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Protein

NADH-ubiquinone oxidoreductase 51 kDa subunit, mitochondrial

Gene

NUO51

Organism
Aspergillus niger
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity).By similarity

Catalytic activityi

NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).
NADH + acceptor = NAD+ + reduced acceptor.

Cofactori

Protein has several cofactor binding sites:
  • FMNCuratedNote: Binds 1 FMN.Curated
  • [4Fe-4S] clusterCuratedNote: Binds 1 [4Fe-4S] cluster.Curated

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi393 – 3931Iron-sulfur (4Fe-4S)Sequence Analysis
Metal bindingi396 – 3961Iron-sulfur (4Fe-4S)Sequence Analysis
Metal bindingi399 – 3991Iron-sulfur (4Fe-4S)Sequence Analysis
Metal bindingi439 – 4391Iron-sulfur (4Fe-4S)Sequence Analysis

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi98 – 10710NADBy similarity
Nucleotide bindingi214 – 26148FMNBy similarityAdd
BLAST

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  2. FMN binding Source: InterPro
  3. metal ion binding Source: UniProtKB-KW
  4. NAD binding Source: InterPro
  5. NADH dehydrogenase (ubiquinone) activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Keywords - Ligandi

4Fe-4S, Flavoprotein, FMN, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-ubiquinone oxidoreductase 51 kDa subunit, mitochondrial (EC:1.6.5.3, EC:1.6.99.3)
Alternative name(s):
Complex I-51kD
Short name:
CI-51kD
Gene namesi
Name:NUO51
OrganismiAspergillus niger
Taxonomic identifieri5061 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus

Subcellular locationi

GO - Cellular componenti

  1. mitochondrial inner membrane Source: UniProtKB-SubCell
  2. respiratory chain Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3030MitochondrionBy similarityAdd
BLAST
Chaini31 – 496466NADH-ubiquinone oxidoreductase 51 kDa subunit, mitochondrialPRO_0000019979Add
BLAST

Interactioni

Subunit structurei

Complex I is composed of about 40 different subunits. This is a component of the flavoprotein-sulfur (FP) fragment of the enzyme (By similarity).By similarity

Family & Domainsi

Sequence similaritiesi

Belongs to the complex I 51 kDa subunit family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG1894.

Family and domain databases

InterProiIPR001949. NADH-UbQ_OxRdtase_51kDa_CS.
IPR011537. NADH-UbQ_OxRdtase_suF.
IPR011538. Nuo51_FMN-bd.
IPR019575. Nuop51_4Fe4S-bd.
IPR019554. Soluble_ligand-bd.
[Graphical view]
PfamiPF01512. Complex1_51K. 1 hit.
PF10589. NADH_4Fe-4S. 1 hit.
PF10531. SLBB. 1 hit.
[Graphical view]
SMARTiSM00928. NADH_4Fe-4S. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01959. nuoF_fam. 1 hit.
PROSITEiPS00644. COMPLEX1_51K_1. 1 hit.
PS00645. COMPLEX1_51K_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q92406-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MISRAAAPSS SIASLSSRSL RAQAPAARSF ATVQDNAPPV KHYGGLKDQD
60 70 80 90 100
RIFTNLYGHH GADLKSAMKY GDWHRTKDIV LKGHDWLISE LKASGLRGRG
110 120 130 140 150
GAGFPSGLKY SFMNFKDWDK DPRPRYLVVN ADEGEPGTCK DREIMRKDPQ
160 170 180 190 200
KLIEGCLVVG RAMNANAAYM YIRGEFYQEA TVLQRAINEA YEAGLIGKNA
210 220 230 240 250
CGTGYDFDVY IHRGMGAYVC GEETSLIESI EGKAGKPRLK PPFPAAVGLF
260 270 280 290 300
GCPSTVTNVE TVAVTPTIMR RGASWFSSFG RERNAGTKLF CISGHVNNPC
310 320 330 340 350
TVEEEMSISL RDVIDRHCGG VRGGWDNLLA VIPGGSSTPV LPKTICDDQL
360 370 380 390 400
MDFDALKDSQ SGLGTAAVIV MDKSTDIVRA ISRLSTFYKH ESCGQCTPCR
410 420 430 440 450
EGSKWTMHMM QRMEKGQARE REIDMLQELT KQVEGHTICA LGEAFAWPIQ
460 470 480 490
GLIRHFRPEL EARIREYSKE VGGNGPYAGG WHPEARAEGK LISPGM
Length:496
Mass (Da):54,452
Last modified:February 1, 1997 - v1
Checksum:iDE64F00A8E32B42A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X64402 Genomic DNA. Translation: CAA45744.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X64402 Genomic DNA. Translation: CAA45744.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiCOG1894.

Family and domain databases

InterProiIPR001949. NADH-UbQ_OxRdtase_51kDa_CS.
IPR011537. NADH-UbQ_OxRdtase_suF.
IPR011538. Nuo51_FMN-bd.
IPR019575. Nuop51_4Fe4S-bd.
IPR019554. Soluble_ligand-bd.
[Graphical view]
PfamiPF01512. Complex1_51K. 1 hit.
PF10589. NADH_4Fe-4S. 1 hit.
PF10531. SLBB. 1 hit.
[Graphical view]
SMARTiSM00928. NADH_4Fe-4S. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01959. nuoF_fam. 1 hit.
PROSITEiPS00644. COMPLEX1_51K_1. 1 hit.
PS00645. COMPLEX1_51K_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The role of the proton-pumping and alternative respiratory chain NADH:ubiquinone oxidoreductases in overflow catabolism of Aspergillus niger."
    Proemper C., Schneider R., Weiss H.
    Eur. J. Biochem. 216:223-230(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: FGSC 732.

Entry informationi

Entry nameiNDUV1_ASPNG
AccessioniPrimary (citable) accession number: Q92406
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: April 1, 2015
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.