Reviewed, UniProtKB/Swiss-Prot Q92441 (MET17_KLULA)

Names and origin

Protein names

Recommended name:
    Protein MET17
Including the following 2 domains:
    1- Recommended name:
            O-acetylhomoserine sulfhydrylase
                Short name=OAH sulfhydrylase
              EC=2.5.1.49
        Alternative name(s):
            Homocysteine synthase
    2- Recommended name:
            O-acetylserine sulfhydrylase
                Short name=OAS sulfhydrylase
              EC=2.5.1.47

Gene names

Name:	MET17
Ordered Locus Names:	KLLA0D04037g

Organism

Kluyveromyces lactis (Yeast) (Candida sphaerica) [Complete proteome]

Taxonomic identifier

28985 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Kluyveromyces

Protein attributes

Sequence length	444 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Transforms O-acetylhomoserine into homocysteine and O-acetylserine into cysteine.
Catalytic activity	O-acetyl-L-homoserine + methanethiol = L-methionine + acetate. O(3)-acetyl-L-serine + H2S = L-cysteine + acetate.
Cofactor	Pyridoxal phosphate.
Pathway	Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-serine: step 2/2.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the trans-sulfuration enzymes family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Cysteine biosynthesis Methionine biosynthesis
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	cysteine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	O-acetylhomoserine aminocarboxypropyltransferase activity Inferred from electronic annotation. Source: EC cysteine synthase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 444	443	Protein MET17		PRO_0000114775
Amino acid modifications
	Modified residue	208	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q92441-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: C1183F66411BF79A	FASTA	444	48,106
	10 20 30 40 50 60 MPSHFDTLQL HAGQEKTADA HNPRAVPIYA TTSYVFNDSK HGAQLFGLET PGYIYSRIMN 70 80 90 100 110 120 PTLDVLEKRL AALEGGIAAL ATSSGQAAQT LAVTGLAHTG DNIVSTSFLY GGTYNQFKVA 130 140 150 160 170 180 FKRLGIEARF VDGDKPEDFE KLFDEKTKAL YLESIGNPKY NVPDFEKIVA VAHKHGIPVV 190 200 210 220 230 240 VDNTFGAGGF FCQPIKYGAD IVTHSATKWI GGHGVTVGGV IIDSGKFPWK DYPEKFPQFS 250 260 270 280 290 300 QPSEGYHGLI FNDAFGPAAF IGHVRTELLR DLGPVLSPFA GFLLLQGLET LSLRGERHGS 310 320 330 340 350 360 NALKLAQYLE SSPYVSWVSY PGLPSHSHHE NAKKYLENGF GGVLSFGVKD LPNASEESDP 370 380 390 400 410 420 FKASGAQVVD NLKLASNLAN VGDSKTLVIA PYFTTHQQLT DEEKLASGVT KDLIRVSVGT 430 440 EFIDDIIADF EASFATVFNG QKPE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of the Kluyveromyces lactis homocysteine synthase gene." Brzywczy J., Paszewski A. Yeast 15:1403-1409(1999) [PubMed: 10509022] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: WM27 / NRRL Y-17066.
[2]	"Genome evolution in yeasts." Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S. , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L. Nature 430:35-44(2004) [PubMed: 15229592] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NRRL Y-1140 / WM37.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U72486 Genomic DNA. Translation: AAB17387.1. CR382124 Genomic DNA. Translation: CAH00339.1.
RefSeq	XP_453243.1.
3D structure databases
HSSP	HSSP built from PDB template 1GC0 based on UniProtKB P13254.
ModBase	Search...
Protein-protein interaction databases
STRING	Q92441.
Genome annotation databases
GeneID	2892931.
GenomeReviews	Gene locus KLLA0D04037g in contig CR382124_GR.
KEGG	kla:KLLA0D04037g.
Phylogenomic databases
HOGENOM	Q92441.
OMA	SEPRPEY.
Enzyme and pathway databases
BRENDA	2.5.1.47. 74088. 2.5.1.49. 74088.
Family and domain databases
InterPro	IPR000277. Cys/Met-Metab_PyrdxlP-dep_enz. IPR006235. OAc-hSer/O-AcSer_sulfhydrylase. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit. G3DSA:3.90.1150.10. PyrdxlP-dep_Trfase_major_sub2. 1 hit.
PANTHER	PTHR11808. Cys_Met_Meta_PP. 1 hit. PTHR11808:SF12. OAH_OAS_sulfhy. 1 hit.
Pfam	PF01053. Cys_Met_Meta_PP. 1 hit. [Graphical view]
PIRSF	PIRSF001434. CGS. 1 hit.
TIGRFAMs	TIGR01326. OAH_OAS_sulfhy. 1 hit.
PROSITE	PS00868. CYS_MET_METAB_PP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MET17_KLULA

Accession

Primary (citable) accession number: Q92441

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	January 23, 2007
Last modified:	November 3, 2009
This is version 68 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)