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Q923W9 (ADA33_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 116. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Disintegrin and metalloproteinase domain-containing protein 33

Short name=ADAM 33
EC=3.4.24.-
Gene names
Name:Adam33
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length797 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Cofactor

Binds 1 zinc ion per subunit By similarity.

Subcellular location

Membrane; Single-pass type I membrane protein.

Domain

The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

Post-translational modification

The precursor is cleaved by a furin endopeptidase By similarity.

Sequence similarities

Contains 1 disintegrin domain.

Contains 1 EGF-like domain.

Contains 1 peptidase M12B domain.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q923W9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q923W9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     637-662: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2929 Potential
Propeptide30 – 204175 By similarity
PRO_0000029144
Chain205 – 797593Disintegrin and metalloproteinase domain-containing protein 33
PRO_0000029145

Regions

Topological domain30 – 702673Extracellular Potential
Transmembrane703 – 72321Helical; Potential
Topological domain724 – 79774Cytoplasmic Potential
Domain211 – 410200Peptidase M12B
Domain418 – 50487Disintegrin
Domain650 – 68233EGF-like
Motif132 – 1398Cysteine switch By similarity
Compositional bias504 – 649146Cys-rich
Compositional bias767 – 7726Poly-Pro

Sites

Active site3471 By similarity
Metal binding1341Zinc; in inhibited form By similarity
Metal binding3461Zinc; catalytic By similarity
Metal binding3501Zinc; catalytic By similarity
Metal binding3561Zinc; catalytic By similarity

Amino acid modifications

Glycosylation1101N-linked (GlcNAc...) Potential
Glycosylation2321N-linked (GlcNAc...) Potential
Glycosylation2771N-linked (GlcNAc...) Potential
Glycosylation4081N-linked (GlcNAc...) Potential
Glycosylation4491N-linked (GlcNAc...) Potential
Glycosylation6451N-linked (GlcNAc...) Potential
Disulfide bond321 ↔ 405 By similarity
Disulfide bond361 ↔ 389 By similarity
Disulfide bond362 ↔ 372 By similarity
Disulfide bond476 ↔ 496 By similarity
Disulfide bond654 ↔ 664 By similarity
Disulfide bond658 ↔ 670 By similarity
Disulfide bond672 ↔ 681 By similarity

Natural variations

Alternative sequence637 – 66226Missing in isoform 2.
VSP_005496

Experimental info

Sequence conflict681L → P in BAB84336. Ref.1
Sequence conflict681L → P in BAB84337. Ref.1
Sequence conflict319 – 3268GICRAESS → DMPRGELSF in AAK67164. Ref.5
Sequence conflict7251Missing in AAK67164. Ref.5

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified July 27, 2011. Version 3.
Checksum: B332EA909514E626

FASTA79786,971
        10         20         30         40         50         60 
MGSRCGRPGG SPVLLLLPLL LPSCPLRSAR MFPGNAHGEL VTPHWILEGR LWLKVTLEEP 

        70         80         90        100        110        120 
ILKPDSVLVA LEAEGQDLLL ELEKKHKLLA PGYTETHYRP DGHPVVLSPN HTDHCQYHGR 

       130        140        150        160        170        180 
VRGFRESWVV LSTCSGMSGL IVLSSKVSYY LQPRTPGDTK DFPTHEIFRM EQLFTWRGVQ 

       190        200        210        220        230        240 
RDKNSQYKAG MASLPHVPQS RVRREARRSP RYLELYIVAD HTLFLLQHQN LNHTRQRLLE 

       250        260        270        280        290        300 
VANCVDQILR TLDIQLVLTG LEVWTEQDLS RITQDANETL WAFLQWRRGV WARRPHDSTQ 

       310        320        330        340        350        360 
LLTGRTFQGT TVGLAPVEGI CRAESSGGVS TDHSELPIGT AATMAHEIGH SLGLHHDPEG 

       370        380        390        400        410        420 
CCVQADAEQG GCVMEAATGH PFPRVFSACS RRQLRTFFRK GGGPCLSNTS APGLLVLPSR 

       430        440        450        460        470        480 
CGNGFLEAGE ECDCGSGQKC PDPCCFAHNC SLRAGAQCAH GDCCARCLLK SAGTPCRPAA 

       490        500        510        520        530        540 
TDCDLPEFCT GTSPYCPADV YLLDGSPCAE GRGYCLDGWC PTLEQQCQQL WGPGSKPAPE 

       550        560        570        580        590        600 
PCFQQMNSMG NSQGNCGQDH KGSFLPCAQR DALCGKLLCQ GGEPNPLVPH IVTMDSTILL 

       610        620        630        640        650        660 
EGREVVCRGA FVLPDSHLDQ LDLGLVEPGT GCGPRMVCQD RHCQNATSQE LERCLTACHN 

       670        680        690        700        710        720 
GGVCNSNRNC HCAAGWAPPF CDKPGLGGSV DSGPAQSANR DAFPLAMLLS FLLPLLPGAG 

       730        740        750        760        770        780 
LAWCYYQLPT FCHRRGLCCR RDPLWNRDIP LGSVHPVEFG SIITGEPSPP PPWTSCQQRS 

       790 
HPPSLDLLSD PANSELT 

« Hide

Isoform 2 [UniParc].

Checksum: DAFFBA1D4416B5D2
Show »

FASTA77184,115

References

« Hide 'large scale' references
[1]"Identification and characterization of novel mouse and human ADAM33s with potential metalloprotease activity."
Yoshinaka T., Nishii K., Yamada K., Sawada H., Nishiwaki E., Smith K., Yoshino K., Ishiguro H., Higashiyama S.
Gene 282:227-236(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
Tissue: Brain.
[2]Karkkainen I., Liehu M.A., Huovila A.-P.J.
Submitted (JAN-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Strain: NMRI.
[3]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[4]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"M-ADAM33 cloned from mouse embryo day 11 cDNA library."
Smith K.M., Alfandari D., White J.M., Sutherland A.E., DeSimone D.W.
Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 113-797 (ISOFORM 1).
Strain: Swiss Webster / NIH.
[6]Lubec G., Kang S.U.
Submitted (APR-2007) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 562-570 AND 780-797, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: C57BL/6.
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB059633 mRNA. Translation: BAB84337.1.
AB059632 mRNA. Translation: BAB84336.1.
AF472524 mRNA. Translation: AAL79834.1.
AL833771 Genomic DNA. Translation: CAM18045.1.
CH466519 Genomic DNA. Translation: EDL28296.1.
AF386072 mRNA. Translation: AAK67164.1.
CCDSCCDS16753.1. [Q923W9-1]
CCDS50716.1. [Q923W9-2]
RefSeqNP_291093.2. NM_033615.2. [Q923W9-1]
UniGeneMm.108550.

3D structure databases

ProteinModelPortalQ923W9.
SMRQ923W9. Positions 205-683.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000105861.

Protein family/group databases

MEROPSM12.244.

PTM databases

PhosphoSiteQ923W9.

Proteomic databases

PRIDEQ923W9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000052104; ENSMUSP00000052486; ENSMUSG00000027318. [Q923W9-2]
ENSMUST00000110232; ENSMUSP00000105861; ENSMUSG00000027318. [Q923W9-1]
ENSMUST00000183552; ENSMUSP00000139344; ENSMUSG00000027318. [Q923W9-1]
GeneID110751.
KEGGmmu:110751.
UCSCuc008mkm.2. mouse. [Q923W9-1]

Organism-specific databases

CTD80332.
MGIMGI:1341813. Adam33.

Phylogenomic databases

eggNOGNOG294463.
GeneTreeENSGT00740000114848.
HOGENOMHOG000230883.
HOVERGENHBG006978.
InParanoidQ8R5G5.
KOK08616.
OMALPCAGRD.
OrthoDBEOG7F7W89.
PhylomeDBQ923W9.
TreeFamTF314733.

Gene expression databases

ArrayExpressQ923W9.
BgeeQ923W9.
CleanExMM_ADAM33.
GenevestigatorQ923W9.

Family and domain databases

Gene3D3.40.390.10. 1 hit.
4.10.70.10. 1 hit.
InterProIPR006586. ADAM_Cys-rich.
IPR001762. Blood-coag_inhib_Disintegrin.
IPR018358. Disintegrin_CS.
IPR000742. EG-like_dom.
IPR013032. EGF-like_CS.
IPR024079. MetalloPept_cat_dom.
IPR001590. Peptidase_M12B.
IPR002870. Peptidase_M12B_N.
[Graphical view]
PfamPF08516. ADAM_CR. 1 hit.
PF00200. Disintegrin. 1 hit.
PF01562. Pep_M12B_propep. 1 hit.
PF01421. Reprolysin. 1 hit.
[Graphical view]
PRINTSPR00289. DISINTEGRIN.
SMARTSM00608. ACR. 1 hit.
SM00050. DISIN. 1 hit.
SM00181. EGF. 1 hit.
[Graphical view]
SUPFAMSSF57552. SSF57552. 1 hit.
PROSITEPS50215. ADAM_MEPRO. 1 hit.
PS00427. DISINTEGRIN_1. 1 hit.
PS50214. DISINTEGRIN_2. 1 hit.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 1 hit.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio364593.
PROQ923W9.
SOURCESearch...

Entry information

Entry nameADA33_MOUSE
AccessionPrimary (citable) accession number: Q923W9
Secondary accession number(s): Q8R5G5
Entry history
Integrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 116 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot