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Protein

Multifunctional tryptophan biosynthesis protein

Gene

TRP1

Organism
Cochliobolus heterostrophus (Southern corn leaf blight fungus) (Bipolaris maydis)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Trifunctional enzyme bearing the Gln amidotransferase (GATase) domain of anthranilate synthase, indole-glycerolphosphate synthase, and phosphoribosylanthranilate isomerase activities.

Catalytic activityi

N-(5-phospho-beta-D-ribosyl)anthranilate = 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate.
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate = 1-C-(3-indolyl)-glycerol 3-phosphate + CO2 + H2O.
Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate.

Pathway: L-tryptophan biosynthesis

This protein is involved in step 1, 3 and 4 of the subpathway that synthesizes L-tryptophan from chorismate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Multifunctional tryptophan biosynthesis protein (TRP1)
  2. no protein annotated in this organism
  3. Multifunctional tryptophan biosynthesis protein (TRP1)
  4. Multifunctional tryptophan biosynthesis protein (TRP1)
  5. no protein annotated in this organism
This subpathway is part of the pathway L-tryptophan biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-tryptophan from chorismate, the pathway L-tryptophan biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei103 – 1031For GATase activityBy similarity
Active sitei199 – 1991For GATase activityBy similarity
Active sitei201 – 2011For GATase activityBy similarity

GO - Molecular functioni

GO - Biological processi

Keywords - Molecular functioni

Decarboxylase, Isomerase, Lyase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis, Tryptophan biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00035; UER00040.
UPA00035; UER00042.
UPA00035; UER00043.

Names & Taxonomyi

Protein namesi
Recommended name:
Multifunctional tryptophan biosynthesis protein
Including the following 3 domains:
Anthranilate synthase component 2 (EC:4.1.3.27)
Short name:
AS
Alternative name(s):
Anthranilate synthase, glutamine amidotransferase component
Indole-3-glycerol phosphate synthase (EC:4.1.1.48)
Short name:
IGPS
N-(5'-phosphoribosyl)anthranilate isomerase (EC:5.3.1.24)
Short name:
PRAI
Gene namesi
Name:TRP1
OrganismiCochliobolus heterostrophus (Southern corn leaf blight fungus) (Bipolaris maydis)
Taxonomic identifieri5016 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaDothideomycetesPleosporomycetidaePleosporalesPleosporineaePleosporaceaeBipolaris

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 768768Multifunctional tryptophan biosynthesis proteinPRO_0000056856Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ92411.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini25 – 225201Glutamine amidotransferase type-1Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni256 – 520265Indole-3-glycerol phosphate synthaseAdd
BLAST
Regioni535 – 768234N-(5'-phosphoribosyl)anthranilate isomeraseAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Glutamine amidotransferase

Family and domain databases

Gene3Di3.20.20.70. 3 hits.
3.40.50.880. 1 hit.
HAMAPiMF_00135. PRAI.
InterProiIPR013785. Aldolase_TIM.
IPR016302. Anthranilate_synth_II.
IPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR013798. Indole-3-glycerol_P_synth.
IPR001468. Indole-3-GlycerolPSynthase_CS.
IPR001240. PRAI.
IPR011060. RibuloseP-bd_barrel.
IPR006221. TrpG/PapA_dom.
[Graphical view]
PfamiPF00117. GATase. 1 hit.
PF00218. IGPS. 1 hit.
PF00697. PRAI. 1 hit.
[Graphical view]
PIRSFiPIRSF001382. TrpG-trpC-trpF. 1 hit.
SUPFAMiSSF51366. SSF51366. 3 hits.
SSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR00566. trpG_papA. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
PS00614. IGPS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q92411-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPSSTLIDHS PRNPNPSPPR ITASNVILID NYDSFTWNVY QYLVFEGAFV
60 70 80 90 100
TVYRNDEITL DELIAKKPTQ LVISQVPDTR KEDVALAIEP LRTSVAKSPF
110 120 130 140 150
SACAWESCIF YSYGGTVDVT GQILHGKTCP LKHDGKGVFA GVPQDVPVTR
160 170 180 190 200
YHSLAGTHGT LPDCLEISAT IPANADAEIK EVIMGVRHKE YVIEGVQFHP
210 220 230 240 250
ESILTEHGRL MMRNFLKMQG GTWAENERLQ KEAHAQAIGA AFSESAMVQR
260 270 280 290 300
TDSRTHLQKI YDHRRAAVAE QKKIPSNRPV DLQAAYDSNL APPQINFPER
310 320 330 340 350
LRQSPFKLSL MSEIKRASPS KGIISLSTCA PAQARLYAKA GARPISVLTE
360 370 380 390 400
PEWFKGSIED LRAVRQSIEG MANRPALLRK EFIFDEYQIL EGRLAGADTV
410 420 430 440 450
LVIVKMLETE LLERLLGLSD SVGMEPLVEV QNTDEMKIAV KLGAQVIGVN
460 470 480 490 500
NRNLVNFEVD IGTTNRLIQM VPKETILCAL SGIAGPKDVE AYIKDGVGAV
510 520 530 540 550
LVGEALMRAS DMSQFISELL GGPYKEVRAS SSSPIVKISG TRSEAAVAAV
560 570 580 590 600
EAGADLIGII LAPGLKRTVT AETALSISEA VHKTKKPNVS KSGLLADSKT
610 620 630 640 650
ASDFFDHGAS RLLETISRAL LVGVFRNQSL EYVLEQQRLL NLDVVQLHGQ
660 670 680 690 700
EPIEWAKLVP VPVLKAFNPQ DHGIGTRGYH ALPLLDAGSG GSGKQLGLSD
710 720 730 740 750
VKAAFTKDDG IKIILGRGLE PDNVQTTLAG LDEYRDRVYA VDVSSGVEED
760
GQQSLDKIRS FVKAAKSL
Length:768
Mass (Da):83,655
Last modified:February 1, 1997 - v1
Checksum:iB2F215AA304B8B00
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X70035 Genomic DNA. Translation: CAA49629.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X70035 Genomic DNA. Translation: CAA49629.1.

3D structure databases

ProteinModelPortaliQ92411.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00035; UER00040.
UPA00035; UER00042.
UPA00035; UER00043.

Family and domain databases

Gene3Di3.20.20.70. 3 hits.
3.40.50.880. 1 hit.
HAMAPiMF_00135. PRAI.
InterProiIPR013785. Aldolase_TIM.
IPR016302. Anthranilate_synth_II.
IPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR013798. Indole-3-glycerol_P_synth.
IPR001468. Indole-3-GlycerolPSynthase_CS.
IPR001240. PRAI.
IPR011060. RibuloseP-bd_barrel.
IPR006221. TrpG/PapA_dom.
[Graphical view]
PfamiPF00117. GATase. 1 hit.
PF00218. IGPS. 1 hit.
PF00697. PRAI. 1 hit.
[Graphical view]
PIRSFiPIRSF001382. TrpG-trpC-trpF. 1 hit.
SUPFAMiSSF51366. SSF51366. 3 hits.
SSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR00566. trpG_papA. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
PS00614. IGPS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complementation of Cochliobolus heterostrophus trp-mutants produced by gene replacement."
    Mullin P.G., Turgeon B.G., Yoder O.C.
    Fungal Genet. Newsl. 40:51-53(1993)
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 48330 / C3.

Entry informationi

Entry nameiTRPG_COCHE
AccessioniPrimary (citable) accession number: Q92411
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: April 1, 2015
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Multifunctional enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.