Reviewed, UniProtKB/Swiss-Prot Q92403 (HEM1_AGABI)

Names and origin

Protein names

Recommended name:
    5-aminolevulinate synthase, mitochondrial
    EC=2.3.1.37
Alternative name(s):
    5-aminolevulinic acid synthase
    Delta-aminolevulinate synthase
    Delta-ALA synthetase

Gene names

Name:

hem1

Organism

Agaricus bisporus (Common mushroom)

Taxonomic identifier

5341 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Basidiomycota › Agaricomycotina › Homobasidiomycetes › Agaricomycetidae › Agaricales › Agaricaceae › Agaricus

Protein attributes

Sequence length	621 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2.
Cofactor	Pyridoxal phosphate.
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from glycine: step 1/1.
Subunit structure	Homodimer By similarity.
Subcellular location	Mitochondrion matrix.
Sequence similarities	Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.

Ontologies

Keywords
Biological process	Heme biosynthesis
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	Pyridoxal phosphate
Molecular function	Acyltransferase Transferase
Gene Ontology (GO)
Biological process	heme biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	5-aminolevulinate synthase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro transferase activity, transferring nitrogenous groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion
	Chain	? – 621		5-aminolevulinate synthase, mitochondrial		PRO_0000001235
Amino acid modifications
	Modified residue	362	1	N6-(pyridoxal phosphate)lysine Probable

Sequences

	Sequence		Length	Mass (Da)
	Q92403-1 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: A335C3268FAE1AA3	FASTA	621	67,426
	10 20 30 40 50 60 MSHHGPRLST RSQQIVAGYA SVAANFDVEK LHREQGVNIP TSGASINQCP HAAAARAAAR 70 80 90 100 110 120 MADDLASAAR AKKSLDAKGS LAGRPVHHKA ATESTKAKHT GFDYEAFYKG ELAKKHQDKS 130 140 150 160 170 180 YRYFNNINRL ARKFPVAHTA NPRDEVEVWC SNDYLGMGNN PVVLETMHRT LDKYGHGAGG 190 200 210 220 230 240 TRNIAGNGAM HLGLERELRL HRKEAALVFS SCYVANDATL STLGTKLPGC VIFSDTMNHA 250 260 270 280 290 300 SMIQGMRHST PKRVIFKHND LEDLETKLQQ YPKETPKIIA FESVYSMCGS IGPVKEICDL 310 320 330 340 350 360 AEQYGAITFL DEVHAVGLYG PRGAGVAEHL DYDAHLAAGS SPDPIPGSVM DRIDIITGTL 370 380 390 400 410 420 GKSYGAVGGY IAGSEEFVDM IRSYAPGFIF TTSLPPATVA GARASIVYQS EYLGDRQLKQ 430 440 450 460 470 480 INVREVKRRL AELDIPVVPG SSHIVPVLVG DAALARAASD KLLSEHDIYV QAINYPTVAR 490 500 510 520 530 540 GEERLRITVT PRHTMEQMEG LIRSLNQVFE ELNINRLSDW KLAGGRAGVG IPGAADDVQP 550 560 570 580 590 600 IWTDEQIGLL NGTAPRSLRN AEKSVVDMRA VTIARSRFDV LLGPVYGELQ PTEDFDTPAV 610 620 GATFKAPLVD REVAHDITVS A

References

[1]	Yague E., Mehak-Zunic M., Wood D.A., Thurston C.F. Submitted (NOV-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: D649.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z50096 mRNA. Translation: CAA90424.1.
3D structure databases
HSSP	HSSP built from PDB template 1FC4 based on UniProtKB P07912.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.3.1.37. 984.
Family and domain databases
InterPro	IPR010961. 4pyrrol_synth_NH2levulA_synth. IPR004839. Aminotrans_I/II. IPR001917. Aminotrans_II_pyridoxalP_BS. IPR015421. PyrdxlP-dep_Trfase_major_sub1. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
Pfam	PF00155. Aminotran_1_2. 1 hit. [Graphical view]
TIGRFAMs	TIGR01821. 5aminolev_synth. 1 hit.
PROSITE	PS00599. AA_TRANSFER_CLASS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEM1_AGABI

Accession

Primary (citable) accession number: Q92403

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	June 16, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)