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Protein

Trimethylguanosine synthase

Gene

Tgs1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the 2 serial methylation steps for the conversion of the 7-monomethylguanosine (m7G) caps of snRNAs and snoRNAs to a 2,2,7-trimethylguanosine (m(2,2,7)G) cap structure. The enzyme is specific for guanine, and N7 methylation must precede N2 methylation. Hypermethylation of the m7G cap of U snRNAs leads to their concentration in nuclear foci, their colocalization with coilin and the formation of canonical Cajal bodies (CBs). Plays a role in transcriptional regulation (By similarity).By similarity1 Publication

Catalytic activityi

S-adenosyl-L-methionine + m7G(5')pppR-RNA = S-adenosyl-L-homocysteine + m(2,7)G(5')pppR-RNA.
S-adenosyl-L-methionine + m(2,7)G(5')pppR-RNA = S-adenosyl-L-homocysteine + m(2,2,7)G(5')pppR-RNA.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei711 – 7111S-adenosyl-L-methionineBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

ReactomeiR-MMU-191859. snRNP Assembly.
R-MMU-400206. Regulation of lipid metabolism by Peroxisome proliferator-activated receptor alpha (PPARalpha).

Names & Taxonomyi

Protein namesi
Recommended name:
Trimethylguanosine synthase (EC:2.1.1.-)
Alternative name(s):
Nuclear receptor coactivator 6-interacting protein
PRIP-interacting protein with methyltransferase motif
Short name:
PIMT
Short name:
PIPMT
Gene namesi
Name:Tgs1
Synonyms:Ncoa6ip, Pimt
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:2151797. Tgs1.

Subcellular locationi

  • Cytoplasm By similarity
  • NucleusCajal body 1 Publication
  • Nucleusnucleolus By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 853853Trimethylguanosine synthasePRO_0000204469Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei61 – 611PhosphothreonineBy similarity
Modified residuei81 – 811PhosphoserineBy similarity
Modified residuei85 – 851PhosphoserineBy similarity
Modified residuei92 – 921PhosphoserineBy similarity
Modified residuei139 – 1391PhosphoserineBy similarity
Modified residuei144 – 1441PhosphotyrosineBy similarity
Modified residuei152 – 1521PhosphoserineCombined sources
Modified residuei405 – 4051PhosphoserineBy similarity
Modified residuei431 – 4311PhosphoserineCombined sources
Modified residuei572 – 5721PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ923W1.
MaxQBiQ923W1.
PaxDbiQ923W1.
PeptideAtlasiQ923W1.
PRIDEiQ923W1.

PTM databases

iPTMnetiQ923W1.
PhosphoSiteiQ923W1.

Expressioni

Tissue specificityi

Ubiquitously expressed.1 Publication

Gene expression databases

BgeeiENSMUSG00000028233.
GenevisibleiQ923W1. MM.

Interactioni

Subunit structurei

May form homooligomers. Interacts with CREBBP/CBP, EED/WAIT1, EP300/P300, NCOA6/PRIP, PPARBP/PBP and SMN (By similarity).By similarity

Protein-protein interaction databases

BioGridi228104. 20 interactions.
IntActiQ923W1. 21 interactions.
STRINGi10090.ENSMUSP00000054112.

Structurei

3D structure databases

ProteinModelPortaliQ923W1.
SMRiQ923W1. Positions 626-836.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi612 – 62211Lys-richAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2730. Eukaryota.
ENOG410XRUD. LUCA.
GeneTreeiENSGT00390000018056.
HOGENOMiHOG000154561.
HOVERGENiHBG059797.
InParanoidiQ923W1.
KOiK14292.
OMAiMNTRNKV.
OrthoDBiEOG091G0DYO.
PhylomeDBiQ923W1.
TreeFamiTF313065.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR019012. RNA_cap_Gua-N2-MeTrfase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF09445. Methyltransf_15. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.

Sequencei

Sequence statusi: Complete.

Q923W1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCCEKWNHVA EMLLFIEDRE EEYKILCLCS RAFVEDRKLY NLGLKGYYVK
60 70 80 90 100
SSGNNAGDQG TEEEEDGHSN GTAESHSPNE SDLDSEAKLM RSMGLPIQFG
110 120 130 140 150
RMSSHENFEM SMNARNKAKV KQKRRKHQKR YLDEMVRESW RNDYEEDDLV
160 170 180 190 200
VSDDPSSVEH CENNRTCEIQ SKAGSEVENL PVENTLAPKL EVPENWEKYW
210 220 230 240 250
NEYGEGLLWQ SWQEKYPDQT LSSEPWNLPD TKEEWEQHYS QLYWYYLEQF
260 270 280 290 300
QYWEAQGWTF TASQNCDKDV YTSHTEVDQN AESSLKADVM TFSSSPNIVE
310 320 330 340 350
DEIPGSNDND HNEIITAINN ITVSAEKVEQ SQLDSSQHCD EPLSEITGKE
360 370 380 390 400
CPASGGSDSC NGTPKENDIS ENRSSDQPAK ELQESSGTNK GKHRPHHNGA
410 420 430 440 450
DGHESDDDPP EHKPSKVKRS HELDVDENPD SEVDDNGFLL GFKHGSGQKY
460 470 480 490 500
GGIPNFSHRQ VRYLEKNVKY KSKYLDLRKQ MPVKSKHILF TEDSGKPFVV
510 520 530 540 550
CKSKVRSKVE KFLKWVNERV DEETSQDSLS QNKMQDTCTS SDSEEQDMSL
560 570 580 590 600
EKADNLMETR DPEPEKCQII SSATELEAEK SEVGSLVATV PENCSTEEIP
610 620 630 640 650
NSPHAETEVE IKKKKKKNKN KKINDLPPEI ASVPELAKYW AQRYRLFSRF
660 670 680 690 700
DDGIKLDKEG WFSVTPEKIA EHIAGRVSQA FRCDVVVDAF CGVGGNTIQF
710 720 730 740 750
ALTGKRVIAI DIDPVKIDLA RNNAEVYGIA DKIEFICGDF LLLAPCLKAD
760 770 780 790 800
VVFLSPPWGG PDYATAETFD IRTMMSPDGF EIFRLSQKIT NNIVYFLPRN
810 820 830 840 850
ADIDQVASLA GLGGQVEIEQ NFLNNKLKTI TAYFGDLIRR PALLKTSTSE

AEV
Length:853
Mass (Da):96,790
Last modified:April 3, 2007 - v2
Checksum:iA69974BE660DA3AF
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti512 – 5121F → L in AAH75728 (PubMed:15489334).Curated
Sequence conflicti633 – 6331V → G in AAK84355 (PubMed:11517327).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF389908 mRNA. Translation: AAK84355.1.
AL732617 Genomic DNA. Translation: CAM17201.1.
BC026368 mRNA. Translation: AAH26368.1.
BC058183 mRNA. Translation: AAH58183.1.
BC075728 mRNA. Translation: AAH75728.1.
CCDSiCCDS17938.1.
RefSeqiNP_473430.3. NM_054089.4.
UniGeneiMm.171323.

Genome annotation databases

EnsembliENSMUST00000052712; ENSMUSP00000054112; ENSMUSG00000028233.
GeneIDi116940.
KEGGimmu:116940.
UCSCiuc008rwi.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF389908 mRNA. Translation: AAK84355.1.
AL732617 Genomic DNA. Translation: CAM17201.1.
BC026368 mRNA. Translation: AAH26368.1.
BC058183 mRNA. Translation: AAH58183.1.
BC075728 mRNA. Translation: AAH75728.1.
CCDSiCCDS17938.1.
RefSeqiNP_473430.3. NM_054089.4.
UniGeneiMm.171323.

3D structure databases

ProteinModelPortaliQ923W1.
SMRiQ923W1. Positions 626-836.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi228104. 20 interactions.
IntActiQ923W1. 21 interactions.
STRINGi10090.ENSMUSP00000054112.

PTM databases

iPTMnetiQ923W1.
PhosphoSiteiQ923W1.

Proteomic databases

EPDiQ923W1.
MaxQBiQ923W1.
PaxDbiQ923W1.
PeptideAtlasiQ923W1.
PRIDEiQ923W1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000052712; ENSMUSP00000054112; ENSMUSG00000028233.
GeneIDi116940.
KEGGimmu:116940.
UCSCiuc008rwi.2. mouse.

Organism-specific databases

CTDi96764.
MGIiMGI:2151797. Tgs1.

Phylogenomic databases

eggNOGiKOG2730. Eukaryota.
ENOG410XRUD. LUCA.
GeneTreeiENSGT00390000018056.
HOGENOMiHOG000154561.
HOVERGENiHBG059797.
InParanoidiQ923W1.
KOiK14292.
OMAiMNTRNKV.
OrthoDBiEOG091G0DYO.
PhylomeDBiQ923W1.
TreeFamiTF313065.

Enzyme and pathway databases

ReactomeiR-MMU-191859. snRNP Assembly.
R-MMU-400206. Regulation of lipid metabolism by Peroxisome proliferator-activated receptor alpha (PPARalpha).

Miscellaneous databases

ChiTaRSiTgs1. mouse.
PROiQ923W1.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028233.
GenevisibleiQ923W1. MM.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR019012. RNA_cap_Gua-N2-MeTrfase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF09445. Methyltransf_15. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTGS1_MOUSE
AccessioniPrimary (citable) accession number: Q923W1
Secondary accession number(s): A2AJF7
, Q6DI60, Q6PEA7, Q8R0W9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: April 3, 2007
Last modified: September 7, 2016
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.