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Q92407 (HXKG_ASPNG) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glucokinase

EC=2.7.1.2
Alternative name(s):
Glucose kinase
Short name=GLK
Gene names
Name:glkA
OrganismAspergillus niger
Taxonomic identifier5061 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus

Protein attributes

Sequence length495 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

The enzyme has great affinity for glucose. Mannose, 2-deoxyglucose and glucosamine can serve as substrates.

Catalytic activity

ATP + D-glucose = ADP + D-glucose 6-phosphate.

Subunit structure

Monomer Probable.

Sequence similarities

Belongs to the hexokinase family.

Contains 1 hexokinase type-1 domain.

Contains 1 hexokinase type-2 domain.

Biophysicochemical properties

pH dependence:

Activity is relatively constant from pH 7.5 to 9.0. Below pH 7.5, activity decreases with pH.

Ontologies

Keywords
   Biological processGlycolysis
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
Gene Ontology (GO)
   Biological_processglycolysis

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

glucokinase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 495495Glucokinase
PRO_0000197611

Regions

Domain8 – 220213Hexokinase type-1
Domain226 – 487262Hexokinase type-2
Nucleotide binding472 – 4776ATP Potential
Region149 – 17527Glucose-binding Potential

Sites

Binding site931ATP Potential

Sequences

Sequence LengthMass (Da)Tools
Q92407 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: 02C94EF07D1809F8

FASTA49554,537
        10         20         30         40         50         60 
MSSALLDEAA RIARQFDYPA AEVQRGVTEY IREIDEGLSK EHTTLSQIPT YVTAVPNGTE 

        70         80         90        100        110        120 
KGLYLAVDLG GTNFRVCSID LHGDTTFSLT QSKIMIPRET MASGTAKDLF LFLARQIESF 

       130        140        150        160        170        180 
LRIHHNDHFE AHLRRRNEKN GNCEEDLFDL GFTFSFPVRQ LGINKGTLIR WTKGFNIPDA 

       190        200        210        220        230        240 
VGKDVCALLQ NAIDDLGLPV RVAALVNDTV GTLMARSYTS PGETGTFLGA IFGTGTNGAY 

       250        260        270        280        290        300 
VEKLDRITKL QTIEHSEYDK TTGEMIINAE WGSFDNHLSV LPNTIYDQQL DADSNNPGIQ 

       310        320        330        340        350        360 
MFEKRVSGMF LGEILRRVML DMQRNESLGF LRAGGASTVS VPQESSLYRQ WGIDTSLLSL 

       370        380        390        400        410        420 
VEADKTENME QIKVALKDHL KIERPTTDDC KAIQTVVHAI GKRAARLSAV PLAAILLSTG 

       430        440        450        460        470        480 
KLQKDDLVDI GVDGSLVEFY PNFEGYMRDA LREVPEVGEA GNKKIRIGIS KDGSGVGAAL 

       490 
IALVASKEET RRKSQ 

« Hide

References

[1]"Cloning and biochemical characterisation of an Aspergillus niger glucokinase. Evidence for the presence of separate glucokinase and hexokinase enzymes."
Panneman H., Ruijter G.J.G., van den Broeck H.C., Driever E.T.M., Visser J.
Eur. J. Biochem. 240:518-525(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 9029 / NRRL 3 / CBS 120.49 / DSM 2466 / N400.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X99626 Genomic DNA. Translation: CAA67949.1.
PIRS74210.

3D structure databases

ProteinModelPortalQ92407.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEQ92407.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

eggNOGCOG5026.

Family and domain databases

InterProIPR001312. Hexokinase.
IPR022673. Hexokinase_C.
IPR019807. Hexokinase_CS.
IPR022672. Hexokinase_N.
[Graphical view]
PANTHERPTHR19443. PTHR19443. 1 hit.
PfamPF00349. Hexokinase_1. 1 hit.
PF03727. Hexokinase_2. 1 hit.
[Graphical view]
PRINTSPR00475. HEXOKINASE.
PROSITEPS00378. HEXOKINASES. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameHXKG_ASPNG
AccessionPrimary (citable) accession number: Q92407
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: April 16, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families