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Q923W9

- ADA33_MOUSE

UniProt

Q923W9 - ADA33_MOUSE

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Protein

Disintegrin and metalloproteinase domain-containing protein 33

Gene

Adam33

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Cofactori

Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi134 – 1341Zinc; in inhibited formBy similarity
Metal bindingi346 – 3461Zinc; catalyticBy similarity
Active sitei347 – 3471PROSITE-ProRule annotation
Metal bindingi350 – 3501Zinc; catalyticBy similarity
Metal bindingi356 – 3561Zinc; catalyticBy similarity

GO - Molecular functioni

  1. metalloendopeptidase activity Source: UniProtKB
  2. zinc ion binding Source: UniProtKB

GO - Biological processi

  1. proteolysis Source: UniProtKB
  2. regulation of cell proliferation Source: UniProtKB
  3. regulation of cytokine biosynthetic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiM12.244.

Names & Taxonomyi

Protein namesi
Recommended name:
Disintegrin and metalloproteinase domain-containing protein 33 (EC:3.4.24.-)
Short name:
ADAM 33
Gene namesi
Name:Adam33
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 2

Organism-specific databases

MGIiMGI:1341813. Adam33.

Subcellular locationi

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2929Sequence AnalysisAdd
BLAST
Propeptidei30 – 204175By similarityPRO_0000029144Add
BLAST
Chaini205 – 797593Disintegrin and metalloproteinase domain-containing protein 33PRO_0000029145Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi110 – 1101N-linked (GlcNAc...)Sequence Analysis
Glycosylationi232 – 2321N-linked (GlcNAc...)Sequence Analysis
Glycosylationi277 – 2771N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi321 ↔ 405By similarity
Disulfide bondi361 ↔ 389By similarity
Disulfide bondi362 ↔ 372By similarity
Glycosylationi408 – 4081N-linked (GlcNAc...)Sequence Analysis
Glycosylationi449 – 4491N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi476 ↔ 496By similarity
Glycosylationi645 – 6451N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi654 ↔ 664By similarity
Disulfide bondi658 ↔ 670By similarity
Disulfide bondi672 ↔ 681By similarity

Post-translational modificationi

The precursor is cleaved by a furin endopeptidase.By similarity

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PRIDEiQ923W9.

PTM databases

PhosphoSiteiQ923W9.

Expressioni

Gene expression databases

BgeeiQ923W9.
CleanExiMM_ADAM33.
ExpressionAtlasiQ923W9. baseline and differential.
GenevestigatoriQ923W9.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000105861.

Structurei

3D structure databases

ProteinModelPortaliQ923W9.
SMRiQ923W9. Positions 205-682.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini30 – 702673ExtracellularSequence AnalysisAdd
BLAST
Topological domaini724 – 79774CytoplasmicSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei703 – 72321HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini211 – 410200Peptidase M12BPROSITE-ProRule annotationAdd
BLAST
Domaini418 – 50487DisintegrinPROSITE-ProRule annotationAdd
BLAST
Domaini650 – 68233EGF-likePROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi132 – 1398Cysteine switchBy similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi504 – 649146Cys-richAdd
BLAST
Compositional biasi767 – 7726Poly-Pro

Domaini

The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

Sequence similaritiesi

Contains 1 disintegrin domain.PROSITE-ProRule annotation
Contains 1 EGF-like domain.PROSITE-ProRule annotation
Contains 1 peptidase M12B domain.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG294463.
GeneTreeiENSGT00760000118888.
HOGENOMiHOG000230883.
HOVERGENiHBG006978.
InParanoidiQ923W9.
KOiK08616.
OMAiLPCAGRD.
OrthoDBiEOG7F7W89.
PhylomeDBiQ923W9.
TreeFamiTF314733.

Family and domain databases

Gene3Di3.40.390.10. 1 hit.
4.10.70.10. 1 hit.
InterProiIPR006586. ADAM_Cys-rich.
IPR001762. Blood-coag_inhib_Disintegrin.
IPR018358. Disintegrin_CS.
IPR000742. EG-like_dom.
IPR013032. EGF-like_CS.
IPR024079. MetalloPept_cat_dom.
IPR001590. Peptidase_M12B.
IPR002870. Peptidase_M12B_N.
[Graphical view]
PfamiPF08516. ADAM_CR. 1 hit.
PF00200. Disintegrin. 1 hit.
PF01562. Pep_M12B_propep. 1 hit.
PF01421. Reprolysin. 1 hit.
[Graphical view]
PRINTSiPR00289. DISINTEGRIN.
SMARTiSM00608. ACR. 1 hit.
SM00050. DISIN. 1 hit.
SM00181. EGF. 1 hit.
[Graphical view]
SUPFAMiSSF57552. SSF57552. 1 hit.
PROSITEiPS50215. ADAM_MEPRO. 1 hit.
PS00427. DISINTEGRIN_1. 1 hit.
PS50214. DISINTEGRIN_2. 1 hit.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 1 hit.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q923W9-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGSRCGRPGG SPVLLLLPLL LPSCPLRSAR MFPGNAHGEL VTPHWILEGR
60 70 80 90 100
LWLKVTLEEP ILKPDSVLVA LEAEGQDLLL ELEKKHKLLA PGYTETHYRP
110 120 130 140 150
DGHPVVLSPN HTDHCQYHGR VRGFRESWVV LSTCSGMSGL IVLSSKVSYY
160 170 180 190 200
LQPRTPGDTK DFPTHEIFRM EQLFTWRGVQ RDKNSQYKAG MASLPHVPQS
210 220 230 240 250
RVRREARRSP RYLELYIVAD HTLFLLQHQN LNHTRQRLLE VANCVDQILR
260 270 280 290 300
TLDIQLVLTG LEVWTEQDLS RITQDANETL WAFLQWRRGV WARRPHDSTQ
310 320 330 340 350
LLTGRTFQGT TVGLAPVEGI CRAESSGGVS TDHSELPIGT AATMAHEIGH
360 370 380 390 400
SLGLHHDPEG CCVQADAEQG GCVMEAATGH PFPRVFSACS RRQLRTFFRK
410 420 430 440 450
GGGPCLSNTS APGLLVLPSR CGNGFLEAGE ECDCGSGQKC PDPCCFAHNC
460 470 480 490 500
SLRAGAQCAH GDCCARCLLK SAGTPCRPAA TDCDLPEFCT GTSPYCPADV
510 520 530 540 550
YLLDGSPCAE GRGYCLDGWC PTLEQQCQQL WGPGSKPAPE PCFQQMNSMG
560 570 580 590 600
NSQGNCGQDH KGSFLPCAQR DALCGKLLCQ GGEPNPLVPH IVTMDSTILL
610 620 630 640 650
EGREVVCRGA FVLPDSHLDQ LDLGLVEPGT GCGPRMVCQD RHCQNATSQE
660 670 680 690 700
LERCLTACHN GGVCNSNRNC HCAAGWAPPF CDKPGLGGSV DSGPAQSANR
710 720 730 740 750
DAFPLAMLLS FLLPLLPGAG LAWCYYQLPT FCHRRGLCCR RDPLWNRDIP
760 770 780 790
LGSVHPVEFG SIITGEPSPP PPWTSCQQRS HPPSLDLLSD PANSELT
Length:797
Mass (Da):86,971
Last modified:July 27, 2011 - v3
Checksum:iB332EA909514E626
GO
Isoform 2 (identifier: Q923W9-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     637-662: Missing.

Show »
Length:771
Mass (Da):84,115
Checksum:iDAFFBA1D4416B5D2
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti68 – 681L → P in BAB84336. (PubMed:11814695)Curated
Sequence conflicti68 – 681L → P in BAB84337. (PubMed:11814695)Curated
Sequence conflicti319 – 3268GICRAESS → DMPRGELSF in AAK67164. 1 PublicationCurated
Sequence conflicti725 – 7251Missing in AAK67164. 1 PublicationCurated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei637 – 66226Missing in isoform 2. 1 PublicationVSP_005496Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB059633 mRNA. Translation: BAB84337.1.
AB059632 mRNA. Translation: BAB84336.1.
AF472524 mRNA. Translation: AAL79834.1.
AL833771 Genomic DNA. Translation: CAM18045.1.
CH466519 Genomic DNA. Translation: EDL28296.1.
AF386072 mRNA. Translation: AAK67164.1.
CCDSiCCDS16753.1. [Q923W9-1]
CCDS50716.1. [Q923W9-2]
RefSeqiNP_291093.2. NM_033615.2. [Q923W9-1]
UniGeneiMm.108550.

Genome annotation databases

EnsembliENSMUST00000052104; ENSMUSP00000052486; ENSMUSG00000027318. [Q923W9-2]
ENSMUST00000110232; ENSMUSP00000105861; ENSMUSG00000027318. [Q923W9-1]
ENSMUST00000183552; ENSMUSP00000139344; ENSMUSG00000027318. [Q923W9-1]
GeneIDi110751.
KEGGimmu:110751.
UCSCiuc008mkm.2. mouse. [Q923W9-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB059633 mRNA. Translation: BAB84337.1 .
AB059632 mRNA. Translation: BAB84336.1 .
AF472524 mRNA. Translation: AAL79834.1 .
AL833771 Genomic DNA. Translation: CAM18045.1 .
CH466519 Genomic DNA. Translation: EDL28296.1 .
AF386072 mRNA. Translation: AAK67164.1 .
CCDSi CCDS16753.1. [Q923W9-1 ]
CCDS50716.1. [Q923W9-2 ]
RefSeqi NP_291093.2. NM_033615.2. [Q923W9-1 ]
UniGenei Mm.108550.

3D structure databases

ProteinModelPortali Q923W9.
SMRi Q923W9. Positions 205-682.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10090.ENSMUSP00000105861.

Protein family/group databases

MEROPSi M12.244.

PTM databases

PhosphoSitei Q923W9.

Proteomic databases

PRIDEi Q923W9.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000052104 ; ENSMUSP00000052486 ; ENSMUSG00000027318 . [Q923W9-2 ]
ENSMUST00000110232 ; ENSMUSP00000105861 ; ENSMUSG00000027318 . [Q923W9-1 ]
ENSMUST00000183552 ; ENSMUSP00000139344 ; ENSMUSG00000027318 . [Q923W9-1 ]
GeneIDi 110751.
KEGGi mmu:110751.
UCSCi uc008mkm.2. mouse. [Q923W9-1 ]

Organism-specific databases

CTDi 80332.
MGIi MGI:1341813. Adam33.

Phylogenomic databases

eggNOGi NOG294463.
GeneTreei ENSGT00760000118888.
HOGENOMi HOG000230883.
HOVERGENi HBG006978.
InParanoidi Q923W9.
KOi K08616.
OMAi LPCAGRD.
OrthoDBi EOG7F7W89.
PhylomeDBi Q923W9.
TreeFami TF314733.

Miscellaneous databases

NextBioi 364593.
PROi Q923W9.
SOURCEi Search...

Gene expression databases

Bgeei Q923W9.
CleanExi MM_ADAM33.
ExpressionAtlasi Q923W9. baseline and differential.
Genevestigatori Q923W9.

Family and domain databases

Gene3Di 3.40.390.10. 1 hit.
4.10.70.10. 1 hit.
InterProi IPR006586. ADAM_Cys-rich.
IPR001762. Blood-coag_inhib_Disintegrin.
IPR018358. Disintegrin_CS.
IPR000742. EG-like_dom.
IPR013032. EGF-like_CS.
IPR024079. MetalloPept_cat_dom.
IPR001590. Peptidase_M12B.
IPR002870. Peptidase_M12B_N.
[Graphical view ]
Pfami PF08516. ADAM_CR. 1 hit.
PF00200. Disintegrin. 1 hit.
PF01562. Pep_M12B_propep. 1 hit.
PF01421. Reprolysin. 1 hit.
[Graphical view ]
PRINTSi PR00289. DISINTEGRIN.
SMARTi SM00608. ACR. 1 hit.
SM00050. DISIN. 1 hit.
SM00181. EGF. 1 hit.
[Graphical view ]
SUPFAMi SSF57552. SSF57552. 1 hit.
PROSITEi PS50215. ADAM_MEPRO. 1 hit.
PS00427. DISINTEGRIN_1. 1 hit.
PS50214. DISINTEGRIN_2. 1 hit.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 1 hit.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and characterization of novel mouse and human ADAM33s with potential metalloprotease activity."
    Yoshinaka T., Nishii K., Yamada K., Sawada H., Nishiwaki E., Smith K., Yoshino K., Ishiguro H., Higashiyama S.
    Gene 282:227-236(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
    Tissue: Brain.
  2. Karkkainen I., Liehu M.A., Huovila A.-P.J.
    Submitted (JAN-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Strain: NMRI.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "M-ADAM33 cloned from mouse embryo day 11 cDNA library."
    Smith K.M., Alfandari D., White J.M., Sutherland A.E., DeSimone D.W.
    Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 113-797 (ISOFORM 1).
    Strain: Swiss Webster / NIH.
  6. Lubec G., Kang S.U.
    Submitted (APR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 562-570 AND 780-797, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: C57BL/6.
    Tissue: Brain.

Entry informationi

Entry nameiADA33_MOUSE
AccessioniPrimary (citable) accession number: Q923W9
Secondary accession number(s): Q8R5G5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: July 27, 2011
Last modified: October 29, 2014
This is version 118 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3