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Q923W9

- ADA33_MOUSE

UniProt

Q923W9 - ADA33_MOUSE

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Protein
Disintegrin and metalloproteinase domain-containing protein 33
Gene
Adam33
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Cofactori

Binds 1 zinc ion per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi134 – 1341Zinc; in inhibited form By similarity
Metal bindingi346 – 3461Zinc; catalytic By similarity
Active sitei347 – 3471 By similarity
Metal bindingi350 – 3501Zinc; catalytic By similarity
Metal bindingi356 – 3561Zinc; catalytic By similarity

GO - Molecular functioni

  1. metalloendopeptidase activity Source: UniProtKB
  2. zinc ion binding Source: UniProtKB

GO - Biological processi

  1. proteolysis Source: UniProtKB
  2. regulation of cell proliferation Source: UniProtKB
  3. regulation of cytokine biosynthetic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiM12.244.

Names & Taxonomyi

Protein namesi
Recommended name:
Disintegrin and metalloproteinase domain-containing protein 33 (EC:3.4.24.-)
Short name:
ADAM 33
Gene namesi
Name:Adam33
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 2

Organism-specific databases

MGIiMGI:1341813. Adam33.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini30 – 702673Extracellular Reviewed prediction
Add
BLAST
Transmembranei703 – 72321Helical; Reviewed prediction
Add
BLAST
Topological domaini724 – 79774Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2929 Reviewed prediction
Add
BLAST
Propeptidei30 – 204175 By similarity
PRO_0000029144Add
BLAST
Chaini205 – 797593Disintegrin and metalloproteinase domain-containing protein 33
PRO_0000029145Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi110 – 1101N-linked (GlcNAc...) Reviewed prediction
Glycosylationi232 – 2321N-linked (GlcNAc...) Reviewed prediction
Glycosylationi277 – 2771N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi321 ↔ 405 By similarity
Disulfide bondi361 ↔ 389 By similarity
Disulfide bondi362 ↔ 372 By similarity
Glycosylationi408 – 4081N-linked (GlcNAc...) Reviewed prediction
Glycosylationi449 – 4491N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi476 ↔ 496 By similarity
Glycosylationi645 – 6451N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi654 ↔ 664 By similarity
Disulfide bondi658 ↔ 670 By similarity
Disulfide bondi672 ↔ 681 By similarity

Post-translational modificationi

The precursor is cleaved by a furin endopeptidase By similarity.

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PRIDEiQ923W9.

PTM databases

PhosphoSiteiQ923W9.

Expressioni

Gene expression databases

ArrayExpressiQ923W9.
BgeeiQ923W9.
CleanExiMM_ADAM33.
GenevestigatoriQ923W9.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000105861.

Structurei

3D structure databases

ProteinModelPortaliQ923W9.
SMRiQ923W9. Positions 205-683.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini211 – 410200Peptidase M12B
Add
BLAST
Domaini418 – 50487Disintegrin
Add
BLAST
Domaini650 – 68233EGF-like
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi132 – 1398Cysteine switch By similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi504 – 649146Cys-rich
Add
BLAST
Compositional biasi767 – 7726Poly-Pro

Domaini

The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

Sequence similaritiesi

Contains 1 disintegrin domain.
Contains 1 EGF-like domain.

Keywords - Domaini

EGF-like domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG294463.
GeneTreeiENSGT00740000114848.
HOGENOMiHOG000230883.
HOVERGENiHBG006978.
InParanoidiQ8R5G5.
KOiK08616.
OMAiLPCAGRD.
OrthoDBiEOG7F7W89.
PhylomeDBiQ923W9.
TreeFamiTF314733.

Family and domain databases

Gene3Di3.40.390.10. 1 hit.
4.10.70.10. 1 hit.
InterProiIPR006586. ADAM_Cys-rich.
IPR001762. Blood-coag_inhib_Disintegrin.
IPR018358. Disintegrin_CS.
IPR000742. EG-like_dom.
IPR013032. EGF-like_CS.
IPR024079. MetalloPept_cat_dom.
IPR001590. Peptidase_M12B.
IPR002870. Peptidase_M12B_N.
[Graphical view]
PfamiPF08516. ADAM_CR. 1 hit.
PF00200. Disintegrin. 1 hit.
PF01562. Pep_M12B_propep. 1 hit.
PF01421. Reprolysin. 1 hit.
[Graphical view]
PRINTSiPR00289. DISINTEGRIN.
SMARTiSM00608. ACR. 1 hit.
SM00050. DISIN. 1 hit.
SM00181. EGF. 1 hit.
[Graphical view]
SUPFAMiSSF57552. SSF57552. 1 hit.
PROSITEiPS50215. ADAM_MEPRO. 1 hit.
PS00427. DISINTEGRIN_1. 1 hit.
PS50214. DISINTEGRIN_2. 1 hit.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 1 hit.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q923W9-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MGSRCGRPGG SPVLLLLPLL LPSCPLRSAR MFPGNAHGEL VTPHWILEGR    50
LWLKVTLEEP ILKPDSVLVA LEAEGQDLLL ELEKKHKLLA PGYTETHYRP 100
DGHPVVLSPN HTDHCQYHGR VRGFRESWVV LSTCSGMSGL IVLSSKVSYY 150
LQPRTPGDTK DFPTHEIFRM EQLFTWRGVQ RDKNSQYKAG MASLPHVPQS 200
RVRREARRSP RYLELYIVAD HTLFLLQHQN LNHTRQRLLE VANCVDQILR 250
TLDIQLVLTG LEVWTEQDLS RITQDANETL WAFLQWRRGV WARRPHDSTQ 300
LLTGRTFQGT TVGLAPVEGI CRAESSGGVS TDHSELPIGT AATMAHEIGH 350
SLGLHHDPEG CCVQADAEQG GCVMEAATGH PFPRVFSACS RRQLRTFFRK 400
GGGPCLSNTS APGLLVLPSR CGNGFLEAGE ECDCGSGQKC PDPCCFAHNC 450
SLRAGAQCAH GDCCARCLLK SAGTPCRPAA TDCDLPEFCT GTSPYCPADV 500
YLLDGSPCAE GRGYCLDGWC PTLEQQCQQL WGPGSKPAPE PCFQQMNSMG 550
NSQGNCGQDH KGSFLPCAQR DALCGKLLCQ GGEPNPLVPH IVTMDSTILL 600
EGREVVCRGA FVLPDSHLDQ LDLGLVEPGT GCGPRMVCQD RHCQNATSQE 650
LERCLTACHN GGVCNSNRNC HCAAGWAPPF CDKPGLGGSV DSGPAQSANR 700
DAFPLAMLLS FLLPLLPGAG LAWCYYQLPT FCHRRGLCCR RDPLWNRDIP 750
LGSVHPVEFG SIITGEPSPP PPWTSCQQRS HPPSLDLLSD PANSELT 797
Length:797
Mass (Da):86,971
Last modified:July 27, 2011 - v3
Checksum:iB332EA909514E626
GO
Isoform 2 (identifier: Q923W9-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     637-662: Missing.

Show »
Length:771
Mass (Da):84,115
Checksum:iDAFFBA1D4416B5D2
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei637 – 66226Missing in isoform 2.
VSP_005496Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti68 – 681L → P in BAB84336. 1 Publication
Sequence conflicti68 – 681L → P in BAB84337. 1 Publication
Sequence conflicti319 – 3268GICRAESS → DMPRGELSF in AAK67164. 1 Publication
Sequence conflicti725 – 7251Missing in AAK67164. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB059633 mRNA. Translation: BAB84337.1.
AB059632 mRNA. Translation: BAB84336.1.
AF472524 mRNA. Translation: AAL79834.1.
AL833771 Genomic DNA. Translation: CAM18045.1.
CH466519 Genomic DNA. Translation: EDL28296.1.
AF386072 mRNA. Translation: AAK67164.1.
CCDSiCCDS16753.1. [Q923W9-1]
CCDS50716.1. [Q923W9-2]
RefSeqiNP_291093.2. NM_033615.2. [Q923W9-1]
UniGeneiMm.108550.

Genome annotation databases

EnsembliENSMUST00000052104; ENSMUSP00000052486; ENSMUSG00000027318. [Q923W9-2]
ENSMUST00000110232; ENSMUSP00000105861; ENSMUSG00000027318. [Q923W9-1]
ENSMUST00000183552; ENSMUSP00000139344; ENSMUSG00000027318. [Q923W9-1]
GeneIDi110751.
KEGGimmu:110751.
UCSCiuc008mkm.2. mouse. [Q923W9-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB059633 mRNA. Translation: BAB84337.1 .
AB059632 mRNA. Translation: BAB84336.1 .
AF472524 mRNA. Translation: AAL79834.1 .
AL833771 Genomic DNA. Translation: CAM18045.1 .
CH466519 Genomic DNA. Translation: EDL28296.1 .
AF386072 mRNA. Translation: AAK67164.1 .
CCDSi CCDS16753.1. [Q923W9-1 ]
CCDS50716.1. [Q923W9-2 ]
RefSeqi NP_291093.2. NM_033615.2. [Q923W9-1 ]
UniGenei Mm.108550.

3D structure databases

ProteinModelPortali Q923W9.
SMRi Q923W9. Positions 205-683.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10090.ENSMUSP00000105861.

Protein family/group databases

MEROPSi M12.244.

PTM databases

PhosphoSitei Q923W9.

Proteomic databases

PRIDEi Q923W9.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000052104 ; ENSMUSP00000052486 ; ENSMUSG00000027318 . [Q923W9-2 ]
ENSMUST00000110232 ; ENSMUSP00000105861 ; ENSMUSG00000027318 . [Q923W9-1 ]
ENSMUST00000183552 ; ENSMUSP00000139344 ; ENSMUSG00000027318 . [Q923W9-1 ]
GeneIDi 110751.
KEGGi mmu:110751.
UCSCi uc008mkm.2. mouse. [Q923W9-1 ]

Organism-specific databases

CTDi 80332.
MGIi MGI:1341813. Adam33.

Phylogenomic databases

eggNOGi NOG294463.
GeneTreei ENSGT00740000114848.
HOGENOMi HOG000230883.
HOVERGENi HBG006978.
InParanoidi Q8R5G5.
KOi K08616.
OMAi LPCAGRD.
OrthoDBi EOG7F7W89.
PhylomeDBi Q923W9.
TreeFami TF314733.

Miscellaneous databases

NextBioi 364593.
PROi Q923W9.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q923W9.
Bgeei Q923W9.
CleanExi MM_ADAM33.
Genevestigatori Q923W9.

Family and domain databases

Gene3Di 3.40.390.10. 1 hit.
4.10.70.10. 1 hit.
InterProi IPR006586. ADAM_Cys-rich.
IPR001762. Blood-coag_inhib_Disintegrin.
IPR018358. Disintegrin_CS.
IPR000742. EG-like_dom.
IPR013032. EGF-like_CS.
IPR024079. MetalloPept_cat_dom.
IPR001590. Peptidase_M12B.
IPR002870. Peptidase_M12B_N.
[Graphical view ]
Pfami PF08516. ADAM_CR. 1 hit.
PF00200. Disintegrin. 1 hit.
PF01562. Pep_M12B_propep. 1 hit.
PF01421. Reprolysin. 1 hit.
[Graphical view ]
PRINTSi PR00289. DISINTEGRIN.
SMARTi SM00608. ACR. 1 hit.
SM00050. DISIN. 1 hit.
SM00181. EGF. 1 hit.
[Graphical view ]
SUPFAMi SSF57552. SSF57552. 1 hit.
PROSITEi PS50215. ADAM_MEPRO. 1 hit.
PS00427. DISINTEGRIN_1. 1 hit.
PS50214. DISINTEGRIN_2. 1 hit.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 1 hit.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and characterization of novel mouse and human ADAM33s with potential metalloprotease activity."
    Yoshinaka T., Nishii K., Yamada K., Sawada H., Nishiwaki E., Smith K., Yoshino K., Ishiguro H., Higashiyama S.
    Gene 282:227-236(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
    Tissue: Brain.
  2. Karkkainen I., Liehu M.A., Huovila A.-P.J.
    Submitted (JAN-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Strain: NMRI.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "M-ADAM33 cloned from mouse embryo day 11 cDNA library."
    Smith K.M., Alfandari D., White J.M., Sutherland A.E., DeSimone D.W.
    Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 113-797 (ISOFORM 1).
    Strain: Swiss Webster / NIH.
  6. Lubec G., Kang S.U.
    Submitted (APR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 562-570 AND 780-797, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: C57BL/6.
    Tissue: Brain.

Entry informationi

Entry nameiADA33_MOUSE
AccessioniPrimary (citable) accession number: Q923W9
Secondary accession number(s): Q8R5G5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 116 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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