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Protein

Glucokinase

Gene

glkA

Organism
Aspergillus niger
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

The enzyme has great affinity for glucose. Mannose, 2-deoxyglucose and glucosamine can serve as substrates.

Catalytic activityi

ATP + D-glucose = ADP + D-glucose 6-phosphate.

pH dependencei

Activity is relatively constant from pH 7.5 to 9.0. Below pH 7.5, activity decreases with pH.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei93 – 931ATPSequence Analysis

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi472 – 4776ATPSequence Analysis

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. glucokinase activity Source: UniProtKB-EC

GO - Biological processi

  1. glycolytic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.1.2. 518.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucokinase (EC:2.7.1.2)
Alternative name(s):
Glucose kinase
Short name:
GLK
Gene namesi
Name:glkA
OrganismiAspergillus niger
Taxonomic identifieri5061 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 495495GlucokinasePRO_0000197611Add
BLAST

Proteomic databases

PRIDEiQ92407.

Interactioni

Subunit structurei

Monomer.Curated

Structurei

3D structure databases

ProteinModelPortaliQ92407.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 483481HexokinasePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni57 – 206150Hexokinase small subdomainPROSITE-ProRule annotationAdd
BLAST
Regioni149 – 17527Glucose-bindingSequence AnalysisAdd
BLAST
Regioni207 – 472266Hexokinase large subdomainPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the hexokinase family.PROSITE-ProRule annotationCurated
Contains 1 hexokinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5026.

Family and domain databases

InterProiIPR001312. Hexokinase.
IPR022673. Hexokinase_C.
IPR019807. Hexokinase_CS.
IPR022672. Hexokinase_N.
[Graphical view]
PANTHERiPTHR19443. PTHR19443. 1 hit.
PfamiPF00349. Hexokinase_1. 1 hit.
PF03727. Hexokinase_2. 1 hit.
[Graphical view]
PRINTSiPR00475. HEXOKINASE.
PROSITEiPS00378. HEXOKINASE_1. 1 hit.
PS51748. HEXOKINASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q92407-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSALLDEAA RIARQFDYPA AEVQRGVTEY IREIDEGLSK EHTTLSQIPT
60 70 80 90 100
YVTAVPNGTE KGLYLAVDLG GTNFRVCSID LHGDTTFSLT QSKIMIPRET
110 120 130 140 150
MASGTAKDLF LFLARQIESF LRIHHNDHFE AHLRRRNEKN GNCEEDLFDL
160 170 180 190 200
GFTFSFPVRQ LGINKGTLIR WTKGFNIPDA VGKDVCALLQ NAIDDLGLPV
210 220 230 240 250
RVAALVNDTV GTLMARSYTS PGETGTFLGA IFGTGTNGAY VEKLDRITKL
260 270 280 290 300
QTIEHSEYDK TTGEMIINAE WGSFDNHLSV LPNTIYDQQL DADSNNPGIQ
310 320 330 340 350
MFEKRVSGMF LGEILRRVML DMQRNESLGF LRAGGASTVS VPQESSLYRQ
360 370 380 390 400
WGIDTSLLSL VEADKTENME QIKVALKDHL KIERPTTDDC KAIQTVVHAI
410 420 430 440 450
GKRAARLSAV PLAAILLSTG KLQKDDLVDI GVDGSLVEFY PNFEGYMRDA
460 470 480 490
LREVPEVGEA GNKKIRIGIS KDGSGVGAAL IALVASKEET RRKSQ
Length:495
Mass (Da):54,537
Last modified:January 31, 1997 - v1
Checksum:i02C94EF07D1809F8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X99626 Genomic DNA. Translation: CAA67949.1.
PIRiS74210.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X99626 Genomic DNA. Translation: CAA67949.1.
PIRiS74210.

3D structure databases

ProteinModelPortaliQ92407.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ92407.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiCOG5026.

Enzyme and pathway databases

BRENDAi2.7.1.2. 518.

Family and domain databases

InterProiIPR001312. Hexokinase.
IPR022673. Hexokinase_C.
IPR019807. Hexokinase_CS.
IPR022672. Hexokinase_N.
[Graphical view]
PANTHERiPTHR19443. PTHR19443. 1 hit.
PfamiPF00349. Hexokinase_1. 1 hit.
PF03727. Hexokinase_2. 1 hit.
[Graphical view]
PRINTSiPR00475. HEXOKINASE.
PROSITEiPS00378. HEXOKINASE_1. 1 hit.
PS51748. HEXOKINASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and biochemical characterisation of an Aspergillus niger glucokinase. Evidence for the presence of separate glucokinase and hexokinase enzymes."
    Panneman H., Ruijter G.J.G., van den Broeck H.C., Driever E.T.M., Visser J.
    Eur. J. Biochem. 240:518-525(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 9029 / NRRL 3 / CBS 120.49 / DSM 2466 / N400.

Entry informationi

Entry nameiHXKG_ASPNG
AccessioniPrimary (citable) accession number: Q92407
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 1997
Last sequence update: January 31, 1997
Last modified: March 31, 2015
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.