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Protein

Adhesion G protein-coupled receptor L4

Gene

Adgrl4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Endothelial orphan receptor that acts as a key regulator of angiogenesis.By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Keywords - Ligandi

Calcium

Protein family/group databases

MEROPSiP02.013.

Names & Taxonomyi

Protein namesi
Recommended name:
Adhesion G protein-coupled receptor L4
Alternative name(s):
EGF, latrophilin seven transmembrane domain-containing protein 1
Gene namesi
Name:Adgrl4
Synonyms:Eltd1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:2655562. Adgrl4.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini20 – 481462ExtracellularCuratedAdd
BLAST
Transmembranei482 – 50221Helical; Name=1Sequence analysisAdd
BLAST
Topological domaini503 – 51311CytoplasmicCuratedAdd
BLAST
Transmembranei514 – 53421Helical; Name=2Sequence analysisAdd
BLAST
Topological domaini535 – 54814ExtracellularCuratedAdd
BLAST
Transmembranei549 – 56921Helical; Name=3Sequence analysisAdd
BLAST
Topological domaini570 – 58112CytoplasmicCuratedAdd
BLAST
Transmembranei582 – 60221Helical; Name=4Sequence analysisAdd
BLAST
Topological domaini603 – 62220ExtracellularCuratedAdd
BLAST
Transmembranei623 – 64321Helical; Name=5Sequence analysisAdd
BLAST
Topological domaini644 – 66724CytoplasmicCuratedAdd
BLAST
Transmembranei668 – 68821Helical; Name=6Sequence analysisAdd
BLAST
Topological domaini689 – 6957ExtracellularCurated
Transmembranei696 – 71621Helical; Name=7Sequence analysisAdd
BLAST
Topological domaini717 – 73923CytoplasmicCuratedAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

No visible phenotype but deficient mice present increased cardiac hypertrophy in response to pressure overload.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Sequence analysisAdd
BLAST
Chaini20 – 739720Adhesion G protein-coupled receptor L4PRO_0000012871Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi21 – 211N-linked (GlcNAc...)Sequence analysis
Disulfide bondi22 ↔ 33PROSITE-ProRule annotation
Disulfide bondi27 ↔ 42PROSITE-ProRule annotation
Disulfide bondi44 ↔ 56PROSITE-ProRule annotation
Disulfide bondi62 ↔ 74PROSITE-ProRule annotation
Disulfide bondi68 ↔ 83PROSITE-ProRule annotation
Disulfide bondi85 ↔ 106PROSITE-ProRule annotation
Disulfide bondi112 ↔ 124PROSITE-ProRule annotation
Disulfide bondi118 ↔ 133PROSITE-ProRule annotation
Disulfide bondi135 ↔ 156PROSITE-ProRule annotation
Glycosylationi176 – 1761N-linked (GlcNAc...)Sequence analysis
Glycosylationi226 – 2261N-linked (GlcNAc...)Sequence analysis
Glycosylationi237 – 2371N-linked (GlcNAc...)Sequence analysis
Glycosylationi298 – 2981N-linked (GlcNAc...)Sequence analysis
Glycosylationi422 – 4221N-linked (GlcNAc...)Sequence analysis
Glycosylationi430 – 4301N-linked (GlcNAc...)Sequence analysis
Glycosylationi444 – 4441N-linked (GlcNAc...)Sequence analysis

Post-translational modificationi

Glycosylated.By similarity
Proteolytically cleaved into 2 subunits, an extracellular alpha subunit and a seven-transmembrane subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei455 – 4562CleavageBy similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ923X1.
PaxDbiQ923X1.
PRIDEiQ923X1.

PTM databases

PhosphoSiteiQ923X1.

Expressioni

Inductioni

Induced by VEGF and FGF2.1 Publication

Gene expression databases

BgeeiQ923X1.
ExpressionAtlasiQ923X1. baseline and differential.
GenevisibleiQ923X1. MM.

Interactioni

Subunit structurei

Heterodimer of 2 chains generated by proteolytic processing; the large extracellular N-terminal fragment and the membrane-bound C-terminal fragment predominantly remain associated and non-covalently linked.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000041939.

Structurei

3D structure databases

ProteinModelPortaliQ923X1.
SMRiQ923X1. Positions 20-455, 502-723.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini20 – 5738EGF-like 1PROSITE-ProRule annotationAdd
BLAST
Domaini58 – 10750EGF-like 2; calcium-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini108 – 15750EGF-like 3; calcium-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini415 – 46753GPSPROSITE-ProRule annotationAdd
BLAST

Domaini

The transmembrane domain is not required for cleavage, but it is required for dimer formation.By similarity

Sequence similaritiesi

Contains 3 EGF-like domains.PROSITE-ProRule annotation
Contains 1 GPS domain.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4193. Eukaryota.
KOG4291. Eukaryota.
ENOG410XSD2. LUCA.
GeneTreeiENSGT00830000128227.
HOGENOMiHOG000112361.
HOVERGENiHBG101117.
InParanoidiQ923X1.
KOiK04595.
OMAiHLTKLMH.
OrthoDBiEOG76DTRK.
TreeFamiTF316380.

Family and domain databases

InterProiIPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR018097. EGF_Ca-bd_CS.
IPR032471. GAIN_dom_N.
IPR017981. GPCR_2-like.
IPR000832. GPCR_2_secretin-like.
IPR017983. GPCR_2_secretin-like_CS.
IPR000203. GPS.
[Graphical view]
PfamiPF00002. 7tm_2. 1 hit.
PF07645. EGF_CA. 2 hits.
PF16489. GAIN. 1 hit.
PF01825. GPS. 1 hit.
[Graphical view]
PRINTSiPR00249. GPCRSECRETIN.
SMARTiSM00181. EGF. 3 hits.
SM00179. EGF_CA. 2 hits.
SM00303. GPS. 1 hit.
[Graphical view]
PROSITEiPS00010. ASX_HYDROXYL. 2 hits.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 3 hits.
PS01187. EGF_CA. 2 hits.
PS00650. G_PROTEIN_RECEP_F2_2. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
PS50221. GPS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q923X1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLLPLLVGF STLLNCSYTQ NCSKTTCLPN AKCEVHNGVE ACFCSQGYSG
60 70 80 90 100
NGVTICEDID ECSESSVCGD HAVCENVNGG FSCFCREGYQ TATGKSQFTP
110 120 130 140 150
NDGSYCQDID ECSESSVCGD HAVCENVNGG FSCFCREGYQ TATGKSQFTP
160 170 180 190 200
NDGSYCQESM NSNCHLEHAC IAANINKTLK RIGPITEQTT LLQEIYRNSE
210 220 230 240 250
AELSLMDIVT YIEILTESSS LLGHPNSTTS YKDAHFNSTL TEFGETINNF
260 270 280 290 300
VERSTHKMWD QLPTNHRRLH LTKLMHTAEL VTLQIAQNTQ KNSQFDMNST
310 320 330 340 350
DLALKVFAFD STHMKHAHPH MNVDGGYVKI SPRRKAAHGT TGNVVVAFLC
360 370 380 390 400
YKSIGPLLSS SDNFLLDTQN DNSEGKEKVI SSVISASISS NPPTLYELEK
410 420 430 440 450
ITFTLSHVKL SDKHRTQCAF WNYSVDAMNN GSWSTEGCEL THSNDTHTSC
460 470 480 490 500
RCSHLTHFAI LMSSTSSIGI KDYNILTRIT QLGIIISLIC LAICIFTFWF
510 520 530 540 550
FSEIQSTRTT IHKNLCCSLF LAELVFLIGI NINTNKLVCS IIAGLLHYFF
560 570 580 590 600
LAAFAWMCIE GIHLYLIVVG VIYNKGFLHK NFYIFGYLSP AVVVGFSASL
610 620 630 640 650
GYRYYGTTKV CWLSTENNFI WSFIGPACLI ILVNLLAFGV IIYKVFRHTA
660 670 680 690 700
GLKPEVSCYE NIRSCARGAL ALLFLLGTTW IFGVLHVVHA SVVTAYLFTV
710 720 730
SNAFQGMFIF LFLCVLSRKI QEEYYRLFKN VPCCFGCLR
Length:739
Mass (Da):82,246
Last modified:July 27, 2011 - v3
Checksum:i1AEB95CCCB73B095
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti289 – 2891T → I in AAK62363 (PubMed:11438738).Curated
Sequence conflicti313 – 3131Missing in AAH17134 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF385682 mRNA. Translation: AAK62363.1.
AC127233 Genomic DNA. No translation available.
BC017134 mRNA. Translation: AAH17134.1.
CCDSiCCDS17910.1.
RefSeqiNP_573485.2. NM_133222.3.
UniGeneiMm.317261.

Genome annotation databases

EnsembliENSMUST00000046977; ENSMUSP00000041939; ENSMUSG00000039167.
GeneIDi170757.
KEGGimmu:170757.
UCSCiuc008rsj.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF385682 mRNA. Translation: AAK62363.1.
AC127233 Genomic DNA. No translation available.
BC017134 mRNA. Translation: AAH17134.1.
CCDSiCCDS17910.1.
RefSeqiNP_573485.2. NM_133222.3.
UniGeneiMm.317261.

3D structure databases

ProteinModelPortaliQ923X1.
SMRiQ923X1. Positions 20-455, 502-723.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000041939.

Protein family/group databases

MEROPSiP02.013.
GPCRDBiSearch...

PTM databases

PhosphoSiteiQ923X1.

Proteomic databases

MaxQBiQ923X1.
PaxDbiQ923X1.
PRIDEiQ923X1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000046977; ENSMUSP00000041939; ENSMUSG00000039167.
GeneIDi170757.
KEGGimmu:170757.
UCSCiuc008rsj.2. mouse.

Organism-specific databases

CTDi64123.
MGIiMGI:2655562. Adgrl4.

Phylogenomic databases

eggNOGiKOG4193. Eukaryota.
KOG4291. Eukaryota.
ENOG410XSD2. LUCA.
GeneTreeiENSGT00830000128227.
HOGENOMiHOG000112361.
HOVERGENiHBG101117.
InParanoidiQ923X1.
KOiK04595.
OMAiHLTKLMH.
OrthoDBiEOG76DTRK.
TreeFamiTF316380.

Miscellaneous databases

NextBioi370350.
PROiQ923X1.
SOURCEiSearch...

Gene expression databases

BgeeiQ923X1.
ExpressionAtlasiQ923X1. baseline and differential.
GenevisibleiQ923X1. MM.

Family and domain databases

InterProiIPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR018097. EGF_Ca-bd_CS.
IPR032471. GAIN_dom_N.
IPR017981. GPCR_2-like.
IPR000832. GPCR_2_secretin-like.
IPR017983. GPCR_2_secretin-like_CS.
IPR000203. GPS.
[Graphical view]
PfamiPF00002. 7tm_2. 1 hit.
PF07645. EGF_CA. 2 hits.
PF16489. GAIN. 1 hit.
PF01825. GPS. 1 hit.
[Graphical view]
PRINTSiPR00249. GPCRSECRETIN.
SMARTiSM00181. EGF. 3 hits.
SM00179. EGF_CA. 2 hits.
SM00303. GPS. 1 hit.
[Graphical view]
PROSITEiPS00010. ASX_HYDROXYL. 2 hits.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 3 hits.
PS01187. EGF_CA. 2 hits.
PS00650. G_PROTEIN_RECEP_F2_2. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
PS50221. GPS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: AKR/J.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  4. "Augmented cardiac hypertrophy in response to pressure overload in mice lacking ELTD1."
    Xiao J., Jiang H., Zhang R., Fan G., Zhang Y., Jiang D., Li H.
    PLoS ONE 7:E35779-E35779(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.
  5. "A core human primary tumor angiogenesis signature identifies the endothelial orphan receptor ELTD1 as a key regulator of angiogenesis."
    Masiero M., Simoes F.C., Han H.D., Snell C., Peterkin T., Bridges E., Mangala L.S., Wu S.Y., Pradeep S., Li D., Han C., Dalton H., Lopez-Berestein G., Tuynman J.B., Mortensen N., Li J.L., Patient R., Sood A.K.
    , Banham A.H., Harris A.L., Buffa F.M.
    Cancer Cell 24:229-241(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION.

Entry informationi

Entry nameiAGRL4_MOUSE
AccessioniPrimary (citable) accession number: Q923X1
Secondary accession number(s): E9QLT4, Q91W44
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 16, 2004
Last sequence update: July 27, 2011
Last modified: May 11, 2016
This is version 125 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.