Reviewed, UniProtKB/Swiss-Prot Q92444 (CHS2_PARBR)

Names and origin

Protein names

Recommended name:
    Chitin synthase 2
    EC=2.4.1.16
Alternative name(s):
    Chitin-UDP acetyl-glucosaminyl transferase 2
    Class-II chitin synthase 2

Gene names

Name:

CHS2

Organism

Paracoccidioides brasiliensis

Taxonomic identifier

121759 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Onygenales › mitosporic Onygenales › Paracoccidioides

Protein attributes

Sequence length	1043 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Plays a major role in cell wall biogenesis.
Catalytic activity	UDP-N-acetyl-D-glucosamine + (1,4-(N-acetyl-beta-D-glucosaminyl))(n) = UDP + (1,4-(N-acetyl-beta-D-glucosaminyl))(n+1).
Subcellular location	Cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the chitin synthase family. Class II subfamily.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	Glycosyltransferase Transferase
Gene Ontology (GO)
Biological process	cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW chitin biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	chitin synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1043	1043	Chitin synthase 2		PRO_0000193705
Regions
	Transmembrane	663 – 683	21	Potential
	Transmembrane	703 – 723	21	Potential
	Transmembrane	738 – 758	21	Potential
	Transmembrane	780 – 800	21	Potential
	Transmembrane	907 – 927	21	Potential
	Transmembrane	931 – 951	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q92444-1 [UniParc]. Last modified July 1, 1997. Version 2. Checksum: F63410221B325B73	FASTA	1,043	116,587
	10 20 30 40 50 60 MAQESSNMDQ SKSDNVTDNK PNRSLISMAR PSPQRCHPLD QQSASTSSLP TSRPSSSPGQ 70 80 90 100 110 120 SPNITPSILT SDTLHLPARS VSPTRPWTPS RGSEWSRHMP LSVSSVNYEP PEINCSPRPG 130 140 150 160 170 180 TPSSKYGGSP RRPLRQPVVC WPPASSAGET SIDIADGGDD SEDPFVVGER TVHQRSNTRS 190 200 210 220 230 240 SIKLIQFTMQ LSPQLLQMKR TPWVKLTLDD GEDDESDFNV HYGPAPTGRQ ERRGVRKAQM 250 260 270 280 290 300 TKKEVRLMNG ELVLECKIPR MLHSFLPRRD DREFTHMRYT AVTCDPDDFT VKGFKLRQNI 310 320 330 340 350 360 GSTMRETELF VCVTMYNENE IDFTRTMHGI MRNISHFCSR TKSRTWGKDG WQKIVVCIIA 370 380 390 400 410 420 DGRQKVHPRT LNALAAMGVY QDGIAKNVVN QKPVNAHVYE YTTQVSLDPD LKFKGAEKGI 430 440 450 460 470 480 MPCQVIFCMK ERNEKKLNSH RWFFNAFGRA LNPNICILLD VGTKPEPTAL YHLWKAFDQD 490 500 510 520 530 540 SNVAGAAGEI KAGKGKGCLG LFNPLVASQN FEYKMSNILD KPLESVFGYI TVLPGALSAY 550 560 570 580 590 600 RYHALQNDST GHGPLSMYFK GEMLHGKNAD VFTANMYLAE DRILCWELVA KREEQWVLKF 610 620 630 640 650 660 VKSAVGETDV PDTVPEFISQ RRRWLNGAFF AAVYSLIHFR QIWRTDHTIT RKILLHIEFL 670 680 690 700 710 720 YQFVSLAFTF FSLANFYLTF YFIAGALSDP TVDPFGHNIG KYIFAILRYT CVLLICLQFV 730 740 750 760 770 780 LSMGNRPQGA KKMFLSGMII YCIIMMYTVF SALYMVVMQL KTSKEMIKDS LSLGNNTFTY 790 800 810 820 830 840 IIVSTLSTVG LYFFMSFLYL DPWHMFTSSI QYFALLPSYI CRLQIYAFCN THDVTWGTKG 850 860 870 880 890 900 DNVIRTDLGT ARITSSSTVE LEMPSEQLDI DSGYDESSAI SATDSKCPRQ SHPRRRCRKT 910 920 930 940 950 960 TTAVRTYMVS VWFIANAILA MAVSEAFTEK SVGNNAYLAF VLWSVASLAV FRAVGSTAFA 970 980 990 1000 1010 1020 ILNVVHRVME GKMKFAAAGG TGYGYGSYVG SSSGGGGGSS GVRSSGAGSS LGLSSGMGEK 1030 1040 VSDWASETGW AVKRTAGKLR FWR

References

[1]

"Molecular cloning and sequencing of a chitin synthase gene (CHS2) of Paracoccidioides brasiliensis." Nino-Vega G.A., Buurman E.T., Gooday G.W., San-Blas G., Gow N.A.R. Yeast 14:181-187(1998) [PubMed: 9483806] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 32071 / IVIC Pb73 / C81.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Y09231 Genomic DNA. Translation: CAA70433.1.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT2. Glycosyltransferase Family 2.
Enzyme and pathway databases
BRENDA	2.4.1.16. 274879.
Family and domain databases
InterPro	IPR004834. Chitin_synth. IPR004835. Chitin_synth_fng. IPR013616. Chitin_synth_N. [Graphical view]
Pfam	PF01644. Chitin_synth_1. 1 hit. PF08407. Chitin_synth_1N. 1 hit. PF03142. Chitin_synth_2. 1 hit. [Graphical view]
ProDom	PD002998. Chitin_synth. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

CHS2_PARBR

Accession

Primary (citable) accession number: Q92444

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	July 1, 1997
Last modified:	October 13, 2009
This is version 52 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)