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Protein

Disintegrin and metalloproteinase domain-containing protein 33

Gene

Adam33

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi134Zinc; in inhibited formBy similarity1
Metal bindingi346Zinc; catalyticBy similarity1
Active sitei347PROSITE-ProRule annotation1
Metal bindingi350Zinc; catalyticBy similarity1
Metal bindingi356Zinc; catalyticBy similarity1

GO - Molecular functioni

  • metalloendopeptidase activity Source: UniProtKB
  • zinc ion binding Source: UniProtKB

GO - Biological processi

  • proteolysis Source: UniProtKB
  • regulation of cell proliferation Source: UniProtKB
  • regulation of cytokine biosynthetic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiM12.244.

Names & Taxonomyi

Protein namesi
Recommended name:
Disintegrin and metalloproteinase domain-containing protein 33 (EC:3.4.24.-)
Short name:
ADAM 33
Gene namesi
Name:Adam33
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1341813. Adam33.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini30 – 702ExtracellularSequence analysisAdd BLAST673
Transmembranei703 – 723HelicalSequence analysisAdd BLAST21
Topological domaini724 – 797CytoplasmicSequence analysisAdd BLAST74

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 29Sequence analysisAdd BLAST29
PropeptideiPRO_000002914430 – 204By similarityAdd BLAST175
ChainiPRO_0000029145205 – 797Disintegrin and metalloproteinase domain-containing protein 33Add BLAST593

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi110N-linked (GlcNAc...)Sequence analysis1
Glycosylationi232N-linked (GlcNAc...)Sequence analysis1
Glycosylationi277N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi321 ↔ 405By similarity
Disulfide bondi361 ↔ 389By similarity
Disulfide bondi362 ↔ 372By similarity
Glycosylationi408N-linked (GlcNAc...)Sequence analysis1
Glycosylationi449N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi476 ↔ 496By similarity
Glycosylationi645N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi654 ↔ 664By similarity
Disulfide bondi658 ↔ 670By similarity
Disulfide bondi672 ↔ 681By similarity

Post-translational modificationi

The precursor is cleaved by a furin endopeptidase.By similarity

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiQ923W9.
PRIDEiQ923W9.

PTM databases

PhosphoSitePlusiQ923W9.

Expressioni

Gene expression databases

BgeeiENSMUSG00000027318.
CleanExiMM_ADAM33.
ExpressionAtlasiQ923W9. baseline and differential.
GenevisibleiQ923W9. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000105861.

Structurei

3D structure databases

ProteinModelPortaliQ923W9.
SMRiQ923W9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini211 – 410Peptidase M12BPROSITE-ProRule annotationAdd BLAST200
Domaini418 – 504DisintegrinPROSITE-ProRule annotationAdd BLAST87
Domaini650 – 682EGF-likePROSITE-ProRule annotationAdd BLAST33

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi132 – 139Cysteine switchBy similarity8

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi504 – 649Cys-richAdd BLAST146
Compositional biasi767 – 772Poly-Pro6

Domaini

The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

Sequence similaritiesi

Contains 1 disintegrin domain.PROSITE-ProRule annotation
Contains 1 EGF-like domain.PROSITE-ProRule annotation
Contains 1 peptidase M12B domain.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3607. Eukaryota.
ENOG410XX2M. LUCA.
GeneTreeiENSGT00760000118888.
HOGENOMiHOG000230883.
HOVERGENiHBG006978.
InParanoidiQ923W9.
KOiK08616.
OMAiHFLPCAG.
OrthoDBiEOG091G01NX.
PhylomeDBiQ923W9.
TreeFamiTF314733.

Family and domain databases

Gene3Di3.40.390.10. 1 hit.
4.10.70.10. 1 hit.
InterProiIPR006586. ADAM_Cys-rich.
IPR018358. Disintegrin_CS.
IPR001762. Disintegrin_dom.
IPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR024079. MetalloPept_cat_dom.
IPR001590. Peptidase_M12B.
IPR002870. Peptidase_M12B_N.
[Graphical view]
PfamiPF08516. ADAM_CR. 1 hit.
PF00200. Disintegrin. 1 hit.
PF01562. Pep_M12B_propep. 1 hit.
PF01421. Reprolysin. 1 hit.
[Graphical view]
PRINTSiPR00289. DISINTEGRIN.
SMARTiSM00608. ACR. 1 hit.
SM00050. DISIN. 1 hit.
[Graphical view]
SUPFAMiSSF57552. SSF57552. 1 hit.
PROSITEiPS50215. ADAM_MEPRO. 1 hit.
PS00427. DISINTEGRIN_1. 1 hit.
PS50214. DISINTEGRIN_2. 1 hit.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 1 hit.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q923W9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGSRCGRPGG SPVLLLLPLL LPSCPLRSAR MFPGNAHGEL VTPHWILEGR
60 70 80 90 100
LWLKVTLEEP ILKPDSVLVA LEAEGQDLLL ELEKKHKLLA PGYTETHYRP
110 120 130 140 150
DGHPVVLSPN HTDHCQYHGR VRGFRESWVV LSTCSGMSGL IVLSSKVSYY
160 170 180 190 200
LQPRTPGDTK DFPTHEIFRM EQLFTWRGVQ RDKNSQYKAG MASLPHVPQS
210 220 230 240 250
RVRREARRSP RYLELYIVAD HTLFLLQHQN LNHTRQRLLE VANCVDQILR
260 270 280 290 300
TLDIQLVLTG LEVWTEQDLS RITQDANETL WAFLQWRRGV WARRPHDSTQ
310 320 330 340 350
LLTGRTFQGT TVGLAPVEGI CRAESSGGVS TDHSELPIGT AATMAHEIGH
360 370 380 390 400
SLGLHHDPEG CCVQADAEQG GCVMEAATGH PFPRVFSACS RRQLRTFFRK
410 420 430 440 450
GGGPCLSNTS APGLLVLPSR CGNGFLEAGE ECDCGSGQKC PDPCCFAHNC
460 470 480 490 500
SLRAGAQCAH GDCCARCLLK SAGTPCRPAA TDCDLPEFCT GTSPYCPADV
510 520 530 540 550
YLLDGSPCAE GRGYCLDGWC PTLEQQCQQL WGPGSKPAPE PCFQQMNSMG
560 570 580 590 600
NSQGNCGQDH KGSFLPCAQR DALCGKLLCQ GGEPNPLVPH IVTMDSTILL
610 620 630 640 650
EGREVVCRGA FVLPDSHLDQ LDLGLVEPGT GCGPRMVCQD RHCQNATSQE
660 670 680 690 700
LERCLTACHN GGVCNSNRNC HCAAGWAPPF CDKPGLGGSV DSGPAQSANR
710 720 730 740 750
DAFPLAMLLS FLLPLLPGAG LAWCYYQLPT FCHRRGLCCR RDPLWNRDIP
760 770 780 790
LGSVHPVEFG SIITGEPSPP PPWTSCQQRS HPPSLDLLSD PANSELT
Length:797
Mass (Da):86,971
Last modified:July 27, 2011 - v3
Checksum:iB332EA909514E626
GO
Isoform 2 (identifier: Q923W9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     637-662: Missing.

Show »
Length:771
Mass (Da):84,115
Checksum:iDAFFBA1D4416B5D2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti68L → P in BAB84336 (PubMed:11814695).Curated1
Sequence conflicti68L → P in BAB84337 (PubMed:11814695).Curated1
Sequence conflicti319 – 326GICRAESS → DMPRGELSF in AAK67164 (Ref. 5) Curated8
Sequence conflicti725Missing in AAK67164 (Ref. 5) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_005496637 – 662Missing in isoform 2. 1 PublicationAdd BLAST26

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB059633 mRNA. Translation: BAB84337.1.
AB059632 mRNA. Translation: BAB84336.1.
AF472524 mRNA. Translation: AAL79834.1.
AL833771 Genomic DNA. Translation: CAM18045.1.
CH466519 Genomic DNA. Translation: EDL28296.1.
AF386072 mRNA. Translation: AAK67164.1.
CCDSiCCDS16753.1. [Q923W9-1]
CCDS50716.1. [Q923W9-2]
RefSeqiNP_291093.2. NM_033615.3. [Q923W9-1]
UniGeneiMm.108550.

Genome annotation databases

EnsembliENSMUST00000052104; ENSMUSP00000052486; ENSMUSG00000027318. [Q923W9-2]
ENSMUST00000110232; ENSMUSP00000105861; ENSMUSG00000027318. [Q923W9-1]
ENSMUST00000183552; ENSMUSP00000139344; ENSMUSG00000027318. [Q923W9-1]
GeneIDi110751.
KEGGimmu:110751.
UCSCiuc008mkm.2. mouse. [Q923W9-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB059633 mRNA. Translation: BAB84337.1.
AB059632 mRNA. Translation: BAB84336.1.
AF472524 mRNA. Translation: AAL79834.1.
AL833771 Genomic DNA. Translation: CAM18045.1.
CH466519 Genomic DNA. Translation: EDL28296.1.
AF386072 mRNA. Translation: AAK67164.1.
CCDSiCCDS16753.1. [Q923W9-1]
CCDS50716.1. [Q923W9-2]
RefSeqiNP_291093.2. NM_033615.3. [Q923W9-1]
UniGeneiMm.108550.

3D structure databases

ProteinModelPortaliQ923W9.
SMRiQ923W9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000105861.

Protein family/group databases

MEROPSiM12.244.

PTM databases

PhosphoSitePlusiQ923W9.

Proteomic databases

PaxDbiQ923W9.
PRIDEiQ923W9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000052104; ENSMUSP00000052486; ENSMUSG00000027318. [Q923W9-2]
ENSMUST00000110232; ENSMUSP00000105861; ENSMUSG00000027318. [Q923W9-1]
ENSMUST00000183552; ENSMUSP00000139344; ENSMUSG00000027318. [Q923W9-1]
GeneIDi110751.
KEGGimmu:110751.
UCSCiuc008mkm.2. mouse. [Q923W9-1]

Organism-specific databases

CTDi80332.
MGIiMGI:1341813. Adam33.

Phylogenomic databases

eggNOGiKOG3607. Eukaryota.
ENOG410XX2M. LUCA.
GeneTreeiENSGT00760000118888.
HOGENOMiHOG000230883.
HOVERGENiHBG006978.
InParanoidiQ923W9.
KOiK08616.
OMAiHFLPCAG.
OrthoDBiEOG091G01NX.
PhylomeDBiQ923W9.
TreeFamiTF314733.

Miscellaneous databases

PROiQ923W9.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000027318.
CleanExiMM_ADAM33.
ExpressionAtlasiQ923W9. baseline and differential.
GenevisibleiQ923W9. MM.

Family and domain databases

Gene3Di3.40.390.10. 1 hit.
4.10.70.10. 1 hit.
InterProiIPR006586. ADAM_Cys-rich.
IPR018358. Disintegrin_CS.
IPR001762. Disintegrin_dom.
IPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR024079. MetalloPept_cat_dom.
IPR001590. Peptidase_M12B.
IPR002870. Peptidase_M12B_N.
[Graphical view]
PfamiPF08516. ADAM_CR. 1 hit.
PF00200. Disintegrin. 1 hit.
PF01562. Pep_M12B_propep. 1 hit.
PF01421. Reprolysin. 1 hit.
[Graphical view]
PRINTSiPR00289. DISINTEGRIN.
SMARTiSM00608. ACR. 1 hit.
SM00050. DISIN. 1 hit.
[Graphical view]
SUPFAMiSSF57552. SSF57552. 1 hit.
PROSITEiPS50215. ADAM_MEPRO. 1 hit.
PS00427. DISINTEGRIN_1. 1 hit.
PS50214. DISINTEGRIN_2. 1 hit.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 1 hit.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiADA33_MOUSE
AccessioniPrimary (citable) accession number: Q923W9
Secondary accession number(s): Q8R5G5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 131 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.