Q92406 (NDUV1_ASPNG) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADH-ubiquinone oxidoreductase 51 kDa subunit, mitochondrial
EC=1.6.5.3
EC=1.6.99.3

Alternative name(s):
Complex I-51kD
Short name=CI-51kD

Gene names

Name:

NUO51

Organism

Aspergillus niger

Taxonomic identifier

5061 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus

Protein attributes

Sequence length	496 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone By similarity.
Catalytic activity	NADH + ubiquinone = NAD+ + ubiquinol. NADH + acceptor = NAD+ + reduced acceptor.
Cofactor	Binds 1 FMN Potential. Binds 1 4Fe-4S cluster Potential.
Subunit structure	Complex I is composed of about 40 different subunits. This is a component of the flavoprotein-sulfur (FP) fragment of the enzyme By similarity.
Subcellular location	Mitochondrion inner membrane; Peripheral membrane protein; Matrix side.
Sequence similarities	Belongs to the complex I 51 kDa subunit family.

Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transit peptide
Ligand	4Fe-4S FMN Flavoprotein Iron Iron-sulfur Metal-binding NAD Ubiquinone
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological_process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-SubCell respiratory chain Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW FMN binding Inferred from electronic annotation. Source: InterPro NAD binding Inferred from electronic annotation. Source: InterPro NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 30	30	Mitochondrion By similarity
	Chain	31 – 496	466	NADH-ubiquinone oxidoreductase 51 kDa subunit, mitochondrial		PRO_0000019979
Regions
	Nucleotide binding	98 – 107	10	NAD By similarity
	Nucleotide binding	214 – 261	48	FMN By similarity
Sites
	Metal binding	393	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	396	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	399	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	439	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q92406 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: DE64F00A8E32B42A	FASTA	496	54,452
	10 20 30 40 50 60 MISRAAAPSS SIASLSSRSL RAQAPAARSF ATVQDNAPPV KHYGGLKDQD RIFTNLYGHH 70 80 90 100 110 120 GADLKSAMKY GDWHRTKDIV LKGHDWLISE LKASGLRGRG GAGFPSGLKY SFMNFKDWDK 130 140 150 160 170 180 DPRPRYLVVN ADEGEPGTCK DREIMRKDPQ KLIEGCLVVG RAMNANAAYM YIRGEFYQEA 190 200 210 220 230 240 TVLQRAINEA YEAGLIGKNA CGTGYDFDVY IHRGMGAYVC GEETSLIESI EGKAGKPRLK 250 260 270 280 290 300 PPFPAAVGLF GCPSTVTNVE TVAVTPTIMR RGASWFSSFG RERNAGTKLF CISGHVNNPC 310 320 330 340 350 360 TVEEEMSISL RDVIDRHCGG VRGGWDNLLA VIPGGSSTPV LPKTICDDQL MDFDALKDSQ 370 380 390 400 410 420 SGLGTAAVIV MDKSTDIVRA ISRLSTFYKH ESCGQCTPCR EGSKWTMHMM QRMEKGQARE 430 440 450 460 470 480 REIDMLQELT KQVEGHTICA LGEAFAWPIQ GLIRHFRPEL EARIREYSKE VGGNGPYAGG 490 WHPEARAEGK LISPGM

References

[1]

"The role of the proton-pumping and alternative respiratory chain NADH:ubiquinone oxidoreductases in overflow catabolism of Aspergillus niger." Proemper C., Schneider R., Weiss H. Eur. J. Biochem. 216:223-230(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: FGSC 732.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X64402 Genomic DNA. Translation: CAA45744.1.
3D structure databases
ProteinModelPortal	Q92406.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
eggNOG	COG1894.
Family and domain databases
InterPro	IPR001949. NADH-UbQ_OxRdtase_51kDa_CS. IPR019575. NADH-UbQ_OxRdtase_Fsu_4Fe4S-bd. IPR011537. NADH-UbQ_OxRdtase_suF. IPR011538. NADH_UbQ_OxRdtase_51kDa_su. IPR019554. Soluble_ligand-bd. [Graphical view]
Pfam	PF01512. Complex1_51K. 1 hit. PF10589. NADH_4Fe-4S. 1 hit. PF10531. SLBB. 1 hit. [Graphical view]
SMART	SM00928. NADH_4Fe-4S. 1 hit. [Graphical view]
TIGRFAMs	TIGR01959. nuoF_fam. 1 hit.
PROSITE	PS00644. COMPLEX1_51K_1. 1 hit. PS00645. COMPLEX1_51K_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NDUV1_ASPNG

Accession

Primary (citable) accession number: Q92406

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	April 3, 2013
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program