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Q923W9

- ADA33_MOUSE

UniProt

Q923W9 - ADA33_MOUSE

Protein

Disintegrin and metalloproteinase domain-containing protein 33

Gene

Adam33

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 117 (01 Oct 2014)
      Sequence version 3 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Cofactori

    Binds 1 zinc ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi134 – 1341Zinc; in inhibited formBy similarity
    Metal bindingi346 – 3461Zinc; catalyticBy similarity
    Active sitei347 – 3471PROSITE-ProRule annotation
    Metal bindingi350 – 3501Zinc; catalyticBy similarity
    Metal bindingi356 – 3561Zinc; catalyticBy similarity

    GO - Molecular functioni

    1. metalloendopeptidase activity Source: UniProtKB
    2. zinc ion binding Source: UniProtKB

    GO - Biological processi

    1. proteolysis Source: UniProtKB
    2. regulation of cell proliferation Source: UniProtKB
    3. regulation of cytokine biosynthetic process Source: UniProtKB

    Keywords - Molecular functioni

    Hydrolase, Metalloprotease, Protease

    Keywords - Ligandi

    Metal-binding, Zinc

    Protein family/group databases

    MEROPSiM12.244.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Disintegrin and metalloproteinase domain-containing protein 33 (EC:3.4.24.-)
    Short name:
    ADAM 33
    Gene namesi
    Name:Adam33
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 2

    Organism-specific databases

    MGIiMGI:1341813. Adam33.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2929Sequence AnalysisAdd
    BLAST
    Propeptidei30 – 204175By similarityPRO_0000029144Add
    BLAST
    Chaini205 – 797593Disintegrin and metalloproteinase domain-containing protein 33PRO_0000029145Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi110 – 1101N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi232 – 2321N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi277 – 2771N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi321 ↔ 405By similarity
    Disulfide bondi361 ↔ 389By similarity
    Disulfide bondi362 ↔ 372By similarity
    Glycosylationi408 – 4081N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi449 – 4491N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi476 ↔ 496By similarity
    Glycosylationi645 – 6451N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi654 ↔ 664By similarity
    Disulfide bondi658 ↔ 670By similarity
    Disulfide bondi672 ↔ 681By similarity

    Post-translational modificationi

    The precursor is cleaved by a furin endopeptidase.By similarity

    Keywords - PTMi

    Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Zymogen

    Proteomic databases

    PRIDEiQ923W9.

    PTM databases

    PhosphoSiteiQ923W9.

    Expressioni

    Gene expression databases

    ArrayExpressiQ923W9.
    BgeeiQ923W9.
    CleanExiMM_ADAM33.
    GenevestigatoriQ923W9.

    Interactioni

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000105861.

    Structurei

    3D structure databases

    ProteinModelPortaliQ923W9.
    SMRiQ923W9. Positions 205-683.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini30 – 702673ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini724 – 79774CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei703 – 72321HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini211 – 410200Peptidase M12BPROSITE-ProRule annotationAdd
    BLAST
    Domaini418 – 50487DisintegrinPROSITE-ProRule annotationAdd
    BLAST
    Domaini650 – 68233EGF-likePROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi132 – 1398Cysteine switchBy similarity

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi504 – 649146Cys-richAdd
    BLAST
    Compositional biasi767 – 7726Poly-Pro

    Domaini

    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

    Sequence similaritiesi

    Contains 1 disintegrin domain.PROSITE-ProRule annotation
    Contains 1 EGF-like domain.PROSITE-ProRule annotation
    Contains 1 peptidase M12B domain.PROSITE-ProRule annotation

    Keywords - Domaini

    EGF-like domain, Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG294463.
    GeneTreeiENSGT00740000114848.
    HOGENOMiHOG000230883.
    HOVERGENiHBG006978.
    InParanoidiQ8R5G5.
    KOiK08616.
    OMAiLPCAGRD.
    OrthoDBiEOG7F7W89.
    PhylomeDBiQ923W9.
    TreeFamiTF314733.

    Family and domain databases

    Gene3Di3.40.390.10. 1 hit.
    4.10.70.10. 1 hit.
    InterProiIPR006586. ADAM_Cys-rich.
    IPR001762. Blood-coag_inhib_Disintegrin.
    IPR018358. Disintegrin_CS.
    IPR000742. EG-like_dom.
    IPR013032. EGF-like_CS.
    IPR024079. MetalloPept_cat_dom.
    IPR001590. Peptidase_M12B.
    IPR002870. Peptidase_M12B_N.
    [Graphical view]
    PfamiPF08516. ADAM_CR. 1 hit.
    PF00200. Disintegrin. 1 hit.
    PF01562. Pep_M12B_propep. 1 hit.
    PF01421. Reprolysin. 1 hit.
    [Graphical view]
    PRINTSiPR00289. DISINTEGRIN.
    SMARTiSM00608. ACR. 1 hit.
    SM00050. DISIN. 1 hit.
    SM00181. EGF. 1 hit.
    [Graphical view]
    SUPFAMiSSF57552. SSF57552. 1 hit.
    PROSITEiPS50215. ADAM_MEPRO. 1 hit.
    PS00427. DISINTEGRIN_1. 1 hit.
    PS50214. DISINTEGRIN_2. 1 hit.
    PS01186. EGF_2. 1 hit.
    PS50026. EGF_3. 1 hit.
    PS00142. ZINC_PROTEASE. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q923W9-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MGSRCGRPGG SPVLLLLPLL LPSCPLRSAR MFPGNAHGEL VTPHWILEGR    50
    LWLKVTLEEP ILKPDSVLVA LEAEGQDLLL ELEKKHKLLA PGYTETHYRP 100
    DGHPVVLSPN HTDHCQYHGR VRGFRESWVV LSTCSGMSGL IVLSSKVSYY 150
    LQPRTPGDTK DFPTHEIFRM EQLFTWRGVQ RDKNSQYKAG MASLPHVPQS 200
    RVRREARRSP RYLELYIVAD HTLFLLQHQN LNHTRQRLLE VANCVDQILR 250
    TLDIQLVLTG LEVWTEQDLS RITQDANETL WAFLQWRRGV WARRPHDSTQ 300
    LLTGRTFQGT TVGLAPVEGI CRAESSGGVS TDHSELPIGT AATMAHEIGH 350
    SLGLHHDPEG CCVQADAEQG GCVMEAATGH PFPRVFSACS RRQLRTFFRK 400
    GGGPCLSNTS APGLLVLPSR CGNGFLEAGE ECDCGSGQKC PDPCCFAHNC 450
    SLRAGAQCAH GDCCARCLLK SAGTPCRPAA TDCDLPEFCT GTSPYCPADV 500
    YLLDGSPCAE GRGYCLDGWC PTLEQQCQQL WGPGSKPAPE PCFQQMNSMG 550
    NSQGNCGQDH KGSFLPCAQR DALCGKLLCQ GGEPNPLVPH IVTMDSTILL 600
    EGREVVCRGA FVLPDSHLDQ LDLGLVEPGT GCGPRMVCQD RHCQNATSQE 650
    LERCLTACHN GGVCNSNRNC HCAAGWAPPF CDKPGLGGSV DSGPAQSANR 700
    DAFPLAMLLS FLLPLLPGAG LAWCYYQLPT FCHRRGLCCR RDPLWNRDIP 750
    LGSVHPVEFG SIITGEPSPP PPWTSCQQRS HPPSLDLLSD PANSELT 797
    Length:797
    Mass (Da):86,971
    Last modified:July 27, 2011 - v3
    Checksum:iB332EA909514E626
    GO
    Isoform 2 (identifier: Q923W9-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         637-662: Missing.

    Show »
    Length:771
    Mass (Da):84,115
    Checksum:iDAFFBA1D4416B5D2
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti68 – 681L → P in BAB84336. (PubMed:11814695)Curated
    Sequence conflicti68 – 681L → P in BAB84337. (PubMed:11814695)Curated
    Sequence conflicti319 – 3268GICRAESS → DMPRGELSF in AAK67164. 1 PublicationCurated
    Sequence conflicti725 – 7251Missing in AAK67164. 1 PublicationCurated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei637 – 66226Missing in isoform 2. 1 PublicationVSP_005496Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB059633 mRNA. Translation: BAB84337.1.
    AB059632 mRNA. Translation: BAB84336.1.
    AF472524 mRNA. Translation: AAL79834.1.
    AL833771 Genomic DNA. Translation: CAM18045.1.
    CH466519 Genomic DNA. Translation: EDL28296.1.
    AF386072 mRNA. Translation: AAK67164.1.
    CCDSiCCDS16753.1. [Q923W9-1]
    CCDS50716.1. [Q923W9-2]
    RefSeqiNP_291093.2. NM_033615.2. [Q923W9-1]
    UniGeneiMm.108550.

    Genome annotation databases

    EnsembliENSMUST00000052104; ENSMUSP00000052486; ENSMUSG00000027318. [Q923W9-2]
    ENSMUST00000110232; ENSMUSP00000105861; ENSMUSG00000027318. [Q923W9-1]
    ENSMUST00000183552; ENSMUSP00000139344; ENSMUSG00000027318. [Q923W9-1]
    GeneIDi110751.
    KEGGimmu:110751.
    UCSCiuc008mkm.2. mouse. [Q923W9-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB059633 mRNA. Translation: BAB84337.1 .
    AB059632 mRNA. Translation: BAB84336.1 .
    AF472524 mRNA. Translation: AAL79834.1 .
    AL833771 Genomic DNA. Translation: CAM18045.1 .
    CH466519 Genomic DNA. Translation: EDL28296.1 .
    AF386072 mRNA. Translation: AAK67164.1 .
    CCDSi CCDS16753.1. [Q923W9-1 ]
    CCDS50716.1. [Q923W9-2 ]
    RefSeqi NP_291093.2. NM_033615.2. [Q923W9-1 ]
    UniGenei Mm.108550.

    3D structure databases

    ProteinModelPortali Q923W9.
    SMRi Q923W9. Positions 205-683.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000105861.

    Protein family/group databases

    MEROPSi M12.244.

    PTM databases

    PhosphoSitei Q923W9.

    Proteomic databases

    PRIDEi Q923W9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000052104 ; ENSMUSP00000052486 ; ENSMUSG00000027318 . [Q923W9-2 ]
    ENSMUST00000110232 ; ENSMUSP00000105861 ; ENSMUSG00000027318 . [Q923W9-1 ]
    ENSMUST00000183552 ; ENSMUSP00000139344 ; ENSMUSG00000027318 . [Q923W9-1 ]
    GeneIDi 110751.
    KEGGi mmu:110751.
    UCSCi uc008mkm.2. mouse. [Q923W9-1 ]

    Organism-specific databases

    CTDi 80332.
    MGIi MGI:1341813. Adam33.

    Phylogenomic databases

    eggNOGi NOG294463.
    GeneTreei ENSGT00740000114848.
    HOGENOMi HOG000230883.
    HOVERGENi HBG006978.
    InParanoidi Q8R5G5.
    KOi K08616.
    OMAi LPCAGRD.
    OrthoDBi EOG7F7W89.
    PhylomeDBi Q923W9.
    TreeFami TF314733.

    Miscellaneous databases

    NextBioi 364593.
    PROi Q923W9.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q923W9.
    Bgeei Q923W9.
    CleanExi MM_ADAM33.
    Genevestigatori Q923W9.

    Family and domain databases

    Gene3Di 3.40.390.10. 1 hit.
    4.10.70.10. 1 hit.
    InterProi IPR006586. ADAM_Cys-rich.
    IPR001762. Blood-coag_inhib_Disintegrin.
    IPR018358. Disintegrin_CS.
    IPR000742. EG-like_dom.
    IPR013032. EGF-like_CS.
    IPR024079. MetalloPept_cat_dom.
    IPR001590. Peptidase_M12B.
    IPR002870. Peptidase_M12B_N.
    [Graphical view ]
    Pfami PF08516. ADAM_CR. 1 hit.
    PF00200. Disintegrin. 1 hit.
    PF01562. Pep_M12B_propep. 1 hit.
    PF01421. Reprolysin. 1 hit.
    [Graphical view ]
    PRINTSi PR00289. DISINTEGRIN.
    SMARTi SM00608. ACR. 1 hit.
    SM00050. DISIN. 1 hit.
    SM00181. EGF. 1 hit.
    [Graphical view ]
    SUPFAMi SSF57552. SSF57552. 1 hit.
    PROSITEi PS50215. ADAM_MEPRO. 1 hit.
    PS00427. DISINTEGRIN_1. 1 hit.
    PS50214. DISINTEGRIN_2. 1 hit.
    PS01186. EGF_2. 1 hit.
    PS50026. EGF_3. 1 hit.
    PS00142. ZINC_PROTEASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification and characterization of novel mouse and human ADAM33s with potential metalloprotease activity."
      Yoshinaka T., Nishii K., Yamada K., Sawada H., Nishiwaki E., Smith K., Yoshino K., Ishiguro H., Higashiyama S.
      Gene 282:227-236(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
      Tissue: Brain.
    2. Karkkainen I., Liehu M.A., Huovila A.-P.J.
      Submitted (JAN-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Strain: NMRI.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "M-ADAM33 cloned from mouse embryo day 11 cDNA library."
      Smith K.M., Alfandari D., White J.M., Sutherland A.E., DeSimone D.W.
      Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 113-797 (ISOFORM 1).
      Strain: Swiss Webster / NIH.
    6. Lubec G., Kang S.U.
      Submitted (APR-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 562-570 AND 780-797, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: C57BL/6.
      Tissue: Brain.

    Entry informationi

    Entry nameiADA33_MOUSE
    AccessioniPrimary (citable) accession number: Q923W9
    Secondary accession number(s): Q8R5G5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 27, 2002
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 117 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3