963 – 9631Allosteric activator fructose 2,6-bisphosphateUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi283 – 2842ATPUniRule annotation
    Nucleotide bindingi313 – 3164ATPUniRule annotation

    GO - Molecular functioni

    1. 6-phosphofructokinase activity Source: UniProtKB-EC
    2. ATP binding Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. fructose 6-phosphate metabolic process Source: InterPro
    2. glycolytic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00109; UER00182.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ATP-dependent 6-phosphofructokinase subunit alphaUniRule annotation (EC:2.7.1.11UniRule annotation)
    Alternative name(s):
    ATP-dependent 6-phosphofructokinase 1UniRule annotation
    Short name:
    ATP-PFK 1UniRule annotation
    Short name:
    Phosphofructokinase 1UniRule annotation
    Glucose-induced selective autophagy 1 protein
    Phosphohexokinase 1UniRule annotation
    Gene namesi
    Name:PFK1
    Synonyms:GSA1
    Ordered Locus Names:PAS_chr2-1_0402
    OrganismiKomagataella pastoris (strain GS115 / ATCC 20864) (Yeast) (Pichia pastoris)
    Taxonomic identifieri644223 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeKomagataella
    ProteomesiUP000000314: Chromosome 2

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. 6-phosphofructokinase complex Source: InterPro

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi361 – 3611D → S: Abolishes catalytic activity, but not the ability to modulate glucose-induced microautophagy of peroxisomes. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 989989ATP-dependent 6-phosphofructokinase subunit alphaPRO_0000112042Add
    BLAST

    Interactioni

    Subunit structurei

    Heterododecamer of 4 alpha, 4 beta and 4 gamma chains. The gamma chain bridges the N-terminal halves of the alpha and beta subunits By similarity.By similarity

    Protein-protein interaction databases

    STRINGi644223.PAS_chr2-1_0402.

    Structurei

    3D structure databases

    ProteinModelPortaliQ92448.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 585585N-terminal catalytic PFK domain 1Add
    BLAST
    Regioni359 – 3613Substrate bindingUniRule annotation
    Regioni403 – 4053Substrate bindingUniRule annotation
    Regioni493 – 4964Substrate bindingUniRule annotation
    Regioni586 – 59914Interdomain linkerAdd
    BLAST
    Regioni600 – 989390C-terminal regulatory PFK domain 2Add
    BLAST
    Regioni727 – 7315Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation
    Regioni772 – 7743Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation
    Regioni864 – 8674Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade "E" sub-subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0205.
    KOiK00850.
    OrthoDBiEOG7Q5HPV.

    Family and domain databases

    HAMAPiMF_03184. Phosphofructokinase_I_E.
    InterProiIPR009161. 6-phosphofructokinase_euk.
    IPR022953. Phosphofructokinase.
    IPR015912. Phosphofructokinase_CS.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view]
    PfamiPF00365. PFK. 2 hits.
    [Graphical view]
    PIRSFiPIRSF000533. ATP_PFK_euk. 1 hit.
    PRINTSiPR00476. PHFRCTKINASE.
    SUPFAMiSSF53784. SSF53784. 3 hits.
    TIGRFAMsiTIGR02478. 6PF1K_euk. 1 hit.
    PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q92448-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPEPSISALS FTSFVTNDDK LFEETFNFYT KLGFHATRSY VKDNRSDFEL    50
    TGISTDSIKE IWLESFPLSE VVETSAGREL RKPLQESVGY QSEALLGYSP 100
    YQSDGVVIKL RLSNHDLQKN KDLPGEVTFF TASIDKLRAK LIEIGAEIIP 150
    SEIDLVEFST KDPMGDVISF SSYPSLSSKK ITSPDFFLHP KKEVRSQESI 200
    VEQVKSEEGK KKIAIITSGG DAPGMNAAVR AVTRAGIFYG CKVYACYEGY 250
    TGLVKGGDML KELQWQDVRG LLSIGGTIIG TARSKEFRER WGRLQACYNM 300
    VSNGIDALVV CGGDGSLTGA DLFRNEWPEL IKELLGEGKI TKEQYETHRN 350
    LTIVGLVGSI DNDMCGTDST IGAYSSLERI IELVDYIDAT AASHSRAFVV 400
    EVMGRHCGWL GLMSGIATGA DYIFIPERPP SETNWKDDLK KVCLRHREKG 450
    RRKTTVIVAE GAIDDQLNPI TSEEVKDVLV EIGLDTRITR LGHVQRGGAP 500
    CAFDRFLATV QGVDAVRAVL ESTPAIPSPV ISILENKIVR QPLVESVAQT 550
    KTVSDAIEAK DFDKALKLRD QEFATSYESF LSVSKYDDGS YLVPESSRLN 600
    IAIIHVGAPT SALNPATRVA TLNSLAKGHR VFAIRNGFAG LIRHGAVREL 650
    NWIDVEDWHN TGGSEIGTNR SLPSDDMGTV AYYFQQYKFD GLIIIGGFEA 700
    FTALYQLDAA RAQYPIFNIP MCCLPATVSN NVPGTEYSLG SDTCLNTLSG 750
    YCDAVKQSAS ASRRRTFVVE VQGGYSGYLA SYAGLITGAL AVYTPENPIN 800
    LQTVQEDIEL LTRTYEEDDG KNRSGKIFIH NEKASKVYTT DLIAAIIGEA 850
    GKGRFESRTA VPGHVQQGKS PSSIDRVNAC RLAIKCCNFI EDANFQVKHN 900
    ANLSADERHL RFFYDDGVKT SAVSGKSSVI DDNTSVVIGI QGSEVTFTPV 950
    KQLWEKETHH KWRKGKNVHW EQLNIVSDLL SGRLSIRTT 989
    Length:989
    Mass (Da):108,817
    Last modified:July 9, 2014 - v2
    Checksum:i6708A34ED94C9DD4
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti178 – 1781S → SK in AAB97871. (PubMed:9296392)Curated
    Sequence conflicti570 – 5701D → E in AAB97871. (PubMed:9296392)Curated
    Sequence conflicti914 – 9141Y → C in AAB97871. (PubMed:9296392)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U73376 Genomic DNA. Translation: AAB97871.1.
    FN392320 Genomic DNA. Translation: CAY69023.1.
    RefSeqiXP_002491303.1. XM_002491258.1.

    Genome annotation databases

    EnsemblFungiiCAY69023; CAY69023; PAS_chr2-1_0402.
    GeneIDi8198870.
    KEGGippa:PAS_chr2-1_0402.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U73376 Genomic DNA. Translation: AAB97871.1 .
    FN392320 Genomic DNA. Translation: CAY69023.1 .
    RefSeqi XP_002491303.1. XM_002491258.1.

    3D structure databases

    ProteinModelPortali Q92448.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 644223.PAS_chr2-1_0402.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii CAY69023 ; CAY69023 ; PAS_chr2-1_0402 .
    GeneIDi 8198870.
    KEGGi ppa:PAS_chr2-1_0402.

    Phylogenomic databases

    eggNOGi COG0205.
    KOi K00850.
    OrthoDBi EOG7Q5HPV.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00182 .

    Family and domain databases

    HAMAPi MF_03184. Phosphofructokinase_I_E.
    InterProi IPR009161. 6-phosphofructokinase_euk.
    IPR022953. Phosphofructokinase.
    IPR015912. Phosphofructokinase_CS.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view ]
    Pfami PF00365. PFK. 2 hits.
    [Graphical view ]
    PIRSFi PIRSF000533. ATP_PFK_euk. 1 hit.
    PRINTSi PR00476. PHFRCTKINASE.
    SUPFAMi SSF53784. SSF53784. 3 hits.
    TIGRFAMsi TIGR02478. 6PF1K_euk. 1 hit.
    PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Glucose-induced microautophagy in Pichia pastoris requires the alpha-subunit of phosphofructokinase."
      Yuan W., Tuttle D.L., Shi Y.J., Ralph G.S., Dunn W.A. Jr.
      J. Cell Sci. 110:1935-1945(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, MUTAGENESIS OF ASP-361.
      Strain: GS115 / ATCC 20864.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: GS115 / ATCC 20864.

    Entry informationi

    Entry nameiPFKA1_PICPG
    AccessioniPrimary (citable) accession number: Q92448
    Secondary accession number(s): C4R0J9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: July 9, 2014
    Last modified: October 1, 2014
    This is version 77 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzyme, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3