Q92407 (HXKG_ASPNG) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glucokinase
EC=2.7.1.2

Alternative name(s):
Glucose kinase
Short name=GLK

Gene names

Name:

glkA

Organism

Aspergillus niger

Taxonomic identifier

5061 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus

Protein attributes

Sequence length	495 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The enzyme has great affinity for glucose. Mannose, 2-deoxyglucose and glucosamine can serve as substrates.
Catalytic activity	ATP + D-glucose = ADP + D-glucose 6-phosphate.
Subunit structure	Monomer Probable.
Sequence similarities	Belongs to the hexokinase family.
Biophysicochemical properties	pH dependence: Activity is relatively constant from pH 7.5 to 9.0. Below pH 7.5, activity decreases with pH.

Ontologies

Keywords
Biological process	Glycolysis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Gene Ontology (GO)
Biological_process	glucose 6-phosphate metabolic process Inferred from electronic annotation. Source: GOC glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glucokinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 495	495	Glucokinase		PRO_0000197611
Regions
	Nucleotide binding	472 – 477	6	ATP Potential
	Region	149 – 175	27	Glucose-binding Potential
Sites
	Binding site	93	1	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q92407 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 02C94EF07D1809F8	FASTA	495	54,537
	10 20 30 40 50 60 MSSALLDEAA RIARQFDYPA AEVQRGVTEY IREIDEGLSK EHTTLSQIPT YVTAVPNGTE 70 80 90 100 110 120 KGLYLAVDLG GTNFRVCSID LHGDTTFSLT QSKIMIPRET MASGTAKDLF LFLARQIESF 130 140 150 160 170 180 LRIHHNDHFE AHLRRRNEKN GNCEEDLFDL GFTFSFPVRQ LGINKGTLIR WTKGFNIPDA 190 200 210 220 230 240 VGKDVCALLQ NAIDDLGLPV RVAALVNDTV GTLMARSYTS PGETGTFLGA IFGTGTNGAY 250 260 270 280 290 300 VEKLDRITKL QTIEHSEYDK TTGEMIINAE WGSFDNHLSV LPNTIYDQQL DADSNNPGIQ 310 320 330 340 350 360 MFEKRVSGMF LGEILRRVML DMQRNESLGF LRAGGASTVS VPQESSLYRQ WGIDTSLLSL 370 380 390 400 410 420 VEADKTENME QIKVALKDHL KIERPTTDDC KAIQTVVHAI GKRAARLSAV PLAAILLSTG 430 440 450 460 470 480 KLQKDDLVDI GVDGSLVEFY PNFEGYMRDA LREVPEVGEA GNKKIRIGIS KDGSGVGAAL 490 IALVASKEET RRKSQ

References

[1]

"Cloning and biochemical characterisation of an Aspergillus niger glucokinase. Evidence for the presence of separate glucokinase and hexokinase enzymes." Panneman H., Ruijter G.J.G., van den Broeck H.C., Driever E.T.M., Visser J. Eur. J. Biochem. 240:518-525(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 9029 / NRRL 3 / CBS 120.49 / DSM 2466 / N400.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X99626 Genomic DNA. Translation: CAA67949.1.
PIR	S74210.
3D structure databases
ProteinModelPortal	Q92407.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
eggNOG	COG5026.
Family and domain databases
InterPro	IPR001312. Hexokinase. IPR022673. Hexokinase_C. IPR019807. Hexokinase_CS. IPR022672. Hexokinase_N. [Graphical view]
PANTHER	PTHR19443. PTHR19443. 1 hit.
Pfam	PF00349. Hexokinase_1. 1 hit. PF03727. Hexokinase_2. 1 hit. [Graphical view]
PRINTS	PR00475. HEXOKINASE.
PROSITE	PS00378. HEXOKINASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HXKG_ASPNG

Accession

Primary (citable) accession number: Q92407

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	April 3, 2013
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program